Ice bath and fixing device for ultrasonic cell crusher

Abstract

The utility model discloses an ice bath and fixing device for an ultrasonic cell disruptor, which comprises a bottom box matched with a cavity of the ultrasonic cell disruptor and a beaker filled with a sample to be disrupted, the outer side of the bottom box is adhered by using an anti-skid adhesive tape, an acrylic bracket for placing the beaker is arranged in the bottom box, and a clamping groove for fixing the beaker is arranged on the lower layer of the bracket. And other gaps between the beakers and the box body are filled with ice-water mixtures. The part, flush with the beaker, of the acrylic frame fixes the beaker on the upper layer through a gripper. According to the device for cell ultrasonication, the beaker is fixed in various forms such as the bracket, the clamping groove and the grasping clamp, so that the beaker does not topple over in an ice bath. Meanwhile, through the arrangement of the transparent acrylic material, the melting condition of the ice-water mixture can be observed, the ice blocks can be replaced in time to prevent the ice-water mixture from being quickly melted, the ultrasonic crushing work of the cells is facilitated, and the cost is low.

Description

Technical Field

[0001] The utility model relates to the technical field of test equipment, in particular to an ice bath and a fixing device for an ultrasonic cell disruptor. Background Art

[0002] Ultrasonic cell disruptors are common instruments in laboratories. Some ultrasonic samples need to be placed in beakers for ultrasonication due to the amount of samples and the size of the horn. However, during the ultrasonic process, the unstable beaker often moves, causing the horn to collide with the beaker wall and break the beaker, which can easily damage the horn and waste samples. At the same time, the temperature will rise rapidly during the cell disruption process, and the enzyme activity of biological samples such as cells will be affected. Therefore, in order to maintain activity, ice cubes are generally placed at the bottom or around the container containing the sample, but this can easily cause the sample to be unbalanced and affect the sample disruption effect. Utility Model Content

[0003] Purpose of the utility model: The purpose of the utility model is to provide an ice bath and fixing device for an ultrasonic cell disruptor which has strong versatility, good stability, simple structure and easy operation.

[0004] Technical solution: The utility model discloses an ice bath and fixing device for an ultrasonic cell disruptor, comprising a bottom box matching the cavity of the ultrasonic cell disruptor and a beaker containing a sample to be disrupted, wherein a bracket for placing the beaker is provided in the bottom box, wherein the bracket comprises a fixing surface and a bottom surface, wherein the fixing surface is provided with a hole for fixing the beaker, wherein the bottom surface is provided with a groove for stabilizing the bottom of the beaker, and a cooling substance is filled between the bottom box and the beaker; the diameter of the bottom box matches the inner diameter of the cavity of the ultrasonic cell disruptor, and an anti-slip tape is provided on the outer side of the bottom box; grippers for clamping the beaker are provided on the upper parts of both sides of the bracket, and the size of the grippers matches the diameter of the beaker; and the bracket is an acrylic bracket.

[0005] Furthermore, the cooling substance is an ice-water mixture.

[0006] Furthermore, the height of the bottom box is 4 / 5 of the height of the beaker.

[0007] Furthermore, the bottom box is a coverless rectangular box.

[0008] Furthermore, the height of the wide side of the frame is greater than the distance of the upper plane of the frame, and a 5mm gap is left between the bottom surface of the bracket and the inner side of the bottom box.

[0009] Beneficial effects: Compared with the prior art, the utility model has the following advantages: (1) The utility model can be used to fix and ice bath the beaker of an ultrasonic cell disruptor, which can prevent the beaker from moving, avoid the amplitude rod hitting the wall and causing the beaker to break, and reduce the substantial temperature rise caused by the ultrasonic disruption process; (2) The utility model adopts a transparent acrylic material, which can observe the degree of ice melting when multiple samples are ultrasonically treated, and timely pour out part of the liquid and add new ice; (3) The utility model has a simple structure and low production cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0011] Figure 2 It is a top view of the utility model;

[0012] Figure 3 It is a side view of the utility model.

