Culture medium for tissue culture

Abstract

The utility model provides a culture medium for tissue culture, which belongs to the technical field of plant tissue culture and comprises a culture box and a cover plate placed on the upper end face of the culture box, a fixed partition plate is fixedly mounted in the middle of the bottom face of an inner cavity of the culture box, and a first annular groove and a second annular groove are formed in the middle of the side wall of the fixed partition plate. A first partition plate is slidably mounted in an inner cavity of the first annular groove, a second partition plate is slidably mounted in an inner cavity of the second annular groove, and a locking mechanism is arranged in the middle of an inner cavity of the culture box. Through the arrangement of the locking mechanism, the first partition plate and the second partition plate can be adjusted in a sliding manner, so that the internal structure of the culture box is changed, the culture box is suitable for culture of different experimental tissues, the number of culture media consumed when contrast experiments are carried out on the experimental tissues is reduced, and the culture box is convenient and practical; the sealing effect between the cover plate and the culture box can be ensured, and the accuracy during experimental tissue culture is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of plant tissue culture, in particular to a culture medium for tissue culture. Background Art

[0002] Dendrobium officinale belongs to the genus Dendrobium of the Orchidaceae family. Most of them grow on semi-shaded and moist rocks in mountains above 1,000 meters above sea level. They prefer warm and humid environments and are extremely cold-resistant. They are suitable for growing in cool, humid, and well-ventilated environments. Because the seeds of Dendrobium officinale are very small and have no embryos, the germination rate under natural conditions is low (less than 5%) and it can only germinate under the condition of the presence of orchid fungi. The reproduction rate is extremely low. In addition, the pharmacological value of Dendrobium officinale is high, and people dig a large number of them, and Dendrobium officinale cannot be supplied to people for use.

[0003] In order to protect this endangered precious Chinese medicine, researchers at home and abroad have carried out research on tissue culture rapid propagation, seed germination and mycorrhizal reproduction of Dendrobium officinale. However, the existing culture medium used to study Dendrobium officinale is limited to optimizing and improving some links of rapid propagation when used, and it is impossible to improve the internal structure of the culture medium. Therefore, in the experiment, more culture medium needs to be used for comparative experiments, which is complicated and cumbersome, and it is difficult to meet the use requirements in the laboratory research process. Therefore, how to invent a tissue culture medium to improve these problems has become a problem that needs to be solved urgently by those skilled in the art. Utility Model Content

[0004] In order to make up for the above shortcomings, the utility model provides a culture medium for tissue culture, aiming to improve the problem that the existing culture medium is inconvenient to improve the internal structure during use.

[0005] The utility model is achieved in this way:

[0006] The utility model provides a culture medium for tissue culture, comprising a culture box and a cover plate placed on the upper end surface of the culture box, a handle is fixedly installed in the middle of the upper end surface of the cover plate, a fixed partition is fixedly installed in the middle of the inner cavity bottom surface of the culture box, a first annular groove and a second annular groove are opened in the middle of the side wall of the fixed partition, the first annular groove is slidably installed with the first partition plate in the inner cavity, the second annular groove is slidably installed with the second partition plate in the inner cavity, a locking mechanism is arranged in the middle of the inner cavity of the culture box, and a sealing mechanism is arranged at the lower part of the side wall of the cover plate.

[0007] Preferably, the locking mechanism comprises a screw rod fixedly mounted in the middle of the bottom surface of the inner cavity of the culture box, and a locking nut is threadedly mounted on the upper part of the side wall of the screw rod.

[0008] By adopting the above technical solution, the space of the internal compartment of the culture box can be adjusted.

[0009] Preferably, the screw rod is connected through the first partition plate and the second partition plate, the locking nut is in contact with the first partition plate, and the cover plate is made of transparent glass.

[0010] By adopting the above technical solution, the inside of the culture box can be viewed through the cover.

[0011] Preferably, a temperature sensor and a humidity sensor are fixedly mounted on the outer side of the bottom surface of the inner cavity of the culture box, a control display is fixedly mounted on the upper end surface of the cover plate, a sterilization lamp is fixedly mounted on the middle part of the lower end surface of the cover plate, and the control display is electrically connected to the temperature sensor, humidity sensor and sterilization lamp.

[0012] By adopting the above technical solution, the growth environment of the plant tissue in the culture box can be monitored in real time.

[0013] Preferably, the sealing mechanism includes a mounting seat fixedly mounted around the lower end surface of the cover plate, a locking rod is slidably mounted on the lower part of the inner cavity of the mounting seat, an end plate is fixedly mounted on the front end surface of the locking rod, and a return spring is wound around the side wall of the locking rod.

[0014] By adopting the above technical solution, the cover plate can be firmly covered on the culture box.

[0015] Preferably, both ends of the reset spring are fixedly mounted on the end plate and the side wall of the mounting seat respectively, a locking groove is opened on the upper side of the side wall of the culture box, the end of the locking rod is against the bottom surface of the inner cavity of the locking groove, and the end of the locking rod is set to be rounded.

