Tissue dissociation device

Abstract

The utility model discloses a tissue dissociation device. The tissue dissociation device comprises a dissociation cavity, a rotating shaft and a collection cavity, the dissociation cavity is provided with an open first end and an open second end, and the open first end is detachably connected with the collection cavity; a second end of the opening is connected with the rotating shaft; the dissociation cavity is connected with the driving device; a lower flange of the dissociation cavity is matched with a test tube bayonet; the step surface at the lower part of the dissociation cavity is higher than the flange surface, so that the liquid in the dissociation cavity can be completely in a heating range, and a small amount of liquid can be heated; the dissociation cavity is a non-cylindrical surface, and one side of the dissociation cavity is an inner concave surface; one end of the collecting cavity is open, the other end of the collecting cavity is closed, step grinding teeth are arranged on the lower portion in the dissociation cavity, side wall grinding teeth are arranged on the inner wall of the dissociation cavity, and cutting blades are arranged on the step grinding teeth on the lower portion in the dissociation cavity. According to the tissue dissociation device provided by the utility model, the tissue can be cut and ground into uniform single-cell suspension without being cut into pieces manually, and the tissue dissociation device is convenient to operate and high in safety.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue dissociation, in particular to a tissue dissociation device. Background Art

[0002] As life science research enters the single-cell level, flow cytometry, cell typing detection, primary cell culture, single-cell sequencing and other technologies have gradually developed, all of which require high-quality cell suspensions as experimental materials. When preparing cell suspensions, fresh tissue samples are the primary condition for preparing cell suspensions. Among them, the tissue separation process is an important link and the primary link for the extraction of biomacromolecules. Tissue dissociation methods include enzymatic method, mechanical method and a combination of the two. Mechanical method is a more traditional tissue dissociation method, which is divided into mesh rubbing method and grinding method. Lymphatic tissue, such as spleen, lymph node, thymus and other samples, can be dissociated mechanically. Enzymatic method, according to the composition of tissue type, select the corresponding enzyme or enzyme combination for dissociation; the combination of the two is to add the corresponding enzyme for dissociation while mechanical dissociation. At present, when dissociating tissue, it is necessary to manually cut the tissue into pieces and put it into the dissociation device. This method relies entirely on the experience of the experimenter and is greatly affected by human operation factors.

[0003] In the prior art, a tissue processor and a stirring bottle thereof for biological tissue cutting are disclosed in the utility model with application number CN202320324034.0, wherein the stirring bottle comprises: a bottle body, a cover body and a cutting part connected to the cover body; the cover body comprises a fixed body and a rotating shaft joint rotatably connected to the center position of the fixed body; a conical structure gradually shrinking and extending along the axial direction is provided on the inner end face of the fixed body, and a fixed cutting blade extending along the axial direction is also provided on the inner end face of the fixed body; the cutting part comprises a rotating shaft and a spiral cutting blade, one end of the rotating shaft passes through the conical structure and is connected to the rotating shaft joint, and a sealing ring is provided at the joint between the rotating shaft and the conical structure to form a closed structure; one end of the spiral cutting blade is fixed on the rotating shaft, and the other end of the spiral cutting blade is a double-layer structure. When rotating, the fixed cutting blade can pass through the gap of the double-layer structure to form a shearing structure. The utility model can obtain a more stable cutting sample through the cutting blade structure, while improving the work efficiency.

[0004] However, although the above-mentioned prior art can improve the cutting effect to a certain extent, its crushing and grinding effect is not ideal for some tissues. In addition, in the above-mentioned prior art, the test tube body is open at one end and closed at the other end. During operation, the tissue and reagent need to be placed in the test tube body, tightened with a nut, and then installed upside down on the machine. This will produce two adverse effects. First, the tissue will hang on the wall of the test tube body, and it needs to be shaken repeatedly before the tissue will fall off; second, the nut and the test tube body are not tightened, which will cause leakage. In addition, the reagents are expensive. If leakage occurs during the experiment on the machine, it is very likely to damage the machine, which is easy to cause property losses. Utility Model Content

[0005] In view of the deficiencies in the prior art, the purpose of the utility model is to provide a tissue dissociation device, which can cut and grind tissues processed by the device into uniform single-cell suspensions without the need for manual shredding of the tissues, and is easy to operate and highly safe.

