Glioma stem cell cryopreservation device

Abstract

The utility model belongs to the technical field of cell cryopreservation, and particularly relates to a glioma stem cell cryopreservation device which comprises a cryopreservation tube body, the cryopreservation tube body is a hollow cylinder with an upward opening, a base is arranged at the bottom of the cryopreservation tube body, a plurality of first mounting grooves are formed in the side wall of the base, and rotating shafts are rotationally mounted on the inner walls of the first mounting grooves; a supporting leg is fixed to the outer wall of the rotating shaft, torsional springs are arranged on the outer wall of the rotating shaft and located on the two sides of the supporting leg, the two ends of each torsional spring are fixed to the outer wall of the supporting leg and the inner wall of the first mounting groove correspondingly, and a pipe cap is connected to the top of the cryopreservation pipe body in a buckled mode. A balancing weight is embedded in the bottom of the base; according to the glioma stem cell cryopreservation tube box, a cryopreservation tube can be conveniently and stably placed, the toppling risk is avoided, the sealing performance is greatly improved, glioma stem cells can be better stored, and the safety and effectiveness of the glioma stem cells in the cryopreservation process are guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell cryopreservation, and specifically relates to a glioma stem cell cryopreservation device. Background Art

[0002] Glioma stem cells are a type of cells with stem cell characteristics in glioma tissues. Glioma stem cells play an important role in the occurrence and development of gliomas. They have the ability to induce tumor angiogenesis, promote tumor invasion and spread, and can quickly rebuild tumors under the pressure of conventional treatment, leading to rapid recurrence of gliomas. Therefore, research on glioma stem cells is of great significance for the treatment of gliomas; glioma stem cells are generally cryopreserved through tissue cryopreservation tubes. Cryopreservation tubes are laboratory consumables specially used to store glioma stem cells, which can be cryopreserved in a gaseous state of liquid nitrogen.

[0003] At present, the cryopreservation tubes used in glioma stem cell banks have problems with single structure and poor sealing performance. These cryopreservation tubes are simply designed and often lack sufficient stability. Their light weight and compactness make them easy to tip over, which is not conducive to stable placement. Utility Model Content

[0004] The purpose of the present invention is to provide a glioma stem cell cryopreservation device, which can conveniently and stably place cryopreservation tubes, avoid the risk of tipping over, and greatly enhance the sealing performance, which is conducive to better storage of glioma stem cells and ensures their safety and effectiveness during the cryopreservation process.

[0005] The technical solutions adopted in this utility are as follows:

[0006] A glioma stem cell cryopreservation device comprises a cryopreservation tube body, which is a hollow cylinder with an opening facing upward. A base is provided at the bottom of the cryopreservation tube body, and a plurality of first mounting grooves are provided on the side wall of the base. A rotating shaft is rotatably mounted on the inner wall of the first mounting groove, and a support leg is fixed to the outer wall of the rotating shaft. Torsion springs are provided on the outer wall of the rotating shaft and on both sides of the support leg, and the two ends of the torsion spring are respectively fixed to the outer wall of the support leg and the inner wall of the first mounting groove. A tube cap is connected by a buckle on the top of the cryopreservation tube body.

[0007] Furthermore, a counterweight is embedded in the bottom of the base.

[0008] Furthermore, a plurality of plug-in blocks are fixed on the inner side of the tube cap, an L-shaped plug-in groove is provided on the outer wall of the cryopreservation tube body corresponding to the plug-in blocks, a first sealing ring is fixed on the inner top wall of the tube cap, and a second sealing ring is fixed on the top of the cryopreservation tube body.

[0009] Furthermore, a clamping block is fixed on the top of the plug-in block, and a clamping slot cooperating with the clamping block is formed on the inner wall of the L-shaped plug-in slot.

[0010] Furthermore, the first sealing ring and the second sealing ring are both made of silicone rubber material.

[0011] Furthermore, limiting blocks are fixed on both sides of the supporting leg, and the inner wall of the first installation groove is provided with a limiting block slidably connected to the limiting block.

