Inoculation device for strain cultivation

Abstract

The utility model relates to the technical field of hypsizygus marmoreus liquid strain culture, in particular to an inoculation device for strain cultivation, which comprises a support rod, an inoculation head connected to the left end of the support rod, a handle arranged at the right end of the support rod, a limiting rubber plug sleeved on the ring surface of the support rod, and a limiting baffle plate fixedly arranged on the ring surface of the support rod and close to the right end. By means of the design, the problems that in the hypsizygus marmoreus liquid strain culture process, hyphae are manually divided through an inoculation hook and transferred into a culture bottle, operation is tedious, the speed is low, and meanwhile the pollution risk is high are solved. According to the inoculation hook, the structural design of an original inoculation hook is improved, the inoculation process of a hypsizygus marmoreus liquid strain is greatly simplified, the inoculation speed and the inoculation efficiency are improved, meanwhile, hyphae of hypsizygus marmoreus can be prevented from being in contact with a bottle opening in the inoculation process, the pollution risk in the inoculation process is reduced, and the safety of the hypsizygus marmoreus is improved. The powerful technical support is provided for the culture of the hypsizygus marmoreus liquid strain.

Description

Technical Field

[0001] The utility model relates to the technical field of seafood mushroom liquid strain culture, in particular to an inoculation device for strain cultivation. Background Art

[0002] Seafood mushrooms are low-temperature grass-growing fungi or wood-growing white-rot fungi. They have high nutritional and medicinal value. They are white in color, thick in flesh, delicate in taste, fragrant and delicious. The amino acids in the protein of seafood mushrooms are complete, including 8 essential amino acids for the human body, and they also contain several polysaccharides. The hot water and organic solvent extracts of their fruiting bodies have the function of removing free radicals from the human body. Therefore, eating seafood mushrooms regularly has the effects of anti-cancer, cancer prevention, improving immunity, preventing aging and prolonging life. It is an edible fungus with high nutritional and medicinal value. It is white in color, thick in flesh, delicate in taste, fragrant and delicious. The optimum temperature for the growth of mycelium is 24-26℃, and the optimum temperature for the growth of fruiting bodies is 8-18℃. Cultivated in autumn and winter, the age of the fungus is about 50 days, the growth cycle is 100 days, and the bioconversion rate is 60-80%.

[0003] Liquid strains are obtained by deep culture technology using liquid culture medium under specific environmental and nutritional conditions. Compared with solid strains, liquid strains have the advantages of fast growth, uniform mushroom age, uniform fruiting, easy management, low strain cost, and convenient inoculation. In recent years, they have been widely used in the cultivation of edible fungi.

[0004] In the prior art, during the cultivation process of the seafood mushroom liquid strain, the seafood mushroom mycelium in the culture test tube is manually divided into rice grain sizes using an inoculation hook, and then transferred into a culture bottle containing a culture medium in batches, with three to four seafood mushroom mycelium in each bottle, for further cultivation. However, during this process, the inoculation hook and the seafood mushroom mycelium cannot contact the bottle mouth to prevent the breeding of miscellaneous bacteria and pollution, resulting in the inoculation personnel needing to be highly concentrated during the operation, the operation is inconvenient, the inoculation speed is slow, and the cultivation efficiency of the seafood mushroom liquid strain is affected. In order to solve the above problems, the utility model provides an improved inoculation device for the cultivation of seafood mushroom liquid strains. Utility Model Content

[0005] In view of the existing deficiencies, the utility model provides an inoculation device for fungus cultivation, which solves the problems raised in the above-mentioned background technology.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] An inoculation device for bacterial cultivation comprises a support rod, wherein the left end of the support rod is connected to an inoculation head, the right end of the support rod is provided with a handle, the support rod annular surface is sleeved with a limit rubber plug, a limit baffle is fixedly provided on the support rod annular surface near the right end, and a return spring is sleeved on the support rod between the limit baffle and the limit rubber plug.

[0008] Furthermore, the inoculation head consists of a fixed rod, a collecting blade and a connecting head. The connecting head is screwed together with the left end of the support rod through a thread. A fixed rod is fixed on the left side of the connecting head. A plurality of groups of collecting blades with a spoon-shaped structure are arranged on the lower side of the annular surface of the fixed rod.

