Magnetic rod device for plasmid purification

Abstract

The utility model provides a magnetic bar device for plasmid purification, which comprises a mounting frame, a magnetic sleeve lifting component, a push sleeve lifting component and a magnetic sleeve, a connecting sleeve is arranged on the magnetic sleeve lifting component, and a clamping mechanism for clamping the magnetic sleeve is arranged on the connecting sleeve; a push sleeve corresponding to the connecting sleeve is arranged on the push sleeve lifting part, and the push sleeve can extend into the connecting sleeve; when the magnetic sleeve is detached, the push sleeve abuts against the upper end of the magnetic sleeve and moves downwards so that the magnetic sleeve can be separated from the connecting sleeve. According to the device, the magnetic sleeve is automatically detached, manual operation and intervention are not needed, the trouble of manual operation is saved, and the automation level of enterobacter plasmid DNA extraction operation can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experimental equipment, in particular to a magnetic rod device used for plasmid purification. Background Art

[0002] At present, the extraction and purification of Escherichia coli plasmid DNA requires steps such as injection, oscillation, magnetic bead extraction, washing, and elution. The pure plasmid DNA after extraction can be directly used for applications such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection.

[0003] Among them, when extracting plasmid DNA, magnetic bead extraction is used. The magnetic bead extraction method is a method that uses the functional groups on the surface of magnetic particles to specifically interact with the target substance, so that the target substance is fixed on the surface of the magnetic particles, and then the magnetic field is used to separate and concentrate the target substance1. When using the magnetic bead extraction method for extraction, the magnetic rod device is a key component.

[0004] The core components of the magnetic rod device are the magnetic sleeve and the magnetic rod, wherein the magnetic sleeve and the magnetic rod are respectively arranged on the lifting drive mechanism, and the lifting drive mechanism can drive the magnetic sleeve and the magnetic rod to rise and fall independently, and the magnetic rod can be inserted into the magnetic sleeve, and the magnetic rod corresponds to the magnetic sleeve one by one. When the magnetic rod moves downward relative to the magnetic sleeve, the magnetic rod extends into the magnetic sleeve, so that the outer surface of the magnetic sleeve has magnetism and can adsorb the magnetic beads in the bacterial solution, and the plasmids are fixed on the magnetic beads, thereby extracting the plasmids; when the magnetic rod moves upward relative to the magnetic sleeve, the magnetic rod can be pulled out of the magnetic sleeve, and the magnetic field on the outer surface of the magnetic sleeve disappears, so that the magnetic beads attached to the outer surface of the magnetic sleeve fall off, and the magnetic beads are released.

[0005] After the magnetic rod device is used once, the magnetic sleeve needs to be removed and replaced. In the traditional magnetic rod device, the removal of the magnetic sleeve requires manual operation and intervention, which is troublesome to operate. In addition, the manual removal of the magnetic sleeve reduces the automation level of the entire operation process, which is not conducive to realizing the demand for automated operation. Summary of the invention

[0006] The utility model aims to solve the problem that the existing magnetic rod device needs to manually disassemble the magnetic sleeve during use, the operation is troublesome, and the operation automation is low. A magnetic rod device for plasmid purification is provided, which can effectively solve the above problems.

[0007] The utility model aims to realize a magnetic rod device for plasmid purification through the following technical scheme, comprising a mounting frame, a magnetic sleeve lifting component, a push sleeve lifting component, and a magnetic sleeve, wherein a connecting sleeve is arranged on the magnetic sleeve lifting component, and a clamping mechanism for clamping the magnetic sleeve is arranged on the connecting sleeve; a push sleeve corresponding to the connecting sleeve is arranged on the push sleeve lifting component, and the push sleeve can be extended into the connecting sleeve; when the magnetic sleeve is disassembled, the push sleeve presses against the upper end of the magnetic sleeve and moves downward to separate the magnetic sleeve from the connecting sleeve.

[0008] Preferably, the clamping mechanism includes a steel ball hole arranged in the circumferential direction of the connecting sleeve, a steel ball is connected to the steel ball hole, an elastic ring groove is provided on the periphery of the steel ball hole, an elastic ring is connected to the elastic ring groove, and the elastic ring is located on the periphery of the steel ball; an annular groove matching the steel ball is provided on the outer side of one end of the magnetic sleeve; when the magnetic sleeve is connected to the connecting sleeve, the steel ball is embedded in the annular groove on the magnetic sleeve.

[0009] Preferably, the elastic ring is made of rubber.

[0010] Preferably, the mounting frame is provided with a guide rail, and the magnetic sleeve lifting component includes a first sliding member slidably connected to the guide rail, the first sliding member is provided with a magnetic sleeve mounting plate, and the connecting sleeve is arranged at the lower end of the magnetic sleeve mounting plate; the mounting frame is provided with a magnetic sleeve lifting drive mechanism for driving the first sliding member to move up and down.

