Full-automatic cell harvesting instrument

Abstract

The utility model discloses a full-automatic cell harvesting instrument which comprises a frame, a liquid injection mechanism, a liquid suction mechanism, a centrifugal mechanism and an oscillating mechanism, the liquid injection mechanism, the liquid suction mechanism, the centrifugal mechanism and the oscillating mechanism are located in the frame, the frame comprises a cross beam and a workbench, the cross beam is located above the workbench, and the liquid injection mechanism and the liquid suction mechanism are fixed on the cross beam. The centrifugal mechanism is fixed to the workbench and located below the workbench, the liquid injection needle translation assembly and the liquid injection needle lifting assembly horizontally move on the cross beam, the liquid injection needle lifting assembly vertically moves relative to the liquid injection needle translation assembly, the oscillating mechanism is located below the workbench, and the centrifugal mechanism comprises a plurality of centrifugal test tube racks. The vibration rod drives the centrifugal test tube rack to vibrate through the rotation of the eccentric ball seat. The liquid injection mechanism and the liquid suction mechanism are fixed to the cross beam, the centrifugal mechanism, the oscillation mechanism and the supporting mechanism are fixed to the lower portion of the workbench, cooperative work of multiple mechanisms is achieved, an integrated working platform is achieved, and cell chromosome collection work is completed.

Description

Technical Field

[0001] The utility model relates to the technical field of bioengineering equipment, in particular to a full-automatic cell harvester. Background Art

[0002] The fully automatic cell harvester is mainly used for cytogenetics chromosome karyotype analysis. It can automatically complete the harvesting of metaphase chromosomes of peripheral blood, bone marrow, amniotic fluid and other samples through the cooperation of multiple mechanisms such as injection mechanism, liquid aspiration mechanism, centrifugation mechanism and oscillation mechanism, reduce the error caused by manual operation, and improve the harvesting efficiency and accuracy. It usually includes injection mechanism, liquid transfer mechanism, centrifugation mechanism and oscillation mechanism, involving the cooperation of multiple mechanisms.

[0003] In the existing technology, the various mechanisms in the laboratory are often placed on the same working plane, and the experimenters manually operate them and move back and forth between the mechanisms to complete the cell chromosome collection work. Therefore, in order to promote better collaboration among the mechanisms, it is particularly important to build an integrated and miniaturized work platform. Utility Model Content

[0004] In order to overcome the deficiencies of the prior art, the purpose of the utility model is to provide an integrated fully automatic cell harvester that can facilitate the process and improve work efficiency.

[0005] The purpose of the utility model is achieved by the following technical solutions:

[0006] A fully automatic cell harvester comprises a frame, and also comprises an injection mechanism, a liquid suction mechanism, a centrifugal mechanism and an oscillating mechanism, wherein the injection mechanism, the liquid suction mechanism, the centrifugal mechanism and the oscillating mechanism are located in the frame, the frame comprises a crossbeam and a workbench, the crossbeam is located above the workbench, the injection mechanism and the liquid suction mechanism are fixed on the crossbeam, the centrifugal mechanism is fixed on the workbench and located below the workbench, the injection mechanism comprises an injection needle translation assembly, an injection needle lifting assembly and an injection needle group, the injection needle translation assembly and the injection needle lifting assembly move horizontally on the crossbeam, the injection needle lifting assembly moves vertically relative to the injection needle translation assembly, the oscillating mechanism is located below the workbench, the centrifugal mechanism comprises a plurality of centrifugal test tube racks, the centrifugal test tube racks comprise a vibration rod and a ball shaft, the ball shaft is located below the vibration rod, the oscillating mechanism comprises an eccentric ball seat, the vibration rod is plugged with the eccentric ball seat through the ball shaft, and the vibration rod drives the centrifugal test tube rack to oscillate through the rotation of the eccentric ball seat.

[0007] Furthermore, the injection needle translation assembly includes a guide rail fixing plate, a first vertical plate, a first slide rail and a first slider, the guide rail fixing plate and the first vertical plate are respectively fixed on the cross beam, the guide rail fixing plate and the first vertical plate are respectively fixed with the first slide rail, the injection needle lifting assembly includes a first slider adapter plate, the first sliders are respectively fixed at both ends of the first slider adapter plate, the first slide rail is slidably connected to the first slider, the injection needle lifting assembly also includes an injection electric cylinder and an injection linear module, the injection linear module is slidably connected to the injection electric cylinder.

[0008] Furthermore, the centrifugal mechanism includes a centrifugal chamber body, a centrifugal chamber cover, a centrifugal turntable and a centrifugal motor, the centrifugal test tube rack and the centrifugal turntable are located in the centrifugal chamber body, the centrifugal test tube rack is distributed along the circumference of the centrifugal turntable, the centrifugal motor is located below the centrifugal turntable, the workbench is provided with an opening, the cross-sectional size of the opening is adapted to the centrifugal chamber cover, and the centrifugal chamber cover is located in the opening.

[0009] Furthermore, the centrifugal test tube rack includes a hanging basket rack, a hanging basket middle ring and a hanging basket fixed outer ring, the hanging basket fixed outer ring is evenly arranged around the centrifugal turntable and fixedly connected to the centrifugal turntable, the hanging basket fixed outer rings are in conflict with each other, the hanging basket middle ring is located on the inner side of the hanging basket fixed outer ring and fixedly connected to the hanging basket fixed outer ring, the hanging basket rack is located on the inner side of the hanging basket middle ring and fixedly connected to the hanging basket middle ring, the hanging basket rack is provided with a plurality of mounting holes, and the centrifuge mechanism also includes a centrifuge tube, and the centrifuge tube is located in the mounting hole.

