Optimized cell exosome culture device
By designing an optimized culture device for cell exosomes that are easy to observe and regulate, the problems of inaccurate control of cell growth environment and insufficient constant temperature storage in the prior art are solved, and efficient cell exosome yield and sample storage stability are achieved.
Patent Information
- Application Number
- CN202420714153.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-09
- Publication Date
- 2025-03-14
- Estimated Expiration
- 2034-04-09
AI Technical Summary
The existing cell exosome optimization culture device has insufficient accuracy and stability in controlling the accuracy and stability of cell growth conditions, and it is prone to disturb the cell growth environment during use, lacking sufficient constant temperature storage capacity, limiting the researchers' ability to process large numbers of samples.
An optimized culture device for cell exosomes including a bottom box, a working plate and an upper plate is designed, which has the function of easy observation and regulation. Through the visual glass window and operating panel, the environmental parameters in the incubator can be monitored and adjusted in real time; at the same time, the device is equipped with a thermostat and a ventilator to ensure constant temperature storage and environmental purity.
Accurate control of the cell growth environment is achieved, the uncertainty of experimental results is reduced, the yield and quality of cell exosomes are improved, the stable preservation of samples is ensured, and the accuracy and efficiency of experiments are improved.
Smart Images
Figure CN222613355U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, and specifically refers to a cell exosome optimization culture device. Background Art
[0002] The cell exosome optimization culture device is a device for producing and collecting cell exosomes. Cell exosomes are a type of tiny extracellular vesicles that are secreted by cells outside the cell and contain cell components such as proteins, nucleic acids and lipids. These cell exosomes are believed to play an important role in intercellular communication, signal transduction and disease occurrence.
[0003] At present, there are some challenges in regulating the cell growth environment of cell exosome optimization culture devices. First, some devices may not be ideal in controlling the accuracy and stability of cell growth conditions. For example, the regulation of parameters such as temperature, pH value and oxygen concentration may not be accurate enough, which may affect the yield and quality of cell exosomes. Secondly, some devices need to be opened for observation during use, which may cause temporary disturbances in the cell growth environment, thereby affecting the accuracy of the experimental results. In addition, current cell exosome culture devices also have certain limitations in storage capacity, and usually lack sufficient constant temperature storage and culture areas, which may limit the ability of researchers to process a large number of samples within a certain period of time. Utility Model Content
[0004] In order to solve the above-mentioned problems, the utility model proposes a cell exosome optimization culture device which is convenient for observing the situation in the incubator, convenient for adjusting the parameters in the incubator, and capable of constant temperature storage.
[0005] In order to solve the above technical problems, the technical solution proposed in the utility model is: a cell exosome optimization culture device, comprising a bottom box and a working plate, an upper plate is provided above the bottom box, a temporary storage structure for temporarily storing cell samples is provided between the upper plate and the bottom box, incubators are evenly distributed on the upper plate, a culture environment adjustment structure is provided in the incubator, an upper cover is covered on the incubator, a thermostat is connected to one side of the bottom box, a ventilator is connected to the other side of the bottom box, and visual glass windows are provided on the upper cover and on one side of the incubator.
[0006] Furthermore, the temporary storage structure includes an interlayer connected to the work plate, and a drawer is slidably provided in the interlayer.
[0007] Furthermore, a round handle is connected to the middle of the front of the drawer.
[0008] Furthermore, the culture environment adjustment structure includes a placement dish connected to the middle of the culture box, an ultraviolet lamp is provided at the upper end of one side of the culture box, a lighting lamp is provided below the ultraviolet lamp, a heater is provided at the upper end of the other side of the culture box, and a temperature and humidity display is connected to the bottom of the culture box.
[0009] Furthermore, an operation panel is connected to the upper plate and close to the incubator.
[0010] Furthermore, partition layers are equidistantly arranged in the bottom box, a closed door is rotatably arranged at the front of the bottom box, and a closed lock for matching use is arranged in the middle of the closed door.
[0011] Compared with the prior art, the utility model has the following advantages:
[0012] Easy to observe the situation inside the incubator: Through the visual glass windows on the upper cover and the side of the incubator, you can directly observe the cell samples and culture environment in the incubator without opening the box to avoid disturbing the internal environment. The control functions on the operation panel, such as the control of the lighting and heater, can be adjusted according to actual needs, which is conducive to adjusting the light and temperature in the incubator.
[0013] Easy to adjust the parameters in the incubator: The temperature and humidity display connected to the bottom can monitor the temperature and humidity in the incubator in real time, making it easy to adjust the temperature and humidity parameters in time to ensure a good growth environment for cell samples. By controlling the thermostat, the culture temperature in the bottom box can be accurately controlled to maintain a constant temperature condition to meet the culture requirements of different cell samples.
[0014] Advantages of constant temperature storage: The control of the thermostat can ensure the stability of the temperature in the incubator, and the growth and activity of cells will not be affected by temperature fluctuations during the cell culture process. The ventilator can ventilate the bottom box to prevent the internal cell samples from being contaminated and ensure the purity of the culture environment. The interlayer allows the device to meet the needs of large-scale constant temperature culture and non-constant temperature culture at the same time, with high flexibility and practicality. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a three-dimensional diagram of the utility model;
[0016] Figure 2 It is the front view of the utility model;
[0017] Figure 3 It is a left view of the utility model;
[0018] Figure 4 It is a top view of the utility model.
