Adsorption and replacement device for plant signal protein production

By designing an adsorption replacement device including multiple solvent tubes, feed tubes and electric valves, the problem that existing devices cannot accurately control solvent filling and mixing is solved, and efficient protein adsorption and desorption are achieved, which significantly improves the efficiency and accuracy of the device.

CN222566872UActive Publication Date: 2025-03-07QIANJIANG AGRI MICROBIOLOGY ENG CO LTD
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Patent Information

Application Number
CN202421295867.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-06-07
Publication Date
2025-03-07
Estimated Expiration
2034-06-07

AI Technical Summary

Technical Problem

The existing adsorption replacement device for plant signal protein production cannot accurately control the amount and concentration of the infused solvent, resulting in low adsorption and desorption efficiency of proteins, and uneven mixing of exchangers affects the adsorption and elution process of proteins.

Method used

An adsorption replacement device including two solvent tubes, two feed tubes and electric valves is designed. By controlling the opening and closing of the electric valve, the flow and mixing of the solvent is accurately controlled, and the solvent level is controlled by using float balls and contact sensors to ensure that the solvent is fully mixed and precisely filled.

Benefits of technology

Accurate control of solvents is achieved, the adsorption and desorption efficiency of proteins is improved, and the uniform mixing of exchangers is ensured, which significantly improves the efficiency and accuracy of the device.

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Abstract

The utility model relates to the technical field of plant signal protein production, and discloses an adsorption replacement device for plant signal protein production, which comprises a barrel body, a discharge pipe and a support are mounted on the outer side surface of the barrel body, a solvent pipe is mounted on the top surface of the support, a first motor is mounted on the top surface of the solvent pipe, and a threaded rod and a moving block are mounted in the solvent pipe. A floating ball is slidably mounted on the outer side face of the threaded rod, a contact sensor is mounted on the bottom face of the moving block, a first feeding pipe and a second feeding pipe are mounted on the outer side face of the solvent pipe, and a funnel is connected to the end, away from the solvent pipe, of the second feeding pipe. According to the solvent filling device, the first motor is started, the output shaft of the first motor can drive the threaded rod to rotate, and the threaded rod can drive the movable block to move in the vertical direction, so that the height position of the contact sensor can be effectively adjusted, and then the solvent filling quantity can be adjusted according to different conditions; and the overall practicability of the device is improved.
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Description

Technical Field

[0001] The utility model relates to the technical field of plant signal protein production, in particular to an adsorption and replacement device for plant signal protein production. Background Art

[0002] Plant signaling proteins are a class of natural proteins that can be recognized by plant surface cell receptors. They can activate signal transduction in plants, thereby stimulating plant growth and defense capabilities. In the process of plant protein extraction and purification, a variety of biomacromolecules are usually involved, including proteins. These proteins may undergo conformational changes during the extraction process. Therefore, an adsorption displacement device is needed to separate the target protein from the mixture and remove impurities by physical or chemical means, thereby achieving precise control of product quality.

[0003] According to the search, Chinese patent literature, announcement number: CN216946821U, discloses an adsorption displacement device for the production of plant immune proteins. The solution accumulates inside the feed chamber and the weight gradually increases. The gravity generated by this weight will be transmitted to the double-sided rack through the connecting rod and the first gear. At the same time, the electromagnet generates suction to adsorb the double-sided rack. When the gravity is greater than the force of the electromagnet, the double-sided rack moves upward, so that the first gear, the connecting rod and the sealing plate will rotate, causing the solution to leak out. However, the device cannot accurately control the amount and concentration of the added solvent during actual use, so it will directly affect the adsorption and desorption efficiency of the protein; in addition, when the device is filled with the exchange agent, the exchange agent is directly injected into the interior of the shell to mix with the solution, which will cause uneven mixing, thereby affecting the adsorption and elution process of the protein. Utility Model Content

[0004] 1. Technical issues to be solved

[0005] In view of the deficiencies in the prior art, the utility model provides an adsorption and displacement device for the production of plant signal proteins, which has the advantages of accurately controlling the amount of added solvent, allowing the solvent to be fully and effectively mixed, and improving the production efficiency of the device, thereby solving the above-mentioned technical problems.

[0006] (II) Technical solution

[0007] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an adsorption and displacement device for the production of plant signal proteins, comprising a barrel body, a discharge pipe and a bracket are fixedly installed on the outer side surface of the barrel body, a solvent tube is fixedly installed on the top surface of the bracket, a first motor is fixedly installed on the top surface of the solvent tube, a threaded rod and a moving block are installed inside the solvent tube, a float is slidably installed on the outer side surface of the threaded rod, a contact sensor is fixedly installed on the bottom surface of the moving block, a first feed pipe and a second feed pipe are fixedly installed on the outer side surface of the solvent tube, a funnel is connected to the end of the second feed pipe away from the solvent tube, a second motor is fixedly installed on the top surface of the barrel body, an output shaft of the second motor is fixedly connected to a rotating shaft, and a spiral blade is fixedly installed on the outer side surface of the rotating shaft.