[0013] In the figure: 1. bottom box; 2. acrylic frame; 3. ice-water mixture; 4. beaker; 5. anti-slip tape edging; 6. beaker slot; 7. grab clip. DETAILED DESCRIPTION

[0014] The technical solution of the utility model is further described below in conjunction with the accompanying drawings.

[0015] As shown in the figure, the utility model provides an ice bath and fixing device for an ultrasonic cell disruptor, comprising a bottom box 1 matching the cavity of the ultrasonic cell disruptor and a beaker 4 containing a sample to be disrupted, the bottom box 1 being a coverless rectangular parallelepiped box body, a bracket 2 for placing the beaker 4 being provided in the bottom box 1, the bracket 2 comprising a fixing surface and a bottom surface, the fixing surface being provided with a hole for fixing the beaker, the bottom surface being provided with a groove 6 for stabilizing the bottom of the beaker, and a cooling substance being filled between the bottom box 1 and the beaker 4. The diameter of the bottom box 1 matches the inner diameter of the cavity of the ultrasonic cell disruptor, and a non-slip tape edging 5 is provided on the outside of the bottom box 1. A gripper for clamping the beaker is provided on the upper part of both sides of the bracket 2, and the size of the gripper matches the diameter of the beaker. The cooling substance is an ice-water mixture. The bracket 2 is an acrylic bracket. The gripper 7 is fixed to the bracket 2 by screws. The height of the bottom box 1 is 4 / 5 of the height of the beaker 4. The wide side of the bracket 2 is higher than the bracket fixing surface, and a 5mm gap is left between the bottom surface of the bracket and the inner side of the bottom box.

[0016] When in use, place the beaker 4 containing the sample to be crushed into the hole of the fixed surface and fix it with the gripper 7. Then add the ice cubes made of the ice cube mold and the ice water mixture into the gap between the beaker 4 and the bottom box 1. After filling with ice cubes, put it into the soundproof box of the ultrasonic cell crusher, insert the horn and temperature probe, adjust the ultrasonic height and start ultrasonication. Since the length of the fixing device itself is customized according to the width of the soundproof box and the friction is increased by the use of anti-slip tape, it will not move during the ultrasonic process. At the same time, the transparent acrylic material is used. When multiple samples are ultrasonicated, the degree of melting of the ice cubes can be observed and some liquid can be poured out in time to add new ice cubes.

Claims

1. An ice bath and fixing device for an ultrasonic cell disruptor, characterized in that: The invention comprises a bottom box (1) matched with the cavity of an ultrasonic cell disruptor and a beaker (4) containing a sample to be disrupted. A bracket (2) for placing the beaker (4) is arranged inside the bottom box (1). The bracket (2) comprises a fixing surface and a bottom surface. The fixing surface is provided with a hole for fixing the beaker. The bottom surface is provided with a groove (6) for stabilizing the bottom of the beaker. A cooling substance (3) is filled between the bottom box (1) and the beaker (4). The diameter of the bottom box (1) matches the inner diameter of the cavity of the ultrasonic cell disruptor. The outer side of the bottom box (1) is provided with an anti-slip tape edging (5). The upper parts of both sides of the bracket (2) are provided with gripping clips (7) for clamping the beaker. The size of the gripping clips matches the diameter of the beaker. The bottom box (1) and the bracket (2) are both made of acrylic material.

2. The ice bath and fixing device for ultrasonic cell disruptor according to claim 1, characterized in that: The grabbing clamp (7) is fixed on the bracket (2) by means of screws.

3. The ice bath and fixing device for ultrasonic cell disruptor according to claim 1, characterized in that: The cooling substance (3) is an ice-water mixture.

4. The ice bath and fixing device for ultrasonic cell disruptor according to claim 1, characterized in that: The height of the bottom box (1) is 4 / 5 of the height of the beaker (4).

5. The ice bath and fixing device for ultrasonic cell disruptor according to claim 1, characterized in that: The bottom box (1) is a box body in the shape of a rectangular parallelepiped without a cover.

6. The ice bath and fixing device for ultrasonic cell disruptor according to claim 4, characterized in that: The wide side of the bracket (2) is higher than the bracket fixing surface, and a 5 mm gap is left between the bottom surface of the bracket and the inner side of the bottom box.

Images