PCR (Polymerase Chain Reaction) microfluidic consumable
By designing PCR microfluidic consumables with storage cavity, runner, injection hole and microreaction chamber, the limitations of existing PCR consumables in multiple target detection operations are solved, and efficient multiple detection operations are achieved, which significantly improves the practicality of the consumables.
Patent Information
- Application Number
- CN202421079021.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-17
- Publication Date
- 2025-02-28
- Estimated Expiration
- 2034-05-17
Smart Images
Figure 00000000_0000_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of pathogen, gene mutation and gene polymorphism detection consumables, in particular to a PCR microfluidic consumable. Background Art
[0002] In the contemporary medical experimental testing process, a large number of medical instruments and medical equipment are needed, as well as a large number of disposable consumables. In the process of target detection of pathogens, PCR consumables are needed.
[0003] Existing PCR consumables have limitations in the detection of multiple targets in the same batch, and their overall practicality needs to be improved. Utility Model Content
[0004] The utility model aims to provide a PCR microfluidic consumable material which can perform multiple target detection operations in the same batch and has high practicality.
[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0006] A PCR microfluidic consumable comprises a PCR body, wherein two groups of microfluidic channels are arranged on the outer surface of the PCR body, each group of the microfluidic channels comprises a storage cavity, a flow channel is arranged at one end of the storage cavity, a plurality of injection holes are arranged on symmetrical sides of the flow channel, a micro reaction chamber is arranged at one end of the injection hole, one end of the flow channel is communicated with the interior of the storage cavity, one end of the injection hole is communicated with the interior of the flow channel, and the other end of the injection hole is communicated with the interior of the micro reaction chamber.
[0007] By adopting the above technical solution, multiple target detection operations can be performed in the same batch.
[0008] Furthermore, the cross section of the flow channel is trapezoidal.
[0009] By adopting the above technical solution, it is ensured that the sample liquid can flow in the flow channel.
[0010] Furthermore, the cross section of the injection hole is trapezoidal.
[0011] By adopting the above technical solution, it is ensured that the sample liquid can flow in the injection hole.
[0012] Furthermore, a snap groove is provided on the side surface of the PCR body.
[0013] By adopting the above technical solution, it is convenient to fix the edge of the PET film.
[0014] Furthermore, a binding elastic cord is sleeved inside the buckle slot, and two flexible traction belts are sleeved and fixed outside the binding elastic cord.
[0015] By adopting the above technical solution, the flexible traction belt can be used to pull the binding elastic rope, and then the binding elastic rope can be used to bind the PET film.
[0016] Furthermore, arc-shaped chamfer structures are arranged at the edges of four sides of the upper surface of the PCR body.
[0017] By adopting the above technical solution, the tightness of the PET film attached to the upper surface of the PCR body can be effectively improved.
[0018] In summary, the beneficial technical effects of the utility model are:
[0019] 1. The utility model can use the storage cavity to collect the sample liquid, and then make the sample liquid flow into the injection hole along the flow channel, and then flow into the micro-reaction chamber along the injection hole. Since multiple micro-reaction chambers are provided, three to five pathogenic microorganisms can be stored inside each micro-reaction chamber, so that multiple target detection operations can be performed on the same consumable, and the practicality is effectively enhanced;
[0020] 2. When in use, the utility model can cover the PET film on the upper surface of the PCR body, and then place the edge of the film on the outside of the PCR body. Then, the elastic rope is sleeved on the outside of the PET film and buckled into the inside of the buckle groove, so that the PET film can be tightly attached to the upper surface of the PCR body, and the overall stability is effectively improved. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 It is a three-dimensional structural schematic diagram of the utility model.
[0022] In the figure: 1. PCR body; 2. Storage cavity; 3. Flow channel; 4. Injection hole; 5. Micro reaction chamber; 6. Buckle groove; 7. Binding elastic rope; 8. Flexible traction belt; 9. Arc chamfer structure; 10. Microfluidic channel. DETAILED DESCRIPTION
[0023] The method of the utility model is further described in detail below in conjunction with the accompanying drawings.