[0016] The beneficial effects of the utility model are:

[0017] The user first loosens the locking nut from the screw rod, and then rotates the first partition and the second partition to cooperate with the fixed partition to change the layout of the entire culture box. At this time, the soil for plant tissue culture is laid on the bottom of the culture box, and then the plant tissues for experimental comparison are placed in different compartments of the culture box. Therefore, the culture medium can be suitable for different varieties of plant tissues by changing the space of the internal compartments of the culture box, thereby improving the practicality of the entire culture medium; by setting the sterilization lamp on the cover, all the compartments in the culture box can be sterilized to ensure a sterile environment for plant tissue culture, and at the same time, by setting the sealing mechanism, the locking rod can be engaged in the locking groove, thereby improving the sealing effect of the internal space of the culture box and ensuring the accuracy of experimental tissue culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solution of the implementation mode of the utility model, the drawings required for use in the implementation mode will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 It is a schematic diagram of the overall structure of a tissue culture medium provided by an embodiment of the utility model;

[0020] Figure 2 It is a schematic diagram of the internal structure of a culture box of a tissue culture medium provided by an embodiment of the utility model;

[0021] Figure 3 It is a partial structural schematic diagram of a cover plate of a tissue culture medium provided by an embodiment of the utility model;

[0022] Figure 4 It is a schematic cross-sectional view of a culture box of a tissue culture medium provided by an embodiment of the utility model;

[0023] Figure 5 A tissue culture medium provided by the utility model Figure 4 Schematic diagram of the enlarged structure of area A in the middle.

[0024] In the figure: 1. culture box; 11. temperature sensor; 12. humidity sensor; 13. control display; 14. sterilization lamp; 2. cover plate; 3. handle; 4. fixed partition; 41. first ring groove; 42. second ring groove; 5. first partition; 6. second partition; 7. locking mechanism; 71. screw; 72. locking nut; 8. sealing mechanism; 81. mounting seat; 82. locking rod; 83. end plate; 84. reset spring; 85. locking groove. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the implementation of the utility model clearer, the technical solution in the implementation of the utility model will be clearly and completely described below in conjunction with the drawings in the implementation of the utility model. Obviously, the described implementation is a part of the implementation of the utility model, not all of the implementations. Based on the implementation of the utility model, all other implementations obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Example

[0027] Reference Figure 1-5A culture medium for tissue culture comprises a culture box 1 and a cover plate 2 placed on the upper end surface of the culture box 1, a handle 3 is fixedly installed in the middle of the upper end surface of the cover plate 2, a fixed partition plate 4 is fixedly installed in the middle of the bottom surface of the inner cavity of the culture box 1, a first annular groove 41 and a second annular groove 42 are opened in the middle of the side wall of the fixed partition plate 4, a first partition plate 5 is slidably installed in the inner cavity of the first annular groove 41, and a second partition plate 6 is slidably installed in the inner cavity of the second annular groove 42, a locking mechanism 7 is arranged in the middle of the inner cavity of the culture box 1, and a sealing mechanism 8 is arranged at the lower part of the side wall of the cover plate 2.

[0028] Furthermore; the locking mechanism 7 includes a screw 71 fixedly installed in the middle of the bottom surface of the inner cavity of the culture box 1, and a locking nut 72 is threadedly installed on the upper part of the side wall of the screw 71. The screw 71 is connected through the first partition 5 and the second partition 6, and the locking nut 72 is in contact with the first partition 5. The cover 2 is made of transparent glass. A temperature sensor 11 and a humidity sensor 12 are fixedly installed on the outer side of the bottom surface of the inner cavity of the culture box 1, a control display 13 is fixedly installed on the upper end surface of the cover 2, and a sterilization lamp 14 is fixedly installed in the middle of the lower end surface of the cover 2. The control display 13 is electrically connected to the temperature sensor 11, the humidity sensor 12 and the sterilization lamp 14.

[0029] It should be noted that: the user first loosens the locking nut 72 from the screw 71, and then rotates the first partition 5 and the second partition 6 to cooperate with the fixed partition 4 to change the layout of the entire culture box 1. At this time, the soil for plant tissue culture is laid on the bottom surface of the culture box 1, and then the plant tissues for experimental comparison are placed in different compartments of the culture box 1. Through the above, the culture medium can be suitable for different varieties of plant tissues by changing the space of the internal compartments of the culture box 1, thereby improving the practicality of the entire culture medium.

[0030] Furthermore, the sealing mechanism 8 includes a mounting seat 81 fixedly installed around the lower end surface of the cover plate 2, a locking rod 82 is slidably installed on the lower part of the inner cavity of the mounting seat 81, an end plate 83 is fixedly installed on the front end surface of the locking rod 82, a return spring 84 is wound and installed on the side wall of the locking rod 82, and the two ends of the return spring 84 are respectively fixedly installed on the end plate 83 and the side wall of the mounting seat 81, a locking groove 85 is opened on the upper side of the side wall of the culture box 1, the end of the locking rod 82 is against the bottom surface of the inner cavity of the locking groove 85, and the end of the locking rod 82 is set to be rounded.

