Liquid circulation module

Abstract

The utility model discloses a liquid circulation module to solve the problems that an existing cell culture device cannot accurately simulate an in-vivo dynamic circulation process, cross contamination occurs in a conventional cell culture experiment process, and an experiment result is inaccurate. The system comprises two liquid storage tanks, two pressure pumps and a cell culture device which is matched with the two liquid storage tanks for use, during use, liquid is driven by the pressure pump to flow, so that the liquid flows from the first liquid storage tank to the second liquid storage tank after passing through the flowmeter, the cell culture device and the one-way valve according to a set flow rate, liquid is quickly supplemented from the second liquid storage tank to the first liquid storage tank after being cultured for a period of time, and the process is repeated, so that the long-term dynamic circulating culture process of cells is realized; the device is simple in overall structure and convenient to use, the in-vivo dynamic circulation process can be accurately simulated in the cell culture process, cross contamination in the conventional cell culture experiment process is avoided, and therefore the accuracy of the experiment result is improved; the whole cell culture process is in a sealed state, so that the pollution problem is avoided.

Description

Technical Field

[0001] The present application relates to the technical field of liquid circulation, and in particular to a liquid circulation module in a cell culture process. Background Art

[0002] Cell culture is an important tool in modern biological sciences, providing a key window into the study of the mysteries of life. The significance of cell culture is not limited to basic research, it also plays an important role in medicine, drug development and biotechnology.

[0003] Most existing cell culture methods are 2D culture, and the cell culture devices are usually static, that is, the cells are placed in a plate or culture bottle, lacking a flow environment. They cannot well simulate the long-term dynamic circulation of blood in the human body, and cross-contamination is prone to occur during conventional cell culture experiments, thus affecting the accuracy of the experimental results. Utility Model Content

[0004] To this end, the present application provides a liquid circulation module to solve the problem that existing cell culture devices cannot accurately simulate the dynamic circulation process in the body, resulting in cross-contamination in conventional cell culture experimental processes and inaccurate experimental results.

[0005] In order to achieve the above objectives, this application provides the following technical solutions:

[0006] A liquid circulation module, the liquid circulation module is used in conjunction with a cell culture device, the liquid circulation module comprises: a first liquid storage tank, a second liquid storage tank, a first pressure pump, and a second pressure pump; the first liquid storage tank and the second liquid storage tank are respectively provided with a first liquid storage tank cap and a second liquid storage tank cap on their upper parts;

[0007] The first liquid reservoir cap is provided with a first liquid inlet, a first liquid outlet and a first pneumatic joint, and the first pressure pump is connected to the first pneumatic joint via a first air pipe; the second liquid reservoir cap is provided with a second liquid inlet, a second liquid outlet and a second pneumatic joint, and the second pressure pump is connected to the second pneumatic joint via a second air pipe;

[0008] The first liquid inlet is connected to the second liquid outlet through a first pipeline, the first liquid outlet is connected to the second liquid inlet through the cell culture device, and the cell culture device is connected to the first liquid outlet and the second liquid inlet through a second pipeline and a third pipeline respectively.

[0009] Optionally, a flow meter is also provided on the second pipeline.

[0010] Optionally, a first one-way valve and a second one-way valve are respectively provided at the first liquid inlet and the second liquid inlet.

[0011] Optionally, the first liquid reservoir cap and the second liquid reservoir cap are both made of metal.

[0012] Optionally, the first pipe, the second pipe and the third pipe are all capillaries.

[0013] Optionally, a test tube rack is further included for placing the first liquid storage tank and the second liquid storage tank, and the first liquid storage tank and the second liquid storage tank are in the shape of test tube bottles.

[0014] Optionally, the first liquid storage tank and the second liquid storage tank are made of transparent material, and scale lines are provided on their outer surfaces.

[0015] Compared with the prior art, this application has at least the following beneficial effects:

[0016] 1. Based on further analysis and research on the problems of the prior art, the present application provides a liquid circulation module, including two liquid storage tanks, two pressure pumps and a cell culture device used in conjunction with them; when in use, the pressure pump drives the liquid to flow, so that the liquid flows from the first liquid storage tank into the second liquid storage tank through the flow meter, the cell culture device and the one-way valve at a set flow rate. After a period of culture, the liquid is quickly replenished from the second liquid storage tank to the first liquid storage tank, and the cycle is repeated to achieve a dynamic circulation culture process of cells; the liquid storage tank of the present application uses a 15mL liquid storage tank, which can support large-capacity culture fluid circulation culture, and at the same time, it is equipped with a pressure pump to perform long-term circulation perfusion culture on cells. The overall structure is simple and easy to use. During the cell culture process, it can accurately simulate the dynamic circulation process in the body, avoid cross contamination during conventional cell culture experiments, and thus improve the accuracy of the experimental results; the entire cell culture process is in a sealed state, avoiding contamination problems;

