Device for sampling of fermentation tank
By designing a device for fermenter sampling, including a watering can bottle, a bracket and a removable and installed centrifuge tube, the problem of two people requiring cooperation in fermenter sampling is solved, and the sampling is completed independently by a single person, and labor costs and pollution risks are reduced.
Patent Information
- Application Number
- CN202421291898.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-06
- Publication Date
- 2025-03-21
- Estimated Expiration
- 2034-06-06
AI Technical Summary
During the microbial fermentation process, the fermenter sampling requires two people to cooperate, resulting in high labor costs and a risk of pollution.
A device including a watering can bottle, a bracket and a removable mounted centrifuge tube is designed to spray alcohol through the spray head and use plastic abutment wire and adjustment components to achieve independent sampling of a single person.
The sampling can be completed without the cooperation of two people, reducing labor costs and reducing the risk of contamination by reducing the contact time between the hands and the centrifuge tube.
Smart Images
Figure CN222647976U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of fermentation tank sampling, and in particular to a device used for fermentation tank sampling. Background Art
[0002] In the expansion and production of microorganisms, fermenters are usually required. Fermentation tank sampling is the first step to understand the fermentation status of microorganisms.
[0003] Fermentation tanks are usually equipped with special sampling ports. Since sampling of fermentation tanks is carried out in an open environment, in order to prevent contamination caused by sampling, the sampling work of fermentation tanks generally requires one person to hold the centrifuge tube and operate the sampling valve for sampling, and another person to hold the alcohol spray bottle to assist in spraying 75% alcohol during the entire operation. When large-scale continuous sampling is carried out, two people are always required to cooperate to complete it, which has a high labor cost. Utility Model Content
[0004] In order to help reduce labor costs, the present application provides a device for sampling a fermentation tank.
[0005] The present application provides a device for sampling a fermentation tank using the following technical solution:
[0006] A device for sampling a fermentation tank comprises a spray bottle for containing alcohol, the spray bottle having a spray head, the spray bottle being provided with a bracket on which a centrifuge tube is detachably mounted, and the spray head being arranged to tilt downward toward the position of the centrifuge tube on the bracket.
[0007] Preferably, the bracket is provided with a placement tube, the placement tube is used for the centrifuge tube to be moved in or out, and the placement tube is provided with a fixing piece, the fixing piece is used to fix the centrifuge tube and the placement tube relatively.
[0008] Preferably, the fixing member comprises a clamping bolt threadedly provided on the placement tube, the rotation axis of the clamping bolt is perpendicular to the axial direction of the centrifuge tube, and the clamping bolt is used to abut against the outer wall of the centrifuge tube.
[0009] Preferably, the placement tube is arranged at an angle, and the distance from the placement tube to the center line of the spray bottle decreases from top to bottom.
[0010] Preferably, a plastic abutment wire is slidably penetrated on the bracket, and the end of the abutment wire close to the centrifuge tube is used to abut the tube cover of the centrifuge tube from bottom to top and rotate in a direction away from the tube mouth. The bracket is provided with an adjustment component, and the adjustment component is used to adjust the end of the abutment wire close to the centrifuge tube to move from bottom to top or from top to bottom.
[0011] Preferably, the adjustment assembly includes an adjustment handle hinged on the bracket and a reset member arranged on the bracket, the end of the abutment wire away from the centrifuge tube is arranged on the adjustment handle, and the reset member is used to drive the adjustment handle to rotate for reset, and when the adjustment handle is rotated in a direction close to the end of the abutment wire, the adjustment handle pushes the end of the abutment wire close to the centrifuge tube to move from bottom to top.
[0012] Preferably, the reset member comprises a torsion spring for driving the adjustment handle to rotate for reset, one end of the torsion spring is arranged on the bracket, and the other end is arranged on the adjustment handle.
[0013] Preferably, a protrusion is provided at one end of the abutment wire close to the centrifuge tube, and the protrusion is used to abut against a surface of the centrifuge tube cover close to the tube body.
[0014] Preferably, the head of the tightening bolt is configured to be butterfly-shaped.
