Efficient RNA (Ribonucleic Acid) extraction tank

Abstract

The utility model provides an efficient RNA (Ribonucleic Acid) extracting tank which comprises a control frame and an extracting tank arranged on a central platform of the control frame, an oblique insertion type axial stirrer is arranged on the side wall of the extracting tank, a control system is arranged on one side of the control frame, the top of the control frame is connected with a vertical stirrer, and the vertical stirrer extends into an inner cavity of the extracting tank through a top plate of the extracting tank. A hydrochloric acid fed-batch pipeline is arranged on one side of the vertical stirrer, an electronic valve is arranged at the water inlet end of the hydrochloric acid fed-batch pipeline and connected with a control system, the water outlet end of the hydrochloric acid fed-batch pipeline extends into the extraction tank through a top plate of the extraction tank, and a cold water flow adding pipe is arranged on one side of a tank body of the extraction tank. A pH value monitor and a temperature sensor are arranged on the inner side wall of the circular truncated cone-shaped tank top of the extraction tank. According to the device disclosed by the utility model, the RNA extraction efficiency is integrally improved, the safety risk of adding hydrochloric acid by production personnel is reduced, and the workload of the production personnel is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experimental equipment, in particular to a high-efficiency RNA extraction tank. Background Art

[0002] Extracting high-purity and complete RNA is helpful for further deep processing in yeast production. The existing RNA extraction storage tank has simple functions and is only equipped with a simple oblique axial stirrer with weak stirring performance. The mixing effect in the production process is poor. The pH adjustment in the production process requires manual addition of hydrochloric acid, which will cause certain personal threats to production personnel during the production process. The clear liquid cannot be transferred out in time after sedimentation, which prolongs the production time and reduces the overall production efficiency.

[0003] Therefore, there is an urgent need for an efficient RNA extraction tank to solve the above problems. Summary of the invention

[0004] The purpose of the utility model is to overcome the above-mentioned shortcomings and provide a high-efficiency RNA extraction tank to solve the problems raised in the background technology.

[0005] The utility model solves the above technical problems, and the technical scheme adopted is: an efficient RNA extraction tank, comprising a control frame and an extraction tank arranged on the central platform thereof, the side wall of the extraction tank is provided with an oblique insertion axial stirring device, one side of the control frame is a control system, the top of the control frame is connected to a vertical stirrer, the vertical stirrer extends into the inner cavity of the extraction tank through a top plate of the extraction tank, one side of the vertical stirrer is provided with a hydrochloric acid flow-adding pipeline, the water inlet end of the hydrochloric acid flow-adding pipeline is provided with an electronic valve, the electronic valve is connected to the control system, the water outlet end of the hydrochloric acid flow-adding pipeline extends into the tank through the top plate of the extraction tank, one side of the extraction tank body is provided with a cold water flow adding pipe, a hot water flow adding pipe and a clear liquid automatic transfer pipeline, and the inner side wall of the frustum-shaped tank top of the extraction tank is provided with a pH value monitor and a temperature sensor.

[0006] Preferably, a control panel is provided on the outer surface of the control system, and a display screen is provided above the control panel.

[0007] Preferably, a motor is provided on the top of the vertical agitator, and the motor is arranged inside the top of the control frame. The input end of the motor is connected to the control system through the internal circuit of the control frame. The stirring rod of the vertical agitator is provided with a double-layer stirring blade.

[0008] Preferably, the bottom of the extraction tank is set to be funnel-shaped, a camera is provided above the inner wall of the extraction tank, the camera is electrically connected to the control system through the internal circuit of the control frame, a sediment discharge outlet is provided at the center of the bottom of the extraction tank, an inlet for feeding raw materials is provided on the side of the truncated cone top of the extraction tank, and the junction of the truncated cone top of the extraction tank and the cylindrical tank body is the set liquid level line.

[0009] Preferably, the outlet end of the hydrochloric acid flow addition pipeline extends below the set liquid level line, the cold water flow addition pipe and the hot water flow addition pipe are installed above the set liquid level line, and the clear liquid automatic transfer pipeline is installed above the bottom of the extraction tank funnel.

[0010] Preferably, a vertical ladder is provided on one side of the central platform of the control frame, and a corridor for cold water supply pipes, hot water supply pipes and clear liquid automatic transfer pipelines to pass through is provided on the side adjacent to the vertical ladder of the control frame, and an inspection port is provided on the side wall of the central platform of the control frame.

