Stirring mechanism of bioreactor

Abstract

The utility model discloses a stirring mechanism of a bioreactor, which comprises a stirring machine and a liquid preparation bag, a flange block is fixed on the liquid preparation bag, the stirring machine is clamped with the flange block, the stirring machine is detachably connected with a stirring shaft, the stirring shaft penetrates through a shaft hole of the stirring machine and the flange block and then is clamped with a stirring paddle in the liquid preparation bag, and the stirring paddle is clamped with the flange block. The stirring shaft comprises a plurality of sections of shaft rods, every two adjacent shaft rods are in threaded connection, and flat keys and pin holes are formed in the end parts of the shaft rods. Compared with the prior art, the stirring shaft is arranged in the tank body from top to bottom, the stirring shaft is divided into a plurality of sections, the sections can be arranged in sections when the stirring shaft is arranged, the shaft rod can be clamped and fixed by the bolt when the shaft rod is put down, and a large amount of force can be reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of bioreactors, in particular to a stirring mechanism of a bioreactor. Background Art

[0002] After the bioreactor is finished working, the mixed liquid bag needs to be removed and a new liquid bag needs to be installed. During assembly, the stirring paddle in the liquid bag needs to be fixed together with the stirring shaft of the mixer. For large-capacity liquid bags, a longer stirring shaft is required. It is difficult and physically demanding to insert a stirring shaft that is too long into the liquid bag and assemble it with the stirring paddle or take it out of the liquid bag. Utility Model Content

[0003] The utility model provides a stirring mechanism of a bioreactor, which is used to solve the problem that the disassembly and assembly of a stirring shaft and a stirring paddle consumes physical effort.

[0004] The utility model provides a stirring mechanism of a bioreactor, comprising a stirrer and a liquid preparation bag, wherein a flange block is fixed on the liquid preparation bag, the stirrer is clamped with the flange block, a stirring shaft is detachably connected to the stirrer, the stirring shaft passes through an axial hole of the stirrer and the flange block and is clamped with a stirring paddle in the liquid preparation bag, the stirring shaft comprises a multi-section shaft rod, two adjacent shaft rods are threadedly connected, and a flat key and a pin hole are arranged at the end of the shaft rod.

[0005] Preferably, the flange block comprises: a sleeve, a flange shell, a first bearing and a flange self-locking shell, the sleeve is arranged in the flange shell, the first bearing is arranged between the sleeve and the flange shell, and the flange self-locking shell is clamped with the flange shell and arranged below the first bearing.

[0006] Preferably, the flange block further includes a second bearing and a sealing member, wherein the second bearing is located above the first bearing, and the sealing member is disposed between the sleeve and the flange housing and is located above the second bearing.

[0007] Preferably, a cover shell is hinged on the mixer, the cover shell is fixed to the mixer body by a latch, the mounting cover is located inside the cover shell, and the latch pin is matched with a pin hole on the shaft rod.

[0008] Preferably, the tightening direction of the stirring shaft is the same as the rotation direction of the stirrer.

[0009] Preferably, a shaft sleeve is rotatably provided on the mixer, and the shaft hole is provided in the shaft sleeve.

[0010] Preferably, the mixer is threadedly connected to a mounting cover at the shaft sleeve, and the mounting cover can prevent the mixer shaft from moving upward when rotating.

[0011] Preferably, the lower end of the mixer is provided with a first clamping hole adapted to the flange block, the first clamping hole is provided with a notch for the flange block to pass through, a stopper is rotatably arranged at the notch, one end of the stopper is clamped with the mixer body, and the other end is fixed to the mixer body by a buckle.

[0012] Preferably, a sleeve is provided between the flange block and the stirring paddle, and the stirring shaft passes through the sleeve and is clamped with the stirring paddle.

[0013] Preferably, a bayonet pin is provided at the lower end of the stirring shaft, and a second bayonet hole matching with the bayonet pin is provided at the upper end of the stirring paddle.