[0009] Furthermore, the limiting rubber plug is configured as a conical structure, and the diameter of its left end face is smaller than the diameter of its right end face, a positioning slot with a right angle structure is provided on the ring surface of the limiting rubber plug, and an integrated retaining ring is provided on the ring surface of the limiting rubber plug near the right end face.

[0010] Furthermore, a layer of cotton pad is pasted on the ring surface of the limiting rubber plug.

[0011] Furthermore, a sliding sleeve is fixedly provided at the inner center of the limiting rubber plug, and the sliding sleeve is sleeved on the support rod.

[0012] Furthermore, a limiting sliding block is arranged on the inner wall of the sliding sleeve, and a limiting sliding groove for accommodating the limiting sliding block is opened on the upper side of the annular surface of the support rod, and the limiting sliding block is inserted into the limiting sliding groove.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] 1. The utility model improves the structural design of the original inoculation hook, greatly simplifies the inoculation process of seafood mushroom liquid spawn, improves the inoculation speed and efficiency, and can avoid the contact between seafood mushroom mycelium and the bottle mouth during the inoculation process, reducing the risk of contamination during the inoculation process, and provides strong technical support for the cultivation of seafood mushroom liquid spawn.

[0015] 2. In the utility model, multiple groups of spoon-shaped blades are arranged on the lower side of the fixed rod ring surface. The spoon-shaped blades help to better wrap and cut the mycelium, ensuring that the right amount of mycelium can be accurately obtained each time. At the same time, the arrangement of multiple groups of blades facilitates the simultaneous collection of multiple mycelium, improving the mycelium inoculation speed and efficiency. This design facilitates the collection of seafood mushroom mycelium through the inoculation head, can accurately divide and pick up the mycelium, and greatly improves the accuracy and efficiency of inoculation.

[0016] 3. The limiting rubber plug is set to a conical structure, and the diameter of its left end face is smaller than the diameter of the right end face. This design enables the limiting rubber plug to be inserted into culture containers of different calibers and provide limiting support for the entire device. At the same time, it can also provide a tighter sealing effect to prevent the entry of miscellaneous bacteria.

[0017] 4. A right-angle positioning slot is provided on the ring surface of the limiting rubber plug. The positioning slot can form a limiting structure at the tube mouth when inserted into the test tube, ensuring the airtight connection between the limiting rubber plug and the test tube, effectively preventing the inoculation head from contacting the inner wall of the test tube during the collection process. An integrated retaining ring is provided on the ring surface of the limiting rubber plug near the right end surface. The retaining ring can form a limiting structure at the bottle mouth when the mycelium is transferred to the culture bottle, ensuring the airtight connection between the limiting rubber plug and the culture bottle, and effectively preventing the inoculation head from contacting the inner wall of the culture bottle.

[0018] 5. A layer of cotton pad is pasted on the ring surface of the limiting rubber plug. The cotton pad has good water absorption and can absorb disinfectants such as alcohol. The limiting rubber plug is disinfected with disinfectant to ensure that the limiting rubber plug is always in a sterile state. At the same time, the cotton pad is soft and can better adapt to the opening shape of the culture container, thereby providing a tighter seal.

[0019] 6. The limit slider and the limit groove can ensure the stable sliding of the sliding sleeve on the support rod, and at the same time play a role of limit guide for the sliding of the limit rubber plug, preventing the limit rubber plug from sliding excessively and falling off the support rod, and at the same time making the sliding of the limit rubber plug on the support rod more stable without shaking or deviation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0021] Figure 2 It is a front view of the utility model.

[0022] Figure 3 It is a structural schematic diagram of the support rod in the utility model.

[0023] Figure 4 It is a structural schematic diagram of the limiting rubber plug in the utility model.

[0024] Figure 5 It is a cross-sectional view of the limiting rubber plug in the utility model.

