Detection kit based on flow cytometry

Abstract

The utility model discloses a detection kit based on flow cytometry, which comprises a kit main body, a bottom plate is fixed at the upper end position in the kit main body, a rotating disc capable of rotating is arranged at the upper end position of the bottom plate, and a flow cytometry module is arranged on the rotating disc. Bottle caps are installed at the upper ends of the antibody bottle, the washing buffer solution bottle, the stationary liquid bottle, the penetrating fluid bottle and the calibration micro-bead bottle, the reagent bottle is inserted into the bottle body insertion cylinder, a plurality of rubber balls are distributed in the bottle body insertion cylinder, the rubber balls can rotate when the reagent bottle is inserted or taken, and the reagent bottle is separated from the bottle body insertion cylinder. In this way, the reagent bottles can be taken more smoothly, the angle of the bottle body inserting cylinder can be changed along with force, the angle of the bottle body inserting cylinder can be changed through the rotating device, and after external force disappears, the torsion spring enables the bottle body inserting cylinder to face the upper position again, so that the next storage of the reagent bottles cannot be affected; and a worker can smoothly take or store the reagent bottle.

Description

Technical Field

[0001] The utility model belongs to the technical field related to a detection kit based on flow cytometry, and specifically relates to a detection kit based on flow cytometry. Background Art

[0002] Flow cytometry-based detection kits are kits used for flow cytometer detection. These kits contain fluorescent markers for labeling cell surface or internal molecules so that the flow cytometer can analyze and count different types of cells. These kits are usually used to study cell surface markers, cell cycle analysis, cell apoptosis and other studies. Common flow cytometry kits include cell surface marker detection reagents, cell apoptosis detection reagents, cell cycle detection reagents, etc.

[0003] When the test kit is stored in the reagent bottle, when the staff takes the reagent bottle, the reagent bottle is inserted too tightly, it will be inconvenient to take it, which may easily cause the reagent bottle to break, thus causing safety hazards. Utility Model Content

[0004] The purpose of the utility model is to provide a detection kit based on flow cytometry to solve the problem proposed in the above background technology that when the detection kit stores reagent bottles, when the staff takes the reagent bottles, the reagent bottles are inserted too tightly, which makes it inconvenient to take them, which easily causes the reagent bottles to break, thereby causing safety hazards.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a detection kit based on flow cytometry, comprising a kit body, wherein a bottom plate is fixed at the upper end position inside the kit body, a rotatable rotating disk is installed at the upper end position of the bottom plate, the rotating disk rotates with the central axis at the lower end position as the center of the circle, a rotating device is installed at the upper end position of the rotating disk, a torsion spring is sleeved at the outer wall position of the rotating device, a bottle body insert is installed at the upper end position of the rotating device, a plurality of rubber balls are distributed at the internal position of the bottle body insert, a plurality of the bottle body inserts are provided, and an antibody bottle, a washing buffer bottle, a fixative bottle, a permeate bottle and a calibration microbead bottle are respectively inserted at the internal positions of the plurality of the bottle body inserts, and a bottle cap is installed at the upper end position of the antibody bottle, the washing buffer bottle, the fixative bottle, the permeate bottle and the calibration microbead bottle.

[0006] Preferably, fluorescently labeled monoclonal antibodies are stored in the interior of the antibody bottle, and a buffer for washing sample cells is stored in the interior of the washing buffer bottle.

[0007] Preferably, a solution for fixing cells is stored in the interior of the fixative bottle, and a solution for increasing the permeability of cell membranes is stored in the interior of the permeate bottle.

[0008] Preferably, the calibration microbead bottle stores microbeads for flow cytometer calibration and calibration, and the antibody bottle, washing buffer bottle, fixative bottle, permeate bottle and calibration microbead bottle are all inserted into the inner position of the bottle body insert.

[0009] Preferably, the inner position of the bottle body insert tube causes the rubber ball to rotate when the reagent bottle is inserted, and the rubber ball fits tightly with the reagent bottle.

[0010] Preferably, the bottle insert can change its angle through a rotating device, and the torsion spring makes the bottle insert always face upward after the external force disappears.

[0011] Preferably, the rotating disk enables the bottle inserting tube to rotate, and the rotation of the rotating disk takes the central axis as the center of the circle.

