Reaction tank for nucleic acid preparation and amplification

Abstract

The utility model discloses a reaction tank for nucleic acid preparation and amplification, relates to the technical field of nucleic acid amplification, and solves the technical problem that in the existing technical scheme, the temperature cannot be integrally and quickly adjusted and the annealing temperature cannot be accurately controlled. The storage box is internally and fixedly provided with an isolation frame, the isolation frame is internally and fixedly provided with a main tank body, the main tank body is internally and fixedly provided with a test tube rack, and the test tube rack is internally and fixedly provided with a cultivation tube. A user can accurately control the reaction temperature in a short time, and particularly, the adjustment of the annealing temperature is more flexible, so that the experiment efficiency is effectively improved; the built-in auxiliary heating pipe and the built-in water pump machine ensure the uniformity and stability of the temperature in the reaction process, enhance the repeatability and reliability of nucleic acid amplification, and are particularly suitable for experiments sensitive to the temperature.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid amplification, in particular to a reaction tank used for nucleic acid preparation and amplification. Background Art

[0002] The technical field to which the utility model relates is that the reaction tank for nucleic acid preparation and amplification is a specially designed device, which is mainly used for amplifying and processing DNA or RNA. Its core functions include temperature control, mixing and stirring, pressure control and convenient operation. The reaction tank can accurately control the temperature to ensure temperature stability during the reaction process, thereby improving the amplification efficiency and ensuring the quality of the nucleic acid. At the same time, the built-in stirring mechanism can evenly mix the reaction solution, promote full contact of the components, and ensure the smooth progress of the reaction. In addition, the reaction tank is also provided with a pressure release valve to prevent accidents in a high temperature and high pressure environment, thereby ensuring the safety of the experiment.

[0003] This equipment is of great significance in biomedical research and applications. By optimizing the reaction conditions, the reaction tank significantly improves the efficiency of nucleic acid amplification, ensures the reliability of the results, and enables biological research such as gene cloning, gene expression analysis, and mutation detection to proceed smoothly. At the same time, it is widely used in medical testing, forensic medicine, and pathogen detection, improving the efficiency of diagnosis and research. In addition, with the advancement of technology, the research and development of reaction tanks has also promoted the emergence of new amplification technologies, providing more possibilities for scientific exploration. Therefore, the reaction tank for nucleic acid preparation and amplification is not only a key equipment in the laboratory, but also an important tool for the development of modern biological sciences.

[0004] The existing technical solutions have the technical problem of being unable to quickly adjust the temperature in an integrated manner to accurately control the annealing temperature. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a reaction tank for nucleic acid preparation and amplification, which solves the technical problem that the prior art solution cannot quickly adjust the temperature in an integrated manner and accurately control the annealing temperature.

[0006] To achieve the above objectives, the utility model is implemented through the following technical solutions: a reaction tank for nucleic acid preparation and amplification, comprising a main frame, a reserve box is fixedly installed on the wall of the main frame, an isolation frame is fixedly installed in the reserve box, a main tank body is fixedly installed in the isolation frame, a test tube rack is fixedly installed in the main tank body, a culture tube is fixedly installed in the test tube rack, an auxiliary stirring structure is installed in the main tank body, and a water bath temperature control structure is installed in the main tank body.

[0007] Preferably, a tank cover is rotatably mounted on the upper wall of the main tank body, an automatic lock is fixedly mounted on the other end of the tank cover, an electric push rod is rotatably mounted on the upper end of the storage box, and the telescopic end of the electric push rod is rotatably mounted on the side wall of the tank cover.

[0008] Preferably, the auxiliary stirring structure includes a stirring frame, which is fixedly installed on the upper end of the main tank body and located on the inner side of the tank body cover. A stirring motor is fixedly installed in the stirring frame, a stirring shaft is fixedly installed at the driving end of the stirring motor, and a stirring head is fixedly installed on the stirring shaft.

[0009] Preferably, the water bath temperature control structure includes a water storage tank, a water bath cavity is opened in the main tank body, the water storage tank is fixedly installed in the reserve tank, a water pump is fixedly installed in the water storage tank, the output end of the water pump is fixedly connected to the main tank body, a drain pipe is fixedly installed on the water bath cavity, and the drain pipe is fixedly connected to the water storage tank.

[0010] Preferably, an auxiliary heating tube is fixedly installed in the water bath chamber, and the auxiliary heating tube is a resistance electric heating spiral tube.

[0011] Preferably, a semiconductor refrigeration plate is fixedly mounted on the rear wall of the water tank, a cold end of the semiconductor refrigeration plate is fixedly mounted on the side wall of the water tank, a heat sink is fixedly mounted on the hot end of the semiconductor refrigeration plate, and an air supply fan is fixedly mounted inside the heat sink.