[0017] 2. The second pipeline of the present application is also provided with a flow meter, which can accurately measure the delivery velocity and flow rate of the liquid, so as to well control the cell culture process;

[0018] 3. The first and second liquid inlets of the present application are respectively provided with a first one-way valve and a second one-way valve to ensure that liquid can only enter but not exit;

[0019] 4. The present application also includes a test tube rack for placing the first liquid storage tank and the second liquid storage tank, providing a stable platform for cell culture and ensuring the smooth progress of the cell culture process; at the same time, it also effectively utilizes laboratory space. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more intuitively illustrate the prior art and the present application, exemplary drawings are given below. It should be understood that the specific shapes and structures shown in the drawings should not generally be regarded as limiting conditions for implementing the present application; for example, those skilled in the art are capable of easily making conventional adjustments or further optimizations to the addition / reduction / attribution division, specific shapes, positional relationships, connection methods, dimensional ratios, etc. of certain units (components) based on the technical concepts and exemplary drawings disclosed in the present application.

[0021] Figure 1 A schematic diagram of the structure of a liquid circulation module provided in one embodiment of the present application.

[0022] Description of reference numerals:

[0023] 1. Cell culture device;

[0024] 2. First liquid storage tank; 201. First liquid storage tank cap; 202. First pneumatic joint;

[0025] 3. Second liquid storage tank; 301. Second liquid storage tank cap; 302. Second pneumatic joint;

[0026] 4. First pressure pump; 5. Second pressure pump; 6. Flow meter;

[0027] 7. First air pipe; 8. Second air pipe; 9. First pipeline; 10. Second pipeline; 11. Third pipeline. DETAILED DESCRIPTION

[0028] The present application is further described below in detail through specific embodiments in conjunction with the accompanying drawings.

[0029] In the description of this application: unless otherwise specified, the meaning of "plurality" is two or more. The terms "first", "second", "third", etc. in this application are intended to distinguish the objects referred to, and do not have special meanings in terms of technical connotations (for example, they should not be understood as emphasizing the importance or order, etc.). Expressions such as "including", "comprising", "having", etc. also mean "not limited to" (certain units, components, materials, steps, etc.).

[0030] The terms such as "upper", "lower", "left", "right", "middle", etc. cited in this application are usually used to indicate the general relative position relationship for the purpose of intuitive understanding by referring to the drawings, and are not absolute limitations on the position relationship in the actual product.

[0031] The present application aims to solve the problems existing in the prior art and provides a liquid circulation module, such as Figure 1As shown, the liquid circulation module is used in conjunction with a cell culture device 1 for cell culture; the liquid circulation module comprises: a first liquid storage tank 2, a second liquid storage tank 3, a first pressure pump 4, and a second pressure pump 5;

[0032] The first liquid reservoir 2 and the second liquid reservoir 3 are provided with a first liquid reservoir cap 201 and a second liquid reservoir cap 301 on their upper parts respectively; the first liquid reservoir 2 and the second liquid reservoir 3 are used to store culture fluid for culturing cells;

[0033] The first liquid reservoir cap 201 is provided with a first liquid inlet, a first liquid outlet and a first pneumatic joint 202, and the first pressure pump 4 is connected to the first pneumatic joint 202 via a first air pipe 7; the second liquid reservoir cap 301 is provided with a second liquid inlet, a second liquid outlet and a second pneumatic joint 302, and the second pressure pump 5 is connected to the second pneumatic joint 302 via a second air pipe 8; the two pressure pumps can apply different pressures to the liquid in the liquid reservoir, so that the liquid flows out from the corresponding liquid outlet;

[0034] The first liquid inlet is connected to the second liquid outlet through the first pipe 9, the first liquid outlet is connected to the second liquid inlet through the cell culture device 1, and the cell culture device 1 is connected to the first liquid outlet and the second liquid inlet through the second pipe 10 and the third pipe 11 respectively; the liquid inlets of the two liquid storage tanks are connected to the liquid outlets of each other through pipes (capillaries), one way is used for cell culture in conjunction with the cell culture device 1 and the flow meter 6 described below, and the other way is used for fluid replenishment.

[0035] In this embodiment, two 15mL liquid storage tanks can support large-capacity culture fluid circulation culture, and at the same time, a pressure pump is used to perform long-term circulation perfusion culture on cells; the two liquid storage tanks are sealed and connected by a capillary tube, so that the entire cell culture process is in a sealed state to ensure that the circulating liquid is not contaminated; the liquid inlet and outlet (first liquid inlet, first liquid outlet, second liquid inlet and second liquid outlet) all support standard 1 / 16 capillaries, and the connector (connector for liquid inlet and outlet) also uses a universal 1 / 4-28 connector, which is simple and easy to operate.