[0010] Furthermore, the centrifugal test tube rack also includes a snap fixing plate and a hanging basket cover, the vibration rod is located at the center of the hanging basket rack, the snap fixing plate is located above the vibration rod and is fixedly connected to the vibration rod, the hanging basket cover is provided with a plurality of holes, the holes are consistent in size with the mounting holes, and the hanging basket cover is used to compress the centrifuge tube.

[0011] Furthermore, the oscillation mechanism also includes an oscillation lifting assembly, which includes a first upper connecting plate, an oscillation motor and an oscillation lifting electric cylinder. The oscillation motor and the eccentric ball seat are respectively fixed on the first upper connecting plate, and the oscillation lifting electric cylinder is located below the first upper connecting plate.

[0012] Furthermore, the oscillation lifting assembly also includes a second vertical plate, a second slider adapter plate, a second slide rail and a second slider, the second vertical plate is fixedly connected to the frame, the second slide rail is fixed on the second vertical plate, the second slider connecting plate is fixedly connected to the upper connecting plate, the second slider is fixed on the second slider connecting plate, and the second slide rail is slidably connected to the second slider.

[0013] Furthermore, the number of the second sliding blocks and the number of the second sliding rails are two respectively.

[0014] Further, the oscillation mechanism also includes a baffle assembly, which is located on the left side of the centrifugal mechanism. The baffle assembly includes a baffle, a first connecting plate, a second connecting plate and a baffle electric cylinder. The baffle is located above the centrifugal test tube rack, and the baffle is fixedly connected to the centrifugal chamber body. The plane where the baffle is located is parallel to the plane where the workbench is located. The first connecting plate is fixedly connected to the baffle, the second connecting plate is fixedly connected to the first connecting plate, and the baffle electric cylinder is fixed on the second connecting plate.

[0015] Furthermore, the fully automatic cell harvester also includes a supporting mechanism, which is located in the frame, and the supporting mechanism includes a third vertical plate, a second upper connecting plate, a third slide rail and a third slider, a second lower support cup, a first mounting seat and a first support shaft, the third vertical plate is fixed to the frame, the second upper connecting plate is fixedly connected to the third vertical plate, the third slide rail is fixed to the third vertical plate, the third slider is fixed to the second upper connecting plate, the third slide rail is slidably connected to the third slider, the first support shaft is fixed to the second upper connecting plate through the first mounting seat, the second lower support cup is located at the upper end of the first support shaft and fixedly connected to the first support shaft, and the second lower support cup is located below the vibration rod.

[0016] Compared with the existing technology, the utility model fully automatic cell harvester has the following advantages:

[0017] (1) The fully automatic cell harvester of the present application, the liquid injection mechanism, the liquid suction mechanism, the centrifugal mechanism, the oscillation mechanism, and the support mechanism are located in a frame, the frame includes a crossbeam and a workbench, the liquid injection mechanism and the liquid suction mechanism are fixed on the crossbeam, and the centrifugal mechanism, the oscillation mechanism, and the support mechanism are fixed under the workbench, which can realize the coordinated work of multiple mechanisms, build an integrated working platform, and complete the cell chromosome collection work;

[0018] (2) The fully automatic cell harvester of the present application uses the first slide rail and the first slider to realize the movement of the injection needle group in the X direction, and realizes the movement of the injection needle group in the Y direction through the injection electric cylinder, the first injection linear module and the second injection linear module, and realizes the stable operation of the mechanism in all directions through two sets of sliding modules;

[0019] (3) The fully automatic cell harvester of the present application, the ball shaft is plugged into the eccentric ball seat, and the vibration rod drives the centrifugal test tube rack to oscillate through the rotation of the eccentric ball seat;

[0020] (4) The fully automatic cell harvester of the present application requires the centrifuge test tube rack to remain horizontal during the process of placing centrifuge tubes and injecting and aspirating liquid. Therefore, a support mechanism is developed. The filling position is supported by the tube rack using part of the function of the oscillation mechanism. The aspiration position and the manual addition of test tubes are driven by two sets of third sliders and third slide rails to raise and lower the two support brackets. When aspirating liquid and placing centrifuge tubes, the support bracket rises to support the corresponding centrifuge test tube rack. The support bracket is kept disengaged at other times to keep the centrifuge test tube rack horizontal. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the structure of the fully automatic cell harvester of the utility model;

[0022] Figure 2 for Figure 1 Schematic diagram of the injection mechanism and aspiration mechanism of the fully automatic cell harvester;

[0023] Figure 3 for Figure 2 A schematic diagram of the structure of the injection mechanism;

[0024] Figure 4 for Figure 2 A schematic diagram of the structure of the injection needle lifting assembly and the injection needle group;

[0025] Figure 5 This is an exploded view of the injection needle lifting assembly structure;

[0026] Figure 6 It is a schematic diagram of the structure of the injection needle group;

[0027] Figure 7 for Figure 6 Exploded diagram of the injection needle assembly structure;

[0028] Figure 8 This is a structural schematic diagram of the injection needle assembly from another perspective;

[0029] Fig. 9 It is a schematic diagram of the structure of the liquid aspiration needle lifting assembly;

[0030] Fig.10 This is the exploded diagram of the aspiration needle group structure;

[0031] Fig.11 for Figure 1 Structural schematic diagram of the centrifugal mechanism, oscillating mechanism and supporting mechanism;

[0032] Fig.12 for Fig.11 Schematic diagram of the centrifugal mechanism and oscillating mechanism structure;

[0033] Fig.13 for Fig.11 Exploded view of the centrifugal mechanism structure;

[0034] Fig.14 for Fig.13 Exploded view of the centrifugal test tube rack mechanism;

[0035] Fig.15 for Fig.11 Schematic diagram of the oscillation mechanism structure;

[0036] Fig.16 for Fig.15 Schematic diagram of the structure of the oscillating lifting component;

[0037] Fig.17 for Fig.16 Exploded view of the structure of the eccentric ball seat;

[0038] Fig.18 for Fig.11 Schematic diagram of the supporting mechanism structure;

[0039] Fig.19 for Fig.11 Exploded view of the support structure.

