Full-automatic plasmid purification instrument

Abstract

The utility model provides a full-automatic plasmid purification instrument which comprises a base, an oscillation device, a reagent strip device, a first moving device and a liquid injection device are arranged on the base, a washing area and an elution area are arranged on the reagent strip device, and the liquid injection device is used for injecting reagents into hole sites in the oscillation device; the first moving device is provided with a magnetic bar magnetic sleeve part, the magnetic bar magnetic sleeve part comprises a moving frame connected with the first moving device, the moving frame is provided with a liftable magnetic sleeve and a magnetic bar, and the magnetic bar can extend into the magnetic sleeve; the movable frame is further provided with a cutting device. When the escherichia coli plasmid DNA extraction and purification device is used for extracting and purifying escherichia coli plasmid DNA, the steps of liquid injection, oscillation, magnetic bead extraction, cleaning, elution and the like can be automatically completed, manual intervention is not needed in the whole extraction and purification process, the labor cost is saved, and the working efficiency is high; and the whole extraction and purification process does not need manual operation intervention, so that the risk that the reagent is polluted due to improper manual operation is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experimental equipment, in particular to a full-automatic plasmid purification instrument. Background Art

[0002] At present, the extraction and purification of Escherichia coli plasmid DNA requires steps such as injection, oscillation, magnetic bead extraction, washing, and elution. The pure plasmid DNA after extraction can be directly used for applications such as enzyme digestion, PCR, sequencing, ligation, transformation, and transfection.

[0003] At present, the entire process of extracting and purifying E. coli plasmid DNA requires manual operation and intervention, which is cumbersome to operate and has low work efficiency. In addition, when extracting and purifying E. coli plasmid DNA manually, improper operation can easily cause sample contamination. Summary of the invention

[0004] The utility model aims to solve the deficiencies in the prior art and provide a fully automatic plasmid purification instrument.

[0005] The purpose of the utility model is achieved through the following technical scheme: a fully automatic plasmid purification instrument, including a base, on which an oscillation device, a reagent strip device, a first moving device, and a liquid injection device are provided, the reagent strip device is provided with a washing area and an elution area, the liquid injection device is used to inject reagents into the holes on the oscillation device; the first moving device is provided with a magnetic rod and a magnetic sleeve component, the magnetic rod and the magnetic sleeve component includes a moving frame connected to the first moving device, the moving frame is provided with a liftable magnetic sleeve and a magnetic rod, the magnetic rod can extend into the magnetic sleeve; the moving frame is also provided with a breaking device.

[0006] Preferably, the movable frame is provided with a guide rail, on which a first lifting seat and a second lifting seat are slidably connected, the first lifting seat is connected with a first lifting plate, and a magnetic rod is installed at the lower end of the first lifting plate; the second lifting seat is connected with a second lifting plate, the second lifting plate is located below the first lifting plate, and the magnetic sleeve is installed at the lower end of the second lifting plate and corresponds to the magnetic rod one by one; the movable frame is provided with a first lifting drive device for driving the first lifting plate to move up and down, and a second lifting drive device for driving the second lifting plate to move up and down.

[0007] Preferably, the first lifting drive device comprises a magnetic rod lifting motor arranged on the movable frame, a magnetic rod lifting screw connected to the magnetic rod lifting motor, and the magnetic rod lifting screw is threadedly matched with the first lifting seat.

[0008] Preferably, the second lifting drive device comprises a magnetic sleeve lifting motor arranged on the movable frame, a magnetic sleeve lifting screw connected to the magnetic sleeve lifting motor, and the magnetic sleeve lifting screw is threadedly matched with the second lifting seat.

[0009] Preferably, the breaking device includes a guide frame, a third lifting plate, and a cutter head mounting plate. The guide frame is fixedly arranged on the movable frame, a guide rod is provided on the lifting plate, the guide rod and the guide frame are slidably matched, the cutter head mounting plate is connected to the lower end of the guide rod, a cutter head is provided at the lower end of the cutter head mounting plate, and a third lifting drive device for driving the third lifting plate to move up and down is provided on the guide frame.

[0010] Preferably, the third lifting drive device includes a tool head lifting motor arranged on the guide frame, the tool head lifting motor is connected to a tool head lifting screw, a screw nut is provided on the third lifting plate, and the tool head lifting screw is threadedly matched with the screw nut.

[0011] Preferably, the liquid injection device comprises a second moving device, a liquid injection substrate is provided on the second moving device, and a liquid injection head is provided on the liquid injection substrate.

[0012] Preferably, a liquid collecting tank is provided at one end of the second moving device.