[0006] The technical solution adopted by the utility model to solve the technical problem is: a tissue dissociation device, including a dissociation chamber, a rotating shaft and a collection chamber; the dissociation chamber has an open first end and an open second end, and the open first end is detachably connected to the collection chamber; the open second end is connected to the rotating shaft; the dissociation chamber is connected to a driving device; the lower flange of the dissociation chamber cooperates with the test tube bayonet to achieve that the dissociation chamber does not rotate when the rotating shaft rotates; the step surface of the lower part of the dissociation chamber is higher than the flange surface, so that the liquid in the dissociation chamber can be completely within the heating range, thereby achieving the heating of a small amount of liquid; the dissociation chamber is a non-cylindrical surface, one side of which is an inner concave surface, so that the tissue will not float upward in a large area during dissociation; the collection chamber is open at one end and closed at the other end; the lower part of the dissociation chamber is provided with step grinding teeth, the inner wall of the dissociation chamber is provided with side wall grinding teeth, and the step grinding teeth at the lower part of the dissociation chamber are provided with cutting blades.

[0007] Furthermore, the rotating shaft is provided with a slot cooperating with the cutting blade; when the rotating shaft rotates, the cutting blade passes through the slot to cut the tissue; the rotating shaft includes at least one grinding surface; the grinding surface cooperates with the step grinding teeth to grind the tissue.

[0008] Furthermore, the step grinding teeth include at least one step row of teeth; the tooth shape direction of the step row of teeth is consistent with that of the side wall grinding teeth.

[0009] Furthermore, the step grinding teeth include three, which are step row of teeth one, step row of teeth two and step row of teeth three from bottom to top; the dissociation chamber includes at least one cutting blade; the cutting blade is arranged between step row of teeth one and step row of teeth two.

[0010] Furthermore, a spiral processing member is provided on the rotating shaft, the upper end of the spiral processing member is connected to the rotating shaft, and the lower end of the spiral processing member extends to the bottom of the step grinding tooth; the notch is opened on the spiral processing member.

[0011] Furthermore, the grinding surface is arranged on the inner side of the spiral processing piece, and the grinding surface includes three, which are grinding surface one, grinding surface two and grinding surface three from bottom to top; the grinding surface one, grinding surface two and grinding surface three are arranged corresponding to step tooth row one, step tooth row two and step tooth row three respectively.

[0012] Furthermore, the side wall grinding teeth are arranged at the middle and lower part of the inner wall of the dissociation chamber, and the setting height of the side wall grinding teeth is not lower than the upper end of the spiral processing element.

[0013] Furthermore, a seal is provided above the step grinding tooth where it connects with the rotating shaft.

[0014] Furthermore, the collection chamber is provided with a feature of being able to accommodate a filter screen, and the filter screen is detachable.

[0015] Furthermore, the collection chamber and the dissociation chamber are provided with matching sealing features, and the presence or absence of a filter does not affect the sealing of the collection chamber and the dissociation chamber.

[0016] The beneficial effects of the utility model are as follows: compared with the prior art, the tissue dissociation device provided by the utility model has the following advantages:

[0017] 1) The dissociation device provided by the utility model adopts a dissociation chamber with two openings at both ends. After one end of the dissociation chamber is connected to the rotating shaft, the tissue and reagent to be dissociated can be directly added from the other opening end. The tissue and reagent will directly reach the grinding surface without hanging on the wall. The dissociation device does not need to be inverted when placed on the machine, and there will be no leakage due to the nut not being tightened;

[0018] 2) The dissociation chamber in the dissociation device provided by the utility model adopts a non-circumferential concave design, which can form a rotating vortex and an internal rotating downward pressure force through the change of the inner wall profile to achieve a better grinding effect;

[0019] 3) The inner wall of the dissociation chamber in the dissociation device provided by the utility model is provided with side wall grinding teeth, which further improves the grinding efficiency;

[0020] 4) After the tissue is dissociated, the dissociation device is inverted to form a single cell suspension through the filtering effect of the filter. The filter is detachable and can be selected as needed; the skirt placement surface of the collection cavity is convenient for placement, and the conical structure of the closed end of the collection cavity is convenient for collecting trace single cell suspensions, without the need for additional collection containers, saving costs;

[0021] 5) The dissociation chamber in the utility model is a replaceable component, and different single cell suspensions can be prepared by configuring different dissociation chambers to change the clearance with the rotating shaft;

[0022] 6) The dissociation chamber is equipped with a cutting blade, which cooperates with the notch of the rotating shaft to cut the tissue into small pieces without manual cutting of the tissue; the grinding surface on the rotating shaft cooperates with the step grinding teeth on the dissociation chamber to fully grind the small pieces of tissue, improve the cell suspension effect, and have a high cell yield and high viability. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the three-dimensional structure of the dissociation device provided by the utility model.

[0024] Figure 2 This is a schematic diagram of the cross-sectional structure of the dissociation device provided by the utility model.

[0025] Figure 3 This is a schematic diagram of the cross-sectional structure of the dissociation chamber in the dissociation device provided by the utility model.

[0026] Figure 4 This is a schematic diagram of the connection structure between the dissociation chamber and the rotating shaft in the dissociation device provided by the utility model.

