Cultivation device for halophilic bacteria in high-salt environment

Abstract

The utility model provides a cultivation device for halophilic bacteria in a high-salinity environment, and relates to the technical field of high-salinity wastewater treatment. An inner container is arranged in the cultivation box, first motor seats are installed on the inner walls of the left side and the right side of the inner container through bolts, first motors are installed at the lower ends of the first motor seats through bolts, and bearing seats are installed on the inner walls of the left side and the right side of the inner container through bolts; a threaded rod is mounted between the bearing seat and the first motor seat through a bearing; a culture solution is quantitatively added into the culture dish through the filling head, the top cover is in a closed state in the nutrient solution adding process, the opportunity that halophilic bacteria make contact with the external environment is reduced, the pollution risk of the halophilic bacteria is reduced, the first transmission belt is operated by operating the second motor, the front-back movement of the filling head can be achieved, and the filling efficiency is improved. A third motor is operated to enable a second transmission belt to operate, the filling head can move left and right, and quantitative adding of the nutrient solution to the culture dish is facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of high-salt wastewater treatment, in particular to a device for cultivating halophilic bacteria in a high-salt environment. Background Art

[0002] Halophiles are a type of bacteria that can live in high-salinity environments. When treating some saline organic wastewater, the presence of salt usually has a significant inhibitory effect on conventional biological treatment. However, due to the unique structural composition, physiological functions and metabolites of halophiles, halophiles are special microorganisms that can degrade organic pollutants in high-salinity environments. When using halophilic bacteria to treat sewage, it is necessary to use a halophilic bacteria cultivation device to cultivate the halophilic bacteria.

[0003] Existing halophilic bacteria cultivation equipment cannot provide a consistent high-salt environment on an experimental and industrial scale, and the salt concentration is not precisely controlled, resulting in limited growth of halophilic bacteria. Utility Model Content

[0004] The utility model provides a halophilic bacteria cultivation device in a high-salt environment, so as to solve the problem that the salt concentration of the existing halophilic bacteria cultivation device is not accurately controlled, resulting in the growth restriction of the halophilic bacteria.

[0005] The utility model provides a halophilic bacteria cultivation device in a high-salt environment, comprising: a cultivation box; pull-up grooves are arranged on the left and right sides of the cultivation box, and an inner liner is arranged inside the cultivation box, a No. 1 motor seat is installed on the inner walls on the left and right sides of the inner liner through bolts, a No. 1 motor is installed on the lower end of the No. 1 motor seat through bolts, and bearing seats are installed on the inner walls on the left and right sides of the inner liner through bolts, a threaded rod is installed between the bearing seat and the No. 1 motor seat through a bearing, the lower end of the threaded rod is connected to the output shaft of the No. 1 motor, and a vertical rod is installed between the bearing seat and the No. 1 motor seat through bolts, a threaded block is matched with a thread on the threaded rod, two groups of bearings are installed on the threaded block, the two groups of bearings are respectively in contact with the front and rear ends of the vertical rod, a connecting frame is installed on the threaded block through bolts, a support plate is fixed between the two groups of connecting frames, and a culture dish is arranged on the support plate.

[0006] Preferably, a top cover is hingedly connected to the upper end of the incubator, a fixing frame is fixed to the lower end of the top cover, a No. 1 slide bar is fixed to both left and right sides of the fixing frame, and a rotating column is installed on the fixing frame through a bearing.

[0007] Preferably, a No. 1 sliding seat and a No. 2 sliding seat are respectively slid on the two groups of No. 1 sliding rods, and a No. 2 motor seat is installed on the fixed frame by bolts, and a No. 2 motor is installed on the No. 2 motor seat by bolts, and the output shaft of the No. 2 motor is connected to the rotating column by a belt.

[0008] Preferably, two sets of connecting seats are installed at the rear end of the fixed frame by bolts, and a No. 1 transmission belt is connected between the two sets of connecting seats and the rotating column. The two sets of No. 1 transmission belts are fixedly connected to the No. 1 sliding seat and the No. 2 sliding seat respectively by bolts.

[0009] Preferably, two groups of No. 2 sliding rods are fixed between the No. 1 sliding seat and the No. 2 sliding seat, a moving seat is slid on the two groups of No. 2 sliding rods, a filling head is installed on the moving seat through bolts, the filling head is connected to the external culture medium supply equipment through a connecting pipe, and a connecting wheel is installed on the lower end of the No. 1 sliding seat through a bearing, a No. 3 motor is installed on the lower end of the No. 2 sliding seat through bolts, a pulley is installed on the output shaft of the No. 3 motor, a No. 2 transmission belt is connected between the pulley on the output shaft of the No. 3 motor and the connecting wheel, and the lower end of the moving seat is fixedly connected to the No. 2 transmission belt.