[0013] Preferably, the magnetic rod magnetic sleeve component also includes an anti-drip device, which includes an anti-drip tray and a tray motor. The anti-drip tray is slidably connected to the mobile frame, and the tray motor is arranged on the mobile frame. A screw rod is connected to the tray motor, and the screw rod is threadedly matched with the anti-drip tray.

[0014] The beneficial effects of the utility model are as follows: when extracting and purifying Escherichia coli plasmid DNA, the utility model can automatically complete the steps of liquid injection, oscillation, magnetic bead extraction, cleaning, elution and the like, and the entire extraction and purification process does not require human intervention, thus saving labor costs and improving work efficiency; and since the entire extraction and purification process does not require human intervention, the risk of reagent contamination due to improper human operation is reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural schematic diagram of one direction of the utility model.

[0016] Figure 2 It is a structural schematic diagram of another direction of the utility model.

[0017] Figure 3 This is a schematic diagram of the structure of the magnetic rod and magnetic sleeve components in one direction.

[0018] Figure 4 It is a schematic diagram of the structure of the magnetic rod and magnetic sleeve components in another direction.

[0019] Figure 5 It is a schematic diagram of the structure of the anti-drip device.

[0020] Figure 6 This is a schematic diagram of the structure of the utility model after the magnetic rod and magnetic sleeve components are removed.

[0021] In the figure: 1, base, 2, reagent strip device, 21, washing area, 22, elution area, 3, shaking device, 4, first moving device, 5, magnetic rod and magnetic sleeve component, 51, moving frame, 52, guide rail, 53, first lifting plate, 54, magnetic rod, 55, first lifting seat, 56, magnetic rod lifting screw, 57, magnetic rod lifting motor, 58, second lifting plate, 59, magnetic sleeve, 510, second lifting seat, 511, magnetic sleeve lifting screw, 512, transmission belt, 513, first pulley, 514, second Pulley, 515, magnetic sleeve lifting motor, 516, cutter head, 517, cutter head mounting plate, 518, guide frame, 519, cutter head lifting motor, 520, guide rod, 521, guide sleeve, 522, third lifting plate, 523, screw nut, 524, cutter head lifting screw, 525, anti-drip tray, 526, tray motor, 527, guide rod, 528, guide block, 7, liquid injection device, 71, second moving device, 72, liquid injection substrate, 73, liquid injection head, 74, liquid collecting tank, 8, reagent strip. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.

[0023] like Figures 1 to 6 As shown, a fully automatic plasmid purification instrument includes a base 1, on which an oscillating device 3, a reagent strip device 2, a first moving device 4, and a liquid injection device 7 are provided. The reagent strip device is provided with a washing area 21 and an elution area 22. The oscillating device 3, the washing area 21, and the elution area 22 are all provided with holes, and the holes on the elution area 3 and the oscillating device 3 are used to place reagent tubes. The liquid injection device 7 is used to inject reagents into the holes on the oscillating device 3. A magnetic rod magnetic sleeve component 5 is provided on the first moving device 4, and the magnetic rod magnetic sleeve component 5 is driven by the first moving device 4 to move linearly in the horizontal direction. The magnetic rod magnetic sleeve component 5 includes a moving frame 51 connected to the first moving device 4, and a liftable magnetic sleeve 59 and a magnetic rod 54 are provided on the moving frame 51, and the magnetic rod 54 can extend into the magnetic sleeve 59.

[0024] In this embodiment, the first moving device 4 is an electric guide rail.

[0025] The movable frame 51 is provided with a guide rail 52, which is arranged in the vertical direction. The first lifting seat 55 and the second lifting seat 510 are slidably connected to the guide rail 52. The first lifting seat 55 is connected with the first lifting plate 53, and the magnetic rod 54 is installed at the lower end of the first lifting plate 53. The second lifting seat 510 is connected with the second lifting plate 58, which is located below the first lifting plate 53. The magnetic sleeve 59 is installed at the lower end of the second lifting plate 58 and corresponds to the magnetic rod 54 one by one. The movable frame 51 is provided with a first lifting drive device for driving the first lifting plate 53 to move up and down and a second lifting drive device for driving the second lifting plate 58 to move up and down. The magnetic rod 54 is driven to move up and down by the first lifting drive device, and the magnetic sleeve 59 is driven to move up and down by the second lifting drive device. When the magnetic rod 54 moves upward relative to the magnetic sleeve 59, the magnetic rod 54 is pulled out of the magnetic sleeve 59; when the magnetic rod 54 moves downward relative to the magnetic sleeve 59, the magnetic rod 54 extends into the magnetic sleeve 59. The magnetic sleeve 59 is a tubular structure with a closed lower end and an open upper end, and the magnetic rod 54 can extend into the magnetic sleeve from the opening at the upper end.

