One-step fluorescent quantitative PCR (Polymerase Chain Reaction) kit

Abstract

The utility model discloses a one-step fluorescent quantitative PCR (Polymerase Chain Reaction) kit which comprises a kit body and a kit cover, the kit body and the kit cover are mutually clamped, fixing parts for fixing reagent bottles are arranged in the kit body and the kit cover, buffer parts for buffering the reagent bottles when the reagent bottles are vibrated are arranged on the fixing parts, and the buffer parts are arranged on the kit cover. The fixing parts are arranged in the kit cover and the kit body, the buffering parts are arranged in the fixing parts, reagent bottles can be fixed and buffered from the two ends, the buffering effect is improved, and the kit cover and the kit body are connected in a clamped mode so that sealing and taking can be convenient.

Description

Technical Field

[0001] The utility model relates to a one-step fluorescent quantitative PCR kit and belongs to the field of PCR kits. Background Art

[0002] In order to better preserve PCR reagents during transportation and avoid damage to reagent packaging bottles caused by impact, which may lead to contamination of reagents, a Chinese patent document with announcement number CN209481639U discloses a one-step fluorescent quantitative PCR kit, which fixes the reagent bottle by a reagent bottle buffer bottom plate, a sleeve and an intermediate support plate, but does not limit and fix the upper end of the reagent bottle. When vibrated, the reagent bottle will still move up and down, and the buffering effect is not good. In addition, the packaging box body and the box body sealing cover are connected by hot melting, which is very cumbersome when sealing and taking. In order to solve the above problems, the utility model provides a one-step fluorescent quantitative PCR kit. Utility Model Content

[0003] In view of the problems existing in the above-mentioned prior art, the utility model provides a one-step fluorescent quantitative PCR kit, wherein fixing parts are arranged inside the box cover and the box body, and a buffer part is arranged inside the fixing part, so that the reagent bottle can be fixed and buffered from both ends, thereby improving the buffering effect, and the box cover and the box body are snap-connected in a form of convenient sealing and access.

[0004] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a one-step fluorescent quantitative PCR kit, comprising a box body and a box cover, wherein the box body and the box cover are engaged with each other, and when the box cover and the box body are engaged with each other, the opening of the box body can be sealed, and the inside of the box body and the box cover are both equipped with fixing parts for fixing reagent bottles, and the reagent bottles can be fixed from the upper and lower ends of the reagent bottles, and a buffer part for buffering the reagent bottles when the reagent bottles are vibrated is installed on the fixing part, and the buffer part can buffer the upper and lower ends of the reagent bottles in the axial direction of the reagent bottles.

[0005] Preferably, a limiting portion is fixedly connected to the interior of the box body, and the limiting portion is located above the fixed portion inside the box body, and the limiting portion can limit the middle part of the reagent bottle.

[0006] Preferably, the fixing portion comprises a fixing block, and a plurality of insertion holes are provided on the fixing block, and the size of the insertion holes is adapted to the size of the reagent bottle.

[0007] Preferably, the limiting portion comprises a fixing plate, a plurality of through holes are formed on the fixing plate, the fixing plate is fixedly connected to the interior of the box body, and the size of the through holes is adapted to the size of the reagent bottle.

[0008] Preferably, the buffer portion includes an elastic member and a buffer plate, the buffer plate and the reagent bottle are fitted together, the buffer plate is slidably connected to the inside of the socket, and the buffer plate is connected to the inner wall of the socket through the elastic member, the elastic member has an elastic force to press the buffer plate to the outside of the corresponding socket, and under the elastic force of the elastic member, the buffer plate will fit tightly with the corresponding reagent bottle.

[0009] Preferably, the elastic member is a spring, and the elastic member may also be a spring sheet or a rubber block.

[0010] Preferably, buffer washers are fixedly connected to the inner side walls of the insertion hole and the through hole, and the buffer washers can also buffer the reagent bottle in the radial direction of the reagent bottle when it is vibrated.

[0011] Preferably, an insertion frame is fixedly connected to the opening of the box body, and a space adapted to the insertion frame is reserved between the inner wall of the box cover and the corresponding fixing portion. The provision of the insertion frame not only enables the fixing portion on the box cover to be plugged into the reagent bottle when the staff installs the box cover.