[0019] As shown in the figure: 1. Work board; 2. Upper board; 3. Interlayer; 4. Drawer; 5. Round handle; 6. Incubator; 7. Upper cover; 8. Visual glass window; 9. Operation panel; 10. Ultraviolet lamp; 11. Lighting lamp; 12. Heater; 13. Temperature and humidity display; 14. Placement dish; 15. Bottom box; 16. Closed door; 17. Closed lock; 18. Partition layer; 19. Thermostat; 20. Ventilator. DETAILED DESCRIPTION
[0020] The cell exosome optimization culture device with convenient observation and adjustment functions and constant temperature storage has the following advantages: real-time monitoring of the environment in the incubator to ensure stable cell growth; easy adjustment of parameters to optimize the yield and quality of cell exosomes; constant temperature storage function can ensure stable sample storage and facilitate subsequent experiments; improve experimental accuracy and efficiency, and promote the research and application of cell exosomes. The utility model is further described in detail below in conjunction with the accompanying drawings.
[0021] Combined with Figure 2 An upper plate 2 is provided above the bottom box 15, and a temporary storage structure for temporarily storing cell samples is provided between the upper plate 2 and the bottom box 15. The temporary storage structure includes an interlayer 3 connected to the working plate 1, a drawer 4 is slidably provided in the interlayer 3, a round handle 5 is connected to the middle of the front of the drawer 4, and an operation panel 9 is connected to the upper plate 2 and close to the incubator 6.
[0022] Combined with Figure 1 The upper plate 2 is evenly distributed with incubators 6, and a culture environment regulating structure is provided in the incubator 6. The culture environment regulating structure includes a placement dish 14 connected to the middle of the incubator 6, an ultraviolet lamp 10 is provided at the upper end of one side of the incubator 6, and a lighting lamp 11 is provided below the ultraviolet lamp 10. A heater 12 is provided at the upper end of the other side of the incubator 6, and a temperature and humidity display 13 is connected to the bottom surface of the incubator 6.
[0023] Combined with Figure 3 The incubator 6 is covered with an upper cover 7, a thermostat 19 is connected to one side of the bottom box 15, a ventilator 20 is connected to the other side of the bottom box 15, and visual glass windows 8 are provided on the upper cover 7 and on one side of the incubator 6. Partition layers 18 are equidistantly provided in the bottom box 15, and a closed door 16 is rotatably provided in front of the bottom box 15, and a closed lock 17 is provided in the middle of the closed door 16 for use with the bottom box 15.
[0024] Specific implementation methods of the utility model: When the utility model is in use, the cell samples that need to be cultured at a constant temperature in large quantities are placed on the partition plate in the bottom box 15, the closed door 16 is closed, and the closed lock 17 is locked. The culture temperature in the bottom box 15 is controlled by controlling the thermostat 19. The bottom box 15 can be ventilated by the ventilator 20 to prevent contamination of the internal cell samples. Some cell samples that do not need constant temperature culture can be placed in the drawer 4 in the interlayer 3. When cell extrasome optimization culture is required, they are placed in the placement dish 14 in the incubator 6. The samples can be observed through the upper cover 7 and the visible glass window 8 on one side of the incubator 6. The lighting lamp 11 can be controlled by the operation panel 9 for lighting, the heater 12 can be controlled for heating, and the temperature and humidity in the incubator 6 can be displayed by the temperature and humidity display 13. When not in use, the ultraviolet lamp 10 can be turned on to disinfect the incubator 6, and the door can be closed for disinfection for half an hour and then returned to close.
[0025] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0026] The above description of the utility model and its implementation methods is not restrictive. The drawings show only one implementation method of the utility model, and the actual structure is not limited thereto. In short, if ordinary technicians in this field are inspired by it and design structural methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention of the utility model, they should all fall within the protection scope of the utility model.
Claims
1. A cell exosome optimization culture device, comprising a bottom box (15) and a working plate (1), characterized in that: An upper plate (2) is provided above the bottom box (15); a temporary storage structure for temporarily storing cell samples is provided between the upper plate (2) and the bottom box (15); incubators (6) are evenly distributed on the upper plate (2); a culture environment regulating structure is provided inside the incubators (6); an upper cover (7) is provided on the incubator (6); a thermostat (19) is connected to one side of the bottom box (15); a ventilator (20) is connected to the other side of the bottom box (15); and visual glass windows (8) are provided on the upper cover (7) and on one side of the incubator (6).
2. The device for optimizing the culture of exosomes according to claim 1, characterized in that: The temporary storage structure comprises an interlayer (3) connected to the working board (1), and a drawer (4) is slidably arranged in the interlayer (3).
3. The device for optimizing the culture of exosomes according to claim 2, characterized in that: A round handle (5) is connected to the middle of the front of the drawer (4).
4. The device for optimizing the culture of exosomes according to claim 1, characterized in that: The culture environment adjustment structure comprises a placement dish (14) connected to the middle of the culture box (6); an ultraviolet lamp (10) is provided at the upper end of one side of the culture box (6); a lighting lamp (11) is provided below the ultraviolet lamp (10); a heater (12) is provided at the upper end of the other side of the culture box (6); and a temperature and humidity display (13) is connected to the bottom surface of the culture box (6).
5. The device for optimizing the culture of exosomes according to claim 4, characterized in that: An operation panel (9) is connected to the upper plate (2) and is close to the incubator (6).
6. The device for optimizing the culture of exosomes according to claim 1, characterized in that: The bottom box (15) has partition layers (18) arranged at equal intervals in the bottom box (15). A closed door (16) is rotatably arranged at the front of the bottom box (15). A closed lock (17) for matching is arranged in the middle of the closed door (16).