Liquid-based cell slide preparation liquid path

Abstract

The utility model discloses a liquid-based cell preparation liquid path which comprises at least one reagent needle group which is respectively connected with a staining reagent A liquid container, a staining reagent B liquid container, a cleaning liquid container, a transparent liquid container and a buffer liquid container through an electromagnetic valve I, an electromagnetic valve II, an electromagnetic valve III, an electromagnetic valve IV, an electromagnetic valve V and a pipeline, the dyeing reagent A liquid container, the dyeing reagent B liquid container, the cleaning liquid container, the transparent liquid container and the buffer liquid container are connected with an air compression device through pipelines and electromagnetic reversing valves; the first electromagnetic valve, the second electromagnetic valve, the third electromagnetic valve, the fourth electromagnetic valve, the fifth electromagnetic valve and the electromagnetic reversing valve are electrically connected with a control device. According to the utility model, quantitative repeated liquid adding at each reagent needle position is realized, the use quantity of liquid path pumps and control electromagnetic valves is reduced, and the liquid path is simplified. Faults of a liquid path system are reduced, and the using performance is more stable.

Description

Technical Field

[0001] The utility model relates to the technical field of cell detection, in particular to a liquid-based cell preparation liquid circuit. Background Art

[0002] In clinical diagnosis, liquid-based cell samples such as urine, pleural effusion, and cervical cell preservation fluid are often purified and enriched in test tubes through density gradient centrifugation, and then transferred to glass slides through the principle of charge attraction, and stained, immunologically, genetically, and other markers are performed for readers to perform morphological identification under a microscope and make clinical diagnoses. The process of transferring cells from liquid samples to glass slides and labeling them is called liquid-based cell preparation. One of the important links is the preparation of liquid-based thin-layer cells, which is to transfer useful cells to the preparation body to make a cell smear with a clear background, neat and uniform cells, and a single layer of flattened cells for inspection and observation by researchers.

[0003] In order to obtain a cell smear with a clear background, neat and uniform cells, and a single layer of flat cells, it is necessary to add various reagents such as staining liquid, separation liquid, transparent liquid, buffer solution, etc. to separate the liquid-based cells. As a result, a large number of various hydraulic pumps and solenoid valves will be used. This will cause the liquid circuit design to be too complicated, the stability of use is not easy to ensure, and the liquid circuit is prone to blockage and cannot be used normally. The large-scale use of various hydraulic pumps and solenoid valves will make the control system too complicated and it will be difficult to troubleshoot faults. Utility Model Content

[0004] In order to solve the above problems, the utility model provides a liquid-based cell preparation liquid circuit, which reduces the number of liquid circuit pumps and control solenoid valves used and simplifies the liquid circuit.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: a liquid-based cell preparation liquid circuit, including at least one group of reagent needle groups, and the reagent needle groups are respectively connected to the staining reagent A liquid container, the staining reagent B liquid container, the cleaning liquid container, the transparent liquid container, and the buffer solution container through solenoid valve one, solenoid valve two, solenoid valve three, solenoid valve four, solenoid valve five and pipelines; the staining reagent A liquid container, the staining reagent B liquid container, the cleaning liquid container, the transparent liquid container, and the buffer solution container are connected to a compressed air device through pipelines and solenoid reversing valves; the solenoid valve one, the solenoid valve two, the solenoid valve three, the solenoid valve four, the solenoid valve five, and the solenoid reversing valve are respectively electrically connected to a control device.

[0006] As a preferred technical solution of the utility model: the reagent needle group is connected to the sedimentation bin through a filtering device and a pipeline.

[0007] As a preferred technical solution of the utility model: the filtering device is a diaphragm pump.

[0008] As a preferred technical solution of the utility model: the sedimentation bin is connected to the separation liquid container through an electromagnetic valve eleven, and the electromagnetic valve eleven is electrically connected to the control device; the separation liquid container is connected to the compressed air device through a pipeline and an electromagnetic reversing valve.

[0009] As a preferred technical solution of the utility model: there are four reagent needle groups, namely reagent needle group one, reagent needle group two, reagent needle group three and reagent needle group four.

[0010] As an optimal technical solution of the utility model: the reagent needle group one and the reagent needle group two are respectively connected to the staining reagent A liquid container, the staining reagent B liquid container, the cleaning liquid container, the transparent liquid container, and the buffer solution container through the solenoid valve one, the solenoid valve two, the solenoid valve three, the solenoid valve four, the solenoid valve five and the pipeline; the reagent needle group three and the reagent needle group four are respectively connected to the staining reagent A liquid container, the staining reagent B liquid container, the cleaning liquid container, the transparent liquid container, and the buffer solution container through the solenoid valve six, the solenoid valve seven, the solenoid valve eight, the solenoid valve nine, the solenoid valve ten and the pipeline; the solenoid valve six, the solenoid valve seven, the solenoid valve eight, the solenoid valve nine, and the solenoid valve ten are respectively electrically connected to the control device.

