Parainfluenza virus detection kit
By designing a parainfluenza virus detection kit, using fluorescent PCR technology and internal reference control, the problem of early virus detection in the existing technology is solved, and a more accurate virus detection result is achieved.
Patent Information
- Application Number
- CN202421233171.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-31
- Publication Date
- 2025-03-07
- Estimated Expiration
- 2034-05-31
AI Technical Summary
The prior art is prone to false negative or false positive results in early virus detection, resulting in misjudgment.
A parainfluenza virus detection kit was designed, using fluorescent PCR technology and internal reference control, and qualitative detection was performed through the solution in the reagent tube to monitor the sample collection, storage and transportation process to avoid misjudgment of false negative results.
It effectively avoids misjudgment of false negative results, improves the accuracy of early virus detection, and ensures the reliability of judgment.
Smart Images
Figure CN222572938U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of parainfluenza virus test kits, in particular to a parainfluenza virus detection kit. Background Art
[0002] Parainfluenza virus (PIV) is an important pathogen of the respiratory tract of infants and young children, and is the second most common pathogen of pneumonia and bronchiolitis in infants under 6 months old. PIV can cause cold symptoms such as fever and sore throat, leading to recurrent lower respiratory tract diseases such as pneumonia, bronchitis and bronchiolitis, especially in infants, the elderly and people with immunodeficiency.
[0003] However, in the prior art, most of the diagnosis is made through molecular detection methods (such as PCR), but in some cases, false negative or false positive results may occur. Especially in the early stage of the disease or when the viral load is low, the sensitivity may be limited and it is easy to make mistakes. Therefore, in view of the above shortcomings, a parainfluenza virus detection kit is proposed. Utility Model Content
[0004] The purpose of the utility model is to solve the shortcomings of the prior art and to propose a parainfluenza virus detection kit, which aims to improve the problem that false negative or false positive results may occur in the prior art when detecting early viruses, resulting in easy misjudgment.
[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0006] A parainfluenza virus detection kit comprises a kit body, wherein the left and right ends of the top side of the kit body are fixedly connected to side plates, the rear end of the top side of the kit body is fixedly connected to a cover plate, the inner wall of the kit body is slidably connected to a top plate, the left and right ends of the bottom side of the top plate are fixedly connected to connecting plates, the outsides of the two connecting plates are fixedly connected to middle plates, the bottom sides of the two connecting plates are fixedly connected to bottom plates, the top plate, the middle plate and the bottom plate are fixedly connected to multiple circular holes, and the top side of the middle plate is provided with a separation component.
[0007] As a further description of the above technical solution:
[0008] The separation component includes two reagent tubes 1, the bottom sides of the two reagent tubes 1 are in contact with the left end of the top side of the middle plate, the middle part of the top side of the middle plate is in contact with two reagent tubes 2, the middle part of the top side of the middle plate is in contact with two reagent tubes 3, and the right end of the top side of the middle plate is in contact with a reagent tube 4.
[0009] As a further description of the above technical solution:
[0010] The outside of the middle plate is slidably connected to the inner wall of the reagent box body, and the outside of the bottom plate is slidably connected to the inner wall of the reagent box body.
[0011] As a further description of the above technical solution:
[0012] The reagent tube one, reagent tube two, reagent tube three, and reagent tube four are respectively slidably connected to the inner walls of two of the circular holes, the solution inside the reagent tube one is RT-PCR buffer, the solution inside the reagent tube two is RT-PCR reaction enzyme system, and the solution inside the reagent tube three is primer probe reaction solution.
[0013] The utility model has the following beneficial effects:
[0014] 1. In the present invention, qualitative detection is performed through the solutions inside the human parainfluenza virus reagent tube 1, reagent tube 2, and reagent tube 3, and an internal reference control is set using the kit to monitor the sample collection, storage and transportation and nucleic acid extraction process, thereby avoiding the misjudgment of false negative results.
[0015] 2. In the utility model, holes of different sizes are opened inside the top plate, the middle plate and the bottom plate to correspond to circular holes of different sizes for installation, so that reagent bottles of different sizes can be accommodated, thereby expanding the scope of use of the device. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 A three-dimensional diagram of a parainfluenza virus detection kit proposed by the utility model;
[0017] Figure 2 This is a schematic diagram of the top plate structure of a parainfluenza virus detection kit proposed by the utility model;
[0018] Figure 3 The present invention is a schematic diagram of the bottom plate structure of a parainfluenza virus detection kit proposed by the present invention.