[0031] It should be noted that: after the locking nut 72 is tightened from the screw 71 to fix the first partition 5 and the second partition 6, the user places the cover 2 on the culture box 1. By setting the sterilization lamp 14 on the cover 2, all the compartments in the culture box 1 can be sterilized, ensuring a sterile environment for plant tissue culture. When the cover 2 is closed on the culture box 1, the locking rod 82 contacts the outer wall of the culture box 1, and the locking rod 82 will first squeeze the reset spring 84 to move the end plate 83 outward. When the locking rod 82 moves to the position of the locking groove 85 on the culture box 1, the reset spring 84 is retracted. At this time, the locking rod 82 will be engaged in the locking groove 85 to fix the cover 2, thereby ensuring the sealing of the internal space of the culture box 1.

[0032] How this tissue culture medium works:

[0033] The user first loosens the locking nut 72 from the screw rod 71, and then rotates the first partition 5 and the second partition 6 to cooperate with the fixed partition 4 to change the pattern inside the entire culture box 1. At this time, the soil for plant tissue culture is laid on the bottom surface of the culture box 1, and then the plant tissues for experimental comparison are placed in different compartments of the culture box 1. As described above, the culture medium can be used for different varieties of plant tissues by changing the space of the internal compartments of the culture box 1, thereby improving the practicality of the entire culture medium;

[0034] After the locking nut 72 is tightened from the screw 71 to fix the first partition 5 and the second partition 6, the user places the cover 2 on the culture box 1. By setting the sterilization lamp 14 on the cover 2, all the compartments in the culture box 1 can be sterilized, ensuring a sterile environment for plant tissue culture. When the cover 2 is closed on the culture box 1, the locking rod 82 contacts the outer wall of the culture box 1. The locking rod 82 will first squeeze the reset spring 84 to move the end plate 83 outward. When the locking rod 82 moves to the position of the locking groove 85 on the culture box 1, the reset spring 84 is retracted. At this time, the locking rod 82 will be engaged in the locking groove 85 to fix the cover 2, thereby ensuring the sealing of the internal space of the culture box 1.

[0035] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "space", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicating orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0036] It should be noted that the specific model and specifications of the motor need to be selected and determined based on the actual specifications of the device, and the specific selection calculation method adopts the existing technology in this field, so it will not be described in detail.

[0037] The above description is only the preferred implementation of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A culture medium for tissue culture, comprising a culture box (1), and a cover plate (2) placed on the upper end surface of the culture box (1), wherein a handle (3) is fixedly mounted in the middle of the upper end surface of the cover plate (2), characterized in that: A fixed partition (4) is fixedly installed in the middle of the bottom surface of the inner cavity of the culture box (1); a first annular groove (41) and a second annular groove (42) are opened in the middle of the side wall of the fixed partition (4); a first partition (5) is slidably installed in the inner cavity of the first annular groove (41); a second partition (6) is slidably installed in the inner cavity of the second annular groove (42); a locking mechanism (7) is arranged in the middle of the inner cavity of the culture box (1); and a sealing mechanism (8) is arranged at the lower part of the side wall of the cover plate (2).

2. A tissue culture medium according to claim 1, characterized in that: The locking mechanism (7) comprises a screw rod (71) fixedly mounted in the middle of the bottom surface of the inner cavity of the culture box (1), and a locking nut (72) is threadedly mounted on the upper part of the side wall of the screw rod (71).

3. A tissue culture medium according to claim 2, characterized in that: The screw rod (71) is connected through the first partition plate (5) and the second partition plate (6); the locking nut (72) is in contact with the first partition plate (5); and the cover plate (2) is made of transparent glass.

4. A tissue culture medium according to claim 1, characterized in that: A temperature sensor (11) and a humidity sensor (12) are fixedly mounted on the outer side of the bottom surface of the inner cavity of the culture box (1); a control display (13) is fixedly mounted on the upper end surface of the cover plate (2); a sterilization lamp (14) is fixedly mounted on the middle part of the lower end surface of the cover plate (2); and the control display (13) is electrically connected to the temperature sensor (11), the humidity sensor (12) and the sterilization lamp (14).

5. A tissue culture medium according to claim 1, characterized in that: The sealing mechanism (8) comprises a mounting seat (81) fixedly mounted around the lower end surface of the cover plate (2); a locking rod (82) is slidably mounted in the lower part of the inner cavity of the mounting seat (81); an end plate (83) is fixedly mounted on the front end surface of the locking rod (82); and a return spring (84) is wound around the side wall of the locking rod (82).

6. A tissue culture medium according to claim 5, characterized in that: The two ends of the return spring (84) are respectively fixedly mounted on the end plate (83) and the side wall of the mounting seat (81); a locking groove (85) is provided on the upper side of the side wall of the culture box (1); the end of the locking rod (82) abuts against the bottom surface of the inner cavity of the locking groove (85); and the end of the locking rod (82) is arranged to be rounded.

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