Viable bacteria culture device

Abstract

The utility model discloses a viable bacterium culture device which comprises a box body, a culture cavity and a ventilation cavity are arranged in the box body, and a culture dish can be placed in the culture cavity; the ventilation cavity and the culture cavity are arranged at an interval and are communicated; the gas detection assembly is used for detecting the gas concentration in the culture cavity; the flow guide assembly is used for guiding gas in the ventilation cavity into the culture cavity or guiding airflow in the culture cavity out; and the degerming assembly is used for degerming. The concentration of gas in the culture cavity can be monitored in real time by the gas detection assembly, the gas in the ventilation cavity can be guided into the culture cavity or guided out of the culture cavity through the flow guide assembly, so that the normal concentration of the gas in the culture cavity is ensured, and due to the fact that the gas in the ventilation cavity is disinfected in real time by the sterilization assembly, the concentration of the gas in the culture cavity is not influenced. Therefore, the gas introduced into the culture cavity is relatively clean, and the risk that the internal environment of the culture cavity is damaged due to gas exchange is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of live bacteria culture, in particular to a live bacteria culture device. Background Art

[0002] Live bacteria culture refers to placing the required bacteria in a culture dish to create a suitable growth environment for them. It is a culture technology for the reproduction and growth of live bacteria. In medical experiments, live bacteria are often cultured in order to better understand the characteristics of bacteria, and the culture of live bacteria is generally carried out in a specific incubator. During the live bacteria culture process, it is necessary to determine and control the concentration of other gases such as oxygen or carbon dioxide according to the characteristics of the cultured live bacteria to ensure that the bacteria can grow normally. Therefore, live bacteria need to exchange gases with the outside during the culture process to maintain the gas concentration inside. The live bacteria culture chambers currently on the market usually directly exchange gases between the outside air and the inside of the incubator. If this part of the air is not disinfected, the internal environment of the incubator will change, affecting the living environment of the live bacteria, which is not conducive to the continued cultivation of live bacteria. Utility Model Content

[0003] In order to overcome at least one of the defects of the above-mentioned prior art, the utility model provides a live bacteria incubator, whose culture chamber can exchange gas with the ventilation chamber. Since the sterilization component inside the ventilation chamber can disinfect the gas inside the ventilation chamber, the risk of the environment inside the culture chamber being damaged during gas exchange can be reduced.

[0004] The technical solution adopted by the utility model to solve the problem is:

[0005] A live bacteria culture device, comprising a box, wherein the box has a culture cavity and a ventilation cavity inside, wherein the culture cavity is used to place a culture dish; one end of the ventilation cavity is spaced apart from and connected to the culture cavity, and the other end of the ventilation cavity is connected to the outside;

[0006] A gas detection component, the gas detection component is located in the culture chamber and is used to detect the gas concentration in the culture chamber;

[0007] A flow guide component, the flow guide component is used to guide the gas in the ventilation cavity to be introduced into the culture cavity, or to guide the airflow in the culture cavity to be discharged from the ventilation cavity;

[0008] A sterilization component is located in the ventilation cavity and is used for sterilization.

[0009] Furthermore, the guide component includes at least two guide fans, the inner wall of the culture chamber is provided with at least two guide holes, at least two of the guide holes are connected to the ventilation chamber, one of the guide fans is used to guide the airflow in the ventilation chamber into the culture chamber through one of the guide holes, and the other guide fan is used to guide the airflow in the culture chamber out of the ventilation chamber through the other guide hole.

[0010] Furthermore, the inner wall of the ventilation cavity is provided with a through hole, which is communicated with an external gas path and is used to introduce or export gas.

[0011] Furthermore, there are two ventilation cavities, the two ventilation cavities are connected to the culture chamber through the two guide holes respectively, and at least two guide fans are respectively located in the two ventilation cavities; the inner walls of the two ventilation cavities are provided with through holes.

[0012] Furthermore, at least two of the flow guide holes are provided with filter valves, and the filter valves are used to filter the airflow.