[0036] Preferably, a flow meter 6 is also provided on the second pipeline 10, which can accurately measure the delivery velocity and flow rate of the liquid, so as to well control the cell culture process.

[0037] Preferably, a first one-way valve and a second one-way valve are respectively provided at the first liquid inlet and the second liquid inlet, and the provision of the first one-way valve and the second one-way valve ensures that liquid can only enter but not exit.

[0038] Preferably, the first liquid reservoir cap 201 and the second liquid reservoir cap 301 are both made of metal.

[0039] Preferably, the first pipe 9 , the second pipe 10 and the third pipe 11 are all capillaries.

[0040] Preferably, the first liquid reservoir 2 and the second liquid reservoir 3 are connected to the first liquid reservoir cap 201 and the second liquid reservoir cap 301 respectively by threads.

[0041] Preferably, a test tube rack is also included, which is used to place the first liquid reservoir 2 and the second liquid reservoir 3, and the first liquid reservoir 2 and the second liquid reservoir 3 are in the shape of test tube bottles; the setting of the test tube rack provides a stable platform for cell culture, ensuring that the liquid reservoirs are in a suitable position and can be firmly placed on it, preventing accidental movement of the equipment during operation, thereby affecting the cell culture process; at the same time, it also effectively utilizes laboratory space.

[0042] Further preferably, the first liquid storage tank 2 and the second liquid storage tank 3 are made of transparent material, and scale lines are provided on their outer surfaces to facilitate observation of the liquid capacity.

[0043] The use principle of the above embodiment is as follows:

[0044] The first pressure pump 4 drives the liquid in the first liquid storage tank 2 to flow out from the first liquid outlet at a set flow rate, and flows into the second liquid storage tank 3 after passing through the flow meter 6, the cell culture device 1, and the second one-way valve in sequence; after the cells are cultured for a period of time, the liquid is quickly replenished from the second liquid storage tank 3 to the first liquid storage tank 2, specifically by driving the liquid flow through the second pressure pump 5, so that the culture fluid in the second liquid storage tank 3 flows into the first liquid storage tank 2, and this cycle is repeated to realize the dynamic circulation culture process of cells.

[0045] The technical features of the above embodiments may be arbitrarily combined (as long as there is no contradiction in the combination of these technical features). To make the description concise, not all possible combinations of the technical features in the above embodiments are described; these embodiments that are not explicitly written should also be considered to be within the scope of this specification.

Claims

1. A liquid circulation module, characterized in that: The liquid circulation module is used in conjunction with the cell culture device, and the liquid circulation module includes: a first liquid storage tank, a second liquid storage tank, a first pressure pump, and a second pressure pump; the first liquid storage tank and the second liquid storage tank are respectively provided with a first liquid storage tank cap and a second liquid storage tank cap on their upper parts; The first liquid reservoir cap is provided with a first liquid inlet, a first liquid outlet and a first pneumatic joint, and the first pressure pump is connected to the first pneumatic joint via a first air pipe; the second liquid reservoir cap is provided with a second liquid inlet, a second liquid outlet and a second pneumatic joint, and the second pressure pump is connected to the second pneumatic joint via a second air pipe; The first liquid inlet is connected to the second liquid outlet through a first pipeline, the first liquid outlet is connected to the second liquid inlet through the cell culture device, and the cell culture device is connected to the first liquid outlet and the second liquid inlet through a second pipeline and a third pipeline respectively.

2. The liquid circulation module according to claim 1, characterized in that: The second pipeline is also provided with a flow meter.

3. The liquid circulation module according to claim 1 or 2, characterized in that: A first one-way valve and a second one-way valve are respectively disposed at the first liquid inlet and the second liquid inlet.

4. The liquid circulation module according to claim 1, characterized in that: The first liquid storage tank cap and the second liquid storage tank cap are both made of metal.

5. The liquid circulation module according to claim 1, characterized in that: The first pipe, the second pipe and the third pipe are all capillaries.

6. The liquid circulation module according to claim 1, characterized in that: It also includes a test tube rack for placing the first liquid storage tank and the second liquid storage tank, and the first liquid storage tank and the second liquid storage tank are in the shape of a test tube bottle.

7. The liquid circulation module according to claim 1 or 6, characterized in that: The first liquid storage tank and the second liquid storage tank are made of transparent materials, and scale lines are arranged on the outer surfaces thereof.

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