Extraction device with mixing function

Abstract

The utility model discloses an extraction device with a mixing function, and relates to the technical field of cell exosome extraction devices. Comprising a bottom plate, a mixing and stirring structure and a filtering structure, the mixing and stirring structure is fixedly connected to the left side of the upper surface of the bottom plate, the filtering structure is fixedly connected to the right side of the upper surface of the bottom plate, a stirring motor drives a rotating shaft to rotate, and the rotating shaft rotates to drive a telescopic air cylinder and a telescopic rod to rotate, so that a stirring rod is driven to rotate; the cell exosome and the diluent in the mixing box are mixed and stirred, a telescopic cylinder drives a telescopic rod to move up and down, so that a stirring rod is driven to move up and down, the stirring range of the stirring rod is enlarged, and the cell exosome and the diluent can be mixed more uniformly; the diluted cell exosome is guided into the filtering box body through the second liquid inlet pipe and the first electromagnetic valve, the diluted cell exosome is filtered through the first filtering screen and the second filtering screen, and the filtered cell exosome can be discharged through the liquid outlet pipe.

Description

Technical Field

[0001] The utility model relates to the technical field of extracellular vesicle extraction devices, in particular to an extraction device with a mixing function. Background Art

[0002] Exosomes are tiny membrane vesicles secreted by cells, which contain cell-specific proteins, lipids and nucleic acids. They can be used for gene therapy, as well as for the diagnosis and treatment of cancer. In gene therapy, the delivery of genetic material is an important link, and the membrane vesicles of exosomes have the functions of transportation and delivery. Gene carriers are encapsulated and delivered through the membrane vesicles of exosomes, which generally do not harm the genetic material, thereby effectively exchanging genetic information between cells, reprogramming host cells, and conducting intercellular communication. Exosomes exist in human body fluids, and many cells can secrete exosomes. Both normal cells and cells with pathological conditions can secrete, and these secreted exosomes have the physiological functions or pathological changes of the source cells. By detecting exosomes, the situation of the source cells can be clearly understood. If the exosomes contain a large amount of specific miRNA, it can be diagnosed that the source cell secreting the exosomes belongs to a tumor cell. And then used for the diagnosis of cancer. When extracting exosomes, because the exosomes have a certain viscosity, the exosomes need to be diluted, but the existing extraction equipment cannot dilute and stir the exosomes. Therefore, those skilled in the art provide an extraction device with a mixing function to solve the problems raised in the above background technology. Utility Model Content

[0003] The purpose of the utility model is to provide an extraction device with a mixing function to solve the problems raised in the above background technology.

[0004] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0005] An extraction device with a mixing function comprises a bottom plate, a mixing and stirring structure and a filtering structure, wherein the left side of the upper surface of the bottom plate is fixedly connected with the mixing and stirring structure, the right side of the upper surface of the bottom plate is fixedly connected with the filtering structure, the mixing and stirring structure comprises: a mixing box, a liquid inlet pipe 1, a liquid inlet pipe cover, a stirring motor, a rotating shaft, a telescopic cylinder, a telescopic rod and a stirring rod, the left side of the upper surface of the bottom plate is fixedly connected with the mixing box, the left side of the upper surface of the mixing box is fixedly connected with the liquid inlet pipe 1, the upper end of the surface of the liquid inlet pipe 1 is threadedly connected with the liquid inlet pipe cover, the middle of the upper surface of the mixing box is fixedly connected with the stirring motor, and the bottom surface of the stirring motor is movably connected with a rotating shaft The invention relates to a filter structure comprising: a filter box body, a box door, a box door handle, two liquid inlet pipes, one solenoid valve, one fixed plate, one card slot, one card-joining frame, one filter screen, two fixed plates, two card slots, two card-joining frames, two filter screens, a liquid outlet pipe, two solenoid valves and a close-fitting colloid. The filter box body is fixedly connected to the right side of the upper surface of the bottom plate.

[0006] Preferably, a second liquid inlet pipe is fixedly connected to the middle of the upper surface of the filter box, and one end of the second liquid inlet pipe away from the filter box is connected to the interior of the mixing box.

[0007] Preferably: a solenoid valve 1 is fixedly connected to the surface of the liquid inlet pipe 2, and a fixing plate 1 is fixedly connected to the upper ends of the left and right surfaces inside the filter box, and the number of the fixing plates 1 is two groups.

[0008] Preferably: a card slot 1 is provided on one side surface corresponding to each other of the two sets of fixing plates 1, and a card connection frame 1 is movably connected between the one side surfaces corresponding to each other of the two sets of fixing plates 1 and inside the card slot 1.

