Piece dropping mechanism with cleaning function and cell slide preparation equipment

Abstract

The utility model discloses a piece dropping mechanism with a cleaning function and cell piece preparation equipment. The wafer dropping mechanism with the cleaning function comprises a supporting rack; the gantry bracket is assembled on the supporting rack; the liquid transferring assembly is connected with the gantry bracket, and the liquid transferring assembly can move relative to the supporting rack; the liquid adding assembly is assembled on the liquid transferring assembly and is used for releasing cleaning liquid; the liquid suction assembly is assembled on the gantry support and used for sucking cleaning waste liquid. According to the slide preparation device, the liquid transferring assembly, the liquid adding assembly and the liquid absorbing assembly are integrated on the gantry support, so that solution dropwise adding and slide cleaning in the slide preparation process can be carried out at the same station, the slide does not need to be moved, the risk that the slide falls off is effectively avoided, and the slide preparation efficiency and the success rate are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell preparation, in particular to a cell preparation device with cleaning function. BACKGROUND

[0002] In the existing cell preparation machine, the pipette and the cleaning device are generally in different stations, that is, the solution dropping and the slide cleaning during the preparation process need to be separated to different stations, which leads to the fact that the existing cell preparation machine generally needs to add a relatively complex true cavity suction cup and a moving mechanism to realize the movement of the slide, causing the problems of complex structure and high cost of the cell preparation machine, and the slide is moved back and forth for many times during the preparation process, which makes the slide prone to falling off, seriously affecting the preparation efficiency and success rate. CONTENT OF THE UTILITY MODEL

[0003] In order to overcome at least one of the defects of the prior art, the present application provides a cell preparation device with cleaning function, which can realize solution dropping and slide cleaning in the same station during the preparation process, effectively avoid the risk of slide falling off, and improve the preparation efficiency and success rate.

[0004] According to the cell preparation device with cleaning function, the liquid adding assembly is arranged on the moving assembly, and the liquid adding assembly is connected with the moving assembly, so that the liquid adding assembly can move relative to the support frame.

[0005] In the cell preparation device with cleaning function, the moving assembly, the liquid adding assembly and the liquid suction assembly are integrated on the gantry support, so that the solution dropping and the slide cleaning during the preparation process can be performed in the same station without moving the slide, effectively avoiding the risk of slide falling off, and improving the preparation efficiency and success rate.

[0006] According to some embodiments of the present application, the liquid adding assembly comprises a liquid adding support and a plurality of liquid adding units, the liquid adding support is connected with the moving assembly, and the plurality of liquid adding units are arranged on the liquid adding support along the length direction.

[0007] According to some embodiments of the present application, the liquid adding unit comprises a plurality of liquid adding needles, and the liquid outlet ends of the plurality of liquid adding needles are arranged in a diverging manner.

[0008] According to some embodiments of the present application, the liquid suction assembly comprises a liquid suction support and a plurality of liquid suction needles, the liquid suction support is connected with the gantry support, and the plurality of liquid suction needles are arranged on the liquid suction support in the length direction.

[0009] According to some embodiments of the present application, the liquid suction support is provided with a plurality of buffer cavities, a buffer block is movably connected in the buffer cavity, the liquid suction needle is fixedly connected with the buffer block, and an elastic member is abutted between the upper end of the buffer block and the upper end of the buffer cavity.

[0010] According to some embodiments of the present application, the liquid suction assembly comprises a liquid suction support and a plurality of liquid suction needles, the liquid suction support is connected with the gantry support, and the plurality of liquid suction needles are arranged on the liquid suction support in the length direction.

[0011] According to some embodiments of the present application, the liquid suction assembly comprises a liquid suction support and a plurality of liquid suction needles, the liquid suction support is connected with the gantry support, and the plurality of liquid suction needles are arranged on the liquid suction support in the length direction.

[0012] According to some embodiments of the present application, the liquid suction assembly comprises a liquid suction support and a plurality of liquid suction needles, the liquid suction support is connected with the gantry support, and the plurality of liquid suction needles are arranged on the liquid suction support in the length direction.

[0013] According to some embodiments of the present application, the liquid suction assembly comprises a liquid suction support and a plurality of liquid suction needles, the liquid suction support is connected with the gantry support, and the plurality of liquid suction needles are arranged on the liquid suction support in the length direction.