[0040] In the figure: 100, frame; 101, crossbeam; 102, workbench; 103, first support rod; 104, first vertical rod; 105, second vertical rod; 200, injection mechanism; 210, injection needle translation assembly; 211, guide rail fixing plate; 212, first vertical plate; 213, first slide rail; 214, horizontal driving member; 215, synchronous belt; 216, first optical coupler; 217, first baffle; 220, injection needle lifting assembly; 221, injection back plate; 222, injection lifting transmission plate; 223, injection electric cylinder; 224, injection mounting plate; 225, first slider adapter plate; 226, first slider; 227, injection first linear module; 228, injection second linear module; 230, injection needle assembly; 231 , injection connection block; 232, injection needle holder; 233, injection clamping block; 234, injection needle; 2341, straight needle; 2342, curved needle; 235, rotating motor; 236, first bracket; 237, second bracket; 238, third optical coupler; 239, third baffle; 300, aspiration mechanism; 310, aspiration needle translation assembly; 320, aspiration needle lifting assembly; 321, aspiration back plate; 322, aspiration first mounting block; 323, aspiration second mounting block; 324, aspiration electric cylinder; 325, aspiration first linear module; 326, aspiration second linear module; 327, fourth optical coupler; 328, fourth baffle; 330, aspiration needle assembly; 331, aspiration connection block; 332, aspiration needle holder; 333, aspiration clamp Tightening block; 334, aspiration needle; 400, centrifugal mechanism; 410, centrifugal test tube rack; 411, hanging basket fixed outer ring; 412, hanging basket middle ring; 413, hanging basket rack; 414, hanging basket upper cover; 415, vibration rod; 416, snap fixing plate; 417, upper connecting piece; 418, bearing cover plate; 419, ball shaft; 420, centrifugal chamber body; 421, centrifugal chamber upper cover; 422, centrifugal chamber movable upper cover; 423, centrifugal chamber baffle; 424, centrifugal chamber fixed ring; 430, centrifugal turntable; 431, centrifugal upper cover plate; 440, centrifugal tube; 450, centrifugal motor; 451, mounting frame; 500, oscillation mechanism; 510, oscillation lifting assembly; 511, first upper connecting plate; 512, second slider adapter plate ; 513, oscillation motor; 514, eccentric ball seat; 5141, first lower support cup; 5142, elastic member; 5143, connecting sleeve; 5144, shaft connecting plate; 5145, large bearing cover; 5146, bearing seat; 5147, deep groove ball bearing; 5148, shaft; 5149, small bearing cover; 515, second vertical plate; 516, second slide rail; 517, second slider; 518, oscillation lifting cylinder; 520, baffle assembly; 521, baffle; 522, first connecting plate; 523, second connecting plate; 524, baffle cylinder; 600, supporting mechanism; 601, second upper connecting plate; 602, third slider adapter plate; 603, third vertical plate; 604, third slide rail; 605, third slider;606, supporting electric cylinder; 607, second lower supporting cup; 608, first supporting shaft; 609, first mounting seat; 610, centrifuge tube detection piece; 611, detection rack; 612, third lower supporting cup; 613, second supporting shaft; 614, second mounting seat; 700, waste liquid tank; 800, cleaning tank. ; DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0042] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may be another intermediate component through which it is fixed. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may be another intermediate component at the same time. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be another intermediate component at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0043] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0044] Figures 1 to 19 The utility model is a fully automatic cell harvester, such as Figure 1 As shown, the fully automatic cell harvester includes a crossbeam 101 , a liquid injection mechanism 200 and a liquid suction mechanism 300 , and the liquid injection mechanism 200 and the liquid suction mechanism 300 are installed on the crossbeam 101 .

[0045] like Figure 2-3 As shown, the injection mechanism 200 includes an injection needle translation assembly 210, an injection needle lifting assembly 220 and an injection needle group 230. The injection needle translation assembly 210 includes a guide rail fixing plate 211, a first vertical plate 212, a first slide rail 213, a horizontal driving member 214, and a synchronous belt 215.

[0046] Specifically, the horizontal driving member 214 is a linear motor. In some other embodiments, the horizontal driving member 214 is any motion mechanism that enables the injection mechanism 200 to move horizontally.

[0047] The guide rail fixing plate 211 and the first vertical plate 212 are fixed on the cross beam 101 , and the guide rail fixing plate 211 and the first vertical plate 212 are respectively fixed with first slide rails 213 .

[0048] Specifically, the plane where the guide rail fixing plate 211 is located is perpendicular to the plane where the first vertical plate 212 is located.

[0049] Specifically, there are multiple first slide rails 213 .

[0050] Specifically, the number of the first slide rails 213 is two.

[0051] The horizontal driving member 214 is fixedly mounted on the guide rail fixing plate 211 . The horizontal driving member 214 drives the synchronous belt 215 through the driving wheel, so that the injection mechanism 200 moves horizontally along the length direction of the beam 101 through the injection needle translation assembly 210 . The first slider adapter plate 225 is fixedly connected to the synchronous belt 215 .