[0012] Preferably, a storage battery is fixedly installed in the reserve box, an operation panel is fixedly installed at the front end of the reserve box, and a support column is fixedly installed at the lower end of the main frame. Beneficial Effects

[0013] The utility model provides a reaction tank for nucleic acid preparation and amplification. The utility model realizes rapid temperature adjustment through the setting of a water bath temperature adjustment structure and a semiconductor refrigeration plate. The user can accurately control the reaction temperature in a short time, especially more flexibly in adjusting the annealing temperature, and effectively improves the efficiency of the experiment; the built-in auxiliary heating tube and water pump ensure the uniformity and stability of the temperature during the reaction process, enhance the repeatability and reliability of nucleic acid amplification, and is particularly suitable for experiments that are sensitive to temperature; the design of the auxiliary stirring structure enables the reaction liquid to be fully mixed, optimizes the reaction conditions, improves the amplification efficiency, and ensures the uniform distribution of the reaction components; the automatic locking structure and electric push rod design of the reaction tank improve the safety and convenience of the equipment, and the user can easily open and close the tank cover, reducing the risk of accidents during operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the main cross-sectional structure of a reaction tank for nucleic acid preparation and amplification described in the utility model.

[0015] In the figure: 1. main frame; 2. storage tank; 3. isolation rack; 4. main tank; 5. test tube rack; 6. culture tube; 7. tank cover; 8. automatic lock; 9. electric push rod; 10. stirring rack; 11. stirring motor; 12. stirring shaft; 13. stirring head; 14. water storage tank; 15. water bath chamber; 16. water pump; 17. drainage pipe; 18. auxiliary heating pipe; 19. semiconductor cooling chip; 20. heat sink; 21. air supply fan; 22. battery; 23. operation panel; 24. support column; DETAILED DESCRIPTION

[0016] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined purpose of the utility model, the technical scheme in the embodiment of the utility model will be clearly and completely described below in combination with the drawings in the embodiment of the utility model. Obviously, the described embodiment is only a part of the embodiment of the utility model, rather than all the embodiments. The detailed description is as follows.

[0017] See also Figure 1 The utility model provides a technical solution: a reaction tank for nucleic acid preparation and amplification, comprising a main frame 1, a reserve box 2 is fixedly installed on the upper wall of the main frame 1, an isolation frame 3 is fixedly installed in the reserve box 2, a main tank body 4 is fixedly installed in the isolation frame 3, a test tube rack 5 is fixedly installed in the main tank body 4, a culture tube 6 is fixedly installed in the test tube rack 5, an auxiliary stirring structure is installed in the main tank body 4, and a water bath temperature control structure is installed in the main tank body 4.

[0018] This embodiment is further configured such that a tank cover 7 is rotatably mounted on the upper wall of the main tank body 4, an automatic lock 8 is fixedly mounted on the other end of the tank cover 7, an electric push rod 9 is rotatably mounted on the upper end of the storage box 2, and the telescopic end of the electric push rod 9 is rotatably mounted on the side wall of the tank cover 7.

[0019] The present embodiment is further configured such that the auxiliary stirring structure includes a stirring frame 10, the stirring frame 10 is fixedly mounted on the upper end of the main tank body 4 and located on the inner side of the tank body cover 7, a stirring motor 11 is fixedly mounted in the stirring frame 10, a stirring shaft 12 is fixedly mounted on the driving end of the stirring motor 11, and a stirring head 13 is fixedly mounted on the stirring shaft 12.

[0020] This embodiment is further configured as follows: the water bath temperature control structure includes a water storage tank 14, a water bath chamber 15 is opened in the main tank body 4, the water storage tank 14 is fixedly installed in the reserve tank 2, a water pump 16 is fixedly installed in the water storage tank 14, the output end of the water pump 16 is fixedly connected to the main tank body 4, a drain pipe 17 is fixedly installed on the water bath chamber 15, and the drain pipe 17 is fixedly connected to the water storage tank 14.

[0021] The present embodiment is further configured such that an auxiliary heating tube 18 is fixedly installed in the water bath chamber 15 , and the auxiliary heating tube 18 is a resistance electric heating spiral tube.

[0022] The present embodiment is further configured such that a semiconductor refrigeration sheet 19 is fixedly mounted on the rear wall of the water storage tank 14 , a cold end of the semiconductor refrigeration sheet 19 is fixedly mounted on the side wall of the water storage tank 14 , a heat sink 20 is fixedly mounted on the hot end of the semiconductor refrigeration sheet 19 , and an air supply fan 21 is fixedly mounted inside the heat sink 20 .

[0023] This embodiment is further configured such that a storage battery 22 is fixedly installed in the reserve box 2 , an operation panel 23 is fixedly installed at the front end of the reserve box 2 , and a support column 24 is fixedly installed at the lower end of the main frame 1 .

[0024] The detailed connection means are well known in the art; Figure 1 As shown,

[0025] Ensure that all components of the reaction tank are intact, especially the power supply, water storage tank 14 and stirring motor 11. Check whether enough water is added to the water bath chamber 15.

