Brain slice transfer rod
By designing a brain sheet transfer rod including a hand handle and an operating rod, the bent section is used to pick up the brain sheet, which solves the problems of brush clamping and liquid dilution, and improves the transfer efficiency and dyeing consistency.
Patent Information
- Application Number
- CN202421315628.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-11
- Publication Date
- 2025-02-25
- Estimated Expiration
- 2034-06-11
AI Technical Summary
Existing brushes tend to clamp brain tablets during brain tablet transfer, increasing the risk of damage, and may lead to liquid dilution, affecting the staining effect, and reducing experimental efficiency and consistency.
A brain piece transfer rod is designed, including a hand handle and an operating rod. The distal end of the operating rod is provided with a bent section. The bent section can provoke the brain piece, reduce mechanical damage, and design through specific angles and round heads. Avoid cross-contamination and dilution of liquids.
It significantly improves the efficiency and accuracy of brain tablet metastasis, reduces mechanical damage and liquid dilution problems of brain tablets, and ensures the consistency and quality of the staining effect.
Smart Images
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Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of medical equipment, and specifically relates to a brain slice transfer rod. Background Art
[0002] Brain slice experiments use staining technology to mark specific proteins on brain slices in order to accurately detect their expression levels or expression areas, thereby supporting neuroscience research and disease diagnosis. During the process, the cut brain slices need to be placed one by one in the wells containing blocking fluid, and after shaking on a shaker, they need to be transferred for subsequent operations.
[0003] In the process of transferring brain slices, a writing brush is usually used as the main tool, which has some significant disadvantages; the structure of the writing brush is composed of many fine hairs, which easily form gaps between the hairs when immersed in liquid, causing the brain slice to be clamped by the writing brush during transfer, which not only increases the risk of brain slice damage, but also makes it difficult for the brain slice to fall off smoothly into the rinsing solution; the writing brush will inevitably carry some liquid when transferring brain slices. When transferring from the rinsing solution to a smaller volume of dye solution, these residual liquids may cause the dye solution to be diluted, thereby affecting the dyeing effect.
[0004] Using a brush as a brain slice transfer tool has low experimental efficiency and increases the complexity and potential error rate of the operation.
[0005] In view of the above problems, the utility model provides a brain slice transfer rod. Utility Model Content
[0006] In order to overcome the problems raised in the background technology, the design of the brain slice transfer rod is improved, which can effectively avoid the dilution of the dye solution and affect the dyeing effect, improve the consistency and accuracy of the dyeing, and also improve the work efficiency of the experimenters. The utility model adopts the following technical solutions:
[0007] A brain slice transfer rod comprises a handheld handle and an operating rod; the handheld handle has a first end and a second end, the first end of the handheld handle is fixedly connected to the operating rod, the end of the operating rod away from the handheld handle is provided with a bending section, the bending section is fixedly connected to the operating rod, and the angle between the bending section and the operating rod is an obtuse angle; the bending section can lift the brain slice, so that the experimenter can transfer the brain slice to the rinsing solution more quickly, which significantly improves the work efficiency, accurately controls and optimizes the contact area of the brain slice, greatly reduces the mechanical damage to the brain slice, and reduces the influence of the brain slice on the liquid concentration when transferring between different liquids, especially when transferring the brain slice from the rinsing solution to the dye solution, effectively avoiding the liquid cross contamination and dilution problems.
[0008] Furthermore, the angle between the bending section and the operating rod is 120 degrees. This specific angle layout makes it more convenient and efficient for experimenters to operate the brain slice selection and transfer process.
[0009] Furthermore, a round head is provided at the end of the bending section away from the operating rod, which can ensure that no damage is caused to the brain slice when the brain slice is supported and transferred.
[0010] Furthermore, the outer surfaces of the operating rod and the bending section are set to be smooth and made of hard material.
[0011] Furthermore, the shape of the hand-held handle is cylindrical, and the diameter of the first end of the hand-held handle gradually decreases. The above arrangement is conducive to observation by experimenters and prevents the first end of the hand-held handle from being too thick and blocking the line of sight.
[0012] Furthermore, the axial direction of the operating rod is perpendicular to the end surface of the first end of the hand-held handle.
[0013] Furthermore, the hand-held handle is provided with anti-slip grooves, which ensures a stable grip when used by an experimenter and prevents slipping during use.
[0014] Furthermore, the diameter of the hand-held handle is 6 mm. The above configuration provides a stable and comfortable grip for the experimenter, thereby enhancing control accuracy and reducing fatigue.
[0015] Furthermore, the diameter of the operating rod and the bending section is 1 mm, which can avoid dilution of the liquid while ensuring that the brain slice will not be damaged due to being too sharp.
