Isothermal amplification kit

Abstract

The utility model discloses an isothermal amplification kit, which relates to the technical field of isothermal amplification experiments and comprises a mounting plate, a mounting shell is arranged above the mounting plate, and an inclined mechanism is arranged between the mounting plate and the mounting shell. By combining the rotary motion driven by a servo motor and the tilting action controlled by an electric push rod, the multi-dimensional and all-directional shaking and mixing of a reagent tube in a three-dimensional space are realized, and the mixing efficiency and uniformity between a reagent and a sample are remarkably improved by adopting the mixing mode; specifically, a servo motor drives a rotating shaft and an inclination mechanism connected with the rotating shaft to rotate, meanwhile, an electric push rod accurately controls the inclination angle of a mounting shell through a connecting structure of a second rotating block and a second pin rod, and the electric push rod and the second rotating block act synergistically, so that the mixing efficiency is improved. And the sample and the reagent in the reagent tube can be fully and uniformly mixed.

Description

Technical Field

[0001] The utility model relates to the technical field of isothermal amplification experiments, in particular to an isothermal amplification kit. Background Art

[0002] Isothermal amplification has become an important tool in molecular biology research, particularly in pathogen detection and genetic diagnosis, where its application is growing. However, ensuring adequate mixing of reagents and samples is crucial when conducting isothermal amplification experiments, as this uniformity directly impacts amplification efficiency and specificity, and thus the accuracy and reliability of experimental results.

[0003] Traditional isothermal amplification kits often rely on manual manipulation by the experimenter to mix the reagents and samples, such as through repeated pipetting or vortexing. While these manual methods can achieve a certain mixing effect, they are cumbersome and time-consuming, and uniform mixing is difficult to ensure. This method is particularly inefficient when processing large numbers of samples and is prone to introducing human error, which can affect the accuracy and reproducibility of experimental results.

[0004] To address this issue, some existing isothermal amplification kits attempt to incorporate mechanical vibration platforms to aid in the mixing process. However, these platforms typically employ a single vibration mode, such as up-and-down vibration or horizontal shaking, making it difficult to achieve comprehensive mixing in three dimensions. Consequently, these designs still have limitations in mixing effectiveness and cannot meet the demands of high-precision and high-efficiency experiments. Utility Model Content

[0005] The purpose of the utility model is to make up for the deficiencies of the prior art and to provide an isothermal amplification kit.

[0006] To achieve the above-mentioned objectives, the present invention provides the following technical solutions: an isothermal amplification test kit, comprising a mounting plate, a mounting shell is arranged above the mounting plate, a tilting mechanism is arranged between the mounting plate and the mounting shell, the tilting mechanism comprises a first U-shaped member, a strip, and a second U-shaped member fixedly connected to the bottom surface of the mounting shell, a first pin is fixedly connected to the interior of the first U-shaped member, a first rotating block is rotatably connected to the first pin, a second pin is fixedly connected to the interior of the second U-shaped member, a second rotating block is rotatably connected to the second pin, an electric push rod is fixedly connected to the bottom surface of the second rotating block, and the electric push rod is mounted on the strip.

[0007] Preferably, a servo motor is installed on the bottom surface of the mounting plate, and a rotating shaft is installed on the output end of the servo motor. The rotating shaft is rotatably connected to the inside of the mounting plate, and the rotating shaft is fixedly connected to the end of the strip away from the electric push rod. The top end of the rotating shaft is fixedly connected to the first rotating block.

[0008] Preferably, the bottom surface of the mounting plate is fixedly connected to three support rods, and the bottom end of each support rod is fixedly connected to a round pad.

[0009] Preferably, a sliding rod is fixedly connected to the inner wall of the mounting shell, and a sliding plate is slidably connected to the sliding rod.

[0010] Preferably, a spring is fixedly connected to the outer surface of the slide, and one end of the spring away from the slide is fixedly connected to the mounting shell.

[0011] Preferably, a placement plate is fixedly connected to the upper surface of the slide plate, and a group of placement cavities are opened inside the placement plate.