[0014] Compared with the prior art, the sleeve in the utility model is fixed to the flange block and the stirring paddle respectively, and the flange block is welded to the liquid bag on the outside, so that when the stirring shaft is loaded from top to bottom, the sleeve can be limited so that the stirring shaft and the stirring paddle in the liquid bag can be assembled successfully in one step. The stirring shaft is loaded into the tank body from top to bottom, and the stirring shaft is divided into multiple sections, which can be loaded in sections. When the shaft is lowered, it can be fixed with a latch, which can reduce a lot of effort. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 It is a structural schematic diagram of the liquid preparation bag of the utility model;

[0018] Figure 3 It is a structural schematic diagram of the flange block of the utility model;

[0019] Figure 4 It is an exploded schematic diagram of the flange block of the utility model;

[0020] Figure 5 It is a partial structural schematic diagram of the utility model;

[0021] Figure 6 It is a structural schematic diagram of the shaft rod of the utility model when it is installed;

[0022] Figure 7 for Figure 6 A magnified schematic diagram of the structure at center A;

[0023] Figure 8It is a structural schematic diagram of the shaft rod of the utility model.

[0024] Reference numerals:

[0025] 1. mixer, 11. mixing shaft, 12. bushing, 13. mounting cover, 14. cover shell, 15. mixer body, 16. latch, 17. first clamping hole, 19. stopper, 111. shaft, 1111. flat key, 112. latch, 100. guide surface, 200. sealing groove, 2. liquid preparation bag, 21. flange block, 22. mixing paddle, 23. sleeve, 211. flange shell, 212. sleeve, 213. first bearing, 214. flange self-locking shell, 215. second bearing, 216. sealing element. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the utility model clearer, the technical solution of the utility model will be clearly and completely described below in conjunction with the drawings in the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] The utility model provides a stirring mechanism using the above-mentioned method for assembling a stirring shaft and a liquid preparation bag of a bioreactor, comprising a stirring machine 1 and a liquid preparation bag 2, a flange block 21 being fixed on the liquid preparation bag 2, the stirring machine 1 being clamped with the flange block 21, a stirring shaft 11 being detachably connected to the stirring machine 1, the stirring shaft 11 passing through the shaft hole of the stirring machine 1 and the flange block 21 and being clamped with a stirring paddle 22 in the liquid preparation bag 2, the stirring shaft 11 comprising a multi-section shaft rod 111, two adjacent shaft rods 111 being threadedly connected, and a flat key 1111 and a pin hole being arranged at the end of the shaft rod 111.

[0028] As another embodiment of the utility model: the flange block 21 includes a sleeve 212, a flange housing 211, a first bearing 213 and a flange self-locking shell 214, the sleeve 212 is arranged in the flange housing 211, the first bearing 213 is arranged between the sleeve 212 and the flange housing 211, the flange self-locking shell 214 is clamped with the flange housing 211 and arranged below the first bearing 213, and the flange self-locking shell 214 is used to support the sleeve 212. Specifically, a sealing groove 200 for installing a sealing ring is provided on the flange self-locking shell 214. The flange self-locking shell 214 and the sleeve 212 are sealed by the sealing ring, and the flange self-locking shell 214 and the flange housing 211 are sealed.

[0029] As another embodiment of the present invention, the flange block 21 further includes a second bearing 215 and a seal 216 . The second bearing 215 is located above the first bearing 213 . The seal 216 is disposed between the sleeve 212 and the flange housing 211 and is located above the second bearing 215 .

[0030] As another embodiment of the present invention: Figure 7 A cover 14 is hinged on the mixer 1, and the cover 14 is fixed to the mixer body 15 through a latch 16. The mounting cover 13 is located in the cover 14, and the latch 16 is matched with the pin hole on the shaft 111. The shaft 111 and the cover 14 share one latch 16.