[0013] Furthermore, the sterilization component includes at least two sterilization lamps, and the at least two sterilization lamps are respectively installed in the ventilation cavity and are used to sterilize the two ventilation cavities respectively.

[0014] Furthermore, a temperature sensor and a heater are provided in the culture chamber. The temperature sensor is used to detect the temperature in the culture chamber, and the heater is used for heating.

[0015] Furthermore, a plurality of support plates are provided in the culture chamber, the plurality of support plates are distributed at intervals in the culture chamber, and are detachably connected to the inner wall of the culture chamber; a plurality of mounting grooves are provided on the plurality of support plates, and the plurality of mounting grooves are used to install culture dishes; an opening is provided on the culture chamber, an openable and closable box cover is provided at the opening, and a light-transmitting plate is provided on the box cover.

[0016] Furthermore, it also includes a lighting lamp, which is located in the culture chamber and is used to release light.

[0017] Furthermore, the gas detection component is a gas detector.

[0018] To sum up, the live bacteria culture device provided by the utility model has the following technical effects: during its specific use, the gas concentration in the culture chamber can be monitored in real time by the gas detection component. When the gas concentration in the culture chamber is detected to be abnormal, the gas can be introduced into the culture chamber through the guide component in the ventilation chamber, or the gas in the culture chamber can be guided out to ensure the normal gas concentration in the culture chamber in real time; in addition, because the sterilization component in the ventilation chamber can disinfect the gas in the ventilation chamber in real time, the gas introduced from the ventilation chamber into the culture chamber is relatively clean, thereby reducing the risk of the internal environment of the culture chamber being destroyed due to gas exchange and improving the survival rate of live bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a structural cross-sectional view of the utility model;

[0020] Figure 2 It is a structural cross-sectional view from another perspective of the utility model;

[0021] Figure 3 It is a structural schematic diagram of the utility model.

[0022] The meanings of the reference numerals are as follows:

[0023] 10. Box body; 11. Culture chamber; 111. Guide hole; 12. Ventilation chamber; 13. Support plate; 131. Mounting slot; 14. Box cover; 15. Control panel; 20. Gas detection component; 30. Guide fan; 40. Sterilization lamp; 50. Lighting lamp; 60. Temperature sensor; 70. Filter valve. DETAILED DESCRIPTION

[0024] For better understanding and implementation, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0025] In the description of the present invention, it should be noted that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0026] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0027] See also Figures 1 to 3 The utility model discloses a living bacteria culture device, including a box body 10, a gas detection component 20, a flow guide component and a sterilization component. A culture chamber 11 and a ventilation chamber 12 are provided inside the box body 10. The culture chamber 11 can be used to place a culture dish. One end of the ventilation chamber 12 is spaced apart from and connected to the culture chamber 11, while the other end of the ventilation chamber 12 is connected to the outside. The gas detection component 20 is installed in the culture chamber 11 to detect the concentration of the gas in the culture chamber 11. Then, when the gas concentration value in the culture chamber 11 is abnormal, the flow guide component can guide the gas in the ventilation chamber 12 to be introduced into the culture chamber 11, or guide the gas in the culture chamber 11 to be discharged, so as to control the gas concentration in the culture chamber 11. In addition, the sterilization component is installed in the ventilation chamber 12 to disinfect and sterilize the gas in the ventilation chamber 12 through the sterilization component.

[0028] On the basis of the above structure, when in use, the bacteria to be cultured can be placed in a culture dish, and the culture dish can be placed in the culture chamber 11. Because the concentration of gas (such as oxygen or carbon dioxide, etc.) required by each bacteria species during the cultivation process is different, and the bacteria species will release waste gas during the cultivation, the bacteria species need to exchange gas with the outside during the cultivation process to ensure that the concentration of oxygen or other gases in the gas in the culture chamber 11 can be adjusted in real time through gas exchange. Therefore, a gas detection component 20 is set in the culture chamber 11, and the gas detection component 20 is used to monitor the concentration of the gas in the culture chamber 11 in real time.