[0012] Beneficial effects:

[0013] Compared with the existing technology, the isothermal amplification kit has the following beneficial effects:

[0014] 1. The utility model realizes multi-dimensional and all-round shaking and mixing of reagent tubes in three-dimensional space by combining the rotational motion driven by a servo motor and the tilting action controlled by an electric push rod. This mixing method significantly improves the mixing efficiency and uniformity between reagents and samples, thereby effectively solving the problems of uneven mixing and low efficiency in traditional manual operation methods. Specifically, the servo motor drives the rotating shaft and the tilting mechanism connected to it to rotate, and at the same time, the electric push rod accurately controls the tilt angle of the mounting shell through the connection structure of the second rotating block and the second pin rod. The two work together to ensure that the sample and reagent in the reagent tube can be fully and evenly mixed.

[0015] Second, the utility model provides a spring and a slide rod, so that the micro-vibration generated during the shaking and mixing process further enhances the mixing effect, providing a more reliable guarantee for isothermal amplification experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional diagram of the utility model;

[0017] Figure 2 It is a three-dimensional diagram of the tilting mechanism of the utility model;

[0018] Figure 3 For this utility model Figure 2 A magnified schematic diagram of point A in the middle;

[0019] Figure 4It is a three-dimensional structural diagram of the slide plate, slide rod and spring of the utility model.

[0020] In the figure: 1. Mounting plate; 2. Servo motor; 3. Rotating shaft; 4. Tilt mechanism; 401. First U-shaped member; 402. First pin rod; 403. First rotating block; 404. Strip plate; 405. Electric push rod; 406. Second rotating block; 407. Second pin rod; 408. Second U-shaped member; 5. Mounting shell; 6. Placement plate; 7. Placement cavity; 8. Slide plate; 9. Sliding rod; 10. Spring; 11. Support rod. DETAILED DESCRIPTION

[0021] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0022] See also Figures 1 to 4 The isothermal amplification kit includes a mounting plate 1, and a mounting shell 5 is provided above the mounting plate 1. Three support rods 11 are fixedly connected to the bottom surface of the mounting plate 1, and a round pad is fixedly connected to the bottom end of each support rod 11 for stably supporting the entire kit.

[0023] A tilt mechanism 4 is installed between the mounting plate 1 and the mounting housing 5. This mechanism consists of multiple components, including a first U-shaped member 401 fixedly connected to the bottom surface of the mounting housing 5, a strip 404, and a second U-shaped member 408. A first pin 402 is fixed within the first U-shaped member 401, to which a first rotating block 403 is rotatably connected. A second pin 407 is fixed within the second U-shaped member 408, to which a second rotating block 406 is rotatably connected. A motorized push rod 405 is fixed to the bottom surface of the second rotating block 406, which is mounted on the strip 404.

[0024] A servo motor 2 is mounted on the bottom surface of the mounting plate 1, with a rotating shaft 3 attached to the output end of the servo motor 2. The rotating shaft 3 is rotatably connected to the interior of the mounting plate 1 and fixedly connected to the end of the strip 404 away from the electric push rod 405. The top end of the rotating shaft 3 is also fixedly connected to the first rotating block 403, forming a linked transmission system.

[0025] Two symmetrically arranged slide bars 9 are fixed to the inner wall of the mounting shell 5. A slide plate 8 is slidably connected to each slide bar 9. Four springs 10 are fixed to the outer surface of the slide plate 8, and the end of each spring 10 away from the slide plate 8 is fixedly connected to the mounting shell 5. This structure allows the slide plate 8 to slide stably on the slide bar 9 and provide elastic support through the springs 10. A placement plate 6 is fixed to the upper surface of the slide plate 8, and a group of placement cavities 7 are provided inside the placement plate 6 for placing reagent tubes.

[0026] When in use, by starting the servo motor 2 and the electric push rod 405, the mounting shell 5 and the slide 8, placement plate 6 and reagent tube inside it can be driven to perform multi-dimensional and all-round shaking and mixing, thereby achieving efficient and uniform mixing of the sample and reagent in the reagent tube.

[0027] The optimization of this isothermal amplification kit is primarily reflected in the design of the tilting mechanism 4 and spring 10. In the tilting mechanism 4, the connections between the first U-shaped member 401, the first pin 402, the first rotating block 403, the second U-shaped member 408, the second pin 407, and the second rotating block 406 are more precise, ensuring that the mounting housing 5, driven by the servo motor 2 and the electric push rod 405, can achieve more complex and efficient tilting motion.