[0015] Preferably, the distance from the side of the placement tube away from the spray bottle to the outer wall of the spray bottle is 8cm-10cm.
[0016] In summary, this application includes the following beneficial technical effects:
[0017] Install the sterilized and capped centrifuge tube on the bracket, hold the spray bottle in one hand to move the centrifuge tube to the sampling port of the fermentation tank, remove the cap of the centrifuge tube, align the centrifuge tube with the sampling port, and use the other hand to rotate the sampling valve to release the liquid for sampling. During the sampling process, the hand holding the spray bottle presses the spray handle on the nozzle at the same time, so that the nozzle continuously sprays alcohol to sterilize the sampling environment. The entire sampling process does not require the cooperation of two people, and can be completed independently by one person, which helps to reduce labor costs. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application.
[0019] Figure 2 It is a partial structural cross-sectional view of an embodiment of the present application.
[0020] Figure 3 yes Figure 2 Enlarged view of part A.
[0021] Explanation of the reference numerals: 1. spray bottle; 101. spray head; 2. bracket; 3. centrifuge tube; 4. placement tube; 5. tightening bolt; 6. abutment wire; 7. adjustment handle; 8. torsion spring; 9. bump; 10. channel; 11. support block; 12. rotating shaft. DETAILED DESCRIPTION
[0022] The following is combined with Figure 1-3 This application is described in further detail.
[0023] The present application embodiment discloses a device for sampling a fermentation tank. Figure 1 The device for sampling a fermentation tank includes a spray bottle 1 for containing alcohol, the cross-section of the spray bottle 1 is circular, the height of the spray bottle 1 is 9 cm, and the diameter is 6 cm; the spray bottle 1 has a spray head 101, the vertical length of the spray head 101 is 7 cm, and the spray head 101 is provided with a spray handle, wherein the specific structures of the spray head 101 and the spray handle belong to the prior art and will not be elaborated herein.
[0024] Reference Figure 1 and Figure 2 A bracket 2 is fixedly arranged on the outer wall of the bottle body of the spray bottle 1, and the longitudinal section of the bracket 2 is L-shaped. The bracket 2 extends in the direction away from the spray bottle 1, and a placement tube 4 is fixed on the end of the bracket 2 away from the spray bottle 1. The upper surface of the placement tube 4 is lower than the upper surface of the bracket 2 near one end, and a centrifuge tube 3 is detachably installed in the placement tube 4, and the nozzle of the centrifuge tube 3 is upward, and the nozzle of the nozzle 101 is tilted downward toward the position of the centrifuge tube 3 on the bracket 2; the distance from the side of the placement tube 4 away from the spray bottle 1 to the outer wall of the spray bottle 1 is 8cm-10cm, preferably 9cm, so that the nozzle 101 is not easy to interfere with the fermentation tank during sampling; the distance from the side of the placement tube 4 away from the spray bottle 1 to the outer wall of the spray bottle 1 is greater than the horizontal distance from the side of the nozzle 101 away from the spray bottle 1 to the outer wall of the spray bottle 1, which helps to make the alcohol sprayed by the nozzle 101 cover the environment around the sampling.
[0025] When in use, fill 75% alcohol into the spray bottle 1, then install the sterilized and capped centrifuge tube 3 in the placement cylinder 4 on the bracket 2 on the clean bench, then hold the spray bottle 1 with one hand to drive the centrifuge tube 3 to move to the sampling port of the fermentation tank, remove the tube cover of the centrifuge tube 3, align the centrifuge tube 3 with the sampling port, and use the other hand to rotate the sampling valve to release liquid for sampling. During the sampling process, the hand holding the spray bottle 1 continuously presses the spray handle on the nozzle 101, so that the nozzle 101 continuously sprays alcohol to sterilize the sampling environment. After the sampling is completed, the sampling valve and the tube cover of the centrifuge tube 3 are closed to achieve sampling. The entire sampling process does not require the cooperation of two people, and one person can complete it independently, which helps to reduce labor costs, is simple to operate, and is light and convenient; at the same time, it reduces the contact time between the hand and the centrifuge tube 3, which helps to reduce possible sources of pollution.
