Freezing bead device for fluorescent antibody freeze-dried original solution

Abstract

The utility model relates to the technical field of biology, in particular to a bead freezing device for a fluorescent antibody freeze-dried original solution. The utility model provides a bead freezing device for a fluorescent antibody freeze-dried original solution, which enables liquid nitrogen to circularly flow and prevents the liquid from hanging on a wall in a bead freezing process. The utility model relates to a bead freezing device for a fluorescent antibody freeze-dried original solution. The bead freezing device comprises a base, a console, a bead freezing dropping device, a nitrogen supplementing pipe, a bead freezing tank, a collecting pipe and the like, pressure is generated through the liquid pump, so that liquid nitrogen entering the bead freezing tank can be integrally circulated along the communicating tank, the liquid inlet pipe and the liquid outlet pipe, the liquid nitrogen is prevented from being deposited in the bead freezing tank, and the problem that a fluorescent antibody freeze-drying original solution is hung on a wall in the condensation process can be effectively avoided.

Description

Technical Field

[0001] The utility model relates to the field of biotechnology, in particular to a freezing bead device for freeze-drying a fluorescent antibody original solution. Background Art

[0002] Fluorescent antibody freeze-dried stock solution is a common biological reagent, usually used in experiments such as immunofluorescence staining. This reagent is made by freeze-drying a solution containing specific antibodies into a solid form for long-term storage and stability, and then re-dissolved for use when needed. In the specific use process, the existing fluorescent antibody freeze-dried stock solution frozen bead preparation device generally relies on a drip pump to output the fluorescent antibody freeze-dried stock solution, and quickly condenses the fluorescent antibody freeze-dried stock solution through the low temperature of liquid nitrogen, so that the fluorescent antibody freeze-dried stock solution can be formed in the container. However, under this method, due to operational problems and liquid nitrogen deposition, the fluorescent antibody freeze-dried stock solution will stick to the wall, affecting the final frozen bead forming effect.

[0003] Therefore, in order to solve the above problems, a freezing device for a fluorescent antibody freeze-dried original solution is developed to allow liquid nitrogen to circulate and prevent liquid from adhering to the wall during the freezing process. Utility Model Content

[0004] In order to overcome the shortcomings of the existing devices, which may cause the fluorescent antibody freeze-dried original solution to stick to the wall due to operational problems and liquid nitrogen deposition, thus affecting the final frozen bead forming effect, the utility model provides a freezing bead device for the fluorescent antibody freeze-dried original solution, which allows the liquid nitrogen to circulate and prevents the liquid from sticking to the wall during the freezing process.

[0005] The technical solution of the utility model is: a freezing bead device for freeze-dried original solution of fluorescent antibody, comprising a base, a freezing bead tank connected to the upper right side of the base, a connecting pipe connected to the lower right side of the freezing bead tank, a collecting pipe connected to and communicated with the connecting pipe, a sealing cover clamped on the top of the collecting pipe, a liquid pump connected to the top of the sealing cover, a liquid outlet pipe connected to the upper left side of the liquid pump, the left end of the liquid outlet pipe is located in the freezing bead tank, and a liquid inlet pipe is connected to the bottom of the liquid pump.

[0006] As a preferred technical solution of the utility model, it also includes a console, which is connected to the upper left side of the base, a frozen bead dropper is connected to the upper right side of the console, a nitrogen supplement pipe is connected to the lower right side of the console, and the nitrogen supplement pipe is connected and communicated with the upper left side of the frozen bead tank.

[0007] As a preferred technical solution of the utility model, it also includes a fixing column, the lower side of the right part of the sealing cover is connected to the fixing column, and the bottom end of the fixing column is connected to a filter basket.

[0008] As a preferred technical solution of the present utility model, the connecting pipe is connected and communicated with the frozen bead tank.

[0009] As a preferred technical solution of the present utility model, the connecting pipe is an upwardly inclined structure.

[0010] As a preferred technical solution of the utility model, a control valve is installed at the left end of the nitrogen replenishing pipe near the control console.

[0011] By adopting the above technical solution, the beneficial effects of the utility model are:

[0012] The utility model generates pressure through a liquid pump, so that the liquid nitrogen entering the freezing bead tank can be circulated as a whole along the connecting tank, the liquid inlet pipe and the liquid outlet pipe, thereby preventing the liquid nitrogen from being deposited inside the freezing bead tank and effectively avoiding the problem of wall adhesion during the condensation process of the fluorescent antibody freeze-dried original solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a three-dimensional structural schematic diagram of the utility model.

[0014] Figure 2 It is a partial three-dimensional structural schematic diagram of the utility model.

[0015] Figure numbers: 1_base, 2_control console, 3_frozen bead dropper, 4_nitrogen replenishing tube, 5_frozen bead tank, 6_collecting tube, 7_connecting tube, 8_sealing cover, 9_liquid pump, 10_liquid outlet pipe, 11_liquid inlet pipe, 12_fixed column, 13_filter basket. DETAILED DESCRIPTION

[0016] The utility model is described in detail below in conjunction with the accompanying drawings.