[0031] Specifically, the pin hole is arranged on the flat key 1111 .

[0032] As another embodiment of the present invention, the tightening direction of the stirring shaft 11 is the same as the rotation direction of the stirrer 1 . This design is conducive to ensuring the reliability of the stirring shaft 11 during rotation.

[0033] As another embodiment of the present utility model: a shaft sleeve 12 is rotatably provided on the mixer 1 , and a shaft hole is provided in the shaft sleeve 12 .

[0034] As another embodiment of the present invention: the mixer 1 is threadedly connected with a mounting cover 13 at the shaft sleeve 12. The mounting cover 13 is provided to prevent the mixer shaft 11 from moving upward when rotating.

[0035] As another embodiment of the present utility model: the lower end of the mixer 1 is provided with a first clamping hole 17 adapted to the flange block 21, the first clamping hole 17 is provided with a notch for the flange block 21 to pass through, and a stopper 19 is rotatably arranged at the notch, one end of the stopper 19 is clamped with the mixer body 15, and the other end is fixed to the mixer body 15 by a buckle.

[0036] As another embodiment of the present invention: a sleeve 23 is provided between the flange block 21 and the stirring paddle 22, and the stirring shaft 11 passes through the sleeve 23 and is engaged with the stirring paddle 22. In this process, the sleeve 23 plays a limiting role so that the stirring shaft 11 can quickly connect with the stirring paddle 22.

[0037] As another embodiment of the present invention: a bayonet 112 is provided at the lower end of the stirring shaft 11, and a second clamping hole matching with the bayonet 112 is provided at the upper end of the stirring paddle 22, and the stirring shaft 11 and the stirring paddle 22 are fixed by inserting the bayonet 112 into the second clamping hole.

[0038] As another embodiment of the present invention: Figure 8 A guide surface 100 is provided at the lower end of the latch pin 112 to guide the stirring shaft 11 into the second latch hole of the stirring paddle 22 .

[0039] The utility model provides an assembly process of a stirring mechanism of a bioreactor, comprising the following steps:

[0040] Step 1: Refer to the attached Figure 3 and attached Figure 4 , pass the sleeve 212 through the bearing and install it into the flange housing 211; specifically, there are two bearings, namely the first bearing 213 and the second bearing 215, install the first bearing 213 on the sleeve 212, and then install the sleeve 212 from bottom to top in the flange housing 211, the upper end surface of the first bearing 213 is connected to the flange housing 211, and the lower end surface of the first bearing 213 is connected to the sleeve 212;

[0041] Step 2: Seal and clamp the flange self-locking shell 214 at the lower end of the flange shell 211 to support the sleeve 212; specifically, install the flange self-locking shell 214 into the bottom of the flange shell 211 from bottom to top, and clamp the lower end of the flange self-locking shell 214 in the flange shell 211. This structural design can quickly install the flange self-locking shell 214, so that the sleeve 212, the first bearing 213, the flange shell 211 and the flange self-locking shell 214 are connected as a whole. Before assembly, two sealing rings are installed in the sealing groove 200 of the flange self-locking shell 214, one sealing ring is used to seal the flange self-locking shell 214 and the flange shell 211, and the other sealing ring is used to seal the flange self-locking shell 214 and the sleeve 212.

[0042] Step 3: Refer to the attached Figure 2 , after the two ends of the sleeve 23 are sealed and connected to the sleeve 212 and the stirring paddle 22 respectively, the flange housing 211 is fixed to the liquid preparation bag 2; specifically, one end of the sleeve 23 is put on the stepped interface of the sleeve 212 and tightly sealed with a cable tie, and the other end is put on the stirring paddle 22 and tightly sealed with a cable tie. Then the sleeve 23 is connected to the stirring paddle 22 and put into the liquid preparation bag 2 together, and finally the flange housing 211 is fixed to the liquid preparation bag 2 by welding.