[0026] Reference Figure 1 The placement tube 4 is tilted, and the distance from the placement tube 4 to the center line of the spray bottle 1 decreases from top to bottom, so that the centrifuge tube 3 installed in the placement tube 4 is tilted, which helps the centrifuge tube 3 to dock with the tilted sampling port on the fermentation tank, and is more practical.
[0027] Reference Figure 1 and Figure 2The placement tube 4 is a cylinder with an opening at the upper end and a closed lower end. The placement tube 4 is used for the centrifuge tube 3 to be moved in or out. A fixing piece is provided on the placement tube 4, and the fixing piece is used to fix the centrifuge tube 3 relatively to the placement tube 4; in order to facilitate the relative fixation of the centrifuge tube 3 and the placement tube 4, the fixing piece includes a tightening bolt 5, and a plurality of tightening bolts 5 are provided. The plurality of tightening bolts 5 are arranged at intervals along the length direction of the placement tube 4. The tightening bolt 5 is threadedly penetrated on the placement tube 4, and the rotation axis of the tightening bolt 5 is perpendicular to the axial direction of the centrifuge tube 3. The tightening bolt 5 is used to abut the outer wall of the centrifuge tube 3; the head of the tightening bolt 5 is set to a butterfly shape, and the butterfly shape adopts the form of a key handle, so that it is convenient for the experimenter to rotate the tightening bolt 5.
[0028] When the centrifuge tube 3 needs to be installed in the placement cylinder 4, the centrifuge tube 3 is placed in the placement cylinder 4 so that the tube mouth of the centrifuge tube 3 is close to the upper end of the placement cylinder 4, and then the plurality of tightening bolts 5 are rotated in sequence to make the tightening bolts 5 abut against the outer wall of the centrifuge tube 3, and the centrifuge tube 3 is pressed against the inner wall of the placement cylinder 4, and the relative fixation of the centrifuge tube 3 and the placement cylinder 4 is achieved by relying on the friction force between the inner walls of the centrifuge tube 3 and the placement cylinder 4; when the centrifuge tube 3 needs to be removed, the plurality of tightening bolts 5 are rotated in sequence to make the tightening bolts 5 disengage from the outer wall of the centrifuge tube 3, and the centrifuge tube 3 can be taken out of the placement cylinder 4 at this time, and the disassembly of the centrifuge tube 3 and the placement cylinder 4 is achieved, and the operation is simple and convenient.
[0029] Reference Figure 1 and Figure 2 , a plastic abutment wire 6 is slidably penetrated on the bracket 2. In the embodiment of the present application, the abutment wire 6 is an iron wire. In other embodiments, the abutment wire 6 can also be a steel wire, an aluminum wire, etc.; one end of the abutment wire 6 is close to the upper end of the placement tube 4, and the other end is close to the spray bottle 1. A channel 10 for the abutment wire 6 to slide through is opened in the bracket 2; the end of the abutment wire 6 close to the placement tube 4 passes through the bracket 2 upward and the passing direction is parallel to the inclination direction of the placement tube 4, and a protrusion 9 is fixed on the end of the abutment wire 6 close to the centrifuge tube 3. The protrusion 9 at one end of the abutment wire 6 close to the centrifuge tube 3 is used to abut the tube cover of the centrifuge tube 3 from bottom to top, so that the tube cover rotates in a direction away from the tube mouth, and an adjusting component is provided on the bracket 2. The adjusting component is used to adjust the end of the abutment wire 6 close to the centrifuge tube 3 to move from bottom to top or from top to bottom.