[0011] Preferably, the cold water flow adding pipes and the hot water flow adding pipes inside the corridor are respectively provided with cold water electronic valves and hot water electronic valves, and the cold water electronic valves and hot water electronic valves are both connected to the control system.

[0012] Preferably, a spherical flow monitoring probe is provided at the water outlet of the automatic clear liquid transfer pipeline, and a clear liquid flow control valve is provided at the place where the automatic clear liquid transfer pipeline passes through the corridor, and the clear liquid flow control valve is connected to the control system.

[0013] Preferably, the pH value monitor and the temperature sensor are arranged on the inner side wall of the truncated cone tank top, and the sensing probes of the pH value monitor and the temperature sensor are both extended below the set liquid level line.

[0014] The utility model has the following beneficial effects:

[0015] The utility model adds the functions of automatic flow addition of cold water, hot water and hydrochloric acid to the existing extraction tank, adds the function of automatic transfer out of clear liquid, adds a vertical stirrer to improve the mixing efficiency, improves the efficiency of RNA extraction as a whole, reduces the safety risk of production personnel adding hydrochloric acid, and alleviates the workload of production personnel. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the cross-sectional structure of the utility model;

[0018] Figure 3 This is a schematic diagram of the cold and hot water flow pipe structure of the utility model;

[0019] Figure 4 This is a detailed structural diagram of the automatic liquid transfer pipeline of the utility model;

[0020] Figure 5 This is a detailed structural diagram of the vertical agitator of the utility model. DETAILED DESCRIPTION

[0021] The utility model is further described below in conjunction with the accompanying drawings and embodiments:

[0022] See also Figures 1 to 5 A high-efficiency RNA extraction tank comprises a control frame 1 and an extraction tank 2 arranged on its central platform, wherein the side wall of the extraction tank 2 is provided with an oblique axial stirring 3, one side of the control frame 1 is a control system 1.1, the top of the control frame 1 is connected to a vertical stirrer 4, the vertical stirrer 4 extends into the inner cavity of the extraction tank 2 through the top plate of the extraction tank 2, a hydrochloric acid flow-adding pipeline 5 is provided on one side of the vertical stirrer 4, the water inlet end of the hydrochloric acid flow-adding pipeline 5 is provided with an electronic valve 5.1, the electronic valve 5.1 is connected to the control system 1.1, the water outlet end of the hydrochloric acid flow-adding pipeline 5 extends into the tank through the top plate of the extraction tank 2, a cold water flow adding pipe 6, a hot water flow adding pipe 7 and a clear liquid automatic transfer pipeline 8 are provided on one side of the extraction tank body, and a pH value monitor 9 and a temperature sensor 10 are provided on the inner side wall of the truncated cone-shaped tank top of the extraction tank 2. The utility model adds the functions of automatic flow addition of cold water, hot water and hydrochloric acid to the existing extraction tank, adds the function of automatic transfer out of clear liquid, adds a vertical stirrer to improve the mixing efficiency, improves the efficiency of RNA extraction as a whole, reduces the safety risk of production personnel adding hydrochloric acid, and alleviates the workload of production personnel.

[0023] Preferably, the control system 1.1 is provided with a control panel 1.1.1 on its outer surface, and a display screen 1.1.2 is provided above the control panel 1.1.1. The technician can preset the production environment in the pipe through the control panel 1.1.1, check the status in the tank through the display screen, and perform manual intervention in time according to the reaction status in the tank.

[0024] Preferably, a motor 4.1 is provided on the top of the vertical stirrer 4, and the motor 4.1 is provided inside the top of the control frame 1. The input end of the motor 4.1 is connected to the control system 1.1 through the internal circuit of the control frame 1. A double-layer stirring blade 4.2 is provided on the stirring rod of the vertical stirrer 4. The original extraction tank is only provided with an oblique insertion axial stirring 3. On this basis, the present device adds a vertical stirrer 4 to improve the mixing efficiency.

[0025] Preferably, the bottom of the extraction tank 2 is set to be funnel-shaped, a camera 2.1 is provided above the inner wall of the extraction tank 2, the camera 2.1 is electrically connected to the control system 1.1 through the internal circuit of the control frame 1, a sediment discharge port 2.2 is provided at the bottom center of the extraction tank 2, a raw material inlet 2.3 is provided on the side of the truncated cone top of the extraction tank 2, and a liquid level line 2.4 is set at the junction of the truncated cone top and the cylindrical tank body of the extraction tank 2. By providing the camera 2.1, the situation inside the tank can be displayed on a large screen in a timely manner, and the technicians can check it in time to avoid unexpected situations.

