Living cell culture equipment

Abstract

The utility model provides living cell culture equipment, which relates to the technical field of cell culture equipment and comprises a culture cabinet, a filter component and a ventilation component, culture dishes are arranged in the middle and at the bottom of the inner wall of the culture cabinet, an oxygen concentration sensor is arranged on the inner wall, close to the culture dishes, of the culture cabinet, and the filter component is arranged on one side of the culture cabinet. The mixing assembly is arranged on the filtering assembly, the ventilation assemblies are arranged on the two sides of the culture cabinet, the filtering assembly comprises a cabinet body and a support, the cabinet body is arranged on one side of the culture cabinet, the side, away from the culture cabinet, of the cabinet body is fixedly communicated with an air inlet pipe, the support is arranged on the cabinet body, and a filter screen is arranged on the support. One air inlet pipe on the cabinet body is connected with oxygen supply equipment, the other air inlet pipe is connected with an air pump, oxygen and air are fed into the cabinet body, and when the air penetrates through the filter screen, the filter screen filters dust in the air, so that the dust is prevented from entering the culture dish to pollute living cells.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture equipment, in particular to a living cell culture equipment. Background Art

[0002] Living cell culture equipment is an instrument used to simulate the in vivo environment of a living organism in a laboratory environment to maintain and reproduce living cells. Its main function is to provide a constant temperature, humidity and gas environment so that cells can grow and reproduce in vitro.

[0003] The existing patent CN206219596U discloses a laminar flow living cell culture device. Compared with the prior art, the beneficial effects of the utility model are: the utility model has a scientific and reasonable structure, and is simple and convenient to operate. The utility model has a built-in first culture dish core, a second culture dish core and a living cell observation chamber, and can culture two different types of living cells at the same time, which is convenient for comparing data. At the same time, the living cells cultured to a certain stage can be placed inside the living cell observation chamber, which is convenient for separate observation. It is equipped with a temperature control device, a temperature sensor and a humidity sensor, which can automatically record the data of the living cell culture environment, and also facilitate the change of the temperature and humidity environmental parameters of the living cell culture, and has strong operating performance.

[0004] The above technical solution makes it easy to compare the data of the culture equipment when culturing two living cells at the same time, and change the temperature and humidity environment of cell culture through the temperature control device, thus solving the problem that the culture equipment is inconvenient to shape and change the cell culture environment.

[0005] However, the above scheme does not improve the ventilation equipment, so that the ventilation operation inside the culture dish is relatively slow, resulting in too low oxygen concentration inside the culture dish, which is not conducive to the growth of living cells. In addition, no filtering device is installed on the ventilation holes, and dust enters the interior of the culture dish, causing internal pollution. Therefore, the utility model proposes a living cell culture device. Utility Model Content

[0006] The purpose of the utility model is to solve the shortcomings of the prior art and provide a living cell culture device.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a living cell culture device, comprising a culture cabinet, a filter component, and a ventilation component, wherein the middle part and the bottom of the inner wall of the culture cabinet are both provided with a culture dish, an oxygen concentration sensor is provided on the inner wall of the culture cabinet close to the culture dish, the filter component is provided on one side of the culture cabinet, a mixing component is provided on the filter component, and the ventilation component is provided on both sides of the culture cabinet;

[0008] The filter assembly includes a cabinet and a bracket. The cabinet is arranged on one side of the culture cabinet. An air inlet pipe is fixedly connected to the side of the cabinet away from the culture cabinet. The bracket is arranged on the cabinet and a filter is arranged on the bracket.

[0009] As a preferred embodiment, a slider is fixedly connected to one side of the bracket close to the inner wall of the culture cabinet, a slide groove is provided on one side of the culture cabinet close to the slider, and the bracket is movably inserted into the culture cabinet through the slider and the slide groove.

[0010] The technical effect of adopting the above further scheme is: the bracket is inserted into the cabinet along the slide groove, which is convenient for disassembling and assembling the bracket, and convenient for removing the air intake pipe from the inside of the cabinet, thereby facilitating cleaning of the air intake pipe and avoiding excessive dust accumulation thereon, which may cause blockage.