[0052] The injection needle translation assembly 210 also includes a first optical coupler 216 and a first baffle 217. The first optical coupler 216 is located on the guide rail fixing plate 211, and the first baffle 217 is fixed on the first slider adapter plate 225. When the first baffle 217 is located in the first optical coupler 216, the injection needle translation assembly 210 is located in the initial position.

[0053] like Figure 4-5 As shown, the injection needle lifting assembly 220 includes an injection back plate 221, an injection lifting transmission plate 222, an injection electric cylinder 223, an injection mounting plate 224, a first slider adapter plate 225, a first slider 226, an injection first linear module 227, and an injection second linear module 228. The injection back plate 221 is fixedly connected to the first slider adapter plate 225, and the injection back plate 221 is fixedly connected to the injection lifting transmission plate 222.

[0054] The first slider adapter plate 225 is T-shaped, and first sliders 226 are fixed to both ends of the first slider adapter plate 225 , respectively. The first slider 226 is slidably connected to the first slide rail 213 .

[0055] Specifically, there are multiple first sliding blocks 226 .

[0056] Preferably, the number of the first sliding blocks 226 is two.

[0057] By providing two groups of sliding members, and the planes where the two groups of sliding members are located are perpendicular to each other, the movement of the injection mechanism 200 in the horizontal direction is made more stable and reliable.

[0058] Specifically, the liquid injection back plate 221 and the liquid injection lifting transmission plate 222 are arranged opposite to each other, and the liquid injection electric cylinder 223 is located between the liquid injection back plate 221 and the liquid injection lifting transmission plate 222 .

[0059] The liquid injection mounting plate 224 is fixedly connected to the liquid injection lifting transmission plate 222 . The liquid injection mounting plate 224 is L-shaped. A first liquid injection linear module 227 and a second liquid injection linear module 228 are fixed on the liquid injection mounting plate 224 .

[0060] Specifically, the plane where the first liquid injection linear module 227 is located and the plane where the second liquid injection linear module 228 is located are perpendicular to each other.

[0061] Specifically, the first injection linear module 227 and the second injection linear module 228 are slidably connected to the injection electric cylinder 223 respectively.

[0062] By providing two groups of linear modules, and the planes where the two groups of linear modules are located are perpendicular to each other, the vertical movement of the injection mechanism 200 is made more stable and reliable.

[0063] The injection needle lifting assembly 220 also includes a second optical coupler and a second baffle. The second optical coupler is installed on the injection back plate 221, and the second baffle is installed on the injection lifting transmission plate 222.

[0064] Specifically, the second optical coupler is a zero-point optical coupler, which is used to determine the initial position.

[0065] like Figure 6 to Figure 8 As shown, the injection needle group 230 also includes an injection connecting block 231, an injection needle holder 232, an injection clamping block 233 and an injection needle 234. The injection connecting block 231 is located below the second bracket 237 and is fixedly connected to the second bracket 237. The injection needle holder 232 is located below the injection connecting block 231 and is fixedly connected to the injection connecting block 231. The injection needle 234 is connected to the injection needle holder 232 via the injection clamping block 233.

[0066] Specifically, the injection needle holder 232 is cross-shaped and can fix at least two injection needles 234 in four directions. The injection needle holder 232 includes a groove and an opening, and the injection clamping block 233 has another groove adapted to the groove. The injection clamping block 233 is provided with an opening corresponding to the injection needle holder 232. The injection needle 234 passes through the groove and is clamped with the injection needle holder 232. The bolt passes through the corresponding opening to further tighten the injection needle 234.

[0067] The injection needle 234 includes a straight needle 2341 and a curved needle 2342 , and the curved needle 2342 is located inside the straight needle 2341 .

[0068] Specifically, the length of the curved needle 2342 is shorter than the length of the straight needle 2341 .

[0069] Specifically, there are multiple straight needles 2341.

[0070] Preferably, the number of straight needles 2341 is 4

[0071] Specifically, there are multiple bending needles 2342.

[0072] Preferably, the number of curved needles 2342 is equal to the number of straight needles 2341 .

[0073] Specifically, the injection needle 234 can be rotated 45°.

[0074] In a preferred embodiment, the ends of the curved needle 2342 and the straight needle 2341 are half-open, so that when injecting liquid, the added solution flows out along the end of the injection needle 234 and flows into the test tube through the test tube wall, thereby avoiding liquid splashing and secondary contamination.

[0075] In a preferred embodiment, the distance between the curved needle 2342 and the straight needle 2341 is smaller than the diameter of the test tube, so that the filling of the two liquids can be completed in one action, which simplifies the work process and improves work efficiency.

[0076] The injection needle group 230 includes a rotating motor 235, a first bracket 236, a coupling and a second bracket 237. The first bracket 236 is located below the injection lifting transmission plate 222. The first bracket 236 is fixedly connected to the injection lifting transmission plate 222. The rotating motor 235 is located in the first bracket 236. The second bracket 237 is located below the first bracket 236 and is fixedly connected to the first bracket 236. The coupling is located in the second bracket 237.

[0077] The injection needle assembly 230 further includes a third optical coupler 238 and a third baffle 239 . The third optical coupler 238 is fixed on the second bracket 237 , and the third baffle 239 is fixed on the injection clamping block 233 .

[0078] Specifically, the third optical coupler 238 is a zero-point optical coupler, which is used to determine the initial position.