[0009] Preferably: a filter screen 1 is fixedly connected inside the snap-fit ​​frame 1, and a fixing plate 2 is fixedly connected on the left and right side surfaces inside the filter box and at the lower end of the fixing plate 1, and the number of the fixing plates 2 is two groups.

[0010] Preferably: a second card slot is provided on one side surface corresponding to each other of the two sets of fixing plates 2, and a second card-joining frame is movably connected between the one side surfaces corresponding to each other of the two sets of fixing plates 2 and inside the second card slot.

[0011] Preferably, a second filter screen is fixedly connected inside the second snap-fit ​​frame, a liquid outlet pipe is fixedly connected to the lower end on the right side surface of the filter box, and a second solenoid valve is fixedly connected to the surface of the liquid outlet pipe.

[0012] Preferably, a close-fitting colloid is fixedly connected to the front surface of the filter box, a box door is movably connected to the front side of the filter box via a hinge, and a box door handle is fixedly connected to the right side of the front surface of the box door.

[0013] Compared with the prior art, the utility model has the following beneficial effects: extracellular vesicles and diluent are added into the mixing box through the liquid inlet pipe 1 at the upper end of the mixing box, the liquid inlet pipe cover is covered on the upper end of the liquid inlet pipe 1, the rotating shaft is driven to rotate by the stirring motor, the rotation of the rotating shaft drives the telescopic cylinder and the telescopic rod to rotate, thereby driving the stirring rod to rotate, and the extracellular vesicles and diluent in the mixing box are mixed and stirred, the telescopic rod is driven up and down by the telescopic cylinder, and the up and down movement of the telescopic rod drives the stirring rod at the lower end of the telescopic rod to move up and down, thereby increasing the stirring range of the stirring rod, and the extracellular vesicles and diluent can be mixed more evenly, and then the liquid inlet pipe 2 and the electromagnetic Valve 1 introduces the diluted exosomes into the interior of the filter box, and the diluted exosomes are filtered through filter screen 1 and filter screen 2. The filtered exosomes can be discharged through the liquid outlet pipe, and the box door is pulled by the box door handle, so that the snap-on frame 1 and the snap-on frame 2 can be replaced. The function of the close-fitting colloid is to make the connection between the box door and the filter box tighter, thereby preventing the exosomes from leaking out, which solves the above-mentioned problem that when extracting exosomes, due to the certain viscosity of the exosomes, the exosomes need to be diluted, but the existing extraction equipment cannot dilute and stir the exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of an extraction device with mixing function.

[0015] Figure 2 It is a structural schematic diagram of a partial structural plane structure of an extraction device with a mixing function.

[0016] Figure 3 It is a structural schematic diagram of a partial filtering structure in an extraction device with mixing function.

[0017] Figure 4 The figure is a schematic diagram of the internal structure of a filter box in an extraction device with a mixing function.

[0018] Figure 5 The present invention is a structural schematic diagram of a snap-fit ​​frame body and a filter screen in an extraction device with a mixing function.

[0019] Figure 6 The present invention is a structural schematic diagram of a second clamping frame and a second filtering screen in an extraction device with a mixing function.

[0020] In the figure: 1-bottom plate, 2-mixing box, 201-liquid inlet pipe 1, 202-liquid inlet pipe cover, 3-stirring motor, 301-rotating shaft, 4-telescopic cylinder, 401-telescopic rod, 402-stirring rod, 5-filter box, 501-box door, 502-box door handle, 6-liquid inlet pipe 2, 601-solenoid valve 1, 7-fixed plate 1, 701-card slot 1, 8-card-connected frame 1, 801-filter screen 1, 9-fixed plate 2, 901-card slot 2, 10-card-connected frame 2, 1001-filter screen 2, 11-liquid outlet pipe, 1101-solenoid valve 2, 12-closed colloid. DETAILED DESCRIPTION