[0027] Among them: 1-collecting chamber; 11-filter screen; 12-skirt placement surface; 13-conical structure; 2-dissociation chamber; 21-step grinding teeth; 211-step row of teeth one; 212-step row of teeth two; 213-step row of teeth three; 22-side wall grinding teeth; 23-cutting blade; 24-seal; 25-step surface; 26-flange surface; 27-concave surface; 3-rotating axis; 31-grinding surface; 311-grinding surface one; 312-grinding surface two; 313-grinding surface three; 32-slot; 33-spiral processing part. DETAILED DESCRIPTION

[0028] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively with reference to the relevant drawings. The drawings provide preferred embodiments of the present invention. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the contents disclosed in the present invention.

[0029] Example

[0030] like Figures 1 to 4As shown, a tissue dissociation device comprises a dissociation chamber 2, a rotating shaft 3 and a collecting chamber 1; the dissociation chamber 2 has an open first end and an open second end, the open first end is detachably connected to the collecting chamber 1; the open second end is connected to the rotating shaft 3; the dissociation chamber 2 is connected to a driving device; the lower flange of the dissociation chamber 2 cooperates with the test tube bayonet to ensure that the dissociation chamber 2 does not rotate when the rotating shaft 3 rotates; the step surface 25 at the lower part of the dissociation chamber 2 is higher than the flange surface 26, so that the liquid in the dissociation chamber 2 can be completely within the heating range, thereby achieving heating of a small amount of liquid; the dissociation chamber 2 is a non-cylindrical surface, one side of which is an inner concave surface 27, so that the tissue will not float upward in a large area during dissociation; the lower part of the dissociation chamber 2 is provided with a step grinding tooth 21, the inner wall of the dissociation chamber 2 is provided with a side wall grinding tooth 22, and the step grinding tooth 21 at the lower part of the dissociation chamber 2 is provided with a cutting blade 23. The collection chamber 1 is open at one end and closed at the other end, and the closed end is a conical structure 13, and a skirt placement surface 12 is provided on the periphery of the conical structure 13. The skirt placement surface 12 facilitates the collection chamber 1 to be placed after being inverted, and the conical structure facilitates the collection of trace single cell suspensions. The collection chamber 1 can be used as a collection container to collect single cell suspensions, and no additional collection container is required, which saves costs.

[0031] In one embodiment, the rotating shaft 3 is provided with a slot 32 that cooperates with the cutting blade 23; when the rotating shaft 3 rotates, the cutting blade 23 passes through the slot 32 to cut the tissue; the rotating shaft 3 includes at least one grinding surface 31; the grinding surface 31 cooperates with the step grinding teeth 21 to grind the tissue.

[0032] In one embodiment, the step grinding teeth 21 include at least one step row of teeth; the step row of teeth and the side wall grinding teeth 22 have the same tooth shape direction. Preferably, the step grinding teeth 21 include three, from bottom to top, step row of teeth 1 211, step row of teeth 212 and step row of teeth 3 213; the dissociation chamber 2 includes at least one cutting blade 23; the cutting blade 23 is arranged between the step row of teeth 1 211 and the step row of teeth 212.

[0033] In one embodiment, a spiral processing member 33 is provided on the rotating shaft 3, the upper end of the spiral processing member 33 is connected to the rotating shaft 3, and the lower end of the spiral processing member 33 extends to the bottom of the step grinding tooth 21; the notch 32 is provided on the spiral processing member. When the rotating shaft 3 drives the spiral processing member 33 to rotate relative to the dissociation chamber 2 under the action of the driving device, the cutting blade 23 repeatedly passes through the notch 32 on the spiral processing member to achieve tissue cutting.

[0034] In one embodiment, the grinding surface 31 is arranged on the inner side of the spiral processing member 33, and the grinding surface 31 includes three, which are grinding surface 1 311, grinding surface 2 312 and grinding surface 313 from bottom to top; the grinding surface 1 311, grinding surface 2 312 and grinding surface 313 are arranged corresponding to step tooth row 1 211, step tooth row 212 and step tooth row 3 213 respectively.

[0035] In one embodiment, the side wall grinding teeth 22 are arranged at the middle and lower part of the inner wall of the dissociation chamber 2, and the setting height of the side wall grinding teeth 22 is not lower than the upper end of the spiral processing member 33. When the rotating shaft 3 rotates relative to the dissociation chamber 2 driven by the driving device, a grinding area for the tissue is formed between the side wall grinding teeth 22 and the spiral processing member 33, thereby enhancing the grinding effect.

[0036] In one embodiment, a seal 24 is provided above the step grinding teeth 21 where the step grinding teeth 21 meet the rotating shaft 3. The provision of the seal 24 strengthens the sealing performance of the connection between the rotating shaft 3 and the dissociation chamber 2 to prevent leakage.