[0014] Based on the same inventive concept, the present application further provides a cell slice preparation device comprising the slice dripping mechanism with the cleaning function as described above.

[0015] In summary, the slice dripping mechanism with the cleaning function provided by the present application has the following technical effects:

[0016] By integrating the liquid suction assembly, the liquid adding assembly and the liquid suction assembly on the gantry support, the solution dripping and the slide cleaning in the slice preparation process can be performed at the same station without moving the slide, the risk of slide falling off is effectively avoided, and the slice preparation efficiency and success rate are improved. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 FIG. 1 is a structural schematic view of a cell slice preparation device according to an embodiment of the present application;

[0018] Figure 2 FIG. 4 is an enlarged schematic view of area A in FIG. 3; Figure 1

[0019] Figure 3 ​Structure schematic view of the drop piece mechanism with cleaning function of the embodiment of the present application;

[0020] Figure 4 Structure schematic view of the gantry support of the embodiment of the present application;

[0021] Figure 5 Front view of the gantry support of the embodiment of the present application;

[0022] Figure 6 Rear view of the gantry support of the embodiment of the present application.

[0023] In the drawings, the reference signs have the following meanings:

[0024] 1, support frame; 2, gantry support; 21, lifting driving member; 3, pipetting assembly; 31, pipetting support; 32, pipetting gun; 33, drying fan; 4, liquid adding assembly; 41, liquid adding support; 42, liquid adding unit; 421, liquid adding needle; 5, liquid sucking assembly; 51, liquid sucking support; 511, buffer cavity; 512, buffer block; 513, elastic member; 52, liquid sucking needle; 6, tool supporting plate. DETAILED DESCRIPTION

[0025] In order to better understand and implement, the technical solutions in the embodiments of the present application will be clearly and completely described below in combination with the drawings in the embodiments of the present application.

[0026] In the description of the present application, it should be noted that the terms “upper”, “lower”, “front”, “rear”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inner”, “outer” and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the purpose of facilitating the description of the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terms used in the specification of the present application are only for the purpose of describing the specific embodiments of the present application, and are not intended to limit the present application.

[0028] Reference is made to Figure 1 and Figure 3The application discloses a slide drop mechanism with a cleaning function. In some embodiments, the slide drop mechanism with the cleaning function comprises a support frame 1, a gantry support 2, a pipetting assembly 3, a liquid adding assembly 4 and a liquid sucking assembly 5. Preferably, the gantry support 2 is assembled on the support frame 1. The pipetting assembly 3 is connected with the gantry support 2 and can move relative to the support frame 1, and is used for dropping solution on a slide. The liquid adding assembly 4 is assembled on the pipetting assembly 3 and is used for releasing cleaning liquid. Optionally, the cleaning liquid comprises but is not limited to cleaning solution and water. The liquid sucking assembly 5 is assembled on the gantry support 2 and is used for sucking cleaning waste liquid. Preferably, the pipetting assembly 3, the liquid adding assembly 4 and the liquid sucking assembly 5 are integrated on the gantry support 2, so that solution dropping and slide cleaning in the slide preparation process can be performed at the same station without moving the slide, the risk of slide falling is effectively avoided, and the slide preparation efficiency and success rate are improved.

[0029] Optionally, when the pipetting assembly 3 moves relative to the support frame 1, the liquid adding assembly 4 can move along with the pipetting assembly 3. In this way, the liquid adding assembly 4 can perform initial cleaning on the slide to ensure that the surface of the slide is clean and free of impurities, or clean the residual fixing solution on the slide after cell fixation treatment to avoid affecting subsequent staining and observation, or clean the residual staining solution on the slide after cell staining and marking to help ensure the accuracy and clarity of the staining result, or perform final cleaning on the slide by the liquid adding assembly 4 after slide preparation to remove any reagent or impurity that may be left on the slide.

[0030] Referring to Figure 3 In some embodiments, a lifting driving member 21 is further included, the lifting driving member 21 is fixedly connected with the support frame 1, and the gantry support 2 is movably connected with the lifting driving member 21. Optionally, the lifting driving member 21 can be a motor, an air cylinder, an electric screw rod, an electric cylinder or the like. Preferably, tracks are fixedly connected to the two sides of the support frame 1, and sliding blocks are arranged on the two sides of the gantry support 2 and slide on the tracks. Further, the sliding blocks are driven by a motor through a connecting rod or directly to move on the sliding tracks. In this way, the gantry support 2 can drive the pipetting assembly 3, the liquid adding assembly 4 and the liquid sucking assembly 5 to lift, so that the pipetting assembly 3, the liquid adding assembly 4 and the liquid sucking assembly 5 can approach or move away from the slide.