[0012] Preferably, a slot is provided on the side of the box body, and a plug plate adapted to the slot is fixedly connected to the box cover, the plug plate can produce elastic deformation, a card strip is fixedly connected to the slot wall of the slot, and a bayonet adapted to the card strip is provided on the plug plate, and the bayonet and the card strip are in an interference fit relationship, and an empty slot is provided on the outer side of the plug plate, and the setting of the empty slot makes it convenient for the staff to pry open the plug plate when removing the box cover.

[0013] Compared with the prior art, the beneficial effects of the utility model are as follows:

[0014] 1. The inside of the box body and the box cover are both provided with a fixing part, and a buffer part is provided inside the fixing part, which can fix and buffer the reagent bottle in the axial direction, thereby improving the buffering effect;

[0015] 2. Buffer washers are fixed on the inner walls of the jack and the through hole, which can fix and buffer the reagent in the radial direction;

[0016] 3. The setting of the plug plate and the slot can make the box cover and the box body stably connected together, and the box cover can be easily disassembled and assembled, so that it is convenient for the staff to take the reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of the utility model.

[0018] Figure 2 It is an exploded view of the box cover and the box body of the utility model.

[0019] Figure 3It is a cross-sectional view of the utility model.

[0020] Figure 4 For this utility model Figure 2 Exploded diagram.

[0021] In the figure: 1, box body, 2, box cover, 3, fixing part, 301, fixing block, 302, plug hole, 4, buffer part, 401, elastic member, 402, buffer plate, 5, limiting part, 501, fixing plate, 502, through hole, 6, buffer gasket, 7, plug-in frame, 8, slot, 9, plug-in board, 10, bayonet, 11, card strip, 12, empty slot. DETAILED DESCRIPTION

[0022] The present invention is described below with specific embodiments, but is not intended to be a limitation of the present invention.

[0023] Embodiment 1

[0024] In this embodiment, a one-step fluorescent quantitative PCR kit is provided, such as Figure 1 , Figure 3 and Figure 4 As shown, it includes a box body 1 and a box cover 2, and the box body 1 and the box cover 2 are clamped together. When the box cover 2 and the box body 1 are clamped together, the opening of the box body 1 can be sealed, so that the interior of the box body 1 is in a sealed state, and the reagent bottles stored in the box body 1 are protected. The interiors of the box body 1 and the box cover 2 are both equipped with fixing parts 3 for fixing the reagent bottles, and the reagent bottles can be fixed from the upper and lower ends of the reagent bottles to better maintain a stable state inside the box body 1. The fixing part 3 is equipped with a buffer part 4 for buffering the reagent bottle when the reagent bottle is vibrated. The buffer part 4 can buffer the upper and lower ends of the reagent bottle in the axial direction of the reagent bottle, thereby ensuring that the reagent bottle will not be damaged when vibration occurs.

[0025] The fixing part 3 includes a fixing block 301 , which is provided with a plurality of sockets 302 . The fixing block 301 is fixedly connected to the box body 1 or the box cover 2 . The size of the sockets 302 matches the size of the reagent bottle, and the end of the reagent bottle can be inserted into the sockets 302 .

[0026] The buffer portion 4 includes an elastic member 401 and a buffer plate 402. The buffer plate 402 fits closely with the reagent bottle. The buffer plate 402 is slidably connected to the inside of the socket 302, and the buffer plate 402 is connected to the inner wall of the socket 302 through the elastic member 401. The elastic member 401 has an elastic force that presses the buffer plate 402 against the outside of the corresponding socket 302. Under the elastic force of the elastic member 401, the buffer plate 402 will fit closely with the corresponding reagent bottle. When vibrated, the elastic members 401 at both ends of the reagent bottle will produce corresponding elastic deformation, thereby providing a buffer for the reagent bottle.

[0027] The elastic member 401 is a spring, and the elastic member 401 can also be a spring sheet or a rubber block.

[0028] like Figure 2 As shown, an insertion frame 7 is fixedly connected to the opening of the box body 1, and a space adapted to the insertion frame 7 is reserved between the inner wall of the box cover 2 and the corresponding fixing portion 3. The provision of the insertion frame 7 not only enables the staff to make the fixing portion 3 on the box cover 2 plug into the reagent bottle when installing the box cover 2, but also improves the sealing between the box cover 2 and the box body 1.