[0043] Step 4: Refer to the attached Figure 1, put the liquid bag 2 into the tank, snap the flange shell 211 with the mixer 1, and make the stirring shaft 11 pass through the mixer 1, the sleeve 212 and the sleeve 23 from top to bottom in sequence and snap with the stirring paddle 22, then tighten the mounting cover 13 to prevent the stirring shaft 11 from moving upward when rotating, and complete the assembly of the liquid bag 2 and the stirring shaft 11 of the bioreactor. The specific steps of snapping the flange shell 211 with the mixer 1 are: open the buckle and rotate the block 19, then install the flange shell 211 along the gap opened by the block 19 into the mixer 1, rotate the block 19 to seal the gap, and then snap the block 19 with the buckle. In the process of snapping the stirring shaft 11 with the stirring paddle 22, the sleeve 23 plays a limiting role, so that the stirring shaft 11 will not be eccentric, and can be quickly assembled with the stirring paddle 22.

[0044] Among them, the following steps are also included between step 2 and step 3: the second bearing 215 is installed between the sleeve 212 and the flange housing 211, the second bearing 215 is located above the first bearing 213, and then the seal 216 is installed above the second bearing 215 to seal the sleeve 212 and the flange housing 211, so as to complete the assembly of the flange block 21. Specifically, the seal 216 is a lip seal.

[0045] In step 4, refer to the attached Figure 5 and attached Figure 6 The stirring shaft 11 is divided into multiple shaft rods 111. First, insert one shaft rod 111 into the mixer 1, then insert the latch 16 into the shaft rod 111 exposed outside the mixer 1 to prevent it from moving downward, and finally screw another shaft rod 111 with the shaft rod 111 exposed outside the mixer 1 and insert it into the mixer 1. Repeat the above steps until all shaft rods 111 are inserted into the mixer 1. At this time, all shaft rods 111 are assembled into the stirring shaft 11, and the stirring shaft 11 is also fixed to the stirring paddle 22. By providing the latch 16, the assembly of the two shaft rods 111 can be facilitated.

[0046] In step 4, the two sections of the shaft rod 111 are tightened by hand, and then the shaft rod 111 is tightened again by using a wrench by turning the flat key 1111 on the shaft rod 111.

[0047] In step 4, the tightening direction of the stirring shaft 11 is the same as the rotation direction of the stirrer 1. This design is conducive to ensuring the reliability of the stirring shaft 11 during rotation.

[0048] In step 4 , after the flange housing 211 is engaged with the mixer 1 , the latch 16 on the mixer 1 is removed, the cover 14 on the mixer 1 is opened, and then the mounting cover 13 is unscrewed to expose the shaft hole on the mixer 1 .

[0049] During the process of engaging the stirring shaft 11 with the stirring paddle 22 , the bottom end of the stirring shaft 11 and the opening of the stirring paddle 22 both adopt a four-sided flat key 1111 with an inclined roundness to ensure that the stirring shaft 11 and the stirring paddle 22 can be perfectly fitted and positioned during assembly.

[0050] In the utility model, the stirring shaft 11 is installed into the tank body from top to bottom. The stirring shaft 11 is divided into multiple sections. The stirring shaft 11 can be installed in sections during installation, which can reduce a lot of effort. The shafts 111 are connected by left-handed threads, and the direction of the threads is consistent with the stirring direction of the stirring shaft 11, which ensures the reliability during rotation. The upper end of each section of the shaft 111 is provided with a pin hole and a flat key 1111, and the pin hole matches the latch 16 on the cover 14. When the first section of the shaft 111 is lowered, it can be clamped and fixed with the latch 16, which saves effort and facilitates the subsequent tightening and installation of the shaft 111. The flange shell 211, the sleeve 212, the first bearing 213, the flange self-locking shell 214, the second bearing 215 and the seal 216 can be quickly assembled into the flange block 21. The sleeve 23 is fixed to the flange block 21 and the stirring paddle 22 respectively. The outside of the flange block 21 is welded to the liquid preparation bag 2. When the stirring shaft 11 is installed from top to bottom, the sleeve 23 can be limited to allow the stirring shaft 11 and the stirring paddle 22 in the liquid preparation bag 2 to be successfully assembled in one step.