[0021] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0022] See also Figures 1 to 6In the embodiment of the utility model, an extraction device with a mixing function includes a bottom plate 1, a mixing and stirring structure and a filtering structure. The left side of the upper surface of the bottom plate 1 is fixedly connected with the mixing and stirring structure, and the right side of the upper surface of the bottom plate 1 is fixedly connected with the filtering structure. The mixing and stirring structure includes: a mixing box 2, a liquid inlet pipe 201, a liquid inlet pipe cover 202, a stirring motor 3, a rotating shaft 301, a telescopic cylinder 4, a telescopic rod 401 and a stirring rod 402. The left side of the upper surface of the bottom plate 1 is fixedly connected with the mixing box 2, the left side of the upper surface of the mixing box 2 is fixedly connected with the liquid inlet pipe 201, the upper end of the surface of the liquid inlet pipe 201 is threadedly connected with the liquid inlet pipe cover 202, the middle of the upper surface of the mixing box 2 is fixedly connected with the stirring motor 3, and the stirring motor 3 is movably connected with a rotating shaft 301, one end of the rotating shaft 301 away from the stirring motor 3 passes through the upper surface of the mixing box 2 and is located inside the mixing box 2, a telescopic cylinder 4 is fixedly connected on the bottom surface of the rotating shaft 301 and located inside the mixing box 2, a telescopic rod 401 is movably connected on the bottom surface of the telescopic cylinder 4, a stirring rod 402 is fixedly connected on the bottom surface of the telescopic rod 401 and located inside the mixing box 2, and the filtering structure includes: a filtering box body 5, a box door 501, a box door handle 502, a liquid inlet pipe 6, a solenoid valve 601, a fixing plate 7, a slot 701, a snap-on frame 8, a filtering screen 801, a fixing plate 9, a slot 901, a snap-on frame 10, and a filtering screen 1001 , liquid outlet pipe 11, solenoid valve 1101 and close contact colloid 12, the right side of the upper surface of the bottom plate 1 is fixedly connected with a filter box 5, the middle of the upper surface of the filter box 5 is fixedly connected with a liquid inlet pipe 6, the end of the liquid inlet pipe 6 away from the filter box 5 is connected with the interior of the mixing box 2, and the surface of the liquid inlet pipe 6 is fixedly connected with a solenoid valve 601, and the upper ends of the left and right side surfaces inside the filter box 5 are fixedly connected with fixed plates 7, and the number of the fixed plates 7 is two groups, and the two groups of fixed plates 7 have a slot 701 on the side surfaces corresponding to each other, and a snap-in frame 8 is movably connected between the side surfaces corresponding to each other and located inside the slot 701, and the inside of the snap-in frame 8 is fixedly connected with a filter. Screen 1 801, fixed plates 2 9 are fixedly connected on the left and right side surfaces inside the filter box 5 and located at the lower end of the fixed plate 1 7, the number of the fixed plates 2 9 is two groups, and the two groups of fixed plates 2 9 are provided with a card slot 2 901 on the side surfaces corresponding to each other, and a card connection frame 2 10 is movably connected between the side surfaces corresponding to each other of the two groups of fixed plates 2 9 and located in the card slot 2 901, and a filter screen 2 1001 is fixedly connected inside the card connection frame 2 10, a liquid outlet pipe 11 is fixedly connected to the lower end of the right side surface of the filter box 5, and a solenoid valve 2 1101 is fixedly connected to the surface of the liquid outlet pipe 11, a close-fitting colloid 12 is fixedly connected to the front side surface of the filter box 5, and a box door 501 is movably connected to the front side of the filter box 5 through a hinge,A door handle 502 is fixedly connected to the right side of the front surface of the door 501.

[0023] The working principle of the utility model is:

[0024] The utility model relates to an extraction device with a mixing function. In a specific use process, first, extracellular vesicles and diluent are added into the interior of the mixing box 2 through a liquid inlet pipe 201 at the upper end of the mixing box 2, and a liquid inlet pipe cover 202 is covered on the upper end of the liquid inlet pipe 201. The rotating shaft 301 is driven to rotate by a stirring motor 3, and the rotation of the rotating shaft 301 drives the telescopic cylinder 4 and the telescopic rod 401 to rotate, thereby driving the stirring rod 402 to rotate, and the extracellular vesicles and diluent in the mixing box 2 are mixed and stirred. The telescopic rod 401 is driven up and down by the telescopic cylinder 4, and the telescopic rod 401 moves up and down, thereby driving the stirring rod 402 at the lower end of the telescopic rod 401 to rotate upward. The stirring rod 402 moves downward, thereby increasing the stirring range of the stirring rod 402, so that the extracellular vesicles and the diluent can be mixed more evenly, and then the diluted extracellular vesicles are introduced into the interior of the filter box 5 through the liquid inlet pipe 2 6 and the solenoid valve 1 601, and the diluted extracellular vesicles are filtered through the filter screen 1 801 and the filter screen 2 1001. The filtered extracellular vesicles can be discharged through the liquid outlet pipe 11, and the box door 501 is pulled by the box door handle 502, so that the card-joined frame 1 8 and the card-joined frame 2 10 can be replaced. The function of the close-fitting colloid 12 is to make the connection between the box door 501 and the filter box 5 tighter, thereby preventing the extracellular vesicles from leaking out.