[0029] Specifically, in this embodiment, the gas required by the bacteria in the culture chamber 11 is oxygen. At this time, the gas detection component 20 can be selected as an oxygen detector. When in use, an index value of the oxygen concentration (such as oxygen content) required by the bacteria can be built into the gas detection component 20. When it is detected that the oxygen content in the culture chamber 11 is lower than the set index value, at this time, the gas in the ventilation chamber 12 can be guided into the culture chamber 11 through the guide component to increase the oxygen concentration in the culture chamber 11;

[0030] Similarly, when the gas detection component 20 monitors that the oxygen concentration (oxygen content) in the culture chamber 11 is higher than the index value, the diversion component can be opened again to guide the gas in the culture chamber 11 to be discharged to the ventilation chamber 12, so as to ensure the normal oxygen concentration in the culture chamber 11 in real time and ensure the normal growth of the bacteria in the culture chamber 11.

[0031] Of course, when the gas required for the cultured bacteria in this embodiment is carbon dioxide or other gases, the gas detection component 20 in this embodiment can correspondingly select a gas detector that can detect carbon dioxide, etc., and specifically select the required detector or detector for detecting other gas contents according to actual needs.

[0032] Specifically, the ventilation chamber 12 can be connected to an external air path or directly connected to the external environment, and then a sterilization component is set in the ventilation chamber 12 to sterilize the gas in the ventilation chamber 12. In this way, it can be ensured that the gas introduced into the ventilation chamber 12 is clean and sterile, so as to reduce the risk of destroying the culture environment of the bacteria and preventing them from growing normally due to the gas carrying bacteria when the culture chamber 11 and the ventilation chamber 12 exchange gases.

[0033] It should be noted that the ventilation chamber 12 and the culture chamber 11 can be connected by means of a catheter or an air hole, and a valve (such as a one-way valve or a breathable valve, etc.) for controlling other one-way flows can be set at the catheter or the air hole, so that when the gas concentration in the culture chamber 11 is normal, the valve can be used to block the gas in the ventilation chamber 12 from flowing into the culture chamber 11 in real time, thereby affecting the internal gas concentration.

[0034] When the gas required in the culture chamber 11 is oxygen or other gases with a high content in the air, the ventilation chamber 12 can be directly connected to the outside to introduce air into the ventilation chamber 12 in real time, and then the air inside is sterilized and disinfected by the sterilization component to ensure that the internal gas is sterile, so that the concentration of oxygen or other gases in the culture chamber 11 can be increased after it is transported to the culture chamber 11; of course, the ventilation chamber 12 can also be connected to an external gas tank to transport oxygen or other gases into the ventilation chamber 12 in real time, so as to adjust the concentration of the gas in the culture chamber 11 in real time;

[0035] Of course, when the gas required by the bacteria cultured in the culture chamber 11 is other gases with lower content in the air, the gas introduced into the ventilation chamber 12 can be the gas required by the bacteria. At this time, the ventilation chamber 12 can be connected to a specific external gas tank (the gas filled in the gas tank is the gas required for culturing the bacteria) so that the gas required for culturing the bacteria filled in the ventilation chamber 12 can be introduced into the culture chamber 11.

[0036] It should be noted that the gas detection component 20 in this embodiment is preferably a gas detector, which can be electrically connected to the control panel 15 built into the box 10 during assembly to transmit the detected data values ​​to the control panel 15 in real time for the user to view.

[0037] Preferably, the sterilization component in this embodiment can be an ultraviolet lamp, a UVB disinfection lamp or an air purifier and disinfector, which can be installed on the inner wall of the ventilation cavity 12 by means of connectors such as screws or bolts during installation; the guide component can be an exhaust fan or an exhaust fan and other components used to guide gas flow.