[0029] A slot 8 is provided on the side of the box body 1, and a plug plate 9 matched with the slot 8 is fixedly connected to the box cover 2, and the plug plate 9 can produce elastic deformation. A card strip 11 is fixedly connected to the groove wall of the slot 8, and a bayonet 10 matched with the card strip 11 is provided on the plug plate 9. The bayonet 10 and the card strip 11 are in an interference fit relationship. When the box cover 2 is installed on the box body 1, the plug plate 9 will be inserted into the inside of the slot 8. At this time, the card strip 11 will be engaged with the bayonet 10, so that the box cover 2 and the box body 1 are engaged with each other. An empty groove 12 is provided on the outer side of the plug plate 9. The setting of the empty groove 12 can make it convenient for the staff to pry open the plug plate 9 when removing the box cover 2, so that the bayonet 10 and the card strip 11 are disengaged, and then the box cover 2 can be removed.

[0030] Embodiment 2

[0031] In order to further improve the buffering effect on the reagent bottle, a limiting part 5 is provided, specifically, as follows:

[0032] The box body 1 is internally fixedly connected with a limiting portion 5, and the limiting portion 5 is located above the fixing portion 3 inside the box body 1. The limiting portion 5 can limit the middle of the reagent bottle, so that the reagent bottle can be further stabilized inside the box body 1.

[0033] The limiting portion 5 includes a fixing plate 501, which is provided with a plurality of through holes 502. The fixing plate 501 is fixedly connected to the interior of the box body 1. The size of the through holes 502 is adapted to the size of the reagent bottle, and the reagent bottle can pass through the through holes 502. The number and position of the through holes 502 correspond one-to-one to the number and position of the jacks 302.

[0034] The inner side walls of the insertion hole 302 and the through hole 502 are fixedly connected with a buffer gasket 6. When the reagent bottle is subjected to vibration, the buffer gasket 6 can also buffer the reagent bottle in the radial direction, which can further improve the buffering effect.

[0035] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A one-step fluorescent quantitative PCR kit, characterized in that: The invention comprises a box body (1) and a box cover (2), wherein the box body (1) and the box cover (2) are mutually clamped, and a fixing part (3) for fixing a reagent bottle is installed inside the box body (1) and the box cover (2), and a buffer part (4) for buffering the reagent bottle when the reagent bottle is vibrated is installed on the fixing part (3).

2. A one-step fluorescent quantitative PCR kit according to claim 1, characterized in that: The interior of the box body (1) is fixedly connected to a limiting portion (5), and the limiting portion (5) is located above the fixing portion (3) inside the box body (1).

3. A one-step fluorescent quantitative PCR kit according to claim 2, characterized in that: The fixing portion (3) comprises a fixing block (301), and a plurality of insertion holes (302) are provided on the fixing block (301).

4. A one-step fluorescent quantitative PCR kit according to claim 3, characterized in that: The limiting portion (5) comprises a fixing plate (501), and a plurality of through holes (502) are formed on the fixing plate (501).

5. A one-step fluorescent quantitative PCR kit according to claim 3, characterized in that: The buffer portion (4) comprises an elastic member (401) and a buffer plate (402); the buffer plate (402) is slidably connected to the inside of the plug hole (302); the buffer plate (402) is connected to the inner side wall of the plug hole (302) via the elastic member (401); and the elastic member (401) has an elastic force for pressing the buffer plate (402) against the outside of the corresponding plug hole (302).

6. A one-step fluorescent quantitative PCR kit according to claim 5, characterized in that: The elastic member (401) is a spring.

7. A one-step fluorescent quantitative PCR kit according to claim 4, characterized in that: Buffer washers (6) are fixedly connected to the inner side walls of the insertion hole (302) and the through hole (502).

8. The one-step fluorescent quantitative PCR kit according to claim 1, characterized in that: An insertion frame (7) is fixedly connected to the opening of the box body (1), and a space adapted to the insertion frame (7) is reserved between the inner side wall of the box cover (2) and the corresponding fixing portion (3).

9. The one-step fluorescent quantitative PCR kit according to claim 8, characterized in that: A slot (8) is provided on the side of the box body (1); a plug plate (9) adapted to the slot (8) is fixedly connected to the box cover (2); a clamping strip (11) is fixedly connected to the slot wall of the slot (8); a clamping opening (10) adapted to the clamping strip (11) is provided on the plug plate (9); and an empty slot (12) is provided on the outer side of the plug plate (9).

Images