[0008] Preferably, the number of the bracket, the solvent tube, the first feed tube and the second feed tube are all two.

[0009] Through the above technical solution, different solvents can be input into the interior of the two solvent tubes through the two first feed pipes. After that, when the solvent is injected to a certain amount, it can be input into the interior of the funnel through the two second feed pipes. The solvent can then be preliminarily mixed inside the funnel, thereby improving the overall practicality of the device.

[0010] Preferably, electric valves are installed on the outer sides of the first feed pipe and the second feed pipe.

[0011] Through the above technical solution, the flow of solvent can be effectively controlled by controlling the opening and closing of multiple electric valves, thereby realizing the addition of solvent, control of solvent quantity, control of filling time, etc., which not only reduces the need for manual operation and reduces the labor intensity of staff, but also can significantly improve the efficiency and accuracy of the device.

[0012] Preferably, the first motor, the threaded rod, the moving block, the float and the contact sensor are each provided with two, and the output shaft of the first motor is fixedly connected to the top end of the threaded rod, and the moving block is threadedly connected to the outer surface of the threaded rod.

[0013] Through the above technical solution, when the solvent is added to the interior of the solvent tube, as the solvent liquid level continues to rise, the float will also be driven to move upward. When the float touches the contact sensor, the electric valve inside the first feed tube can be controlled to close to stop the injection of solvent. Therefore, the liquid level and amount of the added solvent can be accurately controlled. In addition, when the first motor is started, the output shaft of the first motor can drive the threaded rod to rotate, and the threaded rod can then drive the moving block to move in the vertical direction. Therefore, the height position of the contact sensor can be effectively adjusted, and the amount of solvent added can be adjusted according to different situations, thereby improving the overall practicality and convenience of the device.

[0014] Preferably, a limiting slide rail is provided inside the solvent tube, and the moving block is slidably installed inside the limiting slide rail.

[0015] Through the above technical solution, when the moving block moves inside the solvent tube, the limiting slide rail can effectively limit its position to prevent rotation and the like. In addition, the limiting slide rail can also limit the moving path of the moving block to avoid excessive movement, thereby improving the overall stability and ensuring the normal operation of the device.

[0016] Preferably, a plurality of connecting rods are fixedly mounted on the top surface of the funnel, the top ends of the connecting rods are fixedly connected to the inner top surface of the barrel body, and the rotating shaft and the spiral blades are both mounted inside the funnel.

[0017] Through the above technical solution, when different solvents are input into the interior of the funnel through the second feed pipe, the different solvents can be preliminarily mixed, and then the second motor is started. The output shaft of the second motor can drive the rotating shaft and the spiral blades to rotate together. The spiral blades can not only fully stir and mix the solvents, but also help promote effective contact between plant tissues and solvents, thereby improving the protein extraction efficiency.

[0018] Compared with the prior art, the utility model provides an adsorption and displacement device for producing plant signal proteins, which has the following beneficial effects:

[0019] 1. The utility model starts the first motor, and the output shaft of the first motor can drive the threaded rod to rotate, and the threaded rod can then drive the moving block to move in the vertical direction, so that the height position of the contact sensor can be effectively adjusted, and then the amount of solvent filling can be adjusted according to different situations, thereby improving the overall practicality and convenience of the device; and the limiting slide rail can effectively limit the position of the moving block to prevent self-rotation and the like. In addition, the limiting slide rail can also limit the moving path of the moving block to avoid excessive movement, thereby improving the overall stability and ensuring the normal operation of the device; then, different solvents can be input into the interior of the two solvent tubes through the two first feed pipes, and as the solvent liquid level continues to rise, it will also drive the float to move upward together. When the float touches the contact sensor, the electric valve inside the first feed pipe can be controlled to close to stop the continued injection of solvent, thereby accurately controlling the liquid level height and amount of the added solvent.