[0030] Reference Figure 2 and Figure 3In order to facilitate the adjustment of the end of the abutment wire 6 close to the centrifuge tube 3 to move from bottom to top or from top to bottom, a support block 11 is integrally formed on the side of the bracket 2 close to the spray bottle 1, and the adjustment component includes an adjusting handle 7 and a reset member. A rotating shaft 12 is fixedly penetrated on the adjusting handle 7, and the rotating shaft 12 is rotatably penetrated on the support block 11. The rotation axis of the rotating shaft 12 is perpendicular to the height direction of the spray bottle 1. The end of the abutment wire 6 away from the centrifuge tube 3 passes through the bracket 2 and is fixed on a side of the adjusting handle 7 close to the spray bottle 1. The reset member is arranged on the support block 11. The reset member is used to drive the adjusting handle 7 to rotate for reset. When the adjusting handle 7 rotates in the direction close to the end of the abutment wire 6, the adjusting handle 7 pushes the end of the abutment wire 6 close to the centrifuge tube 3 to move from bottom to top.
[0031] Reference Figure 2 and Figure 3 In order to facilitate driving the adjusting handle 7 to rotate for resetting, the resetting member includes a torsion spring 8, which is movably sleeved on the rotating shaft 12, one end of the torsion spring 8 is fixed on the supporting block 11, and the other end is fixed on the adjusting handle 7. When the torsion spring 8 is in a natural state, the side of the adjusting handle 7 away from the spray bottle 1 is tilted downward, and the torsion force of the torsion spring 8 is greater than the friction between the abutting wire 6 and the inner wall of the channel 10.
[0032] When the centrifuge tube 3 is installed in the placement cylinder 4, the tube cover of the centrifuge tube 3 is pressed on the protrusion 9; when the tube cover of the centrifuge tube 3 needs to be opened, the adjusting handle 7 is pressed in the direction close to the spray bottle 1 to deform the torsion spring 8, and the adjusting handle 7 pushes the abutment wire 6 to slide in the bracket 2, so that the end of the abutment wire 6 close to the centrifuge tube 3 moves from bottom to top along the inclined direction of the placement cylinder 4, so that the protrusion 9 pushes the tube cover of the centrifuge tube 3 to rotate in the direction away from the tube body of the centrifuge tube 3, and the tube cover of the centrifuge tube 3 is pushed open, and then the adjusting handle 7 is released, and the deformed torsion spring 8 drives the adjusting handle 7 to rotate in the direction away from the spray bottle 1, and the adjusting handle 7 pulls the abutment wire 6, so that the end of the abutment wire 6 close to the centrifuge tube 3 moves from top to bottom along the inclined direction of the placement cylinder 4, so that the protrusion 9 is reset, which is not easy to interfere with the sampling process, is simple and convenient, and does not need to be turned over by hand to open the tube cover of the centrifuge tube 3, further reducing the contact time between the hand and the centrifuge tube 3, which helps to reduce possible sources of pollution.
[0033] The implementation principle of the embodiment of the present application is as follows: when in use, 75% alcohol is filled into the spray bottle 1, and then the sterilized and capped centrifuge tube 3 is placed into the placement cylinder 4 on the bracket 2 on the clean bench, so that the mouth of the centrifuge tube 3 is close to the upper end of the placement cylinder 4, and the tube cover of the centrifuge tube 3 is pressed on the protrusion 9, and the hinge of the tube cover of the centrifuge tube 3 is located on the side of the placement cylinder 4 away from the protrusion 9, and then a plurality of tightening bolts 5 are rotated in sequence to make the tightening bolts 5 abut against the outer wall of the centrifuge tube 3, and the centrifuge tube 3 is pressed against the inner wall of the placement cylinder 4; then one hand holds the spray bottle 1 to drive The centrifuge tube 3 is moved to the vicinity of the sampling port of the fermentation tank, and then the adjusting handle 7 is pressed toward the direction close to the spray bottle 1. The adjusting handle 7 pushes the abutment wire 6 to slide in the bracket 2, so that the end of the abutment wire 6 close to the centrifuge tube 3 moves from bottom to top along the inclined direction of the placement cylinder 4, and the protrusion 9 pushes the tube cover of the centrifuge tube 3 to rotate in the direction away from the tube body of the centrifuge tube 3, thereby pushing the tube cover of the centrifuge tube 3 open, and then the adjusting handle 7 is released, and the deformed torsion spring 8 drives the adjusting handle 7 to rotate in the direction away from the spray bottle 1, and the adjusting handle 7 pulls the abutment wire 6 back to reset.
