Cooked tea fermentation strain detection device

Abstract

The utility model discloses a fermented tea fermentation strain detection device which comprises a main frame body, an auxiliary frame body, a sample seat, a sample groove, a high-throughput sequencer, a translation part, a lifting part and a sampling part, a sample seat and a high-throughput sequencer are fixedly connected in the main frame body; the sample groove is detachably connected to the sample seat; the auxiliary frame body is fixedly connected to the left side of the main frame body; one end of the translation part is detachably connected into the auxiliary frame body, and the other end of the translation part extends into the main frame body and is rotationally connected with the main frame body; the upper end of the lifting part is in threaded connection with the translation part, and the lower end is detachably connected with the sampling part. The automatic detection device has the advantages that the automatic detection process is effectively ensured, compared with manual operation, the automatic detection device is simple, and the working process is continuous and stable; and the detection efficiency is effectively improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of strain detection, particularly relates to a fermented tea fermentation strain detection device. BACKGROUND

[0002] The fermented tea fermentation strain detection device is a device for detecting and analyzing the composition and changes of microbial communities in the fermentation process of Pu'er fermented tea. At present, when detecting the fermentation strains of fermented tea, the fermented tea samples need to be manually extracted and then placed in a high-throughput sequencing device for Illumina MiSeq high-throughput sequencing technology. The DNA sequences of various microorganisms present in the Pu'er tea sample can be determined simultaneously, and the composition and abundance information of the microbial community can be obtained quickly and efficiently. The process of manually extracting and placing in a high-throughput sequencing device for strain detection is complicated, and the manual operation is discontinuous, which can easily lead to inaccurate measurement. SUMMARY

[0003] The technical problem to be solved by the utility model is to provide a fermented tea fermentation strain detection device.

[0004] To solve the above technical problem, the technical scheme of the utility model is as follows:

[0005] A fermented tea fermentation strain detection device, comprising a main frame, a secondary frame, a sample seat, a sample tank, a high-throughput sequencer, a translation part, a lifting part, and a sampling part. The sample seat and the high-throughput sequencer are fixedly connected in the main frame. The sample tank is detachably connected to the sample seat. The secondary frame is fixedly connected to the left side of the main frame. One end of the translation part is detachably connected to the secondary frame, and the other end extends into the main frame and is rotatably connected to the main frame. The lifting part is threadedly connected to the translation part at the upper end, and is detachably connected to the sampling part at the lower end.

[0006] Further, the controller is placed at the lower part of the secondary frame, and is electrically connected to the translation part, the lifting part, and the sampling part.

[0007] Further, the translation part comprises a motor and a lead screw. The motor is detachably connected to the upper part of the secondary frame, and its output end is fixedly connected to one end of the lead screw, and the other end extends into the main frame and is rotatably connected to the main frame.

[0008] Further, the lifting part comprises a moving seat and an electric cylinder. The moving seat is threadedly connected to the translation part. The lower end of the moving seat is detachably connected to the upper end of the electric cylinder.

[0009] Further, the sampling part comprises a connecting plate, a plurality of sampling pumps detachably connected to the connecting plate, and a sampling tube in communication with the sampling pumps.

[0010] Further, a sliding groove is arranged in the main frame and above the translation part; a T-shaped sliding block is fixed to the upper end of the lifting part; the T-shaped sliding block is arranged in the sliding groove and can move relative to the sliding groove.

[0011] The present application has the following beneficial effects:

[0012] The electric cylinder in the lifting part is controlled to stretch, so that the sampling part at the lower end of the lifting part samples the sample in the sample groove. BRIEF DESCRIPTION OF DRAWINGS

[0013] Fig. 1 The present application has the following beneficial effects:

[0014] Fig. 2 The present application has the following beneficial effects:

[0015] In the drawings,

[0016] 1-main frame, 2-sub frame, 3-sample seat, 4-sample groove, 5-high-throughput sequencer, 6-translation part, 7-lifting part, 8-sampling part, 9-controller, 10-sliding groove, 11-T-shaped sliding block;

[0017] 61-motor, 62-screw rod; 71-moving seat, 72-electric cylinder, 81-sampling pump, 82-sampling tube, 83-connection plate. DETAILED DESCRIPTION

[0018] The specific embodiments of the present application will be further described below in conjunction with the drawings. It should be noted that the description of these embodiments is used to help understand the present application and does not constitute a limitation of the present application. In addition, the technical features involved in each embodiment of the present application described below can be combined with each other as long as they do not conflict with each other.

[0019] A fermented tea fermentation strain detection device, referring to Figs. 1-2As shown, including the main frame 1, the secondary frame 2, the sample seat 3, the sample tank 4, the high-throughput sequencer 5, the translation part 6, the lifting part 7, the sampling part 8; The main frame 1 is fixedly connected with the sample seat 3 and the high-throughput sequencer 5; The sample tank 4 is detachably connected to the sample seat 3; The secondary frame 2 is fixedly connected to the left side of the main frame 1; One end of the translation part 6 is detachably connected in the secondary frame 2, the other end extends into the main frame 1 and is rotatably connected with the main frame 1; The upper end of the lifting part 7 is threadedly connected with the translation part 6, and the lower end is detachably connected with the sampling part 8.

[0020] It should be noted that the main frame 1 is the main part of the test; The secondary frame 2 is used for fixing the motor 61 and the rest of the parts; The sample seat 3 is used for placing the sample tank 4; Different samples can be loaded into the sample tank 4; The high-throughput sequencer 5 can simultaneously determine the DNA sequence of various microorganisms existing in the Pu'er tea sample, quickly and efficiently obtain the strain community composition and abundance information, for the prior art, the person skilled in the art can select and use according to the specific circumstances, and the details are not repeated; The translation part 6 is used for moving the lifting part 7; The lifting part 7 is used for lifting the sampling part 8; The sampling part 8 is used for sampling the sample in the sample tank 4.