[0011] As a preferred technical solution of the utility model: the solenoid valve one, solenoid valve two, solenoid valve three, solenoid valve four, solenoid valve five, solenoid valve six, solenoid valve seven, solenoid valve eight, solenoid valve nine, and solenoid valve ten are two-way valves.

[0012] As a preferred technical solution of the utility model: the reagent needle group 1 and the reagent needle group 2 are connected to the sedimentation bin through the filter device 1 and the pipeline; the reagent needle group 3 and the reagent needle group 4 are connected to the sedimentation bin through the filter device 2 and the pipeline.

[0013] The utility model has the following beneficial effects:

[0014] 1. Realize quantitative multiple liquid addition at each reagent needle position, while reducing the number of liquid circuit pumps and control solenoid valves used and simplifying the liquid circuit.

[0015] 2. Air pressure provides driving force, which can reduce the failure of the hydraulic system and make the performance more stable.

[0016] 3. The reagent needle group can centrally arrange various reagent needles to reduce the physical size, save space, optimize the structural design, and make it easier to miniaturize and lightweight the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is the overall flow chart of the utility model;

[0018] Figure 2 is a schematic diagram of the structure of the reagent needle group;

[0019] Figure 1-Figure 2 Among them, 1. Staining reagent A liquid container; 2. Staining reagent B liquid container; 3. Cleaning liquid container; 4. Transparent liquid container; 5. Buffer solution container; 6. Separation liquid container; 7. Sedimentation chamber; 8. Tee; 9. A liquid reagent needle; 10. B liquid reagent needle; 11. Cleaning liquid reagent needle; 12. Transparent liquid reagent needle; 13. Buffer solution reagent needle; 14. Waste liquid needle. DETAILED DESCRIPTION

[0020] In order to make the purpose and advantages of the utility model more clear, the utility model is further described in detail in conjunction with the embodiments below. It should be understood that the specific embodiments described here are only used to explain the utility model, and are not used to limit the utility model.

[0021] like Figure 1-Figure 2 As shown, an embodiment of the utility model provides a liquid-based cell preparation liquid circuit, including at least one group of reagent needle groups, the reagent needle group includes a liquid A reagent needle 9, a liquid B reagent needle 10, a cleaning liquid reagent needle 11, a transparent liquid reagent needle 12, a buffer solution reagent needle 13, and a waste liquid needle 14. The reagent needle group centrally arranges various reagent needles to reduce the physical size and save space. The structural design will be more optimized, making it easier to achieve miniaturization and lightweight of the equipment. A liquid reagent needle 9, B liquid reagent needle 10, cleaning liquid reagent needle 11, transparent liquid reagent needle 12, buffer reagent needle 13 are connected to dyeing reagent A liquid container 1, dyeing reagent B liquid container 2, cleaning liquid container 3, transparent liquid container 4, buffer container 5 through electromagnetic valve 1, electromagnetic valve 2, electromagnetic valve 3, electromagnetic valve 4, electromagnetic valve 5 and pipelines respectively; dyeing reagent A liquid container 1, dyeing reagent B liquid container 2, cleaning liquid container 3, transparent liquid container 4, buffer container 5 are connected to the compressed air device through pipelines and electromagnetic reversing valves; electromagnetic valve 1, electromagnetic valve 2, electromagnetic valve 3, electromagnetic valve 4, electromagnetic valve 5, and electromagnetic reversing valve are electrically connected to the control device respectively. The control device is PIC. The compressed air device provides positive pressure to dyeing reagent A liquid container 1, dyeing reagent B liquid container 2, cleaning liquid container 3, transparent liquid container 4, and buffer container 5, so that the liquids therein are added to the corresponding reagent needles in the reagent needle group respectively. The driving force provided by air pressure can reduce the failure of the liquid circuit system and make the use performance more stable. The control system realizes quantitative multiple liquid addition at each reagent needle position by controlling solenoid valve one, solenoid valve two, solenoid valve three, solenoid valve four, solenoid valve five and solenoid reversing valve, while also reducing the number of liquid circuit pumps and control solenoid valves used and simplifying the liquid circuit.

[0022] The waste liquid needle 14 of the reagent needle group is connected to the sedimentation bin 7 through a filter device and a pipeline. The filter device is preferably a diaphragm pump, which discharges the waste liquid of the reagent needle group into the sedimentation bin 7. The sedimentation bin 7 is connected to the separation liquid container 6 through an electromagnetic valve 11, and the separation liquid is placed in the separation liquid container 6. The electromagnetic valve 11 is electrically connected to the control device, and the separation liquid container 6 is connected to the compressed air device, and the separation liquid is added to the sedimentation bin 7 by controlling the electromagnetic valve 11.