[0011] Preferably, the magnetic sleeve lifting drive mechanism comprises a magnetic sleeve lifting motor arranged on a mounting frame, the magnetic sleeve lifting motor is connected to a magnetic sleeve lifting screw rod, and the magnetic sleeve lifting screw rod is threadedly matched with the first sliding member.

[0012] Preferably, it also includes a magnetic rod lifting component, to which a magnetic rod corresponding to the magnetic sleeve is connected, and the magnetic rod passes through the push sleeve.

[0013] Preferably, the magnetic rod lifting component includes a second sliding member slidably connected to the guide rail, the second sliding member is provided with a magnetic rod mounting plate, and the magnetic rod is fixedly mounted on the lower end of the magnetic rod mounting plate; the mounting frame is provided with a magnetic rod lifting drive mechanism for driving the magnetic rod mounting plate to move up and down.

[0014] Preferably, the magnetic rod lifting drive mechanism comprises a magnetic rod lifting motor arranged on a mounting frame, a magnetic rod lifting screw is connected to the magnetic rod lifting motor, and the magnetic rod lifting screw is threadably matched with the second sliding member.

[0015] Preferably, the push sleeve lifting component includes a push sleeve mounting plate, a guide rod arranged on the upper end of the magnetic rod mounting plate, a fixed plate is arranged on the top end of the guide rod, a lifting plate is slidably connected to the guide rod, a push sleeve lifting motor is arranged on the fixed plate, a push sleeve lifting motor is connected to a push sleeve lifting screw, and the push sleeve lifting screw is threadedly matched with the lifting plate; a connecting rod is arranged on the lifting plate, the push sleeve mounting plate is connected to the connecting rod, the push sleeve mounting plate is located between the magnetic rod mounting plate and the magnetic sleeve mounting plate, and the push sleeve is arranged on the push sleeve mounting plate.

[0016] The beneficial effects of the utility model are as follows: the utility model realizes the automatic disassembly of the magnetic sleeve without manual operation and intervention, thus eliminating the trouble of manual operation and facilitating the improvement of the automation level of the operation of extracting the enterobacterial plasmid DNA. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a structural schematic diagram of one direction of the utility model.

[0018] Figure 2 It is a structural schematic diagram of another direction of the utility model.

[0019] Figure 3 It is a structural schematic diagram of the magnetic sleeve.

[0020] Figure 4 It is a schematic diagram of the structure when the magnetic sleeve, magnetic rod and push sleeve are coordinated.

[0021] In the figure: 1, mounting frame, 2, guide rail, 3, magnetic sleeve mounting plate, 4, first sliding member, 5, connecting sleeve, 6, magnetic sleeve, 7, magnetic sleeve lifting screw, 8, magnetic sleeve lifting motor, 9, first pulley, 10, second pulley, 11, transmission belt, 12, push sleeve mounting plate, 13, push sleeve, 14, guide rod, 15, lifting plate, 16, fixed plate, 17, push sleeve lifting motor, 18, push sleeve lifting screw, 19, connecting rod, 20, guide rail, 21, magnetic rod mounting plate, 22, magnetic rod, 23, second sliding member, 24, magnetic rod lifting screw, 25, magnetic rod lifting motor, 28, annular groove, 29, steel ball, 30, elastic ring groove, 31, elastic ring. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.

[0023] like Figures 1 to 4 As shown, a magnetic rod device for plasmid purification includes a mounting frame 1, a magnetic sleeve lifting component, a push sleeve lifting component, a magnetic rod lifting component, and a magnetic sleeve 6. The magnetic sleeve 6 is a tubular structure with a closed lower end and an open upper end. A connecting sleeve 5 is provided on the magnetic sleeve lifting component. A clamping mechanism for clamping the magnetic sleeve 6 is provided on the connecting sleeve 5. The magnetic sleeve lifting component drives the magnetic sleeve 6 to move up and down. A push sleeve 13 corresponding to the connecting sleeve 5 is provided on the push sleeve lifting component. The push sleeve 13 is annular and is driven to move up and down by the push sleeve lifting component. The push sleeve 13 can be extended into the connecting sleeve 5. When the magnetic sleeve 6 is disassembled, the push sleeve 13 presses against the upper end of the magnetic sleeve 6 and moves downward to separate the magnetic sleeve 6 from the connecting sleeve 5.

[0024] Among them, the clamping mechanism includes a steel ball 29 hole arranged in the circumferential direction of the connecting sleeve 5, a steel ball 29 is connected to the steel ball 29 hole, an elastic ring groove 30 is arranged on the periphery of the steel ball 29 hole, an elastic ring 31 is connected to the elastic ring groove 30, the elastic ring 31 is located on the periphery of the steel ball 29, and the steel ball 29 contacts the elastic ring 31. An annular groove 28 matching with the steel ball 29 is arranged on the outer side of one end of the magnetic sleeve 6. When the magnetic sleeve 6 is connected to the connecting sleeve 5, the steel ball 29 is embedded in the annular groove 28 on the magnetic sleeve 6. The steel ball 29 can have a certain activity space in the steel ball 29 groove.