[0012] Preferably, the central axis enables the rotating disk to be mounted on a bottom plate, and the bottom plate is fixed at an internal position of the reagent kit body.

[0013] Compared with the prior art, the utility model provides a detection kit based on flow cytometry, which has the following beneficial effects:

[0014] 1. The reagent bottle of the device is inserted into the inner position of the bottle insert tube. There are multiple rubber balls distributed in the inner position of the bottle insert tube. When inserting or taking out, the rubber balls will rotate, so that it is smoother to take out, and the bottle insert tube will change its angle as the force is applied. The angle of the bottle insert tube can be changed by the rotating device, and after the external force disappears, the torsion spring makes the bottle insert tube face the upper position again, which will not affect the next storage of the reagent bottle. The staff can smoothly complete the process of taking out or storing the reagent bottle.

[0015] 2. The bottle insert can be rotated at any angle through the rotating disk, which makes it convenient for the staff to find a suitable position. When the rotating disk rotates, the central axis rotates at the upper end of the bottom plate. The bottle insert can be adjusted to a suitable angle by simply rotating the disk. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The utility model is a schematic diagram of the structure of a detection kit based on flow cytometry.

[0017] Figure 2 The utility model is a schematic diagram of a top view of the structure of a detection kit based on flow cytometry.

[0018] Figure 3 The utility model is a schematic diagram of a bottle body insert tube structure of a detection kit based on flow cytometry.

[0019] Figure 4 The utility model is a schematic diagram of the internal structure of a bottom plate of a detection kit based on flow cytometry.

[0020] In the figure: 1. Kit body; 2. Antibody bottle; 3. Washing buffer bottle; 4. Fixative bottle; 5. Permeate bottle; 6. Calibration bead bottle; 7. Bottle cap; 8. Rubber ball; 9. Bottle insert; 10. Torsion spring; 11. Rotating device; 12. Rotating disk; 13. Bottom plate; 14. Center axis. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0022] The utility model provides Figure 1-4 A detection kit based on flow cytometry is shown, comprising a kit body 1, wherein a bottom plate 13 is fixed at the upper end of the inside of the kit body 1, a rotatable rotating disk 12 is installed at the upper end of the bottom plate 13, the rotating disk 12 rotates with a central axis 14 at the lower end as the center, a rotating device 11 is installed at the upper end of the rotating disk 12, a torsion spring 10 is sleeved at the outer wall of the rotating device 11, a bottle body insert 9 is installed at the upper end of the rotating device 11, a plurality of rubber balls 8 are distributed at the inner position of the bottle body insert 9, a plurality of bottle body inserts 9 are provided, an antibody bottle 2, a washing buffer bottle 3, a fixative bottle 4, a permeate bottle 5 and a calibration microbead bottle 6 are respectively inserted at the inner positions of the plurality of bottle body inserts 9, and a bottle cap 7 is installed at the upper end of the antibody bottle 2, the washing buffer bottle 3, the fixative bottle 4, the permeate bottle 5 and the calibration microbead bottle 6.

[0023] First, prepare the cell sample to be tested. After the sample is pretreated, a single cell suspension is obtained, and the cells to be tested are mixed with the fluorescent labeled antibodies in the antibody bottle 2, so that the antibodies bind to specific molecules on the cell surface or inside, and the cells are labeled. The labeled cells are washed to remove unbound antibodies and impurities, and the buffer in the washing buffer bottle 3 is used to fix the cells to maintain their morphological structure. The solution in the fixative bottle 4 is used to treat the fixed cells to increase the permeability of the cell membrane. The solution in the permeation liquid bottle 5 is used to inject the treated cell sample into the flow cytometer, and the characteristics of the fluorescent markers in the cells are measured by laser. The microbeads in the calibration microbead bottle 6 are used to calibrate and calibrate the flow cytometer. Through these steps, research on cell surface markers, cell cycle, cell apoptosis, etc. can be achieved.