[0028] At the same time, the material selection and manufacturing process of spring 10 have also been improved. Made of a material with greater elasticity and durability, spring 10 ensures stable elastic deformation during long-term use. This optimized spring 10 not only provides more stable support for slide 8 and placement plate 6, but also produces more significant elastic deformation during dynamic operation, further enhancing the mixing effect of the sample and reagent in the reagent tube.

[0029] Working Principle: When using this isothermal amplification kit, the operating steps are as follows:

[0030] First, the user needs to securely place the reagent tube into the placement cavity 7 on the placement plate 6. To ensure that the reagent tube does not shift or fall off during the subsequent mixing process, it needs to be firmly fixed with an external fixing cover. Then, the servo motor 2 is started. The servo motor 2 serves as a power source, and its output end drives the rotating shaft 3 to rotate. The rotating shaft 3 here is not just a simple transmission component. It is also closely connected to multiple key components in the tilting mechanism 4, such as the strip 404 and the first rotating block 403. Therefore, when the rotating shaft 3 rotates, it drives these components and the mounting shell 5 as a whole to rotate, thus forming the multi-dimensional motion required for mixing the reagent tubes. At the same time, the electric push rod 405 also starts working. The telescopic movement of the electric push rod 405 will precisely control the tilt angle of the mounting shell 5 in the vertical direction through the connection structure of the second rotating block 406 and the second pin 407. This tilting action, combined with the rotational motion driven by the servo motor 2, enables the mounting shell 5 to achieve complex and efficient tilting motion in multiple directions, thereby driving the reagent tubes placed in the placement cavity 7 to be fully and evenly shaken and mixed. It is worth noting that the four springs 10 also play an important role in the shaking and mixing process. They not only provide a stable supporting force for the slide 8 and the placement plate 6, but also can produce corresponding elastic deformation during the dynamic process. This deformation is further converted into micro-vibration, which is transmitted to the reagent tube through the slide 8 and the placement plate 6, thereby enhancing the mixing effect of the sample and reagent in the reagent tube. In summary, the isothermal amplification kit achieves efficient and uniform mixing of samples and reagents in the reagent tube. This automated and multi-dimensional mixing method not only improves the efficiency of the experiment, but also ensures the accuracy and reliability of the experimental results.

[0031] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An isothermal amplification kit, comprising a mounting plate (1), characterized in that: A mounting shell (5) is provided above the mounting plate (1), and a tilting mechanism (4) is provided between the mounting plate (1) and the mounting shell (5). The tilting mechanism (4) comprises a first U-shaped member (401) fixedly connected to the bottom surface of the mounting shell (5), a strip plate (404), and a second U-shaped member (408). The first U-shaped member (401) is fixedly connected to a first pin rod (402) inside, and a first rotating block (403) is rotatably connected to the first pin rod (402). The second U-shaped member (408) is fixedly connected to a second pin rod (407) inside, and a second rotating block (406) is rotatably connected to the second pin rod (407). The bottom surface of the second rotating block (406) is fixedly connected to an electric push rod (405), and the electric push rod (405) is mounted on the strip plate (404).

2. The isothermal amplification kit according to claim 1, wherein: A servo motor (2) is mounted on the bottom surface of the mounting plate (1), a rotating shaft (3) is mounted on the output end of the servo motor (2), the rotating shaft (3) is rotatably connected to the interior of the mounting plate (1), the rotating shaft (3) is fixedly connected to one end of the strip plate (404) away from the electric push rod (405), and the top end of the rotating shaft (3) is fixedly connected to the first rotating block (403).

3. The isothermal amplification kit according to claim 1, wherein: The bottom surface of the mounting plate (1) is fixedly connected to three support rods (11), and the bottom end of each support rod (11) is fixedly connected to a round pad.

4. The isothermal amplification kit according to claim 1, wherein: The inner wall of the installation shell (5) is fixedly connected with a slide bar (9), and the slide bar (9) is slidably connected with a slide plate (8).

5. The isothermal amplification kit according to claim 4, wherein: A spring (10) is fixedly connected to the outer surface of the slide plate (8), and one end of the spring (10) away from the slide plate (8) is fixedly connected to the mounting shell (5).

6. The isothermal amplification kit according to claim 4, wherein: The upper surface of the slide plate (8) is fixedly connected to a placement plate (6).

7. The isothermal amplification kit according to claim 6, wherein: A group of placement cavities (7) are provided inside the placement plate (6).

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