[0051] The utility model can be applied to disposable bioreactor systems of different volumes, and provides a convenient and effective disassembly and assembly method for the disposable bioreactor system when the bag needs to be disassembled and assembled, thereby reducing a large amount of time and cost in culturing cells in the disposable bioreactor.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the utility model.

Claims

1. A stirring mechanism for a bioreactor, characterized in that: The invention comprises a mixer (1) and a liquid preparation bag (2), wherein a flange block (21) is fixed on the liquid preparation bag (2), the mixer (1) is clamped with the flange block (21), a mixing shaft (11) is detachably connected to the mixer (1), the mixing shaft (11) passes through the shaft hole of the mixer (1) and the flange block (21), and is clamped with a mixing paddle (22) in the liquid preparation bag (2), the mixing shaft (11) comprises a multi-section shaft rod (111), two adjacent shaft rods (111) are threadedly connected, and a flat key (1111) and a pin hole are provided at the end of the shaft rod (111).

2. The stirring mechanism of the bioreactor according to claim 1, characterized in that: The flange block (21) comprises: a sleeve (212), a flange shell (211), a first bearing (213) and a flange self-locking shell (214); the sleeve (212) is arranged in the flange shell (211); the first bearing (213) is arranged between the sleeve (212) and the flange shell (211); and the flange self-locking shell (214) is clamped with the flange shell (211) and arranged below the first bearing (213).

3. The stirring mechanism of the bioreactor according to claim 2, characterized in that: The flange block (21) further comprises a second bearing (215) and a sealing member (216), wherein the second bearing (215) is located above the first bearing (213), and the sealing member (216) is arranged between the sleeve (212) and the flange housing (211) and is located above the second bearing (215).

4. The stirring mechanism of the bioreactor according to claim 1, characterized in that: The mixer (1) is hinged with a cover (14), the cover (14) is fixed to the mixer body (15) via a latch (16), the mounting cover (13) is located inside the cover (14), and the latch (16) is matched with a pin hole on the shaft (111).

5. The stirring mechanism of the bioreactor according to claim 1, characterized in that: The tightening direction of the stirring shaft (11) is the same as the rotation direction of the stirring machine (1).

6. The stirring mechanism of the bioreactor according to claim 4, characterized in that: A shaft sleeve (12) is rotatably arranged on the mixer (1), and the shaft hole is arranged in the shaft sleeve (12).

7. The stirring mechanism of the bioreactor according to claim 5, characterized in that: The mixer (1) is threadedly connected to a mounting cover (13) at the shaft sleeve (12), and the mounting cover (13) can prevent the mixer shaft (11) from moving upward when rotating.

8. The stirring mechanism of the bioreactor according to claim 7, characterized in that: The lower end of the mixer (1) is provided with a first clamping hole (17) adapted to the flange block (21); the first clamping hole (17) is provided with a notch (18) for the flange block (21) to pass through; a stopper (19) is rotatably arranged at the notch (18); one end of the stopper (19) is clamped with the mixer body (15), and the other end is fixed to the mixer body (15) by a buckle.

9. The stirring mechanism of the bioreactor according to claim 8, characterized in that: A sleeve (23) is provided between the flange block (21) and the stirring paddle (22), and the stirring shaft (11) passes through the sleeve (23) and is clamped with the stirring paddle (22).

10. The stirring mechanism of the bioreactor according to claim 9, characterized in that: A latch pin (112) is provided at the lower end of the stirring shaft (11), and a second latch hole matching the latch pin (112) is provided at the upper end of the stirring paddle (22).

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