[0037] In one embodiment, a filter screen 11 is provided in the collection chamber 1. The filter screen 11 is detachably provided near the connection between the dissociation chamber 2 and the collection chamber 1. When in use, it can be selected whether to install the filter screen according to actual needs. The filter screen 11 is used to filter the tissue fluid after dissociation. After filtering by the filter screen 11, a uniform single cell suspension can be collected in the collection chamber 1.

[0038] When the above-mentioned tissue dissociation device is used, the collection chamber 1 is first opened, the tissues and organs to be dissociated are placed in the dissociation chamber 2, and after the dissociation reagent is added, the collection chamber 1 is covered; the dissociation device is installed on the machine, and the lower flange of the dissociation chamber 2 cooperates with the test tube bayonet to achieve that the dissociation chamber 2 does not rotate when the rotating shaft 3 rotates; the rotating shaft 3 is driven by the matching driving device to rotate forward, reverse, high speed, and low speed to complete the chopping and grinding of the tissue, and finally form a single cell suspension, and the cell yield, that is, the number of cells per unit volume, and the cells are still alive and not dead are used as performance evaluation points. After the dissociation is completed, the dissociation device is inverted, and a single cell suspension can be formed through the filtering effect of the filter net 11, and the single cell suspension is collected in the collection chamber 1; the skirt placement surface 12 of the collection chamber 1 is convenient for placement, and the conical structure 13 at the closed end of the collection chamber 1 is used as a collection container to facilitate the collection of trace single cell suspensions.

[0039] The above implementation modes are only used to illustrate the present invention, but not to limit the present invention. Ordinary technicians in the relevant technical field can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention. The scope of patent protection of the present invention should be limited by the claims.

Claims

1. A tissue dissociation device, comprising a dissociation chamber, a rotating shaft and a collection chamber; characterized in that: The dissociation chamber has an open first end and an open second end, and the open first end is detachably connected to the collection chamber; the open second end is connected to the rotating shaft; the dissociation chamber is connected to a driving device; the lower flange of the dissociation chamber cooperates with the test tube bayonet; the step surface at the lower part of the dissociation chamber is higher than the flange surface, so that all the liquid in the dissociation chamber can be within the heating range, thereby achieving the heating of a small amount of liquid; the dissociation chamber is a non-cylindrical surface, one side of which is an inner concave surface; the collection chamber is open at one end and closed at the other end; the lower part of the dissociation chamber is provided with step grinding teeth, the inner wall of the dissociation chamber is provided with side wall grinding teeth, and the step grinding teeth at the lower part of the dissociation chamber are provided with cutting blades.

2. A tissue dissociation device as claimed in claim 1, characterized in that: The rotating shaft is provided with a notch which cooperates with the cutting blade; when the rotating shaft rotates, the cutting blade passes through the notch to cut the tissue; the rotating shaft includes at least one grinding surface; the grinding surface cooperates with the step grinding teeth to grind the tissue.

3. A tissue dissociation device as claimed in claim 1, characterized in that: The step grinding teeth include at least one step row of teeth; the tooth shape direction of the step row of teeth is consistent with that of the side wall grinding teeth.

4. A tissue dissociation device as claimed in claim 3, characterized in that: The step grinding teeth include three, which are step row teeth one, step row teeth two and step row teeth three from bottom to top; the dissociation chamber includes at least one cutting blade; the cutting blade is arranged between the step row teeth one and the step row teeth two.

5. A tissue dissociation device as claimed in claim 1, characterized in that: A spiral processing piece is arranged on the rotating shaft, the upper end of the spiral processing piece is connected to the rotating shaft, and the lower end of the spiral processing piece extends to the bottom of the step grinding tooth; a notch is arranged on the spiral processing piece.

6. A tissue dissociation device as claimed in claim 2, characterized in that: The grinding surface is arranged on the inner side of the spiral processing element, and the grinding surface includes three, which are grinding surface one, grinding surface two and grinding surface three from bottom to top; the grinding surface one, grinding surface two and grinding surface three are arranged corresponding to step tooth row one, step tooth row two and step tooth row three respectively.

7. A tissue dissociation device as claimed in claim 1, characterized in that: The side wall grinding teeth are arranged at the middle and lower part of the inner wall of the dissociation chamber, and the setting height of the side wall grinding teeth is not lower than the upper end of the spiral processing element.

8. A tissue dissociation device as claimed in claim 1, characterized in that: A sealing member is provided at the joint between the step grinding teeth and the rotating shaft.

9. A tissue dissociation device as claimed in claim 1, characterized in that: The collection chamber is provided with a feature in which a filter screen can be installed, and the filter screen is detachable.

10. A tissue dissociation device according to claim 1 or 9, characterized in that: The collection chamber and the dissociation chamber are provided with matching sealing features, and the presence or absence of a filter screen does not affect the sealing of the collection chamber and the dissociation chamber.

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