[0021] Specifically, the lower part of the secondary frame 2 is provided with a controller 9; The controller 9 is electrically connected with the translation part 6, the lifting part 7 and the sampling part 8 respectively. The controller 9 is prior art, which can control the actions of the translation part 6, the lifting part 7 and the sampling part 8.

[0022] Specifically, the translation part 6 includes a motor 61 and a lead screw 62; The motor 61 is detachably connected to the upper part of the secondary frame 2, the output end thereof is fixedly connected with one end of the lead screw 62, and the other end extends into the main frame 1 and is rotatably connected with the main frame 1. The motor 61 can drive the lead screw 62 to rotate, so that the lifting part 7 can move and sample between the sample tank 4 and the high-throughput sequencer 5;

[0023] Specifically, the lifting part 7 includes a moving seat 71 and an electric cylinder 72; The moving seat 71 is threadedly connected with the translation part 6; The lower end of the moving seat 71 is detachably connected with the upper end of the electric cylinder 72. The moving seat 71 is used for threadedly connecting with the lead screw 62, which is convenient for translation; The electric cylinder 72 is used for lifting the sampling part 8.

[0024] Specifically, the sampling part 8 includes a connecting plate 83, a plurality of sampling pumps 81 detachably connected to the connecting plate 83 and a sampling tube 82 in communication with the sampling pump 81. The sampling pump 81 and the sampling tube 82 cooperate to sample the sample in the sample tank 4.

[0025] Specifically, in order to ensure that the lifting part 7 moves reciprocatingly and stably in one direction on the translation part 6, a sliding groove 10 is arranged in the main frame 1 and above the translation part 6; A T-shaped sliding block 11 is fixedly connected to the upper end of the lifting part 7; The T-shaped sliding block 11 is arranged in the sliding groove 10 and can move relative to the sliding groove 10.

[0026] The working principle of the utility model:

[0027] When the sample is detected, the controller 9 controls the translation part 6 to move, so that the lifting part 7 is just above the sample groove 4, then the electric cylinder 72 in the lifting part 7 is stretched, so that the sampling part 8 at the lower end of the lifting part 7 samples the sample in the sample groove 4, after sampling, the electric cylinder 72 in the lifting part 7 is retracted, the translation part 6 moves to the high-throughput sequencer 5, after reaching the high-throughput sequencer 5, the electric cylinder 72 in the lifting part 7 is stretched, so that the sampling part 8 at the lower end of the lifting part 7 discharges the sample in the sampling tube 82 to the predetermined detection position on the high-throughput sequencer 5 through the sampling pump 81 to detect the tea fermentation bacteria, thus completing a working process, effectively ensuring the automatic detection process, compared with manual operation, the working process is simple and stable; the detection efficiency is improved.

[0028] The embodiments of the utility model are described in detail in combination with the drawings, but the utility model is not limited to the described embodiments. For those skilled in the art, various changes, modifications, replacements and modifications can be made to these embodiments without departing from the principles and spirits of the utility model, and still fall within the protection scope of the utility model.

Claims

1. A fermented tea fermentation strain detection device, characterized by: The utility model relates to a high throughput sequencer, including main frame (1), vice frame (2), sample seat (3), sample groove (4), high throughput sequencer (5), translation part (6), lifting part (7), sampling part (8), the main frame (1) in fixedly connected with sample seat (3) and high throughput sequencer (5), the sample groove (4) is detachably connected on sample seat (3), vice frame (2) is fixedly connected to the left side of main frame (1), one end of translation part (6) is detachably connected in vice frame (2), and the other end extends into main frame (1) and is rotatably connected with main frame (1), the upper end of lifting part (7) is threadedly connected with translation part (6), and the lower end is detachably connected sampling part (8).

2. The fermented tea fermentation bacteria detection device according to claim 1, characterized in that: The controller (9) is placed in the lower part of the vice frame (2), and the controller (9) is electrically connected with the translation part (6), the lifting part (7) and the sampling part (8) respectively.

3. The fermented tea fermentation bacteria detection device according to claim 2, characterized in that: The translation part (6) includes a motor (61) and a screw rod (62), the motor (61) is detachably connected to the upper part of the vice frame (2), the output end of the motor (61) is fixedly connected with one end of the screw rod (62), and the other end of the screw rod (62) extends into the main frame (1) and is rotatably connected with the main frame (1).

4. The fermented tea fermentation bacteria detection device according to claim 1, characterized in that: The lifting part (7) includes a moving seat (71) and an electric cylinder (72), the moving seat (71) is threadedly connected with the translation part (6), and the lower end of the moving seat (71) is detachably connected with the upper end of the electric cylinder (72).

5. The fermented tea fermentation bacteria detection device according to claim 1, characterized in that: The sampling part (8) includes a connecting plate (83), a plurality of sampling pumps (81) detachably connected to the connecting plate (83) and a sampling tube (82) communicated with the sampling pumps (81).

6. The apparatus for detecting a fermentation bacteria species of a fermented tea according to any one of claims 1 to 5, characterized in that: The main frame (1) is provided with a sliding groove (10) above the translation part (6), the upper end of the lifting part (7) is fixedly connected with a T-shaped sliding block (11), and the T-shaped sliding block (11) is arranged in the sliding groove (10) and can move relative to the sliding groove (10).

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