[0031] Referring to Figure 4 , Figure 5 and Figure 6In some embodiments, the liquid transfer assembly 3 comprises a liquid transfer support 31 movably connected to the gantry support 2 and a liquid transfer gun 32 mounted on the liquid transfer support 31, and the liquid adding assembly 4 is mounted on the liquid transfer support 31. The liquid transfer gun 32 is used to mount a liquid transfer gun head to suck solution and drop the solution onto a slide. Optionally, the liquid transfer support 31 can be driven by a motor, a pneumatic cylinder, an electric screw, an electric cylinder or other driving devices to move the liquid transfer support 31 relative to the gantry support 2. Preferably, the gantry support 2 is provided with a linear guide motor arranged along the length direction to drive the liquid transfer support 31 to move along the length direction relative to the gantry support 2, thereby driving the liquid transfer gun 32 and the liquid adding assembly 4 to move along the length direction. Optionally, the liquid transfer gun 32 can be fixed on the liquid transfer support 31. Optionally, the liquid transfer gun 32 can be movably connected to the liquid transfer support 31. Optionally, the liquid transfer gun 32 can be driven by a multi-axis robot arm, a motor, a pneumatic cylinder, an electric screw, an electric cylinder or other driving devices to move relative to the liquid transfer support 31. Optionally, the liquid transfer gun 32 can be fixed on a rack driven by a motor mounted on the liquid transfer support 31 to move relative to the liquid transfer support 31.

[0032] In some embodiments, the tool base plate 6 is further provided with a plurality of placing grooves, the periphery of the placing grooves is provided with a liquid receiving groove, a slide is placed in the placing groove, the liquid adding assembly 4 is arranged opposite to the slide, and the liquid suction assembly 5 is arranged opposite to the liquid receiving groove. Optionally, the number of the slides is a plurality, the placing grooves correspond to the slides one by one, and the plurality of placing grooves are arranged along the length direction of the tool base plate 6. Preferably, the slide is placed in the placing groove, and the periphery of the placing groove is provided with the liquid receiving groove, so that the cleaning liquid of the cleaned slide flows into the liquid receiving groove for storage, effectively avoiding that the slide is soaked in the cleaning liquid for a long time. Further, the tool base plate 6 can rotate around its own axis to make the slide in an inclined state, effectively guiding the cleaning liquid on the slide to flow into the liquid receiving groove, and effectively avoiding water accumulation on the slide. In this embodiment, the liquid adding assembly 4 is arranged opposite to the slide, and the liquid suction assembly 5 is arranged opposite to the liquid receiving groove, so that when the gantry support 2 moves relative to the support rack 1 driven by the lifting drive 21, the gantry support 2 can drive the liquid transferring assembly 3 and the liquid suction assembly 5 to move towards the tool base plate 6, so that the liquid transferring assembly 3 is close to the slide, the liquid suction assembly 5 extends into the liquid receiving groove, the liquid adding assembly 4 releases the cleaning liquid to effectively flush the slide, improves the cleaning efficiency, and the liquid suction assembly 5 can suck away the cleaning liquid stored in the liquid receiving groove, thereby completing the cleaning of the slide and the cleaning liquid.

[0033] Referring to Figure 4 , Figure 5 and Figure 6 , in some embodiments, the liquid adding assembly 4 comprises a liquid adding support 41 and a plurality of liquid adding units 42, the liquid adding support 41 is connected with the liquid transferring assembly 3, and the plurality of liquid adding units 42 are arranged on the liquid adding support 41 along the length direction. Preferably, the liquid adding support 41 is fixedly connected with the liquid transferring support 31, so that when the liquid transferring support 31 moves relative to the gantry support 2 along the length direction, the liquid adding support 41 synchronously moves relative to the gantry support 2 along the length direction, so that the liquid adding units 42 arranged on the liquid adding support 41 can move along the length direction, realizing that the liquid adding assembly 4 moves with the liquid transferring assembly 3. In this embodiment, the number of the slides is greater than or equal to the number of the liquid adding units 42, so that the liquid adding units 42 can timely clean the slides that need to be cleaned by moving with the liquid transferring support 31, to ensure that the surface of the slide is clean and free of impurities when the liquid transferring assembly 3 drops the solution on the slide.

