Isothermal amplification reaction anti-pollution test strip sleeve

Abstract

The utility model discloses an isothermal amplification reaction anti-pollution test strip sleeve which comprises a reaction tube, a test strip, a transparent tube and a tube cover, the test strip is mounted in an inner cavity of the transparent tube, the tube cover is mounted at the top end of the transparent tube in a threaded manner, and the upper end of the test strip is connected with the inner top surface of the tube cover; according to the anti-pollution test strip sleeve for the isothermal amplification reaction, a test strip is clamped and mounted in the tube cover and the transparent tube, then after reaction of reaction liquid in the reaction tube is finished, the conical tube at the lower end of the transparent tube is hermetically inserted into an opening of the reaction tube, air is prevented from entering the reaction tube, then the tube cover is screwed downwards in a spiral manner, and the test strip penetrates out of an outlet of the conical tube downwards; and the test strip is immersed into the reaction liquid after the reaction is finished, detection is performed, and the detection result of the test strip is observed, so that the test strip and the reaction liquid are in a sealed state in the reaction process and the result reading process, and therefore, pollution is avoided in the whole isothermal amplification reaction process, and the detection result is more accurate.

Description

Technical Field

[0001] The utility model relates to the technical field of constant temperature amplification, in particular to a contamination-proof test paper strip sleeve for constant temperature amplification reaction. Background Art

[0002] In recent years, with the rapid development of molecular biology technology, constant temperature amplification technology has been applied to many fields such as agriculture, food safety, medicine and transgenic detection. Compared with other nucleic acid amplification technologies, constant temperature amplification technology has the advantages of being fast, efficient and specific, and does not require special equipment. Constant temperature amplification technology is usually combined with test strip detection technology to detect the amplification results.

[0003] At present, in the process of using a test strip to detect a constant temperature amplification reaction solution, the test strip is usually directly inserted into a reaction tube for constant temperature amplification. During this process, the opening of the reaction tube and the surface of the test strip are directly exposed to the air, that is, the reaction solution will come into contact with various substances in the air, which can easily cause the reaction solution and the air to react, thereby causing contamination of the reaction solution. This article aims to propose a test strip sleeve that can avoid contamination of the reaction solution throughout the entire process. Utility Model Content

[0004] The utility model aims to solve the problem in the prior art that the opening of the reaction tube for constant temperature amplification and the surface of the test strip are directly exposed to the air, which easily causes the contamination of the reaction liquid, and proposes a constant temperature amplification reaction anti-contamination test strip sleeve.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A contamination-proof test strip sleeve for isothermal amplification reaction, comprising a reaction tube and a test strip, wherein the lower end of the test strip is inserted into the inner cavity of the reaction tube, and further comprising:

[0007] A transparent tube, wherein the test strip is installed in the inner cavity of the transparent tube;

[0008] The tube cover is threadably mounted on the top of the transparent tube, and the upper end of the test strip is connected to the inner top surface of the tube cover.

[0009] Preferably, an outer periphery of the upper end of the transparent tube is provided with an external thread, and the external thread is threadedly connected to the inner wall of the tube cover.

[0010] Preferably, a clamping column is provided in the tube cover, and the lower end of the clamping column is connected to the top end of the test strip through an anti-slip clamping groove.

[0011] Preferably, the lower end of the transparent tube is fixedly connected to a conical tube.

[0012] Preferably, the lower end outlet of the conical shrink tube is larger than the width of the test strip.

[0013] Preferably, the outer periphery of the tube cover is provided with anti-slip grooves.

[0014] Compared with the prior art, the utility model provides a constant temperature amplification reaction anti-contamination test strip sleeve, which has the following beneficial effects:

[0015] The contamination-proof test strip sleeve for constant temperature amplification reaction clamps and installs the test strip in the tube cover and the transparent tube, and then after adding reaction liquid into the reaction tube, the conical tube at the lower end of the transparent tube is sealed and inserted into the opening of the reaction tube to prevent air from entering the reaction tube, and no cover opening operation is required from the start of the reaction to the completion of result observation; after the reaction is completed, the tube cover is screwed downward, the test strip is passed downward through the outlet of the conical tube, immersed in the reaction liquid for finishing the reaction, and detection is performed, and the detection result of the test strip is observed, so as to ensure that the test strip and the reaction liquid are in a sealed state during the reaction process and when the result is read, thereby achieving the constant temperature amplification reaction and avoiding contamination throughout the process, so that the detection result is more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a structural schematic diagram of a constant temperature amplification reaction anti-pollution test strip sleeve proposed by the utility model;

[0017] Figure 2 The utility model discloses an exploded view of a contamination-proof test strip sleeve for a constant temperature amplification reaction.