[0010] Preferably, a side plate is fixed on the movable seat, two groups of No. 3 slide bars are fixed on the side plate, a lifting plate is slid on the two groups of No. 3 slide bars, and a hydraulic rod is installed on the side plate by bolts, the piston rod of the hydraulic rod is connected to the lifting plate, a mounting seat is installed on the lifting plate by bolts, and a detection head is fixed on the mounting seat.

[0011] The beneficial effects of the halophilic bacteria cultivation device in a high-salt environment of the utility model are as follows:

[0012] (1) A filling head is provided at the lower end of the top cover of the cultivation device of the utility model, and the culture solution is quantitatively added to the culture dish through the filling head. In the process of adding the nutrient solution, the top cover is in a closed state, which reduces the chance of the halophilic bacteria contacting the external environment and reduces the risk of contamination of the halophilic bacteria. In addition, by operating the No. 2 motor to operate the No. 1 transmission belt, the filling head can be moved forward and backward, and by operating the No. 3 motor to operate the No. 2 transmission belt, the filling head can be moved left and right, which is convenient for adding nutrient solution to the culture dish.

[0013] (2) A side plate is fixed on the movable seat in the cultivation device of the utility model, and a detection head is arranged on the side plate. The detection head is lowered by extending the hydraulic rod and inserted into the culture liquid in the culture dish. The salt concentration of the culture liquid is monitored by the detection head, and the change of the salt concentration can be monitored in real time. The support plate is raised by running the No. 1 motor, which is convenient for taking and placing the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the cultivation device of the utility model of halophilic bacteria in a high-salt environment;

[0015] Figure 2 It is a structural schematic diagram of a support plate in a cultivation device for halophilic bacteria in a high-salt environment of the utility model;

[0016] Figure 3 yes Figure 2 A schematic diagram of the enlarged structure of part A;

[0017] Figure 4 It is a schematic diagram of the structure of the top cover of the cultivation device of halophilic bacteria in a high-salt environment of the utility model;

[0018] Figure 5 yes Figure 4 A schematic diagram of the enlarged structure of part B;

[0019] Figure 6 yes Figure 4 A schematic diagram of the enlarged structure of the middle C part;

[0020] Figure 7 It is a structural schematic diagram of a No. 1 sliding seat and a No. 2 sliding seat in a cultivation device for halophilic bacteria in a high-salt environment of the utility model;

[0021] Figure 8 It is a structural schematic diagram of a movable seat in a cultivation device of halophilic bacteria in a high-salt environment of the utility model;

[0022] List of reference numerals:

[0023] 1. Cultivation box; 11. Lifting trough; 12. Inner liner; 13. No. 1 motor seat; 131. Bearing seat; 132. No. 1 motor; 133. Vertical rod; 134. Threaded rod; 135. Threaded block; 136. Connecting frame; 14. Support plate; 141. Culture dish;

[0024] 2. Top cover; 21. Fixed frame; 211. No. 2 motor seat; 212. No. 2 motor; 22. No. 1 slide bar; 23. Rotating column; 231. Connecting seat; 232. No. 1 transmission belt; 24. No. 1 sliding seat; 241. No. 2 slide bar; 242. Connecting wheel; 25. No. 2 sliding seat; 251. No. 3 motor; 252. No. 2 transmission belt; 26. Moving seat; 261. Filling head; 27. Side plate; 271. No. 3 slide bar; 272. Lifting plate; 2721. Mounting seat; 2722. Detection head; 273. Hydraulic rod. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution of the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings of the embodiment of the utility model.

[0026] Example 1: Please refer to Figures 1 to 8 :

[0027] The utility model proposes a halophilic bacteria cultivation device in a high-salt environment, comprising: a cultivation box 1; a pull-up groove 11 is arranged on the left and right sides of the cultivation box 1, and an inner liner 12 is arranged inside the cultivation box 1, a No. 1 motor seat 13 is installed on the inner walls on the left and right sides of the inner liner 12 by bolts, a No. 1 motor 132 is installed on the lower end of the No. 1 motor seat 13 by bolts, and a bearing seat 131 is installed on the inner walls on the left and right sides of the inner liner 12 by bolts, and a bearing is installed between the bearing seat 131 and the No. 1 motor seat 13. There is a threaded rod 134, the lower end of the threaded rod 134 is connected to the output shaft of the No. 1 motor 132, and a vertical rod 133 is installed between the bearing seat 131 and the No. 1 motor seat 13 by bolts, and a threaded block 135 is threadedly matched on the threaded rod 134, and two groups of bearings are installed on the threaded block 135, and the two groups of bearings are respectively in contact with the front and rear ends of the vertical rod 133, and a connecting frame 136 is installed on the threaded block 135 by bolts, and a support plate 14 is fixed between the two groups of connecting frames 136, and six groups of culture dishes 141 are arranged on the support plate 14.