[0024] See attached Figure 1A PCR microfluidic consumable comprises a PCR body 1, two groups of microfluidic channels 10 are arranged on the outer surface of the PCR body 1, each group of microfluidic channels 10 comprises a storage cavity 2, a flow channel 3 is arranged at one end of the storage cavity 2, a plurality of injection holes 4 are arranged on both symmetrical sides of the flow channel 3, a micro reaction chamber 5 is arranged at one end of the injection hole 4, one end of the flow channel 3 is communicated with the interior of the storage cavity 2, one end of the injection hole 4 is communicated with the interior of the flow channel 3, and the other end of the injection hole 4 is communicated with the interior of the micro reaction chamber 5, the cross section of the flow channel 3 is trapezoidal, and the cross section of the injection hole 4 is trapezoidal, wherein the storage cavity 2 can be used to collect sample liquid, and then the sample liquid flows into the injection hole 4 along the flow channel 3, and then flows into the micro reaction chamber 5 along the injection hole 4, since a plurality of micro reaction chambers 5 are arranged, three to five pathogenic microorganisms can be stored in each micro reaction chamber 5, so that multiple target detection operations can be performed on the same consumable, and the practicality is effectively enhanced.
[0025] Reference Figure 1 A buckle groove 6 is provided on the side surface of the PCR body 1, and a binding elastic rope 7 is sleeved inside the buckle groove 6, and two flexible traction belts 8 are sleeved and fixed outside the binding elastic rope 7. Arc chamfer structures 9 are provided at the four side edges of the upper surface of the PCR body 1. When in use, the PET film can be covered on the upper surface of the PCR body 1, and then the edge position of the film is placed outside the surrounding of the PCR body 1, and then the binding elastic rope 6 is sleeved on the outside of the PET film and buckled into the inside of the buckle groove 6, so that the PET film can be tightly attached to the upper surface of the PCR body 1, and the overall stability is effectively improved.
[0026] Working principle: When in use, the sample liquid to be processed is placed in the storage chamber 2, and then the centrifugal force is used to make the sample liquid in the storage chamber 2 flow into the interior of the flow channel 3, and then flow into the interior of the injection hole 4, and finally flow into the micro-reaction chamber 5, and then the PET film is covered on the upper surface of the PCR body 1, and then the edge position of the film is disposed on the outside of the PCR body 1, and then the binding elastic rope 6 is sleeved on the outside of the PET film and buckled into the inside of the buckle groove 6, so that the PET film can be tightly attached to the upper surface of the PCR body 1. Since a plurality of micro-reaction chambers 5 are provided, three to five pathogenic microorganisms can be stored in each micro-reaction chamber 5, so that multiple target detection operations can be performed on the same consumables, and the practicality is effectively enhanced.
[0027] The embodiments of this specific implementation method are all preferred embodiments of the utility model, and are not intended to limit the protection scope of the utility model. Therefore, all equivalent changes made based on the structure, shape, and principle of the utility model should be included in the protection scope of the utility model.
Claims
1. A PCR microfluidic consumable, comprising a PCR body (1), characterized in that: Two groups of microfluidic channels (10) are arranged on the outer surface of the PCR body (1), each group of the microfluidic channels (10) includes a storage cavity (2), one end of the storage cavity (2) is provided with a flow channel (3), and symmetrically two sides of the flow channel (3) are provided with a plurality of injection holes (4), and one end of the injection hole (4) is provided with a micro reaction chamber (5), one end of the flow channel (3) is communicated with the interior of the storage cavity (2), one end of the injection hole (4) is communicated with the interior of the flow channel (3), and the other end of the injection hole (4) is communicated with the interior of the micro reaction chamber (5).
2. A PCR microfluidic consumable according to claim 1, characterized in that: The cross section of the flow channel (3) is trapezoidal.
3. A PCR microfluidic consumable according to claim 1, characterized in that: The cross section of the injection hole (4) is trapezoidal.
4. A PCR microfluidic consumable according to claim 1, characterized in that: A snap groove (6) is provided on the side surface of the PCR body (1).
5. A PCR microfluidic consumable according to claim 4, characterized in that: The interior of the buckle slot (6) is sleeved with a binding elastic cord (7), and the exterior of the binding elastic cord (7) is sleeved and fixed with two flexible traction belts (8).
6. A PCR microfluidic consumable according to claim 1, characterized in that: Arc-shaped chamfer structures (9) are provided at the four side edges of the upper surface of the PCR body (1).