[0026] Preferably, the outlet end of the hydrochloric acid feed line 5 extends below the set liquid level line 2.4, the cold water flow water pipe 6 and the hot water flow water pipe 7 are set above the set liquid level line 2.4, and the clear liquid automatic transfer line 8 is set above the bottom of the funnel of the extraction tank 2. The outlet end of the hydrochloric acid feed line 5 extends below the set liquid level line 2.4 to directly feed hydrochloric acid into the solution, and the cold water flow water pipe 6 and the hot water flow water pipe 7 do not contact the production solution.

[0027] Preferably, a vertical ladder 1.3 is provided on one side of the central platform of the control frame 1, a corridor 1.4 is provided on the side of the control frame 1 adjacent to the vertical ladder 1.3, through which a cold water supply pipe 6, a hot water supply pipe 7 and a clear liquid automatic transfer pipeline 8 pass, and an inspection port 1.2 is provided on the side wall of the central platform of the control frame 1. The vertical ladder is provided to facilitate technicians to perform daily maintenance and overhaul on the top of the device, and the corridor 1.4 can be provided with an electronic valve on the pipeline, and the space here is convenient for connecting the electronic valve with the line of the control system.

[0028] Preferably, the cold water flow water supply pipe 6 and the hot water flow water supply pipe 7 in the corridor 1.4 are respectively provided with a cold water electronic valve 6.1 and a hot water electronic valve 7.1, and the cold water electronic valve 6.1 and the hot water electronic valve 7.1 are both connected to the control system 1.1. Placing the cold water electronic valve 6.1 and the hot water electronic valve 7.1 in the corridor 1.4 can facilitate the wiring inside the control frame, thereby enhancing the overall practicality of the device.

[0029] Preferably, a spherical flow monitoring probe 8.1 is provided at the outlet end of the clear liquid automatic transfer pipeline 8, and a clear liquid flow control valve 8.2 is provided at the place where the clear liquid automatic transfer pipeline 8 passes through the corridor 1.4, and the clear liquid flow control valve 8.2 is connected to the control system.

[0030] Preferably, the pH monitor 9 and the temperature sensor 10 are arranged on the inner side wall of the truncated cone tank top, and the sensing probes of the pH monitor 9 and the temperature sensor 10 are both extended below the set liquid level line 2.4. To avoid reaction with the production solution, the pH monitor 9 and the temperature sensor 10 are placed above the liquid level, and only the sensing probe is extended below the liquid level to extend the service life of the device.

[0031] The working principle of this embodiment is as follows:

[0032] Put the production raw materials into the tank body from the top inlet 2.3 of the extraction tank 2, start the device through the control panel 1.1.1, control the cold water flow adding pipe 6, the hot water flow adding pipe 7 to add water, and the hydrochloric acid flow adding pipeline 5 to add hydrochloric acid. The technician can observe the reaction state in the tank in real time through the display screen 1.1.2, and start the vertical agitator to stir the production liquid to accelerate the mixing effect;

[0033] During the stirring process, the temperature and pH value in the tank are fed back to the control system 1.1 in real time through the temperature sensor 10 and the pH value monitor 9 according to the temperature threshold and pH threshold set by the technician in the control system 1.1. Once the control system 1.1 detects that the pH value and temperature change exceed the set range, the control system 1.1 will issue an instruction to control the valve to adjust the water flow of hydrochloric acid and hot and cold water until the preset water level line is reached, and stop adding water and hydrochloric acid. When the solution is mixed, stop stirring and start to settle;

[0034] When the sedimentation reaches the set time, the control system 1.1 will automatically open the clear liquid flow control valve 8.2, and the clear liquid will be automatically transferred out from the clear liquid automatic transfer pipeline 8. When the spherical flow monitoring probe 8.1 detects that the clear liquid transfer is completed, it will be fed back to the control system 1.1 to close the clear liquid flow control valve 8.2, and the sediment can be discharged from the tank through the tank bottom sediment discharge port 2.2 for the next process.

[0035] The above embodiments are only preferred technical solutions of the present invention and should not be regarded as limitations of the present invention. The protection scope of the present invention shall be the technical solutions recorded in the claims, including equivalent replacement solutions of the technical features in the technical solutions recorded in the claims. That is, equivalent replacement improvements within this scope are also within the protection scope of the present invention.