[0079] The aspiration mechanism 300 includes a aspiration needle translation assembly 310 , a aspiration needle lifting assembly 320 and a aspiration needle group 330 . The aspiration needle group 330 is located below the aspiration needle lifting assembly 320 , and the aspiration needle group 330 is fixedly connected to the aspiration needle lifting assembly 320 .

[0080] Specifically, the structure of the aspiration needle translation assembly 310 is the same as that of the injection needle translation assembly 210, and will not be described in detail here.

[0081] like Figure 9-10As shown, the liquid suction needle lifting assembly 320 includes a liquid suction back plate 321, a first liquid suction mounting block 322, a second liquid suction mounting block 323, a liquid suction electric cylinder 324, a first liquid suction linear module 325 and a second liquid suction linear module 326. The liquid suction electric cylinder 324 and the first liquid suction mounting block 322 are fixed on the liquid suction back plate 321, the second liquid suction mounting block 323 is fixed on the first liquid suction mounting block 322, and the first liquid suction linear module 325 and the second liquid suction linear module 326 are respectively fixed on the second liquid suction mounting block 323.

[0082] Specifically, the first liquid suction mounting block 322 and the second liquid suction mounting block 323 are distributed at 90 degrees.

[0083] Specifically, the plane where the first liquid aspiration linear module 325 is located and the plane where the second liquid aspiration linear module 326 is located are perpendicular to each other.

[0084] Specifically, the first liquid suction linear module 325 and the second liquid suction linear module 326 are slidably connected to the liquid suction electric cylinder 324 respectively.

[0085] By providing two groups of linear modules, and the planes where the two groups of linear modules are located are perpendicular to each other, the vertical movement of the liquid suction mechanism 300 is made more stable and reliable.

[0086] The pipette needle lifting assembly 320 also includes a fourth optical coupler 327 and a fourth baffle 328 . The fourth optical coupler 327 is located on the pipette back plate 321 , and the fourth baffle 328 is located on the first pipette mounting block 322 .

[0087] Specifically, the fourth optical coupler 327 is a zero-point optical coupler, which is used to determine the initial position.

[0088] The pipette needle group 330 includes a pipette connecting block 331, a pipette needle rack 332, a pipette clamping block 333 and a pipette needle 334. The pipette connecting block 331 is located below the first pipette mounting block 322, the pipette connecting block 331 is fixedly connected to the first pipette mounting block 322, the pipette needle rack 332 is located below the pipette connecting block 331, the pipette needle rack 332 is fixedly connected to the pipette connecting block 331, and the pipette needle 334 is connected to the pipette needle rack 332 via the pipette clamping block 333.

[0089] Specifically, the pipetting needle 334 is a straight needle.

[0090] Specifically, there are multiple pipetting needles 334 .

[0091] Preferably, the number of the pipetting needles 334 is 8.

[0092] Specifically, the pipette needles 334 are evenly distributed in a circular pattern along the pipette needle holder 332 .

[0093] Specifically, the pipette needle 334 can be rotated 45°.

[0094] like Figure 11 to Figure 14 As shown, the centrifugal mechanism 400 includes a centrifugal test tube rack 410, a centrifugal chamber body 420, a centrifugal turntable 430, and a centrifugal motor 450. The centrifugal test tube rack 410, the centrifugal turntable 430, and the centrifugal tube 440 are located in the centrifugal chamber body 420, and the centrifugal motor 450 is located below the centrifugal chamber body 420.

[0095] The centrifugal test tube rack 410 includes a hanging basket fixed outer ring 411, a hanging basket rack 413 and a hanging basket middle ring 412. The hanging basket middle ring 412 is located inside the hanging basket fixed outer ring 411, the hanging basket rack 413 is located inside the hanging basket middle ring 412, the hanging basket fixed outer ring 411 is fixedly connected to the centrifugal turntable 430, the hanging basket middle ring 412 is fixedly connected to the hanging basket fixed outer ring 411, and the hanging basket rack 413 is fixedly connected to the hanging basket middle ring 412.

[0096] Specifically, the hanging basket fixing outer rings 411 are evenly arranged along the circumference of the centrifugal turntable 430, and the number of the hanging basket fixing outer rings 411 is multiple.

[0097] Specifically, the hanging basket middle ring 412 is pin-connected to the hanging basket fixed outer ring 411 , and the hanging basket frame 413 is pin-connected to the hanging basket middle ring 412 .

[0098] Preferably, the number of the hanging basket fixing outer rings 411 is 8. Adjacent hanging basket fixing outer rings 411 contact each other to form a stable connection and avoid displacement during oscillation.

[0099] The centrifuge test tube rack 410 also includes a hanging basket cover 414, a vibration rod 415, a snap fixing plate 416, an upper connecting piece 417, a bearing cover plate 418 and a ball shaft 419. The hanging basket frame 413 is provided with a plurality of mounting holes, and the centrifuge tube 440 is vertically inserted into the mounting holes. The hanging basket cover 414 is located above the hanging basket frame 413 and is movably connected to the hanging basket frame 413, so as to tighten the position of the centrifuge tube 440 to prevent displacement during oscillation.

[0100] The vibration rod 415 is located in the middle of the hanging basket frame 413, and the snap fixing plate 416 is located above the vibration rod 415 to fix the position of the vibration rod 415. The ball shaft 419 is located in the middle of the upper connecting piece 417 and connected to the lower end of the vibration rod 415, and the bearing cover plate 418 is located above the upper connecting piece 417.

