Pre-segmented fluorescent quantitative PCR (Polymerase Chain Reaction) plate sealing film

Abstract

The utility model discloses a pre-segmented fluorescent quantitative PCR (Polymerase Chain Reaction) sealing plate membrane which comprises a sealing plate main body, a first gripper is arranged at the left end of the sealing plate main body, a membrane sealing area is arranged on one side of the first gripper, and the membrane sealing area is provided with at least one pre-segmented area. The reaction plate can work according to actual conditions, one pre-partition area is used, in addition, when the film needs to be integrally pasted or detached, the film can still be integrally pasted or detached through the first tongs, waste of materials is effectively avoided, and the utilization rate of the reaction plate is increased.

Description

Technical Field

[0001] The present application relates to the technical field of plate sealing membranes, and in particular to a pre-divided fluorescent quantitative PCR plate sealing membrane. Background Art

[0002] Real-time fluorescence quantitative PCR (qPCR) is a technology that adds fluorescent groups to the PCR reaction system and monitors the amplified products in real time by collecting the fluorescent signals in real time during the reaction. Due to its advantages such as strong specificity, high sensitivity and high accuracy, this technology is widely used in various experimental fields and analytical procedures such as molecular biology, forensic analysis, evolutionary biology and medical diagnosis. During the experiment, the reaction plate containing the qPCR reaction system needs to be sealed with a suitable sealing film to prevent the evaporation of the reaction solution in each well of the reaction plate and reduce cross-contamination between reaction wells, thereby reducing experimental errors and improving experimental accuracy.

[0003] The sealing films currently used are all of a whole-piece design. When you only need to stick or remove the sealing film on a part of the reaction plate, you still need to operate the entire sealing film. This operation method has great risks and shortcomings, especially after the qPCR reaction is completed, the target DNA fragment has been exponentially amplified in the reaction well. At this time, removing the sealing film of the reaction plate as a whole will not only increase the cross-contamination between the reaction wells, but also easily cause contamination of the experimental area; in order to avoid such risks, even if the utilization rate of the reaction plate is very low, the entire reaction plate will still be discarded after the reaction is completed, which will cause great waste and increase the cost of the experiment.

[0004] Therefore, a pre-divided fluorescent quantitative PCR plate sealing membrane is urgently needed. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model aims to provide a plate sealing film that can be pre-segmented and reduces cost waste.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a pre-divided fluorescent quantitative PCR sealing film, comprising a sealing body, a first gripper is provided at the left end of the sealing body, a sealing area is provided on one side of the first gripper, and the sealing area is provided with at least one pre-divided area.

[0007] Preferably, the film sealing area is provided with a first pre-dividing area, a second pre-dividing area is provided on one side of the first pre-dividing area, and a third pre-dividing area is provided on the second pre-dividing area.

[0008] Preferably, a second gripper is disposed at the upper end of the first pre-dividing zone, a third gripper is disposed at the lower end of the second pre-dividing zone, and a fourth gripper is disposed at the upper end of the third pre-dividing zone.

[0009] Preferably, the first pre-dividing area is rectangular, the second pre-dividing area is rectangular, the third pre-dividing area is rectangular, the film sealing area is provided with release paper, and a sealing film is provided on the release paper.

[0010] Compared with the prior art, the beneficial effects of the utility model are:

[0011] The utility model divides the film sealing area into a plurality of pre-divided areas, and can work according to actual conditions, using one of the pre-divided areas. In addition, when the film needs to be pasted or removed as a whole, the first gripper can still be used for integral pasting or removal, thereby effectively avoiding waste of materials and improving the utilization rate of the reaction plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a structural schematic diagram of the utility model;

[0013] In the figure: a first gripper 1; a film sealing area 2; a first pre-dividing area 3; a second pre-dividing area 4; a third pre-dividing area 5; a second gripper 6; a third gripper 7; a fourth gripper 8; and a release paper 9. DETAILED DESCRIPTION

[0014] The following will clearly and completely describe the technical solutions in the embodiments of the utility model in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0015] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0016] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "set / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0017] like Figure 1 As shown, the utility model provides a technical solution: a pre-divided fluorescent quantitative PCR plate sealing film, including a plate sealing body, a first gripper 1 is provided at the left end of the plate sealing body, a sealing film area 2 is provided on one side of the first gripper 1, and the sealing film area 2 is provided with at least one pre-divided area. The sealing film area 2 is provided with a first pre-divided area 3, a second pre-divided area 4 is provided on one side of the first pre-divided area 3, and the second pre-divided area 4 is provided with a third pre-divided area 5; the first gripper 1 is separated from the sealing film area 3 to facilitate taking the plate sealing film.

[0018] A second gripper 6 is provided at the upper end of the first pre-dividing area 3, a third gripper 7 is provided at the lower end of the second pre-dividing area 4, and a fourth gripper 8 is provided at the upper end of the third pre-dividing area 5. The first pre-dividing area 3 is rectangular, the second pre-dividing area 4 is rectangular, and the third pre-dividing area 5 is rectangular. The film sealing area 2 is provided with a release paper 9, and a sealing film is provided on the surface of the release paper 9.

[0019] Working principle of this utility model:

[0020] like Figure 1 As shown, the present application is provided with a second gripper 6, a third gripper 7 and a fourth gripper 8 in the first pre-dividing area 3, the second pre-dividing area 4 and the third pre-dividing area 5, so as to facilitate pasting or removing the film according to the usage. In addition, the sealing film in the pre-dividing area can be torn off from the bottom release paper in a whole piece. If the sealing film area 2 needs to be sealed or demolded as a whole, it can still be pasted or removed as a whole according to the usage practice of the whole-piece sealing film. When one of the pre-dividing areas needs to be sealed or removed, the sealing film of the pre-dividing area can be pasted or applied from the release paper through the corresponding gripper.

[0021] For the convenience of description, the orientations mentioned above are now defined as follows: the up, down, left, right, front, and back directions mentioned above are consistent with the up, down, left, right, front, and back directions of the projection relationship of the image itself.

[0022] The above shows and describes the basic principles and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is defined by the attached claims rather than the above description, and it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0023] The above-described embodiments are only specific implementation methods of the present invention, which are used to illustrate the technical solutions of the present invention, rather than to limit them. The protection scope of the present invention is not limited thereto. Although the present invention is described in detail with reference to the above-described embodiments, ordinary technicians in the field should understand that any technician familiar with the technical field can still modify the technical solutions recorded in the above-described embodiments within the technical scope disclosed by the present invention, or can easily think of changes, or make equivalent replacements for some of the technical features therein; and these modifications, changes or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention, and should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A pre-divided fluorescent quantitative PCR plate sealing membrane, characterized in that: It comprises a sealing plate body, a first gripper (1) is arranged at the left end of the sealing plate body, a film sealing area (2) is arranged on one side of the first gripper (1), and the film sealing area (2) is provided with at least one pre-dividing area; The film sealing area (2) is provided with a first pre-dividing area (3), a second pre-dividing area (4) is provided on one side of the first pre-dividing area (3), and the second pre-dividing area (4) is provided with a third pre-dividing area (5); A second gripper (6) is provided at the upper end of the first pre-dividing area (3), a third gripper (7) is provided at the lower end of the second pre-dividing area (4), and a fourth gripper (8) is provided at the upper end of the third pre-dividing area (5); The first pre-divided area (3) is rectangular, the second pre-divided area (4) is rectangular, and the third pre-divided area (5) is rectangular; The film sealing area (2) is provided with release paper (9).

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