[0026] Prepare the nucleic acid samples (such as DNA or RNA), primers, polymerase and other necessary reagents to be amplified according to experimental requirements, and ensure that they are kept in appropriate storage conditions before use.

[0027] Place the test tube rack 5 in the main tank 4, and fix the culture tube 6 on the test tube rack 5. Make sure that the tube is stable and will not move or fall over during stirring.

[0028] Connect the power supply of the reserve tank 2 and check whether the battery 22 is sufficiently charged. Make sure that the main frame 1 is placed on a stable work surface.

[0029] Set the required reaction temperature and annealing temperature on the operation panel 23, and select a suitable heating rate. Enable the water bath temperature adjustment structure for preheating.

[0030] The stirring speed and time are set according to the properties of the reaction system, and the stirring motor 11 is used to ensure uniform mixing of the reaction solution.

[0031] Add the prepared nucleic acid sample, primers and other reagents to the test tube in sequence according to the experimental plan. Avoid the generation of bubbles to ensure the uniformity of the reaction.

[0032] Close the tank cover 7 and ensure that its automatic lock 8 structure is locked to prevent leakage or contamination during the reaction.

[0033] Start the reaction program and allow the device to cycle and amplify. Monitor the progress of the reaction and ensure that the temperature control and stirring functions are functioning properly.

[0034] When the thermal cycle is completed, release the tank cover 7 and take out the culture tube 6. Be careful to avoid touching hot parts during operation.

[0035] Perform subsequent analysis steps, such as using electrophoresis or real-time PCR equipment to detect amplification results.

[0036] After the reaction is completed, clean the reaction tank and related components in time to prevent cross contamination. Appropriate cleaning agents and disinfectants can be used.

[0037] Regularly inspect and maintain the equipment to ensure it is in good working condition for a long time.

[0038] It should be noted that, in this document, relational terms such as first and second, etc. are merely used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations.

[0039] The above description is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A reaction tank for nucleic acid preparation and amplification, comprising a main frame (1), characterized in that: A storage box (2) is fixedly mounted on the upper wall of the main frame (1); an isolation frame (3) is fixedly mounted inside the storage box (2); a main tank body (4) is fixedly mounted inside the isolation frame (3); a test tube rack (5) is fixedly mounted inside the main tank body (4); a culture tube (6) is fixedly mounted inside the test tube rack (5); an auxiliary stirring structure is mounted inside the main tank body (4); and a water bath temperature control structure is mounted inside the main tank body (4).

2. A reaction tank for nucleic acid preparation and amplification according to claim 1, characterized in that A tank cover (7) is rotatably mounted on the upper wall of the main tank body (4), an automatic lock (8) is fixedly mounted on the other end of the tank cover (7), an electric push rod (9) is rotatably mounted on the upper end of the storage box (2), and the telescopic end of the electric push rod (9) is rotatably mounted on the side wall of the tank cover (7).

3. A reaction tank for nucleic acid preparation and amplification according to claim 2, characterized in that The auxiliary stirring structure comprises a stirring frame (10), the stirring frame (10) is fixedly mounted on the upper end of the main tank body (4) and is located on the inner side of the tank body cover (7), a stirring motor (11) is fixedly mounted inside the stirring frame (10), a stirring shaft (12) is fixedly mounted on the driving end of the stirring motor (11), and a stirring head (13) is fixedly mounted on the stirring shaft (12).

4. A reaction tank for nucleic acid preparation and amplification according to claim 1, characterized in that The water bath temperature control structure comprises a water storage tank (14), a water bath chamber (15) is provided in the main tank body (4), the water storage tank (14) is fixedly installed in the reserve tank (2), a water pump (16) is fixedly installed in the water storage tank (14), the output end of the water pump (16) is fixedly connected to the main tank body (4), a drain pipe (17) is fixedly installed on the water bath chamber (15), and the drain pipe (17) is fixedly connected to the water storage tank (14).

5. A reaction tank for nucleic acid preparation and amplification according to claim 4, characterized in that An auxiliary heating tube (18) is fixedly installed in the water bath chamber (15), and the auxiliary heating tube (18) is a resistance electric heating spiral tube.

6. A reaction tank for nucleic acid preparation and amplification according to claim 4, characterized in that A semiconductor refrigeration sheet (19) is fixedly mounted on the rear wall of the water storage tank (14); a cold end of the semiconductor refrigeration sheet (19) is fixedly mounted on the side wall of the water storage tank (14); a heat sink (20) is fixedly mounted on the hot end of the semiconductor refrigeration sheet (19); and an air supply fan (21) is fixedly mounted inside the heat sink (20).

7. A reaction tank for nucleic acid preparation and amplification according to claim 1, characterized in that A storage battery (22) is fixedly installed in the storage box (2), an operation panel (23) is fixedly installed at the front end of the storage box (2), and a support column (24) is fixedly installed at the lower end of the main frame (1).

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