[0011] As a preferred embodiment, as shown in the figure, the ventilation assembly includes a ventilation duct and a baffle, the ventilation duct is fixedly connected to the two sides of the incubator, a fan is arranged at one end of the inner side of the ventilation duct, an electric telescopic rod is fixedly connected to the end of the inner side of the ventilation duct away from the fan, the baffle is fixedly connected to the output end of the electric telescopic rod, and an activated carbon filter layer is arranged at the end of the inner wall of the ventilation duct close to the fan.

[0012] The technical effect of adopting the above-mentioned further scheme is: starting the electric telescopic rod, so that the electric telescopic rod pushes the baffle out from the inside of the ventilation duct, thereby connecting the ventilation duct with the inside of the cabinet, and then starting the fan, the fan sucks the air inside the cabinet into the interior of the incubator, thereby increasing the oxygen content inside the incubator, and then starting the electric telescopic rod and the fan on the other side, so that they discharge the exhaust gas inside the incubator, thereby balancing the oxygen concentration of the air inside the incubator, and when the air passes through the activated carbon filter layer, the activated carbon filter layer filters the bacteria in the air to prevent the living cells inside the culture dish from being contaminated, and when the electric telescopic rod contracts, the baffle closes the ventilation duct, disconnects the ventilation duct from the cabinet, thereby preventing the air inside the incubator from flowing back to the interior of the cabinet.

[0013] As a preferred implementation, a humidifier is provided on the cabinet body, and a humidity sensor is provided on a side of the inner wall of the culture cabinet close to the oxygen concentration sensor.

[0014] The technical effect of adopting the above further scheme is: the humidity sensor detects the humidity inside the incubator. If the humidity is too low, the humidifier is started to humidify the air inside the cabinet, and then the air enters the incubator through the ventilation pipe, thereby adjusting the humidity inside the incubator.

[0015] As a preferred embodiment, the mixing assembly includes a motor and fan blades, the motor is fixedly connected to the top of the cabinet, the output end of the motor is fixedly connected to a rotating shaft, the rotating shaft is rotatably connected to the cabinet, and the fan blades are arranged on the rotating shaft.

[0016] The technical effect of adopting the above further solution is: starting the motor, the motor drives the fan blades to rotate through the rotating shaft and the connecting block, thereby fully mixing the water mist and the air.

[0017] As a preferred embodiment, a connecting block is fixedly connected to one side of the fan blade close to the rotating shaft, and a bolt is provided on the connecting block. The fan blade is fixedly connected to the rotating shaft through the connecting block and the bolt, and an anti-slip coating is provided on one side of the connecting block close to the rotating shaft.

[0018] The technical effect of adopting the above further scheme is: bolts are installed on the connecting block. When the bolts are tightened, the connecting block is moved toward the direction of the rotating shaft by the force, and then fixed on the rotating shaft, and an anti-slip coating is applied on the connecting block to prevent the connecting block from slipping on the rotating shaft.

[0019] Compared with the prior art, the advantages and positive effects of the utility model are:

[0020] 1. By setting up a filter component, connect the air intake pipe on the cabinet to the oxygen supply equipment, connect another air intake pipe to the air pump, and send oxygen and air into the cabinet. When the air passes through the filter, the filter will filter the dust in the air to prevent the dust from entering the interior of the culture dish and contaminating the living cells. Pull the bracket to move it along the slide groove through the slider, and pull the bracket out of the cabinet, so as to facilitate the cleaning of the air intake pipe and avoid excessive dust accumulation on it, which will cause blockage.

[0021] 2. By setting up the ventilation component and starting the electric telescopic rod, the electric telescopic rod extends to push the baffle out from the inside of the ventilation duct, so that the ventilation duct is connected with the cabinet, and the fan is started to send the air in the cabinet into the interior of the incubator. Then, the fan and the electric telescopic rod on the other side are started to connect the incubator with the outside and discharge the exhaust gas inside, thereby balancing the oxygen concentration of the air inside the incubator to avoid the oxygen concentration inside the incubator being too low, thereby preventing the growth rate of cells in a low oxygen environment from slowing down. When the ventilation duct is closed, the air inside the incubator is prevented from flowing back to the interior of the cabinet. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A schematic diagram of the structure of a living cell culture device provided by the utility model;

[0023] Figure 2 This is a schematic diagram of the structure of a filtration component in a living cell culture device provided by the utility model;

[0024] Figure 3 In a living cell culture device provided by the utility model Figure 2 Enlarged view of point A above;

[0025] Figure 4 A side cross-sectional view of a ventilation tube in a living cell culture device provided by the utility model;

[0026] Figure 5 This is a disassembled diagram of a connecting block in a living cell culture device provided by the utility model.