[0038] During assembly, the box body 10 can be formed by connecting and surrounding a plurality of mounting plates, or can be integrally formed, and a culture chamber 11 can be formed inside by setting a plurality of plates to surround the culture chamber 11, and the plurality of plates are spaced apart from the inner wall of the box body 10 so that a ventilation chamber 12 is formed between the plurality of plates and the inner wall of the box body 10; in addition, a shell can be separately set inside the box body 10, and the culture chamber 11 can be integrally formed inside the shell, and then the shell and the inner wall of the box body 10 are spaced apart to form a ventilation chamber 12, and the specific setting can be based on actual needs.

[0039] Furthermore, the guide component includes at least two guide fans 30, and the inner wall of the culture chamber 11 is provided with at least two guide holes 111, which connect the at least two guide holes 111 to the ventilation chamber 12, and one of the guide fans 30 can be used to guide the airflow in the ventilation chamber 12 through one of the guide holes 111 into the culture chamber 11, and the other guide fan 30 is used to guide the airflow in the culture chamber 11 through the other guide hole 111 to the ventilation chamber 12.

[0040] Specifically, in this embodiment, at least two guide holes 111 are provided on the inner wall of the culture chamber 11, and at least two guide fans 30 are provided. When the gas in the culture chamber 11 needs to be discharged, one of the guide fans 30 can be turned on to guide the gas inside to be discharged into the ventilation chamber 12 through one of the guide holes 111, and then discharged to the outside through the ventilation chamber 12. When it is necessary to supplement the gas required for the bacterial strain to be added to the culture chamber 11, another guide fan 30 can be used to guide the gas required for culturing the bacterial strain in the ventilation chamber 12 to be introduced into the culture chamber 11 through another guide hole 111.

[0041] It should be noted that the guide hole 111 for guiding the gas in the culture chamber 11 can be connected to the guide fan 30 through a conduit, and the conduit can be directly extended to the outside of the ventilation chamber 12 to prevent the gas discharged from the culture chamber 11 from mixing with the gas in the ventilation chamber 12, and the structure is more practical.

[0042] In addition, the number of the guide holes 111 can be increased according to the efficiency of discharging or introducing gas as needed, and can be three, four or more, depending on actual needs, and the number of the guide fans 30 can also be increased or decreased according to the number of the guide holes 111.

[0043] More specifically, a filter valve 70 is provided at at least two of the guide holes 111. The filter valve 70 can be blocked at the guide hole 111 and can filter the gas flowing through the guide hole 111, so that the gas in the ventilation cavity 12 is introduced through the guide hole 111 and can be further filtered through the filter valve 70, so that the gas introduced into the culture cavity 11 is purer, so as to further reduce the risk of the bacteria in the culture cavity 11 being destroyed.

[0044] The other air-permeable valve for guiding the gas out of the culture chamber 11 can filter the gas discharged from the culture chamber 11 to prevent the gas directly discharged from the culture chamber 11 from polluting the external environment.

[0045] Preferably, the filter valve 70 can be an exhaust valve or a breathable valve that can control the gas flow direction in one direction, and when the filter valves 70 used at the two guide holes 111 are installed, their internal flow directions can be reversed so that one of the filter valves 70 is used for exhaust and the other filter valve 70 is used for intake.

[0046] Of course, activated carbon filters or the like may be provided at the two guide holes 111 to filter the gas, and then ordinary valves may be provided at the guide holes 111 to control the closing of the guide holes 111 respectively, and the valves may be opened when exhaust or intake is required.

[0047] In addition, a through hole is provided on the inner wall of the ventilation chamber 12, and a plurality of through holes can be provided, and one or more of the through holes are connected to the external air path to introduce the gas required for culturing bacteria into the ventilation chamber 12 through the through hole, while another through hole is connected to the culture chamber 11 through a conduit, etc., so that the gas in the culture chamber 11 can be discharged to the outside.

[0048] On this basis, the guide fan 30 for guiding the gas in the culture chamber 11 to be discharged can be correspondingly arranged at the through hole for discharging the gas to the outside, thereby guiding the gas in the culture chamber 11 to be discharged to the outside through the through hole.