[0018] In the figure: 1. reaction tube; 2. reaction cover; 3. transparent tube; 4. tube cover; 5. conical tube; 6. test strip; 7. clamping column; 8. anti-slip clamping groove; 9. external thread. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0020] In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating directions or positional relationships, are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0021] Reference Figure 1-2A constant temperature amplification reaction anti-contamination test strip sleeve comprises a reaction tube 1 and a test strip 6. The upper end of the reaction tube 1 is connected with a reaction cover 2 via a PP soft connection. A transparent tube 3 is arranged on the periphery of the test strip 6. An external thread 9 is fixedly arranged on the periphery of the top end of the transparent tube 3. A tube cover 4 is threadedly installed on the top end of the transparent tube 3 via the external thread 9.

[0022] In this embodiment, a circle of densely arranged anti-skid patterns is provided on the periphery of the tube cover 4, and the anti-skid patterns are used to increase the friction of the tube cover 4 to facilitate the rotation of the tube cover 4. A conical tube 5 is fixedly connected to the lower end of the tube cover 4, and the inner diameter of the conical tube 5 gradually decreases from top to bottom, and the lower end opening of the conical tube 5 is larger than the width of the test strip 6, that is, the width of the test strip 6 is smaller than the inner diameters of the conical tube 5 and the transparent tube. Secondly, the outer diameter of the lower end of the conical tube 5 is smaller than the inner diameter of the opening of the reaction tube 1, which is conducive to guiding the transparent tube 3 to extend into the reaction tube 1. The outer diameter of the upper end of the conical tube 5 is larger than the inner diameter of the opening of the reaction tube 1, and is used to seal and block the opening of the reaction tube 1.

[0023] In this embodiment, the reaction tube 1, the transparent tube 3 and the conical tube 5 are all made of highly transparent materials, and the color change of the internal test strip 6 can be intuitively observed. The test strip 6 is raised and lowered in the inner cavity of the transparent tube 3. A clamping column 7 is fixedly installed on the inner top surface of the tube cover 4. The lower end of the clamping column 7 is provided with an anti-slip clamping groove 8. The clamping column 7 is made of highly elastic plastic. The top end of the test strip 6 is clamped and installed in the anti-slip clamping groove 8 in the clamping column 7, that is, the test strip 6 and the tube cover 4 are connected.

[0024] In the present invention, the process of using the test strip 6 to detect the reaction liquid in the reaction tube 1 is as follows:

[0025] 1) Open the anti-skid clamping groove 8 in the clamping column 7, and coaxially clamp the head end of the test strip 6 in the anti-skid clamping groove 8, then insert the end of the test strip 6 into the transparent tube 3, and screw the tube cover 4 onto the external thread 9 at the top of the transparent tube 3. Before testing, there is no need to screw the end of the test strip 6 out of the outlet of the tapered tube 5;

[0026] 2) Open the reaction cover 2 and add the reaction solution into the reaction tube 1;

[0027] 3) The tapered tube 5 at the lower end of the transparent tube 3 is sealed and inserted into the opening of the reaction tube 1, that is, the lower end of the tapered tube 5 blocks the opening of the reaction tube 1 to prevent air from entering the reaction tube 1;

[0028] 4) Screw the tube cover 4 downward, pass the test strip 6 connected to the clamping column 7 at the lower end of the tube cover 4 downward through the outlet of the conical tube 5, immerse it in the reaction solution where the reaction is finished, perform the test, and observe the test result of the test strip 6.

[0029] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A contamination-proof test strip sleeve for a constant temperature amplification reaction, comprising a reaction tube (1) and a test strip (6), wherein the lower end of the test strip (6) is inserted into the inner cavity of the reaction tube (1), characterized in that: Also includes: A transparent tube (3), the test strip (6) being installed in the inner cavity of the transparent tube (3), and the lower end of the transparent tube (3) being fixedly connected to a conical tube (5); The tube cover (4) is threadably mounted on the top of the transparent tube (3), and the upper end of the test strip (6) is connected to the inner top surface of the tube cover (4).

2. The isothermal amplification reaction anti-pollution test strip sleeve according to claim 1, characterized in that: An external thread (9) is provided on the periphery of the upper end of the transparent tube (3), and the external thread (9) is threadedly connected to the inner wall of the tube cover (4).

3. The isothermal amplification reaction anti-pollution test strip sleeve according to claim 1, characterized in that: A clamping column (7) is arranged inside the tube cover (4), and the lower end of the clamping column (7) is connected to the top end of the test strip (6) via an anti-slip clamping groove (8).

4. The isothermal amplification reaction anti-pollution test strip sleeve according to claim 1, characterized in that: The lower end outlet of the conical tube (5) is larger than the width of the test strip (6).

5. The isothermal amplification reaction anti-pollution test strip sleeve according to claim 1, characterized in that: The outer periphery of the tube cover (4) is provided with anti-slip grooves.

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