[0028] The upper end of the incubator 1 is hinged with a top cover 2, and a fixed frame 21 is fixed at the lower end of the top cover 2. A No. 1 slide bar 22 is fixed on both sides of the fixed frame 21, and a rotating column 23 is installed on the fixed frame 21 through a bearing; a No. 1 sliding seat 24 and a No. 2 sliding seat 25 are respectively slid on the two groups of No. 1 slide bars 22, and a No. 2 motor seat 211 is installed on the fixed frame 21 through bolts, and a No. 2 motor 212 is installed on the No. 2 motor seat 211 through bolts, and the output shaft of the No. 2 motor 212 is connected to the rotating column through a belt. The column 23 is connected; two groups of connecting seats 231 are installed at the rear end of the fixed frame 21 by bolts, and a No. 1 transmission belt 232 is connected between the two groups of connecting seats 231 and the rotating column 23, and the two groups of No. 1 transmission belts 232 are fixedly connected to the No. 1 sliding seat 24 and the No. 2 sliding seat 25 by bolts respectively; its function is: running the No. 2 motor 212 to rotate the rotating column 23, and the rotating column 23 drives the No. 1 sliding seat 24 and the No. 2 sliding seat 25 to slide back and forth through the No. 1 transmission belt 232, so as to realize the adjustment of the front and rear position of the filling head 261.

[0029] Embodiment 2: On the basis of embodiment 1, two groups of No. 2 slide bars 241 are fixed between the No. 1 slide seat 24 and the No. 2 slide seat 25, and a movable seat 26 is slid on the two groups of No. 2 slide bars 241, and a filling head 261 is installed on the movable seat 26 by bolts, and the filling head 261 is connected to the external culture medium supply equipment through a connecting pipe, and a connecting wheel 242 is installed on the lower end of the No. 1 slide seat 24 through a bearing, and a No. 3 motor 251 is installed on the lower end of the No. 2 slide seat 25 by bolts, and a pulley is installed on the output shaft of the No. 3 motor 251, and a No. 2 transmission belt 252 is connected between the pulley on the output shaft of the No. 3 motor 251 and the connecting wheel 242, and the lower end of the movable seat 26 is fixedly connected to the No. 2 transmission belt 252; its function is: running the No. 3 motor 251 makes the No. 2 transmission belt 252 operate, and the No. 2 transmission belt 252 can drive the movable seat 26 to slide left and right, so as to adjust the left and right position of the filling head 261.

[0030] Embodiment 3: On the basis of Embodiment 1 and Embodiment 2, a side plate 27 is fixed on the movable seat 26, two groups of No. 3 slide bars 271 are fixed on the side plate 27, a lifting plate 272 slides on the two groups of No. 3 slide bars 271, and a hydraulic rod 273 is installed on the side plate 27 by bolts, the piston rod of the hydraulic rod 273 is connected to the lifting plate 272, a mounting seat 2721 is installed on the lifting plate 272 by bolts, and a detection head 2722 is fixed on the mounting seat 2721; its function is: by extending the hydraulic rod 273, the detection head 2722 is lowered and inserted into the culture solution in the culture dish 141, the salt concentration of the culture solution is monitored by the detection head 2722, and the salt concentration change can be monitored in real time.

[0031] The working process and use method of the embodiment of the halophilic bacteria cultivation device in a high-salt environment of the utility model are as follows: quantitatively add culture solution to the culture dish 141 through the filling head 261, and the top cover 2 is in a closed state during the process of adding nutrient solution, thereby reducing the chance of contact between the halophilic bacteria and the external environment and reducing the risk of contamination of the halophilic bacteria. The second motor 212 is operated to rotate the rotating column 23, and the rotating column 23 drives the first sliding seat 24 and the second sliding seat 25 to slide forward and backward through the first transmission belt 232, so as to realize the filling head 26 1 front and rear position adjustment, operate the No. 3 motor 251 to operate the No. 2 transmission belt 252, and the No. 2 transmission belt 252 can drive the movable seat 26 to slide left and right, so as to adjust the left and right position of the filling head 261, extend the hydraulic rod 273 to make the detection head 2722 descend and insert it into the culture liquid in the culture dish 141, and monitor the salt concentration of the culture liquid through the detection head 2722, so as to monitor the change of salt concentration in real time, operate the No. 1 motor 132 to make the support plate 14 rise, so as to facilitate the taking and placing of the culture dish 141.