[0024] like Figure 2 and Figure 3 As shown, the internal position of the antibody bottle 2 stores fluorescently labeled monoclonal antibodies, the internal position of the washing buffer bottle 3 stores a buffer for washing sample cells, the internal position of the fixative bottle 4 stores a solution for fixing cells, the internal position of the permeate bottle 5 stores a solution for increasing cell membrane permeability, and the calibration microbead bottle 6 stores microbeads for flow cytometer calibration and calibration. The antibody bottle 2, the washing buffer bottle 3, the fixative bottle 4, the permeate bottle 5 and the calibration microbead bottle 6 are all inserted into the interior of the bottle body insert 9. The inner position of the bottle insert tube 9 will cause the rubber ball 8 to rotate when the reagent bottle is inserted, and the rubber ball 8 fits tightly with the reagent bottle. The bottle insert tube 9 can change its angle through the rotating device 11. The torsion spring 10 makes the bottle insert tube 9 always face the upper position after the external force disappears. The rotating disk 12 allows the bottle insert tube 9 to rotate. The rotation of the rotating disk 12 takes the central axis 14 as the center. The central axis 14 allows the rotating disk 12 to be installed with the bottom plate 13, and the bottom plate 13 is fixed at the inner position of the reagent box body 1.

[0025] There are multiple rubber balls 8 distributed inside the bottle insert 9, which will rotate when inserting or taking out, so that taking out is smoother, and the angle of the bottle insert 9 will change with the force. The angle of the bottle insert 9 can be changed by the rotating device 11, and after the external force disappears, the torsion spring 10 makes the bottle insert 9 return to the upper position.

[0026] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A detection kit based on flow cytometry, characterized in that, The invention comprises a reagent box body (1), wherein a bottom plate (13) is fixed at the upper end of the inside of the reagent box body (1), a rotating disk (12) is installed at the upper end of the bottom plate (13), the rotating disk (12) rotates around a central axis (14) at the lower end, a rotating device (11) is installed at the upper end of the rotating disk (12), a torsion spring (10) is sleeved at the outer wall of the rotating device (11), and a rotating device (11) is installed at the upper end of the rotating device (11). A bottle body insert (9) is provided, and a plurality of rubber balls (8) are distributed at the internal position of the bottle body insert (9). The bottle body insert (9) is provided with a plurality of them, and an antibody bottle (2), a washing buffer bottle (3), a fixative bottle (4), a permeate bottle (5) and a calibration microbead bottle (6) are respectively inserted at the internal positions of the plurality of bottle body inserts (9). Bottle caps (7) are installed at the upper ends of the antibody bottle (2), the washing buffer bottle (3), the fixative bottle (4), the permeate bottle (5) and the calibration microbead bottle (6).

2. A flow cytometry-based detection kit according to claim 1, characterized in that: The antibody bottle (2) contains fluorescently labeled monoclonal antibodies at an internal position, and the washing buffer bottle (3) contains a buffer for washing sample cells at an internal position.

3. A flow cytometry-based detection kit according to claim 1, characterized in that: A solution for fixing cells is stored at an internal position of the fixing solution bottle (4), and a solution for increasing the permeability of cell membranes is stored at an internal position of the permeation solution bottle (5).

4. A flow cytometry-based detection kit according to claim 1, characterized in that: The calibration microbead bottle (6) stores microbeads used for flow cytometer calibration and calibration. The antibody bottle (2), washing buffer bottle (3), fixative bottle (4), permeate bottle (5) and calibration microbead bottle (6) are all inserted into the inner position of the bottle body insert (9).

5. A flow cytometry-based detection kit according to claim 4, characterized in that: The internal position of the bottle body inserting tube (9) causes the rubber ball (8) to rotate when the reagent bottle is inserted, and the rubber ball (8) fits tightly with the reagent bottle.

6. A flow cytometry-based detection kit according to claim 5, characterized in that: The bottle insert (9) can produce angle changes through a rotating device (11), and the torsion spring (10) enables the bottle insert (9) to always face upward after the external force disappears.

7. A flow cytometry-based detection kit according to claim 6, characterized in that: The rotating disk (12) enables the bottle inserting tube (9) to rotate, and the rotation of the rotating disk (12) takes the central axis (14) as the center of the circle.

8. A flow cytometry-based detection kit according to claim 7, characterized in that: The central shaft (14) enables the rotating disk (12) to be mounted on a bottom plate (13), and the bottom plate (13) is fixed at an internal position of the reagent box body (1).

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