[0034] Then align the centrifuge tube 3 with the sampling port, and use the other hand to rotate the sampling valve to release the liquid for sampling. During the sampling process, the hand holding the spray bottle 1 continuously presses the spray handle on the nozzle 101 to make the nozzle 101 continuously spray alcohol to sterilize the sampling environment. After the sampling is completed, close the sampling valve and the tube cover of the centrifuge tube 3 to complete the sampling. The entire sampling process does not require the cooperation of two people, and one person can complete it independently, which helps to reduce labor costs; at the same time, it reduces the contact time between the hand and the centrifuge tube 3, which helps to reduce possible sources of contamination.
[0035] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.
Claims
1. A device for sampling a fermentation tank, characterized in that: The invention comprises a spray bottle (1) for containing alcohol, the spray bottle (1) having a spray head (101), the spray bottle (1) being provided with a bracket (2), a centrifuge tube (3) being detachably mounted on the bracket (2), and the spray head (101) being arranged to be tilted downwards towards the position of the centrifuge tube (3) on the bracket (2).
2. A device for sampling a fermentation tank according to claim 1, characterized in that: The support (2) is provided with a placement tube (4), and the placement tube (4) is used for the centrifuge tube (3) to be moved in or out. The placement tube (4) is provided with a fixing piece, and the fixing piece is used to fix the centrifuge tube (3) and the placement tube (4) relative to each other.
3. A device for sampling a fermentation tank according to claim 2, characterized in that: The fixing member comprises a clamping bolt (5) threadedly penetrated on the placement tube (4), the rotation axis of the clamping bolt (5) being perpendicular to the axial direction of the centrifuge tube (3), and the clamping bolt (5) being used for abutting against the outer wall of the centrifuge tube (3).
4. The device for sampling a fermentation tank according to claim 2, characterized in that: The placement tube (4) is arranged obliquely, and the distance from the placement tube (4) to the center line of the spray bottle (1) decreases from top to bottom.
5. A device for sampling a fermentation tank according to any one of claims 1 to 4, characterized in that: The support (2) is provided with a plastic abutment wire (6) which is slidably penetrated, and the end of the abutment wire (6) close to the centrifuge tube (3) is used to abut the tube cover of the centrifuge tube (3) from bottom to top and rotate in a direction away from the tube mouth, and the support (2) is provided with an adjustment component, and the adjustment component is used to adjust the end of the abutment wire (6) close to the centrifuge tube (3) to move from bottom to top or from top to bottom.
6. The device for sampling a fermentation tank according to claim 5, characterized in that: The adjustment assembly comprises an adjustment handle (7) hinged on the bracket (2) and a reset member arranged on the bracket (2); the end of the abutment wire (6) away from the centrifuge tube (3) is arranged on the adjustment handle (7); the reset member is used to drive the adjustment handle (7) to rotate for reset; when the adjustment handle (7) rotates in a direction close to the end of the abutment wire (6), the adjustment handle (7) pushes the end of the abutment wire (6) close to the centrifuge tube (3) to move from bottom to top.
7. The device for sampling a fermentation tank according to claim 6, characterized in that: The reset member comprises a torsion spring (8) for driving the adjustment handle (7) to rotate for reset; one end of the torsion spring (8) is arranged on the bracket (2), and the other end is arranged on the adjustment handle (7).
8. The device for sampling a fermentation tank according to claim 5, characterized in that: A protrusion (9) is provided at one end of the abutment wire (6) close to the centrifuge tube (3), and the protrusion (9) is used to abut against a side of the centrifuge tube (3) cover close to the tube body.
9. The device for sampling a fermentation tank according to claim 3, characterized in that: The head of the tightening bolt (5) is arranged in a butterfly shape.
10. The device for sampling a fermentation tank according to claim 2, characterized in that: The distance between the side of the placement tube (4) away from the spray bottle (1) and the outer wall of the spray bottle (1) is 8 cm to 10 cm.