[0020] 2. The utility model can effectively control the flow of solvent by controlling the opening and closing of multiple electric valves, thereby realizing the filling of solvent, controlling the amount of solvent, controlling the filling time, etc., which not only reduces the need for manual operation and reduces the labor intensity of staff, but also can significantly improve the efficiency and accuracy of the device; when different solvents are input into the interior of the funnel through the second feed pipe, different solvents can be preliminarily mixed, and then the second motor is started. The output shaft of the second motor can drive the rotating shaft and the spiral blades to rotate together. The spiral blades can not only fully stir and mix the solvent, but also help promote effective contact between plant tissue and solvent, thereby improving the extraction efficiency of protein. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a three-dimensional schematic diagram of the structure of the utility model;

[0022] Figure 2 It is a front cross-sectional schematic diagram of the barrel and other parts of the utility model structure;

[0023] Figure 3 It is a three-dimensional cross-sectional schematic diagram of the solvent tube and other parts of the utility model structure.

[0024] Among them: 1. barrel body; 2. discharge pipe; 3. bracket; 4. solvent pipe; 5. first motor; 6. threaded rod; 7. moving block; 8. float; 9. contact sensor; 10. first feed pipe; 11. second feed pipe; 12. funnel; 13. second motor; 14. rotating shaft; 15. spiral blade; 16. electric valve; 17. limit slide rail; 18. connecting rod. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] See also Figure 1-3, an adsorption and displacement device for producing plant signal proteins, comprising a barrel body 1, a discharge pipe 2 and a bracket 3 are fixedly installed on the outer side of the barrel body 1, a solvent tube 4 is fixedly installed on the top surface of the bracket 3, a first motor 5 is fixedly installed on the top surface of the solvent tube 4, a threaded rod 6 and a moving block 7 are installed inside the solvent tube 4, a float 8 is slidably installed on the outer side of the threaded rod 6, a contact sensor 9 is fixedly installed on the bottom surface of the moving block 7, a first feed pipe 10 and a second feed pipe 11 are fixedly installed on the outer side of the solvent tube 4, a funnel 12 is connected to the end of the second feed pipe 11 away from the solvent tube 4, a second motor 13 is fixedly installed on the top surface of the barrel body 1, an output shaft of the second motor 13 is fixedly connected to a rotating shaft 14, and a spiral blade 15 is fixedly installed on the outer side of the rotating shaft 14.

[0027] Specifically, the number of the bracket 3, the solvent tube 4, the first feed tube 10 and the second feed tube 11 are all two. The advantage is that different solvents can be input into the two solvent tubes 4 through the two first feed tubes 10, and then when the solvents are injected to a certain amount, they can be input into the funnel 12 through the two second feed tubes 11, and the solvents can be preliminarily mixed in the funnel 12, thereby improving the overall practicality of the device.

[0028] Specifically, the outer side of the first feed pipe 10 and the second feed pipe 11 are both installed with electric valves 16. The advantage is that the flow of the solvent can be effectively controlled by controlling the opening and closing of multiple electric valves 16, thereby realizing the filling of the solvent, controlling the amount of solvent, controlling the filling time, etc., which not only reduces the need for manual operation and the labor intensity of the staff, but also can significantly improve the efficiency and accuracy of the device.

[0029] Specifically, the number of the first motor 5, the threaded rod 6, the moving block 7, the floating ball 8 and the contact sensor 9 is set to two, and the output shaft of the first motor 5 is fixedly connected to the top of the threaded rod 6, and the moving block 7 is threadedly connected to the outer surface of the threaded rod 6. The advantage is that through this structure, when the solvent is added to the inside of the solvent tube 4, as the solvent liquid level continues to rise, it will also drive the floating ball 8 to move upward together. When the floating ball 8 touches the contact sensor 9, the electric valve 16 inside the first feeding pipe 10 can be controlled to close to stop the continued injection of solvent, so the liquid level height and amount of the added solvent can be accurately controlled; in addition, when the first motor 5 is started, the output shaft of the first motor 5 can drive the threaded rod 6 to rotate, and the threaded rod 6 can then drive the moving block 7 to move in the vertical direction, so the height position of the contact sensor 9 can be effectively adjusted, and then the amount of solvent filling can be adjusted according to different situations, thereby improving the overall practicality and convenience of the device.

[0030] Specifically, a limit slide rail 17 is provided inside the solvent tube 4, and the moving block 7 is slidably installed inside the limit slide rail 17. The advantage is that, through this structure, when the moving block 7 moves inside the solvent tube 4, the limit slide rail 17 can effectively limit its position to prevent rotation and the like, and the limit slide rail 17 can also limit the moving path of the moving block 7 to prevent it from moving too much, thereby improving the overall stability and ensuring the normal operation of the device.