[0012] The technical effects achieved by this utility model are:

[0013] The practical glioma stem cell freezing device is provided with supporting legs, which cooperate with torsion springs. After being separated from the external placement hole, the supporting legs can be unfolded, so that the freezing tube can be placed stably and conveniently, avoiding the risk of tipping over. During the insertion into the placement hole, the supporting legs will be retracted into the first installation groove, so that the freezing tube can be placed in the external test tube box conveniently, showing its high practicality and perfection. The provided cover cap is convenient for connecting with the sealing buckle of the freezing tube body, and also greatly enhances the sealing performance, which is conducive to better storage of glioma stem cells and ensures their safety and effectiveness during the freezing process. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0015] Figure 2 It is a side-view stereoscopic structural schematic diagram of the utility model;

[0016] Figure 3 This is a practical Figure 1 Schematic diagram of the enlarged structure of area A in the middle.

[0017] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0018] 1. Cryotube body; 2. Base; 3. First installation slot; 4. Rotating shaft; 5. Support foot; 6. Torsion spring; 7. Limiting slot; 8. Limiting block; 9. Counterweight block; 10. Tube cap; 11. First sealing ring; 12. Plug-in block; 13. L-shaped plug-in slot; 14. Second sealing ring; 15. Card slot; 16. Card block. DETAILED DESCRIPTION

[0019] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is specifically described below in conjunction with the embodiments. It should be understood that the following text is only used to describe one or several specific implementations of the present invention, and does not strictly limit the scope of protection specifically requested by the present invention.

[0020] like Figure 1-3As shown, the glioma stem cell freezing device includes a freezing tube body 1, which is a hollow cylinder with an opening facing upward. A base 2 is provided at the bottom of the freezing tube body 1, and a plurality of first mounting grooves 3 are provided on the side wall of the base 2. A rotating shaft 4 is rotatably installed on the inner wall of the first mounting groove 3, and a support leg 5 is fixed on the outer wall of the rotating shaft 4, so as to stably support the freezing tube body 1 and prevent it from tipping over. A torsion spring 6 is provided on the outer wall of the rotating shaft 4 and on both sides of the support leg 5, and the two ends of the torsion spring 6 are respectively fixed to the outer wall of the support leg 5 and the inner wall of the first mounting groove 3. When the support leg 5 contacts the placement hole in the external test tube box, , the support leg 5 can be smoothly rotated into the first installation groove 3 for storage, so that the cryopreservation tube body 1 can be smoothly inserted into the placement hole, and the top buckle of the cryopreservation tube body 1 is connected with a tube cap 10, which has a simple structure and is easy to operate. Through the setting of the support leg 5, the cryopreservation tube body 1 can be stably placed; a counterweight block 9 is inlaid at the bottom of the base 2 to further stably place the cryopreservation tube body 1; both sides of the support leg 5 are fixed with a limiting block 8, and the inner wall of the first installation groove 3 is provided with a limiting block 8 slidably connected to the limiting block 8, which is convenient for the rotation of the support leg 5 to limit the position, so that the support leg 5 can normally support the cryopreservation tube body 1;

[0021] like Figure 1-2 As shown, a plurality of plug-in blocks 12 are fixed on the inner side of the tube cap 10, an L-shaped plug-in groove 13 is provided on the outer wall of the cryopreservation tube body 1 corresponding to the plug-in block 12, a first sealing ring 11 is fixed on the inner top wall of the tube cap 10, and a second sealing ring 14 is fixed on the top of the cryopreservation tube body 1, which is convenient for sealing, fixing and disassembling the tube cap 10 and the cryopreservation tube body 1, which is convenient and labor-saving; a clamping block 16 is fixed on the top of the plug-in block 12, and a clamping groove 15 matching the clamping block 16 is provided on the inner wall of the L-shaped plug-in groove 13. After the plug-in block 12 is inserted into the L-shaped plug-in groove 13, the clamping block 16 is inserted into the L-shaped plug-in groove 13. 16 is inserted into the card slot 15, which further improves the sealing performance of the connection between the tube cap 10 and the cryopreservation tube body 1; the first sealing ring 11 and the second sealing ring 14 are both made of silicone rubber material, which further increases the sealing performance of the connection between the cryopreservation tube body 1 and the tube cap 10, and can also increase the friction between the cryopreservation tube body 1 and the tube cap 10. In addition, the first sealing ring 11 and the second sealing ring 14 have a certain elasticity. When the tube cap 10 is covered, the tube cap 10 will have an upward force, which, in conjunction with the L-shaped plug-in groove 13 and the plug-in block 12, improves the stability of the fixation of the tube cap 10.