[0017] A freeze bead device for freeze-drying a fluorescent antibody stock solution, such as Figure 1-2 As shown, it includes a base 1, and a bead tank 5 for forming freeze-dried original solution into beads is connected to the upper right side of the base 1, and a connecting pipe 7 for guiding the movement of frozen beads is connected to the lower right side of the bead tank 5, and the connecting pipe 7 is connected and communicated with the bead tank 5, and the connecting pipe 7 is an upward inclined structure, and a collecting pipe 6 is connected to the upper right side of the base 1, and the collecting pipe 6 is connected and communicated with the connecting pipe 7, and a sealing cover 8 is clamped on the top of the collecting pipe 6, and a liquid pump 9 is connected to the top of the sealing cover 8, and a liquid outlet pipe 10 is connected to the upper left side of the liquid pump 9, and the left end of the liquid outlet pipe 10 is located in the bead tank 5, and a liquid inlet pipe 11 is connected to the bottom of the liquid pump 9.

[0018] like Figure 1As shown, a control console 2 is also included. The control console 2 is connected to the upper left side of the base 1, a frozen bead dropper 3 is connected to the upper right side of the control console 2, a nitrogen supply pipe 4 is connected to the lower right side of the control console 2, the nitrogen supply pipe 4 is connected to and communicated with the upper left side of the frozen bead tank 5, and a control valve is installed at the left end of the nitrogen supply pipe 4 near the control console 2.

[0019] like Figure 2 As shown, a fixing column 12 is also included. The fixing column 12 is connected to the lower right side of the sealing cover 8. The bottom end of the fixing column 12 is connected to a filter basket 13 for drying and collecting the formed frozen beads.

[0020] It should be noted that the present device can be used to assist in the frozen bead preparation operation. First, the prepared fluorescent antibody freeze-dried original solution is added to the frozen bead dropper 3. Then, the control console 2 is operated to open the control valve on the nitrogen replenishment tube 4. Liquid nitrogen enters the frozen bead tank 5 through the nitrogen replenishment tube 4. At the same time, the liquid pump 9 is started. As the liquid pump 9 starts to work, the liquid nitrogen in the frozen bead tank 5 will enter the collecting tube 6 through the connecting tube 7, and enter the liquid inlet pipe 11 under the action of the liquid pump 9. Then, the liquid nitrogen is discharged through the liquid outlet pipe 10 by the liquid pump 9, so that the liquid nitrogen can form a flow circulation in the frozen bead tank 5 to prevent the liquid nitrogen from being deposited at the bottom of the frozen bead tank 5. Then, the frozen bead dropper 3 is started, so that the configured fluorescent antibody freeze-dried original solution can begin to drip into the frozen bead tank 5. As the fluorescent antibody freeze-dried original solution drips into the frozen bead tank 5, under the low temperature of liquid nitrogen, the fluorescent antibody freeze-dried original solution can be quickly condensed to form frozen beads. The formed frozen beads are small in size and light in weight. They will enter the connecting pipe 7 together with the flowing liquid nitrogen. When the frozen beads leave the connecting pipe 7, they will directly fall into the filter basket 13 to complete automatic collection. After the frozen beads are prepared, the frozen bead dropper 3 and the liquid pump 9 are closed, and the control console 2 is operated to stop the liquid nitrogen output. Finally, the sealing cover 8 is separated from the collection tube 6, and the frozen beads in the filter basket 13 can be taken out.

[0021] Those skilled in the art should understand that the above embodiments do not limit the present invention in any form, and any technical solutions obtained by equivalent replacement or equivalent transformation shall fall within the protection scope of the present invention.

Claims

1. A freezing bead device for freeze-dried fluorescent antibody stock solution, comprising a base (1), a freezing bead tank (5) connected to the upper right side of the base (1), and a connecting pipe (7) connected to the lower right side of the freezing bead tank (5), characterized in that: A collecting tube (6) is connected to the upper right side of the base (1), and the collecting tube (6) is connected and communicated with the connecting tube (7). A sealing cover (8) is clamped on the top of the collecting tube (6), and a liquid pump (9) is connected to the top of the sealing cover (8). A liquid outlet pipe (10) is connected to the left side of the upper part of the liquid pump (9), and the left end of the liquid outlet pipe (10) is located in the frozen bead tank (5). The bottom of the liquid pump (9) is connected to a liquid inlet pipe (11).

2. A freezing bead device for freeze-drying a fluorescent antibody stock solution as claimed in claim 1, characterized in that: The system further comprises a control console (2), wherein the upper left side of the base (1) is connected to the control console (2), the upper right side of the control console (2) is connected to a frozen bead dropper (3), the lower right side of the control console (2) is connected to a nitrogen supply pipe (4), and the nitrogen supply pipe (4) is connected to and communicated with the upper left side of the frozen bead tank (5).

3. A freezing bead device for freeze-dried fluorescent antibody stock solution as claimed in claim 2, characterized in that: It also comprises a fixing column (12), the lower right side of the sealing cover (8) is connected to the fixing column (12), and the bottom end of the fixing column (12) is connected to a filter basket (13).

4. The freezing bead device for freeze-drying fluorescent antibody stock solution according to claim 1, characterized in that: The connecting pipe (7) is connected to and communicates with the bead tank (5).

5. The freezing bead device for freeze-drying fluorescent antibody stock solution according to claim 1, characterized in that: The connecting pipe (7) is an upwardly inclined structure.

6. A freezing bead device for freeze-drying a fluorescent antibody stock solution as claimed in claim 2, characterized in that: A control valve is installed at the left end of the nitrogen replenishing pipe (4) near the control console (2).

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