[0031] Specifically, a plurality of connecting rods 18 are fixedly mounted on the top surface of the funnel 12, the top ends of the connecting rods 18 are fixedly connected to the inner top surface of the barrel body 1, and the rotating shaft 14 and the spiral blades 15 are both mounted inside the funnel 12. The advantage is that when different solvents are input into the interior of the funnel 12 through the second feeding pipe 11, the different solvents can be preliminarily mixed, and then the second motor 13 is started, and the output shaft of the second motor 13 can drive the rotating shaft 14 and the spiral blades 15 to rotate together, and the spiral blades 15 can not only fully stir and mix the solvent, but also help promote effective contact between plant tissue and the solvent, thereby improving the extraction efficiency of protein.

[0032] During use, by starting the first motor 5, the output shaft of the first motor 5 can drive the threaded rod 6 to rotate, and the threaded rod 6 can then drive the moving block 7 to move in the vertical direction, so that the height position of the contact sensor 9 can be effectively adjusted, and then the amount of solvent filling can be adjusted according to different situations, thereby improving the overall practicality and convenience of the device; and the limiting slide rail 17 can effectively limit the position of the moving block 7 to prevent self-rotation and the like. In addition, the limiting slide rail 17 can also limit the movement path of the moving block 7 to avoid excessive movement, thereby improving the overall stability and ensuring the normal operation of the device; thereafter, different solvents can be input into the interior of the two solvent tubes 4 through the two first feeding pipes 10, and as the solvent liquid level continues to rise, it will also drive the float 8 to move upward together, and when the float 8 touches the contact sensor 9, the first The electric valve 16 inside the feed pipe 10 stops the injection of solvent, so the liquid level and amount of the added solvent can be accurately controlled; the flow of the solvent can be effectively controlled by controlling the opening and closing of multiple electric valves 16, so that the solvent can be added, the amount of solvent can be controlled, the filling time can be controlled, etc., which not only reduces the need for manual operation and reduces the labor intensity of the staff, but also can significantly improve the efficiency and accuracy of the device; when different solvents are input into the funnel 12 through the second feed pipe 11, the different solvents can be preliminarily mixed, and then the second motor 13 is started. The output shaft of the second motor 13 can drive the rotating shaft 14 and the spiral blade 15 to rotate together. The spiral blade 15 can not only fully stir and mix the solvent, but also help to promote effective contact between plant tissue and solvent, thereby improving the extraction efficiency of protein.

[0033] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An adsorption and displacement device for producing plant signal proteins, comprising a barrel (1), characterized in that: A discharge pipe (2) and a bracket (3) are fixedly mounted on the outer side surface of the barrel body (1); a solvent pipe (4) is fixedly mounted on the top surface of the bracket (3); a first motor (5) is fixedly mounted on the top surface of the solvent pipe (4); a threaded rod (6) and a moving block (7) are mounted inside the solvent pipe (4); a floating ball (8) is slidably mounted on the outer side surface of the threaded rod (6); a contact sensor (9) is fixedly mounted on the bottom surface of the moving block (7); a first feed pipe (10) and a second feed pipe (11) are fixedly mounted on the outer side surface of the solvent pipe (4); a funnel (12) is connected to the end of the second feed pipe (11) away from the solvent pipe (4); a second motor (13) is fixedly mounted on the top surface of the barrel body (1); an output shaft of the second motor (13) is fixedly connected to a rotating shaft (14); a spiral blade (15) is fixedly mounted on the outer side surface of the rotating shaft (14).

2. The adsorption and displacement device for producing plant signal proteins according to claim 1, characterized in that: The number of the bracket (3), the solvent tube (4), the first feed tube (10) and the second feed tube (11) is two.

3. The adsorption and displacement device for producing plant signal proteins according to claim 1, characterized in that: Electric valves (16) are installed on the outer sides of the first feeding pipe (10) and the second feeding pipe (11).

4. The adsorption and displacement device for producing plant signal proteins according to claim 1, characterized in that: The first motor (5), the threaded rod (6), the moving block (7), the float (8) and the contact sensor (9) are all provided in two numbers, and the output shaft of the first motor (5) is fixedly connected to the top end of the threaded rod (6), and the moving block (7) is threadedly connected to the outer surface of the threaded rod (6).

5. The adsorption and displacement device for producing plant signal protein according to claim 1, characterized in that: A limiting slide rail (17) is arranged inside the solvent tube (4), and the moving block (7) is slidably mounted inside the limiting slide rail (17).

6. The adsorption and displacement device for producing plant signal proteins according to claim 1, characterized in that: A plurality of connecting rods (18) are fixedly mounted on the top surface of the funnel (12), the top ends of the connecting rods (18) are fixedly connected to the inner top surface of the barrel body (1), and the rotating shaft (14) and the spiral blades (15) are both mounted inside the funnel (12).

Citation Information

Patent Citations

  • Adsorption replacement device for plant immune protein production

    CN216946821U