[0025] In the figure: 1. Inoculation head; 1.1. Fixing rod; 1.2. Taking blade; 1.3. Connecting head; 2. Support rod; 3. Limit rubber plug; 4. Handle; 5. Reset spring; 6. Limit slide groove; 7. Limit baffle; 8. Baffle ring; 9. Positioning slot; 10. Cotton pad; 11. Sleeve; 12. Limit slider. DETAILED DESCRIPTION

[0026] The following will be combined with the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] Example:

[0028] like Figures 1 to 5 As shown, an inoculation device for cultivating spawn includes a support rod 2, an inoculation head 1 is connected to the left end of the support rod 2, a handle 4 is arranged at the right end of the support rod 2, a limiting rubber plug 3 is sleeved on the annular surface of the support rod 2, a limiting baffle 7 is fixedly arranged on the annular surface of the support rod 2 near the right end, and a return spring 5 is sleeved on the support rod 2 between the limiting baffle 7 and the limiting rubber plug 3. This design solves the problem that in the process of culturing liquid spawn of seafood mushroom, the mycelium is manually divided and transferred into a culture bottle using an inoculation hook, which is cumbersome and slow to operate and has a high risk of contamination.

[0029] The inoculation head 1 is composed of a fixed rod 1.1, a collection blade 1.2 and a connector 1.3. The connector 1.3 is screwed and connected to the left end of the support rod 2 through a thread. This connection method is both stable and easy to replace or repair. When the inoculation head 1 is damaged or needs to be cleaned, it can be easily removed from the support rod 2. A fixed rod 1.1 is fixed on the left side of the connector 1.3. The main function of the fixed rod 1.1 is to provide a stable platform for installing and supporting the collection blade 1.2. A plurality of groups of spoon-shaped collection blades 1.2 are arranged on the lower side of the annular surface of the fixed rod 1.1. The spoon-shaped collection blades 1.2 help to better wrap and cut the mycelium, ensuring that the right amount of mycelium can be accurately obtained each time. At the same time, the setting of multiple groups of collection blades 1.2 facilitates the simultaneous collection of multiple mycelium, thereby improving the inoculation speed and efficiency of the mycelium. The design facilitates the collection of seafood mushroom mycelium through the inoculation head 1, and can accurately divide and pick up the mycelium, greatly improving the accuracy and efficiency of inoculation.

[0030] The limiting rubber plug 3 is set to a conical structure, and the diameter of its left end face is smaller than the diameter of the right end face. This design enables the limiting rubber plug 3 to be inserted into culture containers of different calibers and provide limiting support for the entire device. At the same time, it can also provide a tighter sealing effect to prevent the entry of miscellaneous bacteria. A positioning card slot 9 with a right-angle structure is provided on the ring surface of the limiting rubber plug 3. The positioning card slot 9 can form a limiting structure at the mouth of the tube when inserted into the test tube, ensuring the airtight connection between the limiting rubber plug 3 and the test tube, and effectively preventing the inoculation head 1 from contacting the inner wall of the test tube during the collection process. A retaining ring 8 of an integrated structure is provided on the ring surface of the limiting rubber plug 3 near the right end face. The retaining ring 8 can form a limiting structure at the mouth of the bottle when the mycelium is transferred to the culture bottle, ensuring the airtight connection between the limiting rubber plug 3 and the culture bottle, and effectively preventing the inoculation head 1 from contacting the inner wall of the culture bottle.

[0031] A layer of cotton pad 10 is pasted on the annular surface of the limiting rubber plug 3. The cotton pad 10 has good water absorption and can absorb disinfectants such as alcohol. The limiting rubber plug 3 is disinfected with the disinfectant to ensure that the limiting rubber plug 3 is always in a sterile state. At the same time, the cotton pad 10 is soft in texture and can better adapt to the opening shape of the culture container, thereby providing a tighter seal.

[0032] A sliding sleeve 11 is fixed at the inner center of the limiting rubber plug 3, and the sliding sleeve 11 is sleeved on the support rod 2. The setting of the sliding sleeve 11 allows the limiting rubber plug 3 to slide smoothly along the support rod 2, which can reduce the friction resistance between the limiting rubber plug 3 and the support rod 2, making the limiting rubber plug 3 slide more smoothly.

[0033] A limit slider 12 is provided on the inner wall of the sliding sleeve 11, and a limit slot 6 for accommodating the limit slider 12 is provided on the upper side of the annular surface of the support rod 2, and the limit slider 12 is inserted into the limit slot 6. The limit slider 12 cooperates with the limit slot 6 to ensure the stable sliding of the sliding sleeve 11 on the support rod 2, and at the same time, it plays a role of limit guide for the sliding of the limit rubber plug 3, preventing the limit rubber plug 3 from sliding excessively and falling off the support rod 2, and at the same time, making the sliding of the limit rubber plug 3 on the support rod 2 more stable without shaking or deviation.