[0022] The working principle of the utility model is as follows: when in use, after the cryopreservation tube body 1 is taken out from the external test tube box, the cryopreservation tube body 1 is separated from the placement hole. Because of the torsion force of the torsion spring 6, the support leg 5 is driven to rotate, and the upper limit block 8 and the limiting groove 7 are cooperated to limit the rotation of the support leg 5, so that one end of the support leg 5 is rotated out of the first installation groove 3, and the cryopreservation tube body 1 is stably supported, so that the cryopreservation tube body 1 is not easy to tip over. The counterweight block 9 is provided to increase the bottom weight of the cryopreservation tube body 1 so that the cryopreservation tube body 1 can be placed stably; when the provided pipe cap 10 is connected with the cryopreservation tube body 1, the plug-in block 12 on the pipe cap 10 is aligned with the vertical groove of the L-shaped plug-in groove 13 and inserted into it, the pipe cap 10 is pressed and rotated, and the pipe cap 10 drives the plug-in block 12 to rotate along one side of the L-shaped plug-in groove 13 until the clamping block 16 is clamped into the clamping groove 15, and the provided first sealing ring 11 and the second sealing ring 14 increase the sealing and stability of the connection between the cryopreservation tube body 1 and the pipe cap 10.

[0023] The above is only a preferred embodiment of the present invention. It should be noted that, for ordinary technicians in the field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A glioma stem cell cryopreservation device, characterized in that: The invention comprises a cryopreservation tube body (1), wherein the cryopreservation tube body (1) is a hollow cylinder with an opening facing upward, a base (2) is arranged at the bottom of the cryopreservation tube body (1), a plurality of first mounting grooves (3) are arranged on the side wall of the base (2), a rotating shaft (4) is rotatably mounted on the inner wall of the first mounting groove (3), a support leg (5) is fixed on the outer wall of the rotating shaft (4), a torsion spring (6) is arranged on the outer wall of the rotating shaft (4) and on both sides of the support leg (5), and the two ends of the torsion spring (6) are respectively fixed to the outer wall of the support leg (5) and the inner wall of the first mounting groove (3), and a tube cap (10) is buckled and connected to the top of the cryopreservation tube body (1).

2. The glioma stem cell cryopreservation device according to claim 1, characterized in that: A counterweight block (9) is embedded at the bottom of the base (2).

3. The glioma stem cell cryopreservation device according to claim 1, characterized in that: A plurality of plug-in blocks (12) are fixed on the inner side of the tube cap (10); an L-shaped plug-in groove (13) is provided on the outer wall of the cryopreservation tube body (1) corresponding to the plug-in blocks (12); a first sealing ring (11) is fixed on the inner top wall of the tube cap (10); and a second sealing ring (14) is fixed on the top of the cryopreservation tube body (1).

4. The glioma stem cell cryopreservation device according to claim 3, characterized in that: A clamping block (16) is fixed on the top of the plug-in block (12), and a clamping groove (15) matching with the clamping block (16) is formed on the inner wall of the L-shaped plug-in groove (13).

5. The glioma stem cell cryopreservation device according to claim 3, characterized in that: The first sealing ring (11) and the second sealing ring (14) are both made of silicone rubber material.

6. The glioma stem cell cryopreservation device according to claim 1, characterized in that: Limiting blocks (8) are fixed on both sides of the supporting foot (5), and the inner wall of the first mounting groove (3) is provided with a limiting block (8) slidably connected to the limiting block (8).

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