[0026] The first lifting drive device includes a magnetic rod lifting motor 57 disposed on the moving frame 51, a magnetic rod lifting screw 56 connected to the magnetic rod lifting motor 57, the magnetic rod lifting screw 56 is parallel to the guide rail 52, and the magnetic rod lifting screw 56 is threadedly matched with the first lifting seat 55. The magnetic rod lifting motor 57 drives the magnetic rod lifting screw 56 to rotate, and the rotation of the magnetic rod lifting screw 56 drives the first lifting seat 55 to move up and down along the guide rail 52.

[0027] The second lifting drive device includes a magnetic sleeve lifting motor 515 arranged on the moving frame 51, a magnetic sleeve lifting screw 511 connected to the magnetic sleeve lifting motor 515, the magnetic sleeve lifting screw 511 is parallel to the guide rail 52, and the magnetic sleeve lifting screw 511 is threadedly matched with the second lifting seat 510. Among them, a first pulley 513 is provided on the upper end of the magnetic sleeve lifting screw 511, and a second pulley 514 is provided on the output shaft of the magnetic sleeve lifting motor 515. The first pulley 513 and the second pulley 514 are connected by a transmission belt 512. The magnetic sleeve lifting screw 511 is driven to rotate by the magnetic sleeve lifting motor 515, and the magnetic sleeve lifting screw 511 drives the second lifting seat 510 to move up and down along the guide rail 52 when rotating.

[0028] The mobile frame 51 is also provided with a device for breaking. The breaking device includes a guide frame 518, a third lifting plate 522, and a cutter head mounting plate 517. The guide frame 518 is fixedly arranged on the mobile frame 51. The lifting plate is provided with a guide rod 520, which is arranged along the vertical direction. The guide rod 520 and the guide frame 518 are slidably matched. The guide frame 518 is provided with a guide sleeve 521, and the guide rod 520 passes through the guide sleeve 521. The cutter head mounting plate 517 is connected to the lower end of the guide rod 520. The cutter head 516 is provided at the lower end of the cutter head mounting plate 517. The cutter head 516 is made of cemented carbide, and the lower end of the cutter head 516 is sharp. The guide frame 518 is provided with a third lifting drive device for driving the third lifting plate 522 to move up and down.

[0029] The third lifting drive device includes a cutter head lifting motor 519 arranged on the guide frame 518, a cutter head lifting screw 524 is connected to the cutter head lifting motor 519, and a screw nut 523 is provided on the third lifting plate 522, and the cutter head lifting screw 524 is threadedly matched with the screw nut 523. The cutter head lifting screw 524 is driven to rotate by the cutter head lifting motor 519, thereby driving the cutter head 516 to move up and down. In some cases, the reagent tubes placed in the holes on the elution area 22 and the washing area 21 will be provided with sealing films, and the breaking device is used to open the sealing films; when opening the sealing films, the cutter head 516 moves to the top of the sealing films of the reagent tubes, and the cutter head 516 is driven to move downward by the third lifting drive device so that the cutter head 516 breaks the sealing films, thereby opening the sealing films, and making an opening on the sealing films, so as to facilitate the subsequent magnetic sleeve 59 and the magnetic rod 54 to extend into the holes.

[0030] The liquid injection device 7 includes a second moving device 71, on which a liquid injection substrate 72 is provided. The liquid injection substrate 72 is driven to move linearly by the second moving device 71. A liquid injection head 73 is provided on the liquid injection substrate 72, and the liquid injection head 73 is connected to the reagent supply device through a connecting pipe. Among them, multiple liquid injection heads 73 can be arranged on the liquid injection substrate 72 at the same time, and each liquid injection head 73 can inject a specific reagent into the hole on the oscillation device 3. The reagent can be Buffer A1 reagent, Buffer B1 reagent, Buffer C1 reagent, Buffer D1. In this embodiment, the second moving device 71 is an electric guide rail.

[0031] Furthermore, a liquid collecting tank 74 is provided at one end of the second moving device 71. After the liquid injection head 73 injects the reagent into the hole on the oscillating device 3, the second moving device 71 drives the liquid injection head 73 to move above the liquid collecting tank 74, and cleans the liquid injection head and the pipeline with clean water. The clean water after flushing the internal pipeline is discharged from the liquid injection head and injected into the liquid collecting tank 74.