[0019] Legend:
[0020] 1. Reagent box body; 2. Side plate; 3. Cover plate; 4. Top plate; 5. Connecting plate; 6. Middle plate; 7. Bottom plate; 8. Round hole; 9. Reagent tube one; 10. Reagent tube two; 11. Reagent tube three; 12. Reagent tube four. DETAILED DESCRIPTION
[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0022] Reference Figure 1 - Figure 2 , an embodiment provided by the utility model: a parainfluenza virus detection kit, comprising a kit body 1, the left and right ends of the top side of the kit body 1 are fixedly connected with side panels 2, the top rear end of the kit body 1 is fixedly connected with a cover plate 3, the kit body 1, the side panels 2, and the cover plate 3 are all processed by fluorescent PCR technology, the inner wall of the kit body 1 is slidably connected with a top plate 4, and the top plate 4 can slide on the inner wall of the kit body 1 and engage with it through the restriction of paper, the left and right ends of the bottom side of the top plate 4 are fixedly connected with connecting plates 5 to provide support for the connecting plates 5, the outer sides of the two connecting plates 5 are fixedly connected with middle plates 6 for limiting medicine bottles of different sizes, the bottom sides of the two connecting plates 5 are fixedly connected with bottom plates 7, the outer side of the middle plate 6 is slidably connected to the inner wall of the kit body 1, and the outer side of the bottom plate 7 is slidably connected to the inner wall of the kit body 1, and the inner sides of the top plate 4, the middle plate 6, and the bottom plate 7 are all provided with a plurality of circular holes 8 for further fixing and limiting the medicine bottles;
[0023] Reference Figure 1 and Figure 3 A separation component is provided on the top side of the middle plate 6, and the separation component includes two reagent tubes 9 for storage. The bottom sides of the two reagent tubes 9 are in contact with the left end of the top side of the middle plate 6 to provide support for the reagent tube 9. Two reagent tubes 10 are in contact with the middle part of the top side of the middle plate 6 for storage. Two reagent tubes 3 11 are in contact with the middle part of the top side of the middle plate 6 for storage. A reagent tube 4 12 is in contact with the right end of the top side of the middle plate 6. Reagent tube 1 9, reagent tube 2 10, reagent tube 3 11, and reagent tube 4 12 are respectively slidably connected to the inside of two circular holes 8 therein, so that reagent tubes 1 9, reagent tube 2 10, reagent tube 3 11, and reagent tube 4 12 of different thicknesses can be fixed. The solution inside the reagent tube 9 is RT-PCR buffer, the solution inside the reagent tube 2 10 is RT-PCR reaction enzyme system, the solution inside the reagent tube 3 11 is primer probe reaction solution, and the solution in the reagent tube 4 12 is negative control and positive control.
[0024] Working principle: We process the reagent kit body 1, side plate 2, and cover plate 3 by fluorescent PCR technology, and achieve qualitative analysis of the starting template by real-time detection of the fluorescent signal of each cycle product in the PCR amplification reaction. Then, by selecting the relatively conservative region HN of the solution inside the reagent tube 1 9, reagent tube 2 10, and reagent tube 3 11 as the amplification target region, and the human RNaseP housekeeping gene as the internal reference target sequence, specific primers and probes are designed, and amplified by a one-step real-time fluorescent PCR system, the solution inside the reagent tube 1 9, reagent tube 2 10, and reagent tube 3 11 infected with human parainfluenza virus is qualitatively detected, and the internal reference control is set up using the kit to monitor the sample collection, storage and transportation and nucleic acid extraction process, thereby avoiding the misjudgment of false negative results;
[0025] At the same time, holes of different sizes are opened inside the top plate 4, the middle plate 6 and the bottom plate 7 to respectively correspond to the circular holes 8 of different sizes for fitting and installation, so that reagent bottles of different sizes can be accommodated, thereby expanding the scope of use of the device.
[0026] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A parainfluenza virus detection kit, comprising a kit body (1), characterized in that: The left and right ends of the top side of the reagent box body (1) are fixedly connected to side plates (2), the rear end of the top side of the reagent box body (1) is fixedly connected to a cover plate (3), the inner wall of the reagent box body (1) is slidably connected to a top plate (4), the left and right ends of the bottom side of the top plate (4) are fixedly connected to connecting plates (5), the outsides of the two connecting plates (5) are fixedly connected to a middle plate (6), the bottom sides of the two connecting plates (5) are fixedly connected to a bottom plate (7), the top plate (4), the middle plate (6) and the bottom plate (7) are all provided with a plurality of circular holes (8), and a separation component is provided on the top side of the middle plate (6).
2. A parainfluenza virus detection kit according to claim 1, characterized in that: The separation assembly comprises two reagent tubes one (9), the bottom sides of the two reagent tubes one (9) are in contact with the left end of the top side of the middle plate (6), the middle part of the top side of the middle plate (6) is in contact with two reagent tubes two (10), the middle part of the top side of the middle plate (6) is in contact with two reagent tubes three (11), and the right end of the top side of the middle plate (6) is in contact with a reagent tube four (12).
3. A parainfluenza virus detection kit according to claim 1, characterized in that: The outside of the middle plate (6) is slidably connected to the inner wall of the reagent box body (1), and the outside of the bottom plate (7) is slidably connected to the inner wall of the reagent box body (1).
4. A parainfluenza virus detection kit according to claim 2, characterized in that: The reagent tube one (9), reagent tube two (10), reagent tube three (11), and reagent tube four (12) are respectively slidably connected to the inside of two of the circular holes (8); the solution inside the reagent tube one (9) is an RT-PCR buffer solution, the solution inside the reagent tube two (10) is an RT-PCR reaction enzyme system, and the solution inside the reagent tube three (11) is a primer probe reaction solution.