[0025] The elastic ring 31 is made of elastic material and can apply a contraction force along the radial direction of the elastic ring 31 to the steel ball 29. The upper end of the magnetic sleeve 6 is inserted into the connecting sleeve 5 and connected to the connecting sleeve 5. When the magnetic sleeve 6 is connected to the connecting sleeve 5, the steel ball 29 is embedded in the annular groove 28 at the upper end of the magnetic sleeve 6. The steel ball 29 presses against the inner wall of the annular groove 28 under the action of the elastic ring 31, thereby fixing the magnetic sleeve 6. When the magnetic sleeve 6 is disassembled, the push sleeve is driven downward by the push sleeve lifting component, and the push sleeve extends into the connecting sleeve 5 and presses against the upper end of the magnetic sleeve 6. As the push sleeve continues to move downward, the magnetic sleeve 6 overcomes the pressing force of the steel ball 29 and detaches from the connecting sleeve 5, and the magnetic sleeve 6 is automatically disassembled. In this embodiment, the elastic ring 31 is made of rubber.

[0026] Specifically, the mounting frame 1 is provided with a guide rail 2, which is arranged along the vertical direction. The magnetic sleeve lifting component includes a first sliding member 4 slidably connected to the guide rail 2, and a magnetic sleeve mounting plate 3 is provided on the first sliding member 4, and a connecting sleeve 5 is arranged at the lower end of the magnetic sleeve mounting plate 3. The mounting frame 1 is provided with a magnetic sleeve lifting driving mechanism for driving the first sliding member 4 to move up and down. The magnetic sleeve lifting driving mechanism includes a magnetic sleeve lifting motor 8 arranged on the mounting frame 1, and the magnetic sleeve lifting motor 8 is connected to a magnetic sleeve lifting screw 7, which is arranged parallel to the guide rail 2, and the magnetic sleeve lifting screw 7 is threadedly matched with the first sliding member 4. Among them, the magnetic sleeve lifting motor 8 is connected to a first pulley 9, and the upper end of the magnetic sleeve lifting screw 7 is connected to a second pulley 10, and the first pulley 9 and the second pulley 10 are connected by a transmission belt 11. The magnetic sleeve lifting motor 8 drives the magnetic sleeve lifting screw 7 to rotate, and the magnetic sleeve lifting screw 7 drives the first sliding member 4 to move up and down, thereby driving the magnetic sleeve mounting plate 3 and the magnetic sleeve 6 to move up and down. The lifting of the magnetic sleeve 6 is realized.

[0027] The magnetic rod lifting component is connected with a magnetic rod 22 corresponding to the magnetic sleeve 6, and the magnetic rod 22 passes through the push sleeve. The magnetic rod 22 is driven up and down by the magnetic rod lifting component, and the magnetic rod 22 and the magnetic sleeve 6 are coaxially arranged.

[0028] Specifically, the magnetic rod lifting component includes a second sliding member 23 slidably connected to the guide rail 2, a magnetic rod mounting plate 21 is provided on the second sliding member 23, and a magnetic rod 22 is fixedly installed at the lower end of the magnetic rod mounting plate 21; a magnetic rod lifting driving mechanism for driving the magnetic rod mounting plate 21 to move up and down is provided on the mounting frame 1. The magnetic rod lifting driving mechanism includes a magnetic rod lifting motor 25 arranged on the mounting frame 1, a magnetic rod lifting screw 24 is connected to the magnetic rod lifting motor 25, the magnetic rod lifting screw 24 is parallel to the guide rail 2, and the magnetic rod lifting screw 24 is threadedly matched with the second sliding member 23. The magnetic rod lifting screw 24 is driven to rotate by the magnetic rod lifting motor 25, driving the second sliding member 23 to move up and down along the guide rail 2, thereby driving the magnetic rod 22 to move up and down, thereby realizing the lifting of the magnetic rod 22.

[0029] The push sleeve lifting component includes a push sleeve mounting plate 12, a guide rod 14 arranged at the upper end of a magnetic rod mounting plate 21, the guide rod 14 is arranged in a vertical direction, a fixed plate 16 is arranged at the top of the guide rod 14, a lifting plate 15 is slidably connected to the guide rod 14, a push sleeve lifting motor 17 is arranged on the fixed plate 16, a push sleeve lifting screw 18 is connected to the push sleeve lifting motor 17, and the push sleeve lifting screw 18 is threadedly matched with the lifting plate 15. A connecting rod 19 is arranged on the lifting plate 15, and the push sleeve mounting plate 12 is connected to the connecting rod 19. The push sleeve mounting plate 12 is located between the magnetic rod mounting plate 21 and the magnetic sleeve mounting plate 3, and the push sleeve is arranged on the push sleeve mounting plate 12. The push sleeve lifting screw 18 is driven to rotate by the push sleeve lifting motor 17, so as to drive the lifting plate 15 to move up and down, thereby driving the push sleeve to move up and down, so as to realize the lifting of the push sleeve.