[0049] Preferably, in order to prevent the gas discharged from the culture chamber 11 from mixing with the gas in the ventilation chamber 12 and affecting the concentration of the gas in the ventilation chamber 12 and causing it to be unusable, two ventilation chambers 12 are provided in this embodiment, and the two ventilation chambers 12 are connected to the culture chamber 11 through two guide holes 111 respectively, one of the ventilation chambers 12 can be used to introduce the gas required by the bacteria and discharge it to the culture chamber 11 through the guide holes 111, while the other ventilation chamber 12 can be used to receive the exhaust gas discharged from the culture chamber 11, and two guide fans 30 are respectively arranged in the two ventilation chambers 12 to guide the gas flow respectively.

[0050] Among them, the inner walls of the two ventilation chambers 12 are both provided with through holes. One ventilation chamber 12 used to store the gas required by the bacteria is connected to the external air path to introduce the gas into the ventilation chamber 12, and the through hole on the other ventilation chamber 12 is directly connected to the external environment to discharge the waste gas.

[0051] Furthermore, the above-mentioned disinfection component includes at least two sterilization lamps 40, which are respectively installed in two ventilation chambers 12. The two sterilization lamps are respectively used to sterilize and disinfect the inside of the two ventilation chambers 12, so that the gas introduced into the culture chamber 11 is clean and sterile, and the gas in the culture chamber 11 can be directly discharged into the ventilation chamber 12 for exhaust, and then discharged into the external environment after being disinfected by the sterilization lamp 40, so as to reduce the risk of the exhausted gas polluting the external environment, which is more environmentally friendly.

[0052] Of course, the number of sterilization lamps 40 can also be three, four or more, and multiple sterilization lamps 40 can be used to simultaneously sterilize and disinfect the ventilation cavity 12, thereby achieving a better sterilization effect. Specifically, the sterilization lamp 40 can be an ultraviolet disinfection lamp, etc.

[0053] Furthermore, a temperature sensor 60 and a heater are provided in the culture chamber 11 . The temperature sensor 60 is used to detect the temperature in the culture chamber 11 , and the heater is used to heat the culture chamber 11 when the temperature in the culture chamber 11 is lower than a preset value of the temperature sensor 60 .

[0054] Specifically, since bacterial culture requires a certain temperature, a temperature sensor 60 is also provided in the culture chamber 11 in the present embodiment. By setting the required temperature value of the culture chamber 11 in the temperature sensor 60, when the temperature sensor 60 detects that the temperature value is lower than the preset temperature value, an alarm may be issued, and then the heater may be turned on to heat the inside of the culture chamber 11.

[0055] It should be noted that the temperature sensor 60 and the heater can be electrically connected to the control panel 15 to transmit the internal temperature value to the control panel 15 in real time, and then the start and stop of the heater can be controlled by the control panel 15 .

[0056] Preferably, the heater can be a heating tube or a resistance heating wire.

[0057] Furthermore, a plurality of support plates 13 are provided in the culture chamber 11. When assembled, the plurality of support plates 13 are arranged in the culture chamber 11 at intervals, and are respectively connected to the inner wall of the culture chamber 11 in a detachable connection manner. Specifically, a block-shaped or plate-shaped support portion may be provided on the inner wall of both sides of the culture chamber 11, and the support plate 13 may be directly placed on the block-shaped or plate-shaped structure, or a structure such as a clamping groove may be provided to clamp the support plate 13. When it is necessary to culture multiple strains at the same time, the plurality of support plates 13 may be installed at the same time, and a plurality of mounting grooves 131 may be provided on the plurality of support plates 13 to install the culture dish, so that multiple strains may be cultured at the same time, which is more efficient.

[0058] When a larger culture dish needs to be placed, since the support plate 13 is detachable, part of the support plate 13 can be removed to enlarge the space in the culture chamber 11 to accommodate a larger culture dish, so that the culture chamber 11 can be suitable for culture dishes of different sizes.