[0034] Referring toFigure 4 , Figure 5 and Figure 6 In some embodiments, the liquid adding unit 42 comprises a plurality of liquid adding needles 421, and the liquid outlet ends of the liquid adding needles 421 are arranged in a diverging manner. Preferably, the liquid outlet ends of the liquid adding needles 421 are arranged in a diverging manner, which effectively increases the cleaning area of each liquid adding unit 42, so that the cleaning area of the liquid adding unit 42 can cover the surface of the slide and sufficiently remove any reagent or impurity that may be left on the slide.

[0035] Referring to Figure 3 , Figure 4 , Figure 5 and Figure 6 In some embodiments, the liquid absorbing assembly 5 comprises a liquid absorbing support 51 connected to the gantry support 2 and a plurality of liquid absorbing needles 52 arranged on the liquid absorbing support 51 in the length direction. Optionally, the number of liquid absorbing needles 52 is greater than or equal to the number of slides, and the number of liquid absorbing needles 52 is greater than the number of liquid adding units 42. In this way, when the liquid absorbing assembly 5 absorbs the cleaning liquid, at least one liquid absorbing needle 52 is arranged in the liquid receiving groove of each placement groove to absorb and clean the cleaning liquid, preventing the cleaning liquid splashed to other placement grooves from remaining in the placement groove or the slide, so as to avoid affecting the subsequent processing and observation, and help to ensure the accuracy and clarity of the processing results.

[0036] Referring to Figure 2 In some embodiments, the liquid absorbing support 51 is provided with a plurality of buffer cavities 511, and a buffer block 512 is movably connected in the buffer cavity 511. The liquid absorbing needle 52 is fixedly connected to the buffer block 512, and an elastic member 513 is arranged between the upper end of the buffer block 512 and the upper end of the buffer cavity 511. Preferably, during the process in which the gantry support 2 drives the liquid absorbing assembly 5 to extend into the liquid receiving groove, when the distal end of the liquid absorbing needle 52 reaches the liquid receiving groove, the liquid absorbing needle 52 can drive the buffer block 512 to move upwardly in the buffer cavity 511, so that the elastic member 513 is compressed and deformed, thereby driving the liquid absorbing needle 52 to form an elastic contact with the liquid receiving groove, and preventing the liquid absorbing needle 52 from being damaged due to excessive force from the liquid receiving groove.

[0037] Referring to Figure 3 , Figure 4 and Figure 5In some embodiments, a drying fan 33 is further included, which is assembled to the pipette support 31. Preferably, the drying fan 33 moves along with the pipette support 31, so that the drying fan 33 is arranged opposite to the corresponding slide, and drives the drying fan 33 to effectively promote air circulation at the cleaned slide, accelerate the drying of the slide, and improve the efficiency of slide preparation. Specifically, the liquid adding needle 421 first releases cleaning liquid to the slide, and then the liquid sucking needle 52 sucks away the cleaning liquid for cleaning, and then the drying fan 33 is driven to promote air circulation at the cleaned slide, accelerate the drying of the slide, and improve the efficiency of slide preparation.

[0038] Referring to Figure 1 In some embodiments, a cell slide preparation device includes a slide cleaning mechanism as described above.