[0025] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An extraction device with a mixing function, comprising a bottom plate (1), a mixing and stirring structure and a filtering structure, characterized in that: The left side of the upper surface of the bottom plate (1) is fixedly connected with a mixing and stirring structure, and the right side of the upper surface of the bottom plate (1) is fixedly connected with a filtering structure. The mixing and stirring structure comprises: a mixing box (2), a liquid inlet pipe (201), a liquid inlet pipe cover (202), a stirring motor (3), a rotating shaft (301), a telescopic cylinder (4), a telescopic rod (401) and a stirring rod (402). The left side of the upper surface of the bottom plate (1) is fixedly connected with the mixing box (2), the left side of the upper surface of the mixing box (2) is fixedly connected with a liquid inlet pipe (201), the upper end of the surface of the liquid inlet pipe (201) is threadedly connected with the liquid inlet pipe cover (202), the middle of the upper surface of the mixing box (2) is fixedly connected with the stirring motor (3), and the bottom surface of the stirring motor (3) is movably connected with the rotating shaft (301), and the end of the rotating shaft (301) away from the stirring motor (3) passes through the upper surface of the mixing box (2) and is located Inside the mixing box (2), a telescopic cylinder (4) is fixedly connected on the bottom surface of the rotating shaft (301) and located inside the mixing box (2); a telescopic rod (401) is movably connected on the bottom surface of the telescopic cylinder (4); a stirring rod (402) is fixedly connected on the bottom surface of the telescopic rod (401) and located inside the mixing box (2); the filtering structure comprises: a filtering box body (5), a box door (501), a box door handle (502), a second liquid inlet pipe (6), a first solenoid valve (601), a first fixing plate (7), a first clamping groove (701), a first clamping frame body (8), a first filter screen (801), a second fixing plate (9), a second clamping groove (901), a second clamping frame body (10), a second filter screen (1001), a liquid outlet pipe (11), a second solenoid valve (1101) and a close-fitting colloid (12); and the filtering box body (5) is fixedly connected on the right side of the upper surface of the bottom plate (1).

2. The extraction device with mixing function according to claim 1, characterized in that: A second liquid inlet pipe (6) is fixedly connected in the middle of the upper surface of the filter box (5), and one end of the second liquid inlet pipe (6) away from the filter box (5) is connected to the interior of the mixing box (2).

3. The extraction device with mixing function according to claim 1, characterized in that: A solenoid valve (601) is fixedly connected to the surface of the liquid inlet pipe (6), and a fixing plate (7) is fixedly connected to the upper ends of the left and right surfaces inside the filter box (5), and the number of the fixing plates (7) is two groups.

4. The extraction device with mixing function according to claim 3, characterized in that: A card slot 1 (701) is provided on one side surface corresponding to each other of the two sets of fixing plates 1 (7), and a card-joining frame 1 (8) is movably connected between the one side surfaces corresponding to each other of the two sets of fixing plates 1 (7) and located inside the card slot 1 (701).

5. The extraction device with mixing function according to claim 1, characterized in that: A filter screen 1 (801) is fixedly connected inside the snap-fit ​​frame 1 (8), and a fixing plate 2 (9) is fixedly connected on the left and right side surfaces inside the filter box (5) and located at the lower end of the fixing plate 1 (7), and the number of the fixing plates 2 (9) is two groups.

6. The extraction device with mixing function according to claim 5, characterized in that: A second clamping groove (901) is provided on the surfaces of one side corresponding to each other of the two sets of fixing plates (9), and a second clamping frame (10) is movably connected between the surfaces of one side corresponding to each other of the two sets of fixing plates (9) and inside the second clamping groove (901).

7. The extraction device with mixing function according to claim 1, characterized in that: A second filter screen (1001) is fixedly connected inside the second snap-fit ​​frame (10), a liquid outlet pipe (11) is fixedly connected to the lower end on the right side surface of the filter box (5), and a second solenoid valve (1101) is fixedly connected to the surface of the liquid outlet pipe (11).

8. The extraction device with mixing function according to claim 1, characterized in that: A close-fitting colloid (12) is fixedly connected to the front surface of the filter box (5), a box door (501) is movably connected to the front side of the filter box (5) via a hinge, and a box door handle (502) is fixedly connected to the right side of the front surface of the box door (501).

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