[0059] In addition, the culture chamber 11 has an opening, through which the culture dish can be placed into the culture chamber 11, and a closable box cover 14 is provided at the opening. The box cover 14 can cover the opening to form a closed space inside the culture chamber 11, and a light-transmitting plate is provided on the box cover 14, so that after the box cover 14 covers the opening, the situation inside the culture chamber 11 can still be observed through the light-transmitting plate, so as to monitor the growth of the bacteria in real time.

[0060] Preferably, the light-transmitting plate can be made of materials such as glass or transparent plastic.

[0061] Furthermore, the utility model also includes an illumination lamp 50, which can be installed on the top of the culture chamber 11 during assembly, and releases light in dim light conditions to illuminate the interior of the culture chamber 11 for easy observation by outsiders.

[0062] Preferably, the lighting lamp 50 can be an LED lamp or an incandescent lamp.

[0063] The technical means disclosed in the solution of the utility model are not limited to the technical means disclosed in the above-mentioned implementation mode, but also include technical solutions composed of any combination of the above technical features. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the utility model, and these improvements and modifications are also regarded as the protection scope of the utility model.

Claims

1. A living bacteria culture device, characterized in that: It comprises a box body, wherein the box body has a culture cavity and a ventilation cavity inside, wherein the culture cavity is used to place a culture dish; one end of the ventilation cavity is spaced apart from and connected to the culture cavity, and the other end of the ventilation cavity is connected to the outside; A gas detection component, the gas detection component is located in the culture chamber and is used to detect the gas concentration in the culture chamber; A flow guide component, the flow guide component is used to guide the gas in the ventilation cavity to be introduced into the culture cavity, or to guide the airflow in the culture cavity to be discharged from the ventilation cavity; A sterilization component is located in the ventilation cavity and is used for sterilization.

2. The live bacteria culture device according to claim 1, characterized in that: The guide assembly includes at least two guide fans, the inner wall of the culture chamber is provided with at least two guide holes, at least two of the guide holes are connected to the ventilation chamber, one of the guide fans is used to guide the airflow in the ventilation chamber into the culture chamber through one of the guide holes, and the other guide fan is used to guide the airflow in the culture chamber out of the ventilation chamber through the other guide hole.

3. The live bacteria culture device according to claim 2, characterized in that: The inner wall of the ventilation cavity is provided with a through hole, which is communicated with an external air path and is used for introducing or discharging gas.

4. The live bacteria culture device according to claim 3, characterized in that: There are two ventilation cavities, the two ventilation cavities are connected to the culture chamber through the two guide holes respectively, and at least two guide fans are respectively located in the two ventilation cavities; the inner walls of the two ventilation cavities are provided with through holes.

5. The live bacteria culture device according to claim 4, characterized in that: At least two of the flow guide holes are provided with filter valves, and the filter valves are used to filter the airflow.

6. The live bacteria culture device according to claim 4, characterized in that: The sterilization component comprises at least two sterilization lamps, which are respectively installed in the ventilation cavity and used for sterilizing the two ventilation cavities respectively; the sterilization lamps are ultraviolet disinfection lamps.

7. The live bacteria culture device according to claim 1, characterized in that: A temperature sensor and a heater are also provided in the culture chamber. The temperature sensor is used to detect the temperature in the culture chamber, and the heater is used for heating.

8. The live bacteria culture device according to claim 1, characterized in that: A plurality of support plates are arranged in the culture chamber, the plurality of support plates are spaced apart in the culture chamber, and are detachably connected to the inner wall of the culture chamber; a plurality of mounting grooves are arranged on the plurality of support plates, and the plurality of mounting grooves are used for mounting culture dishes; an opening is arranged on the culture chamber, an openable and closable box cover is arranged at the opening, and a light-transmitting plate is arranged on the box cover.

9. The live bacteria culture device according to claim 1, characterized in that: Also included is a lighting lamp, which is located in the culture chamber and is used to release light.

10. The live bacteria culture device according to claim 1, characterized in that: The gas detection component is a gas detector.

Images