[0030] The utility model realizes the automatic disassembly of the magnetic sleeve without manual operation and intervention, saves the trouble of manual operation, and is beneficial to improving the automation level of the operation of extracting enterobacterial plasmid DNA.

[0031] The present utility model is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present utility model. However, no matter what changes are made in the shape or structure, all those having the same or similar technical solutions as the present application fall within the protection scope of the present utility model.

Claims

1. A magnetic rod device for plasmid purification, characterized in that: It includes a mounting frame, a magnetic sleeve lifting component, a push sleeve lifting component, and a magnetic sleeve. A connecting sleeve is provided on the magnetic sleeve lifting component, and a clamping mechanism for clamping the magnetic sleeve is provided on the connecting sleeve. A push sleeve corresponding to the connecting sleeve is provided on the push sleeve lifting component, and the push sleeve can be extended into the connecting sleeve. When the magnetic sleeve is disassembled, the push sleeve presses against the upper end of the magnetic sleeve and moves downward to separate the magnetic sleeve from the connecting sleeve.

2. A magnetic rod device for plasmid purification according to claim 1, characterized in that: The clamping mechanism includes a steel ball hole arranged in the circumferential direction of the connecting sleeve, a steel ball is connected in the steel ball hole, an elastic ring groove is arranged on the periphery of the steel ball hole, an elastic ring is connected in the elastic ring groove, and the elastic ring is located on the periphery of the steel ball; an annular groove matching with the steel ball is arranged on the outer side of one end of the magnetic sleeve; when the magnetic sleeve is connected to the connecting sleeve, the steel ball is embedded in the annular groove on the magnetic sleeve.

3. A magnetic rod device for plasmid purification according to claim 2, characterized in that: The elastic ring is made of rubber.

4. A magnetic rod device for plasmid purification according to any one of claims 1 to 3, characterized in that: The mounting frame is provided with a guide rail, and the magnetic sleeve lifting component includes a first sliding member slidably connected to the guide rail, the first sliding member is provided with a magnetic sleeve mounting plate, and the connecting sleeve is arranged at the lower end of the magnetic sleeve mounting plate; the mounting frame is provided with a magnetic sleeve lifting drive mechanism for driving the first sliding member to move up and down.

5. A magnetic rod device for plasmid purification according to claim 4, characterized in that: The magnetic sleeve lifting drive mechanism comprises a magnetic sleeve lifting motor arranged on a mounting frame, the magnetic sleeve lifting motor is connected to a magnetic sleeve lifting screw rod, and the magnetic sleeve lifting screw rod is threadedly matched with the first sliding member.

6. A magnetic rod device for plasmid purification according to claim 4, characterized in that: It also includes a magnetic rod lifting component, which is connected with a magnetic rod corresponding to the magnetic sleeve, and the magnetic rod passes through the push sleeve.

7. A magnetic rod device for plasmid purification according to claim 6, characterized in that: The magnetic rod lifting component includes a second sliding member slidably connected to the guide rail, the second sliding member is provided with a magnetic rod mounting plate, and the magnetic rod is fixedly installed at the lower end of the magnetic rod mounting plate; the mounting frame is provided with a magnetic rod lifting drive mechanism for driving the magnetic rod mounting plate to move up and down.

8. A magnetic rod device for plasmid purification according to claim 7, characterized in that: The magnetic rod lifting drive mechanism comprises a magnetic rod lifting motor arranged on a mounting frame, a magnetic rod lifting screw rod is connected to the magnetic rod lifting motor, and the magnetic rod lifting screw rod is threadedly matched with the second sliding member.

9. A magnetic rod device for plasmid purification according to claim 7, characterized in that: The push sleeve lifting component includes a push sleeve mounting plate, a guide rod arranged on the upper end of the magnetic rod mounting plate, a fixed plate is arranged on the top end of the guide rod, a lifting plate is slidably connected to the guide rod, a push sleeve lifting motor is arranged on the fixed plate, a push sleeve lifting screw is connected to the push sleeve lifting motor, and the push sleeve lifting screw is threadedly matched with the lifting plate; a connecting rod is arranged on the lifting plate, the push sleeve mounting plate is connected to the connecting rod, the push sleeve mounting plate is located between the magnetic rod mounting plate and the magnetic sleeve mounting plate, and the push sleeve is arranged on the push sleeve mounting plate.

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