[0016] The beneficial effects of the utility model are as follows: the utility model has a reasonable structure; by providing an operating rod and a bending section, the bending section can lift the brain slice, and the experimenter can transfer the brain slice to the rinsing solution more quickly, which significantly improves work efficiency, accurately controls and optimizes the contact area of the brain slice, greatly reduces mechanical damage to the brain slice, and reduces the influence of the brain slice on the liquid concentration when transferring between different liquids, especially when transferring the brain slice from the rinsing solution to the dye solution, effectively avoids liquid cross-contamination and dilution problems; by providing anti-slip patterns on the handheld handle, it ensures that the experimenter has a stable grip when using it, prevents sliding during use, and can significantly improve the accuracy of experimental operations and the user experience. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0018] Figure 2 This is a schematic diagram of the structure of the handheld handle of the utility model;
[0019] Figure 3 It is a schematic diagram of the structure of the cooperation between the operating rod and the bending section of the utility model;
[0020] Figure 4 This is a schematic diagram of the overall structure of the utility model when anti-slip patterns are provided on the hand-held handle;
[0021] Figure 5 It is a schematic diagram of the overall structure when anti-slip glue dots are arranged on the hand-held handle of the utility model;
[0022] Figure 6 This is a schematic diagram of the overall structure of the utility model when an anti-slip rubber strip is provided on the hand-held handle;
[0023] In the figure, 1. Handle; 2. Operating lever; 3. Bending section; 4. Round head; 5. Anti-slip pattern; 6. Anti-slip glue dots; 7. Anti-slip rubber strip. DETAILED DESCRIPTION
[0024] The technical solutions in the embodiments of the utility model are clearly and completely described below through specific embodiments. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Those skilled in the art can easily understand other advantages and functions of the utility model from the contents disclosed in this specification. The utility model can also be implemented or applied through other different specific implementation methods. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0025] Example
[0026] Please refer to Figure 1-6 In the present embodiment, a brain slice transfer rod includes a handheld handle 1 and an operating rod 2; the handheld handle 1 has a first end and a second end, the first end of the handheld handle 1 is fixedly connected to the operating rod 2, and the end of the operating rod 2 away from the handheld handle 1 is provided with a bending section 3, the bending section 3 is fixedly connected to the operating rod 2, and the angle between the bending section 3 and the operating rod 2 is an obtuse angle; the bending section 3 can pick up the brain slice, and the experimenter can transfer the brain slice to the rinsing solution more quickly, which significantly improves the work efficiency, accurately controls and optimizes the contact area of the brain slice, greatly reduces the mechanical damage to the brain slice, and reduces the influence of the brain slice on the liquid concentration when transferring between different liquids, especially when transferring the brain slice from the rinsing solution to the dye solution, effectively avoiding the problem of liquid cross contamination and dilution. During use, the brain slice is taken out for transfer through the bending section 3, and the brain slice can adhere to the bending section 3, which is convenient to use.
[0027] The connection between the bending section 3 and the operating rod 2 is smoothly connected.
[0028] Please refer to Figure 3 In this embodiment, the angle between the bending section 3 and the operating rod 2 is 100-150 degrees, preferably 120 degrees. This specific angle layout makes it more convenient and efficient for experimenters to operate brain slice selection and transfer.
[0029] Please refer to Figure 3 In this embodiment, a round head 4 is provided at the end of the bending section 3 away from the operating rod 2 to ensure that no damage is caused to the brain slice when the brain slice is supported and transferred.
[0030] The outer surfaces of the operating rod 2 and the bending section 3 are set to be smooth and made of hard material. Preferably, the material is alloy.
[0031] Please refer to Figure 2 The shape of the hand-held handle 1 is cylindrical, and the diameter at the first end of the hand-held handle 1 gradually decreases. The above arrangement is conducive to observation by experimenters and prevents the first end of the hand-held handle 1 from being too thick and blocking the line of sight.
[0032] The axial direction of the operating rod 2 is perpendicular to the end surface of the first end of the handle 1 .
[0033] Please refer to Figure 4 In this embodiment, the hand-held handle 1 is provided with anti-skid patterns 5, which ensures a stable grip when used by the experimenter and prevents slipping during use. The anti-skid patterns 5 are arranged at intervals on the hand-held handle 1 to increase the friction during use and ensure the smooth progress of the experiment.
[0034] Please refer to Figure 5 In another embodiment, an anti-skid glue dot 6 is provided on the hand-held handle 1, and the anti-skid glue dot 6 covers the hand-held handle 1. The anti-skid glue dot 6 is a soft anti-skid material. Preferably, the material of the anti-skid glue dot 6 is rubber, which can increase the friction during use.