Claims

1. A device for cultivating halophilic bacteria in a high-salt environment, characterized in that: include: A cultivation box (1); the cultivation box (1) is provided with lifting grooves (11) on the left and right sides, and an inner liner (12) is provided inside the cultivation box (1); a No. 1 motor seat (13) is installed on the inner walls on the left and right sides of the inner liner (12) by bolts, a No. 1 motor (132) is installed on the lower end of the No. 1 motor seat (13) by bolts, and a bearing seat (131) is installed on the inner walls on the left and right sides of the inner liner (12) by bolts, and a threaded rod (134) is installed between the bearing seat (131) and the No. 1 motor seat (13) by a bearing, and the threaded rod (134) is installed between the bearing seat (131) and the No. 1 motor seat (13). The lower end of the bearing seat (131) and the motor seat (132) are connected to the output shaft of the first motor (132), and a vertical rod (133) is installed between the bearing seat (131) and the first motor seat (13) by bolts, and a threaded block (135) is threadedly matched on the threaded rod (134), and two groups of bearings are installed on the threaded block (135), and the two groups of bearings are respectively in contact with the front and rear ends of the vertical rod (133), and a connecting frame (136) is installed on the threaded block (135) by bolts, and a support plate (14) is fixed between the two groups of connecting frames (136), and a culture dish (141) is arranged on the support plate (14).

2. The device for cultivating halophilic bacteria in a high-salt environment according to claim 1, characterized in that: The upper end of the incubator (1) is hingedly connected to a top cover (2), the lower end of the top cover (2) is fixed with a fixed frame (21), the left and right sides of the fixed frame (21) are both fixed with a first slide bar (22), and a rotating column (23) is mounted on the fixed frame (21) via a bearing.

3. The device for cultivating halophilic bacteria in a high-salt environment according to claim 2, characterized in that: A No. 1 sliding seat (24) and a No. 2 sliding seat (25) are respectively slidably mounted on the two groups of No. 1 sliding bars (22), and a No. 2 motor seat (211) is mounted on the fixed frame (21) by bolts, a No. 2 motor (212) is mounted on the No. 2 motor seat (211) by bolts, and an output shaft of the No. 2 motor (212) is connected to the rotating column (23) by a belt.

4. The device for cultivating halophilic bacteria in a high-salt environment according to claim 2, characterized in that: Two groups of connecting seats (231) are installed at the rear end of the fixed frame (21) by means of bolts, and a No. 1 transmission belt (232) is connected between the two groups of connecting seats (231) and the rotating column (23), and the two groups of No. 1 transmission belts (232) are fixedly connected to the No. 1 sliding seat (24) and the No. 2 sliding seat (25) by means of bolts, respectively.

5. The device for cultivating halophilic bacteria in a high-salt environment according to claim 4, characterized in that: Two groups of No. 2 slide bars (241) are fixed between the No. 1 slide seat (24) and the No. 2 slide seat (25), and a movable seat (26) is slidably mounted on the two groups of No. 2 slide bars (241). A filling head (261) is mounted on the movable seat (26) via bolts, and the filling head (261) is connected to an external culture fluid supply device via a connecting pipe. A connecting wheel (242) is mounted on the lower end of the No. 1 slide seat (24) via a bearing, and a No. 3 motor (251) is mounted on the lower end of the No. 2 slide seat (25) via bolts. A belt pulley is mounted on the output shaft of the No. 3 motor (251), and a No. 2 transmission belt (252) is connected between the belt pulley on the output shaft of the No. 3 motor (251) and the connecting wheel (242), and the lower end of the movable seat (26) is fixedly connected to the No. 2 transmission belt (252).

6. The device for cultivating halophilic bacteria in a high-salt environment according to claim 5, characterized in that: A side plate (27) is fixed on the movable seat (26), two groups of No. 3 slide bars (271) are fixed on the side plate (27), a lifting plate (272) slides on the two groups of No. 3 slide bars (271), a hydraulic rod (273) is installed on the side plate (27) by bolts, a piston rod of the hydraulic rod (273) is connected to the lifting plate (272), a mounting seat (2721) is installed on the lifting plate (272) by bolts, and a detection head (2722) is fixed on the mounting seat (2721).

Images