[0032] The magnetic rod and magnetic sleeve component also includes an anti-drip device. The anti-drip device includes an anti-drip tray 525 and a tray motor 526, and the anti-drip tray 525 is slidably connected to the mobile frame 51. Among them, the anti-drip tray 525 is provided with a guide rod 527, and the mobile frame 51 is provided with a guide block 528 corresponding to the guide rod 527, and the guide rod 527 and the guide block 528 are slidably matched. The tray motor 526 is arranged on the mobile frame, and the tray motor 526 is connected with a screw rod, and the screw rod is threadedly matched with the anti-drip tray 525. The anti-drip tray 525 is driven to move horizontally by the tray motor 526. When the operation of the magnetic sleeve 59 and the magnetic rod 54 is completed, the anti-drip tray 6 moves to the bottom of the magnetic sleeve 59 and the magnetic rod 54, and the anti-drip tray 6 catches the liquid dripping from the surface of the magnetic sleeve 59, thereby preventing the liquid from dripping to the area outside the anti-drip tray 6 and causing pollution to the inside of the equipment.

[0033] The utility model comprises the following specific steps when extracting and purifying Escherichia coli plasmid DNA:

[0034] Step 1: first, insert the reagent tube into the hole position on the oscillating device 3, inject the cultured Escherichia coli liquid into all the holes of the oscillating device, inject the magnetic beads and the washing liquid mixture into two or more rows of holes of the reagent strip, and install the reagent strip 8 into the reagent strip device 2. The reagent strip 8 is pre-loaded with washing liquid and elution liquid. The washing liquid is located in the washing area 21 on the reagent strip device 2, and the elution liquid is located in the elution area 22 on the reagent strip device 2;

[0035] Step 2: Inject Buffer A1, Buffer B1, Buffer C1 and Buffer D1 into the Escherichia coli liquid wells on the oscillating device through the injection device 7.

[0036] Step 3: The magnetic rod and magnetic sleeve component 5 moves to above the E. coli liquid hole position of the oscillating device, and the magnetic sleeve 59 descends alone into the E. coli liquid hole position. The second lifting drive device drives the magnetic sleeve 59 to move up and down at high frequency. The up and down high-frequency movement of the magnetic sleeve 59 performs up and down high-frequency oscillation on the liquid in the E. coli liquid hole position, thereby achieving neutralization, mixing and resuspension of the E. coli liquid and the reagent.

[0037] Step 4. After the shock treatment, the plasmids in the Escherichia coli liquid have been separated from other impurities. Then the magnetic rod and magnetic sleeve component 5 are moved to the top of the hole pre-loaded with magnetic beads and washing solution. The magnetic sleeve 59 is first lowered to the hole pre-loaded with magnetic beads and washing solution by the second lifting drive device. The magnetic sleeve 59 oscillates up and down at a high frequency to achieve mixing of the magnetic beads. Then the first lifting drive device drives the magnetic rod 54 to descend and penetrate into the magnetic sleeve 59, and the magnetic sleeve 59 and the magnetic rod 54 are extended into the bottom of the hole together, pause for a certain period of time, and the magnetic beads are adsorbed by the magnetic rod 54. Then the magnetic rod 54 and the magnetic sleeve 59 rise slowly and synchronously.

[0038] Step 5. The magnetic rod and magnetic sleeve component 5 moves to the top of the hole of the E. coli liquid in the oscillating device, and the magnetic rod 54 and the magnetic sleeve 59 are synchronously and slowly descended to the bottom of the liquid hole, and then the magnetic rod 54 rises to make the magnetic beads attached to the outer surface of the magnetic sleeve 59 fall off, and the liquid in the E. coli liquid hole is oscillated and mixed up and down by the high-frequency up and down movement of the magnetic sleeve 59; then the magnetic rod 54 descends and penetrates into the magnetic sleeve 59 and extends to the bottom of the hole together with the magnetic rod, and the magnetic beads are adsorbed on the outer surface of the magnetic sleeve 59 by the magnetic rod 54, and the plasmid is extracted by the magnetic beads, and finally the magnetic rod 54 and the magnetic sleeve 59 rise together.

[0039] Step 6: The magnetic rod and magnetic sleeve component 5 moves to the top of the hole containing the washing liquid in the washing area 21, and the magnetic rod 54 and the magnetic sleeve 59 are synchronously extended into the hole containing the washing liquid. The magnetic sleeve 59 and the magnetic rod 54 move up and down synchronously in the washing liquid, so that the magnetic beads are washed in the washing liquid to remove impurities as much as possible;

[0040] Step 7: Move the magnetic rod and magnetic sleeve component 5 to above the hole containing the eluent in the elution zone 22, and the magnetic rod 54 and the magnetic sleeve 59 are simultaneously extended into the hole containing the eluent, and the plasmid DNA is eluted from the magnetic beads by the eluent. Finally, the liquid obtained in the eluent hole is the target plasmid, thereby completing the extraction and purification of Escherichia coli plasmid DNA.