[0035] Please refer to Figure 6 In another embodiment, an anti-skid rubber strip 7 is provided on the hand-held handle 1, and the anti-skid rubber strip 7 covers the hand-held handle 1. The anti-skid rubber point 6 is a soft anti-skid material, and preferably the material of the anti-skid rubber point 6 is rubber.
[0036] In this embodiment, the diameter of the hand-held handle 1 is 6 mm. The above setting provides the experimenter with a stable and comfortable grip, thereby enhancing the control accuracy and reducing fatigue; the diameter of the operating rod 2 and the bending section 3 is 1 mm, which can avoid liquid dilution while ensuring that the brain slice will not be damaged due to being too sharp.
[0037] Experimental operation process:
[0038] 1. First, add the blocking solution to the 24-well cell culture dish, adding 400-500 μL to each well. The blocking solution is used to prevent non-specific binding and ensure the specificity of staining.
[0039] 2. Use a brain slice transfer rod to place the slices into the wells containing the blocking solution one by one, 3-4 slices per well. Shake at 50 rpm for 1.5-2 hours on a shaker in a dark environment to promote the effect of the blocking solution.
[0040] 3. Dispense the primary antibody solution into a new 24-well cell culture dish, adding 400-500 μL to each well.
[0041] 4. Use a brain slice transfer rod to transfer the brain slices from the blocking solution to the wells containing the primary antibody solution. Place the culture dish on a shaker at 4°C away from light, set the speed to 50 rpm, and incubate overnight to allow the antibody to bind to the target protein.
[0042] 5. Place the rinse solution in a 6-well cell culture dish, about 5 ml per well, to wash the brain slices to remove unbound primary antibody.
[0043] 6. Use a brain slice transfer rod to transfer the brain slices to the wells containing the rinse solution and wash them on a light-proof shaker at 120 rpm for 5 minutes to remove unbound primary antibodies.
[0044] 7. Repeat step 6 twice to ensure thorough cleaning.
[0045] 8. Add the secondary antibody solution to a new 24-well cell culture dish, 400-500 μL per well.
[0046] 9. Use a brain slice transfer rod to transfer the brain slices to the wells containing the secondary antibody solution and place them on a shaker at 50 rpm for incubation for 2-4 hours away from light.
[0047] 10. Use the brain slice transfer rod again to transfer the brain slice to a 6-well cell culture dish containing the rinse solution, and wash it at 120 rpm for 5 minutes on a light-proof shaker.
[0048] 11. Repeat step 10 twice to ensure thorough cleaning.
[0049] 12. At this point, the brain slices are ready for patching and subsequent observation and analysis of the expression areas and levels of specific proteins.
[0050] The brain slice transfer rod ensures the safe and precise transfer of brain slices in each processing step, avoids brain slice damage and sample contamination, and improves staining quality and consistency.
[0051] The above-mentioned embodiments are only used to understand the present invention. It should be noted that, for those skilled in the art, several improvements can be made to the present invention without departing from the principle of the present invention, and these improvements will also fall within the scope of protection of the claims of the present invention.
Claims
1. A brain slice transfer rod, characterized in that: It includes a hand-held handle and an operating rod; the hand-held handle has a first end and a second end, the first end of the hand-held handle is fixedly connected to the operating rod, the end of the operating rod away from the hand-held handle is provided with a bending section, the bending section is fixedly connected to the operating rod, and the angle between the bending section and the operating rod is an obtuse angle.
2. The brain slice transfer rod according to claim 1, characterized in that: The angle between the bending section and the operating rod is 120 degrees.
3. The brain slice transfer rod according to claim 1, characterized in that: The connection between the bending section and the operating rod is smoothly connected.
4. The brain slice transfer rod according to claim 1, characterized in that: A round head is arranged at the end of the bending section away from the operating rod.
5. The brain slice transfer rod according to claim 4, characterized in that: The outer surfaces of the operating rod and the bending section are set to be smooth and made of hard material.
6. The brain slice transfer rod according to claim 1, characterized in that: The handle is cylindrical in shape, and the diameter of the handle gradually decreases at the first end.
7. The brain slice transfer rod according to claim 1, characterized in that: The axis direction of the operating rod is perpendicular to the end surface of the first end of the hand-held handle.
8. The brain slice transfer rod according to claim 1, characterized in that: The hand-held handle is provided with anti-slip patterns.
9. The brain slice transfer rod according to claim 1, characterized in that: The diameter of the hand-held handle is 6 mm.
10. The brain slice transfer rod according to any one of claims 1 to 9, characterized in that: The diameter of the operating rod and the bending section is 1 mm.