[0101] The centrifugal chamber body 420 includes a centrifugal chamber upper cover 421, a centrifugal chamber movable upper cover 422, a centrifugal chamber baffle 423, and a centrifugal chamber fixed ring 424. The centrifugal chamber movable upper cover 422 is located on the centrifugal chamber upper cover 421 and is used to open the cover and place the centrifuge tube 440. Multiple openings are provided on the side of the centrifugal chamber body 420, and the centrifugal chamber baffle 423 is used to cover the multiple openings.

[0102] Specifically, the centrifugal bin upper cover 421 and the workbench 102 are located on the same plane.

[0103] The centrifugal turntable 430 further includes a centrifugal upper cover plate 431 , which is used to fix the position of the hanging basket fixing outer ring 411 , so that a plurality of hanging basket fixing outer rings 411 are tightly connected.

[0104] The centrifugal motor 450 is fixed below the centrifugal turntable 430 via a mounting frame 451 .

[0105] like Figure 15 to Figure 17 As shown, the oscillation mechanism 500 includes an oscillation lifting assembly 510 and a baffle assembly 520 . The oscillation lifting assembly 510 is located at the lower left of the centrifugal mechanism 400 , and the baffle assembly 520 is located at one side of the centrifugal mechanism 400 .

[0106] The oscillation lifting assembly 510 includes a first upper connecting plate 511, a second slider adapter plate 512, an oscillation motor 513, an eccentric ball seat 514, a second vertical plate 515, a second slide rail 516, a second slider 517 and an oscillation lifting electric cylinder 518. The oscillation motor 513 and the eccentric ball seat 514 are fixed on the first upper connecting plate 511, the second vertical plate 515 is fixedly connected to the first vertical rod 104, the second slide rail 516 is fixedly connected to the second vertical plate 515, the second slider adapter plate 512 is fixedly connected to the first upper connecting plate 511, the second slider 517 is located on the second slider adapter plate 512, and the second slider 517 is slidably connected to the second slide rail 516.

[0107] Specifically, the number of the second sliding block 517 and the second sliding rail 516 is two.

[0108] The eccentric ball seat 514 includes, from top to bottom, a first lower cup 5141, an elastic member 5142, a connecting sleeve 5143, a rotating shaft connecting plate 5144, a large bearing cover 5145, a bearing seat 5146, a deep groove ball bearing 5147, a rotating shaft 5148 and a small bearing cover 5149. The first lower cup 5141 is located directly below the vibration rod 415. The ball shaft 419 at the end of the vibration rod 415 is plugged into the eccentric ball seat 514, and the plug interface is in a bowl shape. The ball shaft 419 at the end of the vibration rod 415 is inserted into the eccentric ball seat 514. Under the rotation of the eccentric ball seat 514, the vibration rod 415 drives the centrifugal test tube rack 410 to vibrate.

[0109] The baffle assembly 520 includes a baffle 521, a first connecting plate 522, a second connecting plate 523 and a baffle electric cylinder 524. The baffle 521 is located above the centrifugal test tube rack 410. The baffle 521 is fixedly connected to the centrifugal chamber fixing ring 424. The second connecting plate 523 is fixedly connected to the first support rod 103. The baffle electric cylinder 524 is fixed on the second connecting plate 523. When oscillating, the baffle 521 extends out to prevent liquid from splashing far away.

[0110] Specifically, the plane where the baffle 521 is located is parallel to the plane where the workbench 102 is located. The plane where the first connecting plate 522 is located is parallel to the plane where the second connecting plate 523 is located.

[0111] like Figure 18-19 As shown, the fully automatic cell harvester also includes a supporting mechanism 600, which includes a second upper connecting plate 601, a third slider adapter plate 602, a third vertical plate 603, a third slide rail 604, a third slider 605, a supporting electric cylinder 606, a second lower supporting cup 607, a first supporting shaft 608, a first mounting seat 609, and a centrifuge tube detection member 610. The third vertical plate 603 is fixed on the second vertical rod 105, the second upper connecting plate 601 is fixedly connected to the third vertical plate 603, the third slide rail 604 is fixed on the third vertical plate 603, the third slider 605 is fixed on the second upper connecting plate 601, the third slider 605 is slidably connected to the third slide rail 604, the first supporting shaft 608 is fixed to the second upper connecting plate 601 through the first mounting seat 609, the second lower supporting cup 607 is located at the upper end of the first supporting shaft 608 and is fixedly connected to the first supporting shaft 608, and the second lower supporting cup 607 is located below the vibration rod 415.

[0112] The centrifuge tube detection component 610 includes a detection frame 611, a third lower support cup 612, a second support shaft 613, and a second mounting seat 614. The third lower support cup 612 is located at the upper end of the second support shaft 613 and is fixedly connected to the second support shaft 613. The third lower support cup 612 is located below the vibration rod 415. The centrifuge tube detection component 610 is used to detect whether the centrifuge tube 440 is inserted.

[0113] The supporting mechanism 600 is used to maintain the level of the centrifuge test tube rack 410 during the process of placing the centrifuge tube 440 and injecting and aspirating liquid. The injection level is supported by the tube rack using part of the function of the oscillation mechanism 500. The aspiration level and the manual test tube adding level are driven by the third slider 605 and the third slide rail 604 to lift and lower the two support supports. When aspirating liquid and placing the centrifuge tube 440, the second lower support cup 607 and the third lower support cup 612 rise to support the corresponding centrifuge test tube rack 410, and they are kept disengaged at other times.