[0034] The working principle of the inoculation device for cultivating fungus is as follows: in actual use, first disinfect the inoculation head 1 and the support rod 2, then sprinkle alcohol or other disinfectants on the cotton pad 10 outside the limiting rubber plug 3, and use the disinfectant to disinfect the limiting rubber plug 3 to ensure that the limiting rubber plug 3 is always in a sterile state. The operator holds the handle 4 and extends the inoculation head 1 into the test tube to aim at the seafood mushroom hyphae. In this process, the positioning slot 9 on the limiting rubber plug 3 is stuck at the mouth of the test tube, and the support rod 2 is supported by the limiting rubber plug 3 to ensure that the support rod 2 and the inoculation head 1 are always on the axis of the test tube, effectively preventing the inoculation head 1 from contacting the inner wall of the test tube. Push the support rod 2 toward the inside of the test tube, and rotate the support rod 2 at the same time, so that the blade 1.2 on the inoculation head 1 cuts an appropriate amount of hyphae. The blade 1.2 with a spoon-shaped structure can accurately wrap and cut the hyphae, ensuring that the amount obtained each time is moderate, and multiple groups of blades 1.2 can simultaneously take multiple portions of hyphae, thereby increasing the inoculation speed of the hyphae. Transfer the collected mycelium to the culture bottle. During this process, the retaining ring 8 of the limiting rubber plug 3 will fit at the bottle mouth to ensure that the inoculation head 1 does not touch the inner wall of the culture bottle, thereby avoiding the risk of contamination. Then remove the joint from the culture bottle and repeat the above steps to perform the inoculation operation. When the inoculation head 1 is removed from the culture bottle, the reset spring 5 pushes the limiting rubber plug 3 back to the initial position to prepare for the next operation.

[0035] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not limitations on the implementation methods of the present invention. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made on the basis of the above description. It is impossible to list all the implementation methods here. All obvious changes or modifications derived from the technical solution of the present invention are still within the protection scope of the present invention.

Claims

1. An inoculation device for bacterial cultivation, comprising a support rod (2), characterized in that: The left end of the support rod (2) is connected to an inoculation head (1), the right end of the support rod (2) is provided with a handle (4), the support rod (2) is provided with a limit rubber plug (3) on the annular surface, a limit baffle (7) is fixed on the annular surface of the support rod (2) near the right end, and a return spring (5) is provided on the support rod (2) between the limit baffle (7) and the limit rubber plug (3).

2. The inoculation device for bacterial cultivation according to claim 1, characterized in that: The inoculation head (1) is composed of a fixed rod (1.1), a collecting blade (1.2) and a connecting head (1.3); the connecting head (1.3) is screwed together with the left end of the support rod (2) via a thread; the fixed rod (1.1) is fixedly disposed on the left side of the connecting head (1.3); and a plurality of groups of collecting blades (1.2) with a spoon-shaped structure are disposed on the lower side of the annular surface of the fixed rod (1.1).

3. The inoculation device for bacterial cultivation according to claim 1, characterized in that: The limiting rubber plug (3) is configured as a conical structure, and the diameter of its left end face is smaller than the diameter of its right end face. A positioning slot (9) with a right-angle structure is provided on the annular surface of the limiting rubber plug (3), and a retaining ring (8) with an integrated structure is provided on the annular surface of the limiting rubber plug (3) near the right end face.

4. The inoculation device for bacterial cultivation according to claim 3, characterized in that: A layer of cotton pad (10) is adhered to the annular surface of the limiting rubber plug (3).

5. The inoculation device for bacterial cultivation according to claim 1, characterized in that: A sliding sleeve (11) is fixedly arranged at the inner center of the limiting rubber plug (3), and the sliding sleeve (11) is sleeved on the support rod (2).

6. The inoculation device for bacterial cultivation according to claim 5, characterized in that: A limiting slide block (12) is arranged on the inner wall of the sliding sleeve (11), and a limiting slide groove (6) for accommodating the limiting slide block (12) is provided on the upper side of the annular surface of the support rod (2), and the limiting slide block (12) is inserted into the limiting slide groove (6).

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