[0027] Legend:

[0028] 1. Culture cabinet; 2. Culture dish; 3. Oxygen concentration sensor;

[0029] 4. Filter assembly; 41. Cabinet; 42. Air inlet pipe; 43. Bracket; 44. Filter screen; 45. Sliding block; 46. Sliding groove;

[0030] 5. Ventilation assembly; 51. Ventilation pipe; 52. Fan; 53. Electric telescopic rod; 54. Baffle; 55. Activated carbon filter layer;

[0031] 6. Mixing assembly; 61. Motor; 62. Rotating shaft; 63. Fan blades; 64. Connecting block; 65. Anti-slip coating;

[0032] 7. Humidifier; 8. Humidity sensor. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0034] like Figure 1-Figure 5 As shown, this embodiment provides a technical solution: a living cell culture device, comprising a culture cabinet 1, a filter component 4, and a ventilation component 5, wherein a culture dish 2 is arranged at the middle and bottom of the inner wall of the culture cabinet 1, an oxygen concentration sensor 3 is arranged on the inner wall of the culture cabinet 1 close to the culture dish 2, the filter component 4 is arranged on one side of the culture cabinet 1, a mixing component 6 is arranged on the filter component 4, and the ventilation components 5 are arranged on both sides of the culture cabinet 1;

[0035] like Figure 2-Figure 3As shown, the filter assembly 4 includes a cabinet 41 and a bracket 43. The cabinet 41 is arranged on one side of the culture cabinet 1. An air inlet pipe 42 is fixedly connected to the side of the cabinet 41 away from the culture cabinet 1. The bracket 43 is arranged on the cabinet 41. A filter screen 44 is arranged on the bracket 43. By installing the oxygen concentration sensor 3 inside the culture cabinet 1, the oxygen concentration in the air inside the culture cabinet 1 can be judged by the oxygen concentration sensor 3. The upper air inlet pipe 42 is connected to the external supply equipment to allow oxygen to enter the cabinet 41. The air inlet pipe 42 near the lower side of the cabinet 41 is connected to the external air pump to allow air to enter the culture cabinet 1. In this process, the filter screen 44 inside the cabinet 41 filters dust that may be carried in the air to prevent dust from entering the interior of the culture cabinet 1, thereby solving the problem of dust entering the interior of the culture dish 2 and causing internal pollution. In addition, by introducing air into the cabinet 41 and mixing it with oxygen, the oxygen content inside can be adjusted to prevent the oxygen concentration entering the culture dish 2 from being too high.

[0036] Furthermore, a slider 45 is fixedly connected to one side of the bracket 43 close to the inner wall of the culture cabinet 1, and a slide groove 46 is opened on one side of the culture cabinet 1 close to the slider 45. The bracket 43 is movably inserted into the culture cabinet 1 through the slider 45 and the slide groove 46. By inserting the bracket 43 into the cabinet body 41 along the slide groove 46, it is convenient to disassemble and assemble the bracket 43, and it is convenient to remove the air inlet pipe 42 from the inside of the cabinet body 41, thereby facilitating the cleaning of the air inlet pipe 42 to avoid excessive dust accumulation thereon and causing blockage.