[0114] The fully automatic cell harvester also includes a flushing and aspiration needle mechanism, a waste liquid pool 700 is located on the left side of the workbench 102, and a cleaning pool 800 is located on the right side of the workbench 102. The aspiration needle group 330 on the aspiration mechanism 300 is used to flush and extract the supernatant of the cell suspension to avoid residue. The cleaning pool 800 of the flushing and aspiration needle mechanism is connected to the automated flushing pipeline; the starting end of the pipeline is connected to the pure water storage bin, and pure water is introduced into the cleaning pool 800 through a water pump and a multi-channel liquid distributor; the aspiration needle group 330 can extract the liquid in the cleaning pool 800 by moving, and discharge the extracted cleaning liquid into the waste liquid collection bin. In addition, the cleaning module is also used to clean the waste liquid pool 700, and its main purpose is to clean the waste liquid pool 700 after the equipment is turned off, so as to keep the instrument clean and avoid residual dirt.

[0115] Specifically, the waste liquid tank 700 and the cleaning tank 800 are located on the same plane.

[0116] Specifically, a flushing pipe branch with a switching valve is led out from the multi-way distributor of the water inlet pipe, and a waste liquid pool 700 is arranged at the end of the flushing pipe branch. The injection needle group 230 can discharge the residual liquid inside into the waste liquid pool 700 by moving. In the cleaning module, a drainage pipe is led out from the lower part of the waste liquid pool 700 and the cleaning pool 800 and finally connected to the waste liquid collection bin.

[0117] When the present application is in use, the synchronous belt 215 is driven by the horizontal driving member 214 to drive the first slider 226 to move horizontally along the first slide rail 213, so that the injection mechanism 200 is translated, and the injection electric cylinder 223 provides power, and the first injection linear module 227 and the second injection linear module 228 move vertically along the injection electric cylinder 223, so that the injection needle group 230 moves up and down to complete the injection of liquid (including but not limited to peripheral blood, bone marrow, and amniotic fluid); the aspiration needle translation assembly 310 of the aspiration mechanism 300 causes the aspiration needle group 330 to move horizontally, and the first aspiration linear module 325 and the second aspiration linear module 326 move vertically along the aspiration electric cylinder 324, so that the aspiration needle group 3 30 makes horizontal movement and up and down movement to complete the absorption of liquid. The centrifugal test tube rack 410 is provided with a hole array for plugging in the centrifugal tube 440. The centrifugal test tube rack 410 can perform centrifugal movement under the drive of its centrifugal motor 450; the vibration rod 415 is arranged in each centrifugal test tube rack 410, and the ball shaft 419 at the end of the vibration rod 415 is plugged with the plug interface of the eccentric ball seat 514 in the oscillation mechanism 500. The plug interface is bowl-shaped. The ball shaft 419 at the end of the vibration rod 415 is inserted into the eccentric ball seat 514. Under the rotation of the eccentric ball seat 514, the vibration rod 415 drives the centrifugal test tube rack 410 to vibrate. During the oscillation, a baffle 521 extends to prevent the liquid from splashing far away. The fully automatic cell harvester of the present application, the injection mechanism 200, the suction mechanism 300, the centrifugal mechanism 400, the oscillation mechanism 500, and the support mechanism 600 are located in the frame 100, and the frame 100 includes a crossbeam 101 and a workbench 102. The injection mechanism 200 and the suction mechanism 300 are fixed on the crossbeam 101, and the centrifugal mechanism 400, the oscillation mechanism 500 and the support mechanism 600 are fixed below the workbench 102, which can realize the coordinated work of multiple mechanisms, build an integrated working platform, and complete the cell chromosome collection work; the first slide rail 213 and the first slider 226 are used to realize the movement of the injection needle group 230 in the X direction, and the injection electric cylinder 223, the first injection linear module 227 and the second injection linear module 228 are used to realize the movement of the injection needle group 230 in the X direction. The movement of the injection needle group 230 in the Y direction is realized, and the stable operation of the mechanism in all directions is achieved through two sets of sliding modules; the ball shaft 419 is plugged into the eccentric ball seat 514, and the vibration rod 415 drives the centrifugal test tube rack 410 to oscillate through the rotation of the eccentric ball seat 514; the centrifugal test tube rack 410 needs to remain horizontal during the process of placing the centrifugal tube 440 and injecting and aspirating liquid, so the support mechanism 600 is developed, and the injection position is partially supported by the function of the oscillation mechanism 500 to realize the tube rack support, and the two sets of third sliders 605 and the third slide rails 604 are used to drive the two support brackets to rise and fall at the liquid aspiration position and the manual test tube adding position. When aspirating liquid and placing the centrifugal tube 440, the support bracket rises to support the corresponding centrifugal test tube rack 410, and is usually kept in a disengaged state so that the centrifugal test tube rack 410 remains horizontal.

[0118] The above embodiments only express several implementation methods of the utility model, and the descriptions are relatively specific and detailed, but they cannot be understood as limiting the scope of the utility model patent. It should be pointed out that for ordinary technicians in this field, without departing from the concept of the utility model, several modifications and improvements can be made, which are equivalent modifications and improvements made to the above embodiments based on the essential technology of the utility model, and all of them belong to the protection scope of the utility model.