[0023] In this embodiment, it is preferred that there are four reagent needle groups; they are reagent needle group 1, reagent needle group 2, reagent needle group 3 and reagent needle group 4. Reagent needle group 1 and reagent needle group 2 are connected to the dyeing reagent A liquid container 1, dyeing reagent B liquid container 2, cleaning liquid container 3, transparent liquid container 4 and buffer solution container 5 through electromagnetic valve 1, electromagnetic valve 2, electromagnetic valve 3, electromagnetic valve 4, electromagnetic valve 5 and pipelines, tees 8 and other equipment; reagent needle group 3 and reagent needle group 4 are connected to the dyeing reagent A liquid container 1, dyeing reagent B liquid container 2, cleaning liquid container 3, transparent liquid container 4 and buffer solution container 5 through electromagnetic valve 6, electromagnetic valve 7, electromagnetic valve 8, electromagnetic valve 9, electromagnetic valve 10 and pipelines, tees 8 and other equipment; electromagnetic valve 6, electromagnetic valve 7, electromagnetic valve 8, electromagnetic valve 9 and electromagnetic valve 10 are electrically connected to the control device. Electromagnetic valve 1, electromagnetic valve 2, electromagnetic valve 3, electromagnetic valve 4, electromagnetic valve 5, electromagnetic valve 6, electromagnetic valve 7, electromagnetic valve 8, electromagnetic valve 9 and electromagnetic valve 10 are two-way valves. The two groups of reagent needle groups share a solenoid valve control, which further reduces the number of liquid circuit pumps and control solenoid valves used and simplifies the liquid circuit. Of course, it is understandable that in other embodiments, after being equipped with corresponding solenoid valves, pipelines, tees 8 and other equipment, the number of reagent needle groups can be expanded to six groups, eight groups, etc. In order to simplify the pipeline for waste liquid discharge, reagent needle group 1 and reagent needle group 2 are connected to sedimentation bin 7 through filter device 1 and pipelines; reagent needle group 3 and reagent needle group 4 are connected to sedimentation bin 7 through filter device 2 and pipelines.

[0024] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", and "third" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0025] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A liquid-based cell preparation liquid circuit, characterized in that: It comprises at least one reagent needle group, which is respectively connected to a dyeing reagent A liquid container, a dyeing reagent B liquid container, a cleaning liquid container, a transparent liquid container and a buffer solution container through solenoid valve 1, solenoid valve 2, solenoid valve 3, solenoid valve 4, solenoid valve 5 and pipelines; the dyeing reagent A liquid container, the dyeing reagent B liquid container, the cleaning liquid container, the transparent liquid container and the buffer solution container are connected to a compressed air device through pipelines and solenoid reversing valves; the solenoid valve 1, the solenoid valve 2, the solenoid valve 3, the solenoid valve 4, the solenoid valve 5 and the solenoid reversing valve are respectively electrically connected to a control device.

2. The liquid-based cell preparation liquid circuit according to claim 1, characterized in that: The reagent needle group is connected to the sedimentation bin through a filtering device and a pipeline.

3. The liquid-based cell preparation liquid circuit according to claim 2, characterized in that: The filtering device is a diaphragm pump.

4. The liquid-based cell preparation liquid circuit according to claim 2, characterized in that: The sedimentation bin is connected to the separation liquid container through an electromagnetic valve eleven, and the electromagnetic valve eleven is electrically connected to the control device; the separation liquid container is connected to the compressed air device through a pipeline and an electromagnetic reversing valve.

5. The liquid-based cell preparation liquid circuit according to claim 1, characterized in that: There are four groups of reagent needle groups, namely reagent needle group one, reagent needle group two, reagent needle group three and reagent needle group four.

6. The liquid-based cell preparation liquid circuit according to claim 5, characterized in that: The reagent needle group one and the reagent needle group two are connected to the dyeing reagent A liquid container, the dyeing reagent B liquid container, the cleaning liquid container, the transparent liquid container, and the buffer solution container respectively through the solenoid valve one, the solenoid valve two, the solenoid valve three, the solenoid valve four, the solenoid valve five and the pipeline; the reagent needle group three and the reagent needle group four are connected to the dyeing reagent A liquid container, the dyeing reagent B liquid container, the cleaning liquid container, the transparent liquid container, and the buffer solution container respectively through the solenoid valve six, the solenoid valve seven, the solenoid valve eight, the solenoid valve nine, the solenoid valve ten and the pipeline; the solenoid valve six, the solenoid valve seven, the solenoid valve eight, the solenoid valve nine, and the solenoid valve ten are electrically connected to the control device respectively.

7. The liquid-based cell preparation liquid circuit according to claim 6, characterized in that: The solenoid valve 1, the solenoid valve 2, the solenoid valve 3, the solenoid valve 4, the solenoid valve 5, the solenoid valve 6, the solenoid valve 7, the solenoid valve 8, the solenoid valve 9 and the solenoid valve 10 are two-way valves.

8. The liquid-based cell preparation liquid circuit according to claim 5, characterized in that: The reagent needle group 1 and the reagent needle group 2 are connected to the sedimentation bin through the filter device 1 and the pipeline; the reagent needle group 3 and the reagent needle group 4 are connected to the sedimentation bin through the filter device 2 and the pipeline.

Images