[0037] In the above solution, there is still a problem that the air density inside the culture cabinet 1 is greater than the air density in the cabinet 41, which causes the air inside the culture cabinet 1 to flow back into the cabinet 41. Figure 4As shown, the ventilation assembly 5 includes a ventilation pipe 51 and a baffle 54. The ventilation pipe 51 is fixedly connected to both sides of the culture cabinet 1. A fan 52 is arranged at one end of the inner side of the ventilation pipe 51. An electric telescopic rod 53 is fixedly connected to the end of the inner side of the ventilation pipe 51 away from the fan 52. The baffle 54 is fixedly connected to the output end of the electric telescopic rod 53. An activated carbon filter layer 55 is arranged at one end of the inner wall of the ventilation pipe 51 close to the fan 52. By starting the electric telescopic rod 53, the electric telescopic rod 53 pushes the baffle 54 out of the interior of the ventilation pipe 51, thereby connecting the ventilation pipe 51 with the interior of the cabinet 41. Then, the fan 52 is started, and the fan 52 is blown away. The air inside the cabinet 41 is sucked into the interior of the culture cabinet 1, thereby increasing the oxygen content inside the culture cabinet 1, and then the electric telescopic rod 53 and the fan 52 on the other side are started to discharge the exhaust gas inside the culture cabinet 1, thereby balancing the oxygen concentration of the air inside the culture cabinet 1, and when the air passes through the activated carbon filter layer 55, the activated carbon filter layer 55 filters the bacteria in the air to prevent the living cells inside the culture dish 2 from being contaminated. When the electric telescopic rod 53 contracts and the baffle 54 closes the ventilation pipe 51, the ventilation pipe 51 is disconnected from the cabinet 41, thereby preventing the air inside the culture cabinet 1 from flowing back to the interior of the cabinet 41.

[0038] Furthermore, a humidifier 7 is provided on the cabinet body 41, and a humidity sensor 8 is provided on one side of the inner wall of the incubator 1 close to the oxygen concentration sensor 3. The humidity inside the incubator 1 is detected by the humidity sensor 8. If the humidity is too low, the humidifier 7 is started to humidify the air inside the cabinet body 41, and then the air enters the interior of the incubator 1 through the ventilation pipe 51, thereby adjusting the humidity inside the incubator 1.

[0039] In the above scheme, there is still a situation that the water mist sprayed by the humidifier 7 cannot be fully mixed with the air, such as Figure 2 and Figure 5 As shown, the mixing component 6 includes a motor 61 and a fan blade 63. The motor 61 is fixedly connected to the top of the cabinet 41. The output end of the motor 61 is fixedly connected to a rotating shaft 62. The rotating shaft 62 is rotatably connected to the cabinet 41. The fan blade 63 is arranged on the rotating shaft 62. By starting the motor 61, the motor 61 drives the fan blade 63 to rotate through the rotating shaft 62 and the connecting block 64, thereby fully mixing the water mist and the air.

[0040] A connecting block 64 is fixedly connected to one side of the fan blade 63 close to the rotating shaft 62, and a bolt is provided on the connecting block 64. The fan blade 63 is fixedly connected to the rotating shaft 62 through the connecting block 64 and the bolt. An anti-slip coating 65 is provided on one side of the connecting block 64 close to the rotating shaft 62. By installing the bolt on the connecting block 64 and tightening the bolt, the connecting block 64 is subjected to the force to move toward the direction of the rotating shaft 62, and is then fixed on the rotating shaft 62. The connecting block 64 is coated with the anti-slip coating 65 to prevent the connecting block 64 from slipping on the rotating shaft 62.

[0041] Working principle: Figure 1-Figure 5 As shown:

[0042] During the cell culture process: first, living cells are placed inside the culture dish 2, and then the culture dish 2 is placed inside the culture cabinet 1, the culture cabinet 1 is closed, and the culture equipment is used to culture living cells, and then the oxygen concentration sensor 3 and the humidity sensor 8 are started to detect the oxygen concentration and humidity inside the culture cabinet 1 respectively.

[0043] When the oxygen concentration is too low, the air inlet pipe 42 near the top of the cabinet 41 is first connected to the oxygen supply equipment to allow oxygen to enter the interior of the cabinet 41, and then the air inlet pipe 42 near the bottom of the cabinet 41 is connected to the air pump to send air into the interior of the cabinet 41, thereby adjusting the oxygen concentration in the air. When the air passes through the filter 44, the filter 44 filters the dust in the air to prevent the dust from entering the interior of the culture dish 2 and contaminating the living cells. Then, the electric telescopic rod 53 is started to push the baffle 54 out of the ventilation pipe 51 to connect the ventilation pipe 51 with the cabinet 41, and then the fan 52 is started to suck the air inside the cabinet 41 into the interior of the culture cabinet 1, thereby increasing the oxygen concentration inside the culture cabinet 1. When the air passes through the activated carbon filter layer 55, the activated carbon filter layer 55 filters the bacteria in the air to prevent the living cells inside the culture dish 2 from being contaminated. At the same time, the electric telescopic rod 53 away from the cabinet 41 is started to connect the culture cabinet 1 with the outside and discharge the exhaust gas inside the culture cabinet 1.