[0041] The utility model can automatically complete the steps of liquid injection, oscillation, magnetic bead extraction, cleaning, elution and the like when extracting and purifying Escherichia coli plasmid DNA. The entire extraction and purification process does not require human intervention, thus saving labor costs and improving work efficiency. Furthermore, since the entire extraction and purification process does not require human intervention, the risk of reagent contamination due to improper human operation is reduced.

[0042] The present utility model is not limited to the above-mentioned optimal implementation mode. Anyone can derive other various forms of products under the inspiration of the present utility model. However, no matter what changes are made in the shape or structure, all those having the same or similar technical solutions as the present application fall within the protection scope of the present utility model.

Claims

1. A fully automatic plasmid purification instrument, characterized in that: The invention comprises a base, on which are arranged an oscillating device, a reagent strip device, a first moving device and a liquid injection device; the reagent strip device is provided with a washing area and an elution area; the liquid injection device is used to inject reagents into holes on the oscillating device; the first moving device is provided with a magnetic rod and a magnetic sleeve component; the magnetic rod and the magnetic sleeve component comprises a moving frame connected to the first moving device; the moving frame is provided with a liftable magnetic sleeve and a magnetic rod; the magnetic rod can extend into the magnetic sleeve; the moving frame is also provided with a breaking device.

2. A fully automatic plasmid purification instrument according to claim 1, characterized in that: The movable frame is provided with a guide rail, on which a first lifting seat and a second lifting seat are slidably connected, the first lifting seat is connected with a first lifting plate, and a magnetic rod is installed at the lower end of the first lifting plate; the second lifting seat is connected with a second lifting plate, the second lifting plate is located below the first lifting plate, and a magnetic sleeve is installed at the lower end of the second lifting plate and corresponds to the magnetic rod one by one; the movable frame is provided with a first lifting drive device for driving the first lifting plate to move up and down, and a second lifting drive device for driving the second lifting plate to move up and down.

3. A fully automatic plasmid purification instrument according to claim 2, characterized in that: The first lifting drive device includes a magnetic rod lifting motor arranged on the moving frame, a magnetic rod lifting screw connected to the magnetic rod lifting motor, and the magnetic rod lifting screw is threadedly matched with the first lifting seat.

4. A fully automatic plasmid purification instrument according to claim 2, characterized in that: The second lifting drive device includes a magnetic sleeve lifting motor arranged on the moving frame, a magnetic sleeve lifting screw connected to the magnetic sleeve lifting motor, and the magnetic sleeve lifting screw is threadedly matched with the second lifting seat.

5. A fully automatic plasmid purification instrument according to any one of claims 1 to 4, characterized in that: The breaking device includes a guide frame, a third lifting plate, and a cutter head mounting plate. The guide frame is fixedly arranged on the movable frame, a guide rod is arranged on the lifting plate, the guide rod and the guide frame are slidably matched, the cutter head mounting plate is connected to the lower end of the guide rod, a cutter head is arranged at the lower end of the cutter head mounting plate, and a third lifting drive device for driving the third lifting plate to move up and down is arranged on the guide frame.

6. A fully automatic plasmid purification instrument according to claim 5, characterized in that: The third lifting drive device includes a tool head lifting motor arranged on the guide frame, the tool head lifting motor is connected to a tool head lifting screw, a screw nut is arranged on the third lifting plate, and the tool head lifting screw is threadedly matched with the screw nut.

7. A fully automatic plasmid purification instrument according to any one of claims 1 to 4, characterized in that: The liquid injection device comprises a second moving device, a liquid injection substrate is arranged on the second moving device, and a liquid injection head is arranged on the liquid injection substrate.

8. A fully automatic plasmid purification instrument according to claim 7, characterized in that: A liquid collecting tank is provided at one end of the second moving device.

9. A fully automatic plasmid purification instrument according to any one of claims 1 to 4, characterized in that: The magnetic rod magnetic sleeve component also includes an anti-drip device, which includes an anti-drip tray and a tray motor. The anti-drip tray is slidably connected to the mobile frame, and the tray motor is arranged on the mobile frame. The tray motor is connected to a screw rod, which is threadedly matched with the anti-drip tray.

Images