Claims

1. An efficient RNA extraction tank, comprising a control frame (1) and an extraction tank (2) arranged on the central platform thereof, wherein the side wall of the extraction tank (2) is provided with an obliquely inserted axial stirring device (3), characterized in that: One side of the control frame (1) is a control system (1.1). The top of the control frame (1) is connected to a vertical agitator (4). The vertical agitator (4) extends into the inner cavity of the extraction tank (2) through the top plate of the extraction tank (2). One side of the vertical agitator (4) is provided with a hydrochloric acid feed pipeline (5). The water inlet end of the hydrochloric acid feed pipeline (5) is provided with an electronic valve (5.1). The electronic valve (5.1) is connected to the control system (1.1). The water outlet end of the hydrochloric acid feed pipeline (5) extends into the extraction tank through the top plate of the extraction tank (2). One side of the extraction tank (2) is provided with a cold water flow feed pipe (6), a hot water flow feed pipe (7) and a clear liquid automatic transfer pipe (8). The inner side wall of the truncated cone top of the extraction tank (2) is provided with a pH value monitor (9) and a temperature sensor (10).

2. The efficient RNA extraction tank according to claim 1, characterized in that: A control panel (1.1.1) is provided on the outer surface of the control system (1.1), and a display screen (1.1.2) is provided above the control panel (1.1.1).

3. The efficient RNA extraction tank according to claim 1, characterized in that: A motor (4.1) is provided at the top of the vertical agitator (4), and the motor (4.1) is arranged inside the top of the control frame (1). The input end of the motor (4.1) is connected to the control system (1.1) via an internal circuit of the control frame (1). A double-layered stirring blade (4.2) is provided on the stirring rod of the vertical agitator (4).

4. The efficient RNA extraction tank according to claim 1, characterized in that: The bottom of the extraction tank (2) is designed to be funnel-shaped. A camera (2.1) is provided above the inner wall of the extraction tank (2). The camera (2.1) is electrically connected to the control system (1.1) via internal circuits of the control frame (1). A sediment discharge port (2.2) is provided at the center of the bottom of the extraction tank (2). An inlet (2.3) for feeding raw materials is provided on the side of the truncated cone-shaped tank top of the extraction tank (2). The junction between the truncated cone-shaped tank top and the cylindrical tank body of the extraction tank (2) is a set liquid level line (2.4).

5. The efficient RNA extraction tank according to claim 1, characterized in that: The outlet end of the hydrochloric acid flow addition pipeline (5) extends below the set liquid level line (2.4), the cold water flow addition pipe (6) and the hot water flow addition pipe (7) are installed above the set liquid level line (2.4), and the clear liquid automatic transfer pipeline (8) is installed above the bottom of the funnel of the extraction tank (2).

6. The efficient RNA extraction tank according to claim 1, characterized in that: A vertical ladder (1.3) is provided on one side of the central platform of the control frame (1), a corridor (1.4) for the cold water supply pipe (6), the hot water supply pipe (7) and the clear liquid automatic transfer pipeline (8) to pass through is provided on the side of the control frame (1) adjacent to the vertical ladder (1.3), and a side wall of the central platform of the control frame (1) is provided with an inspection port (1.2).

7. The efficient RNA extraction tank according to claim 6, characterized in that: A cold water electronic valve (6.1) and a hot water electronic valve (7.1) are respectively provided on the cold water flow adding pipe (6) and the hot water flow adding pipe (7) inside the corridor (1.4), and the cold water electronic valve (6.1) and the hot water electronic valve (7.1) are both connected to the control system (1.1).

8. The efficient RNA extraction tank according to claim 1, characterized in that: A spherical flow monitoring probe (8.1) is provided at the outlet end of the clear liquid automatic transfer pipeline (8), and a clear liquid flow control valve (8.2) is provided at the location where the clear liquid automatic transfer pipeline (8) passes through the corridor (1.4), and the clear liquid flow control valve (8.2) is connected to a control system.

9. The efficient RNA extraction tank according to claim 1, characterized in that: The pH value monitor (9) and the temperature sensor (10) are arranged on the inner side wall of the truncated cone tank top, and the sensing probes of the pH value monitor (9) and the temperature sensor (10) are both extended below the set liquid level line (2.4).

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