[0044] When the humidity is too low, start the humidifier 7 to spray water mist on the interior of the cabinet 41, and then start the motor 61 to make the shaft 62 drive the fan blades 63 to rotate through the connecting block 64, so that the water mist and air are fully mixed, and then the humid air is sent into the interior of the culture cabinet 1 to maintain the humidity inside.

[0045] When the culture equipment needs maintenance after long-term use, the bolts on the connecting block 64 are removed to separate the connecting block 64, so as to facilitate the inspection of whether the fan blade 63 is damaged. At the same time, the bracket 43 is pulled, and the gravel bracket 43 slides along the slide groove 46 through the slider 45, so that the bracket 43 can be easily pulled out to facilitate cleaning of the filter 44.

[0046] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A living cell culture device, comprising a culture cabinet (1), a filter component (4), and a ventilation component (5), characterized in that: A culture dish (2) is arranged at the middle and bottom of the inner wall of the culture cabinet (1); an oxygen concentration sensor (3) is arranged on the inner wall of the culture cabinet (1) close to the culture dish (2); the filter component (4) is arranged on one side of the culture cabinet (1); a mixing component (6) is arranged on the filter component (4); and the ventilation component (5) is arranged on both sides of the culture cabinet (1); The filter assembly (4) comprises a cabinet (41) and a bracket (43); the cabinet (41) is arranged on one side of the culture cabinet (1); an air inlet pipe (42) is fixedly connected to the side of the cabinet (41) away from the culture cabinet (1); the bracket (43) is arranged on the cabinet (41); and a filter screen (44) is arranged on the bracket (43).

2. A living cell culture device according to claim 1, characterized in that: A slider (45) is fixedly connected to one side of the support (43) close to the inner wall of the culture cabinet (1); a slide groove (46) is provided on one side of the culture cabinet (1) close to the slide groove (45); and the support (43) is movably inserted into the culture cabinet (1) via the slide groove (45) and the slide groove (46).

3. A living cell culture device according to claim 1, characterized in that: The ventilation assembly (5) comprises a ventilation pipe (51) and a baffle (54); the ventilation pipe (51) is fixedly connected to two sides of the culture cabinet (1); a fan (52) is arranged at one end of the inner side of the ventilation pipe (51); an electric telescopic rod (53) is fixedly connected to the end of the inner side of the ventilation pipe (51) away from the fan (52); the baffle (54) is fixedly connected to the output end of the electric telescopic rod (53); and an activated carbon filter layer (55) is arranged at one end of the inner wall of the ventilation pipe (51) close to the fan (52).

4. A living cell culture device according to claim 1, characterized in that: A humidifier (7) is provided on the cabinet body (41), and a humidity sensor (8) is provided on a side of the inner wall of the culture cabinet (1) close to the oxygen concentration sensor (3).

5. A living cell culture device according to claim 1, characterized in that: The mixing assembly (6) comprises a motor (61) and a fan blade (63); the motor (61) is fixedly connected to the top of the cabinet (41); the output end of the motor (61) is fixedly connected to a rotating shaft (62); the rotating shaft (62) is rotatably connected to the cabinet (41); and the fan blade (63) is arranged on the rotating shaft (62).

6. A living cell culture device according to claim 5, characterized in that: A connecting block (64) is fixedly connected to one side of the fan blade (63) close to the rotating shaft (62), and a bolt is arranged on the connecting block (64). The fan blade (63) is fixedly connected to the rotating shaft (62) through the connecting block (64) and the bolt, and an anti-slip coating (65) is arranged on one side of the connecting block (64) close to the rotating shaft (62).

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