Claims

1. A fully automatic cell harvester, comprising a frame, characterized in that: It also includes an injection mechanism, a liquid suction mechanism, a centrifugal mechanism and an oscillating mechanism, wherein the injection mechanism, the liquid suction mechanism, the centrifugal mechanism and the oscillating mechanism are located in the frame, the frame includes a crossbeam and a workbench, the crossbeam is located above the workbench, the injection mechanism and the liquid suction mechanism are fixed on the crossbeam, the centrifugal mechanism is fixed on the workbench and located below the workbench, the injection mechanism includes an injection needle translation assembly, an injection needle lifting assembly and an injection needle group, the injection needle translation assembly and the injection needle lifting assembly move horizontally on the crossbeam, the injection needle lifting assembly moves vertically relative to the injection needle translation assembly, the oscillating mechanism is located below the workbench, the centrifugal mechanism includes a plurality of centrifugal test tube racks, the centrifugal test tube racks include a vibration rod and a ball shaft, the ball shaft is located below the vibration rod, the oscillating mechanism includes an eccentric ball seat, the vibration rod is plugged into the eccentric ball seat through the ball shaft, and the vibration rod drives the centrifugal test tube rack to oscillate through the rotation of the eccentric ball seat.

2. The fully automatic cell harvester according to claim 1, characterized in that: The injection needle translation assembly includes a guide rail fixing plate, a first vertical plate, a first slide rail and a first slider, the guide rail fixing plate and the first vertical plate are respectively fixed on the cross beam, the guide rail fixing plate and the first vertical plate are respectively fixed with the first slide rail, the injection needle lifting assembly includes a first slider adapter plate, the first sliders are respectively fixed at both ends of the first slider adapter plate, the first slide rail is slidably connected to the first slider, the injection needle lifting assembly also includes an injection electric cylinder and an injection linear module, the injection linear module is slidably connected to the injection electric cylinder.

3. The fully automatic cell harvester according to claim 1, characterized in that: The centrifugal mechanism includes a centrifugal chamber body, a centrifugal chamber upper cover, a centrifugal turntable and a centrifugal motor. The centrifugal test tube rack and the centrifugal turntable are located in the centrifugal chamber body. The centrifugal test tube rack is distributed along the circumference of the centrifugal turntable. The centrifugal motor is located below the centrifugal turntable. The workbench is provided with an opening, the cross-sectional size of the opening is adapted to the centrifugal chamber upper cover, and the centrifugal chamber upper cover is located in the opening.

4. The fully automatic cell harvester according to claim 3, characterized in that: The centrifugal test tube rack includes a hanging basket rack, a hanging basket middle ring and a hanging basket fixed outer ring, the hanging basket fixed outer ring is evenly arranged around the centrifugal turntable and fixedly connected to the centrifugal turntable, the hanging basket fixed outer rings are in conflict with each other, the hanging basket middle ring is located on the inner side of the hanging basket fixed outer ring and fixedly connected to the hanging basket fixed outer ring, the hanging basket rack is located on the inner side of the hanging basket middle ring and fixedly connected to the hanging basket middle ring, the hanging basket rack is provided with a plurality of mounting holes, the centrifugal mechanism also includes a centrifuge tube, and the centrifuge tube is located in the mounting hole.

5. The fully automatic cell harvester according to claim 4, characterized in that: The centrifugal test tube rack also includes a snap fixing plate and a hanging basket cover, the vibration rod is located at the center of the hanging basket rack, the snap fixing plate is located above the vibration rod and is fixedly connected to the vibration rod, the hanging basket cover is provided with a plurality of holes, the holes are consistent in size with the mounting holes, and the hanging basket cover is used to compress the centrifuge tube.

6. The fully automatic cell harvester according to claim 5, characterized in that: The oscillation mechanism also includes an oscillation lifting component, which includes a first upper connecting plate, an oscillation motor and an oscillation lifting electric cylinder. The oscillation motor and the eccentric ball seat are respectively fixed on the first upper connecting plate, and the oscillation lifting electric cylinder is located below the first upper connecting plate.

7. The fully automatic cell harvester according to claim 6, characterized in that: The oscillating lifting assembly also includes a second vertical plate, a second slider adapter plate, a second slide rail and a second slider, the second vertical plate is fixedly connected to the frame, the second slide rail is fixed on the second vertical plate, the second slider adapter plate is fixedly connected to the upper connecting plate, the second slider is fixed on the second slider adapter plate, and the second slide rail is slidably connected to the second slider.

8. The fully automatic cell harvester according to claim 7, characterized in that: The number of the second sliding blocks and the number of the second sliding rails are two respectively.

9. The fully automatic cell harvester according to claim 7, characterized in that: The oscillation mechanism also includes a baffle assembly, which is located on the left side of the centrifugal mechanism. The baffle assembly includes a baffle, a first connecting plate, a second connecting plate and a baffle electric cylinder. The baffle is located above the centrifugal test tube rack, and the baffle is fixedly connected to the centrifugal chamber body. The plane where the baffle is located is parallel to the plane where the workbench is located. The first connecting plate is fixedly connected to the baffle, the second connecting plate is fixedly connected to the first connecting plate, and the baffle electric cylinder is fixed on the second connecting plate.

10. The fully automatic cell harvester according to claim 6, characterized in that: The fully automatic cell harvester also includes a supporting mechanism, which is located in the frame. The supporting mechanism includes a third vertical plate, a second upper connecting plate, a third slide rail and a third slider, a second lower supporting cup, a first mounting seat and a first supporting shaft. The third vertical plate is fixed to the frame, the second upper connecting plate is fixedly connected to the third vertical plate, the third slide rail is fixed to the third vertical plate, the third slider is fixed to the second upper connecting plate, the third slide rail is slidably connected to the third slider, the first supporting shaft is fixed to the second upper connecting plate through the first mounting seat, the second lower supporting cup is located at the upper end of the first supporting shaft and is fixedly connected to the first supporting shaft, and the second lower supporting cup is located below the vibration rod.

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