[0039] In some embodiments, the slides are placed one by one on the placement slots, and the gantry support 2 is driven to descend by the lifting drive 21, so that the gantry support 2 drives the pipette assembly 3, the liquid adding assembly 4, and the liquid sucking assembly 5 to descend, so that the pipette assembly 3, the liquid adding assembly 4, and the liquid sucking assembly 5 approach the slide. Specifically, when the gantry support 2 drives the pipette assembly 3 and the liquid sucking assembly 5 to descend toward the tool pallet 6, the pipette assembly 3 can be driven to approach the slide, and the liquid sucking assembly 5 can be driven to extend into the liquid receiving slot. Optionally, there is at least one liquid sucking needle 52 in the liquid receiving slot of each placement slot, and the number P of the slides and the number K of the liquid adding units 42 satisfy the relationship P = NK, where N is a positive integer. That is, the number of the slides is N times the number of the liquid adding units 42. Thus, when the slides are cleaned, the slides are divided into N groups, and the liquid adding needles 421 of the K liquid adding units 42 respectively release cleaning liquid to the K slides to clean the slides. Then, at least one liquid sucking needle 52 in the liquid receiving slot of each placement slot sucks the cleaning liquid for cleaning, so that the cleaning liquid splashed onto other placement slots is prevented from remaining on the placement slots or the slides, thereby avoiding affecting subsequent processing and observation, and helping to ensure the accuracy and clarity of the slide preparation results. At the same time, the drying fan 33 promotes air circulation at the cleaned slide, accelerates the drying of the slide, and improves the efficiency of slide preparation. After the current K slides are completely prepared, the pipette support 31 can be driven to move along the length direction relative to the gantry support 2 by a linear guide motor, so that the pipette gun 32 and the liquid adding assembly 4 move along the length direction, and the liquid adding needles 421 of the K liquid adding units 42 approach the next group of K slides. Thus, the liquid adding units 42 can timely clean the slides that need to be cleaned, so that the surface of the slide is clean and free of impurities when the pipette assembly 3 drops solution to the slide.

[0040] The technical means disclosed in the application scheme is not limited to the technical means disclosed in the above-mentioned embodiments, but also includes the technical solutions composed of any combination of the above technical features. It should be pointed out that for ordinary skilled in the art, without departing from the principles of the present application, a number of improvements and refinements can also be considered as the protection scope of the present application.

Claims

1. A dispensing mechanism with a cleaning function, characterized in that, It comprises: a support frame (1); a gantry frame (2) assembled on the support frame (1); a pipetting assembly (3) connected with the gantry frame (2) and movable relative to the support frame (1); a liquid adding assembly (4) assembled on the pipetting assembly (3) for releasing cleaning liquid; a liquid suction assembly (5) assembled on the gantry frame (2) for sucking cleaning waste liquid.

2. The drop sheet mechanism with cleaning function according to claim 1, characterized in that: The liquid adding assembly (4) comprises a liquid adding support (41) connected with the pipetting assembly (3) and a plurality of liquid adding units (42) arranged on the liquid adding support (41) in the length direction.

3. The drop sheet mechanism with cleaning function according to claim 2, characterized in that: The liquid adding unit (42) comprises a plurality of liquid adding needles (421), and the liquid outlet ends of the liquid adding needles (421) are arranged in a diverging manner.

4. The drop sheet mechanism with cleaning function according to claim 1, characterized in that: The liquid suction assembly (5) comprises a liquid suction support (51) connected with the gantry frame (2) and a plurality of liquid suction needles (52) arranged on the liquid suction support (51) in the length direction.

5. The drop sheet mechanism with cleaning function according to claim 4, characterized in that: The liquid suction support (51) is provided with a plurality of buffer cavities (511), a buffer block (512) is movably connected in the buffer cavity (511), the liquid suction needle (52) is fixedly connected with the buffer block (512), and an elastic member (513) is abutted between the upper end of the buffer block (512) and the upper end of the buffer cavity (511).

6. The drop sheet mechanism with cleaning function according to claim 1, wherein: The pipetting assembly (3) comprises a pipetting support (31) movably connected with the gantry frame (2) and a pipetting gun (32) assembled on the pipetting support (31), and the liquid adding assembly (4) is assembled on the pipetting support (31).

7. The drop sheet mechanism with cleaning function according to claim 6, characterized in that: It also comprises a drying fan (33) assembled on the pipetting support (31).

8. The drop sheet mechanism with cleaning function according to claim 1, characterized in that: It also comprises a lifting driving member (21) fixedly connected with the support frame (1), and the gantry frame (2) is movably connected with the lifting driving member (21).

9. The drop sheet mechanism with cleaning function according to claim 1, characterized in that: It also comprises a tool supporting plate (6) provided with a plurality of placing grooves, a liquid receiving groove is arranged on the periphery of each placing groove, a slide is placed in each placing groove, the liquid adding assembly (4) is arranged opposite to the slide, and the liquid suction assembly (5) is arranged opposite to the liquid receiving groove.

10. A cell preparation apparatus, characterized by comprising: It comprises the slide mechanism with cleaning function according to any one of claims 1-9. It comprises the slide mechanism with cleaning function according to any one of claims 1-9.

Images