Preliminary enrichment device for vibrio bacteriophage

Abstract

The utility model relates to the technical field of bacteriophage fermentation devices, in particular to a preliminary enrichment device for vibrio bacteriophages, which comprises a sample adding bin, convex lenses and a bin cover, a plurality of hole channels are arranged at the top of the sample adding bin in a matrix manner, and one convex lens is embedded at the lower end of each hole channel; the side portion of the convex lens is connected with the hole wall of the hole channel in a sealed mode. The bin cover is buckled at the top of the sample adding bin, and the bottom of the bin cover is hermetically connected with the top of the hole channel; the utility model provides a preliminary enrichment device for vibrio bacteriophages, which is convenient for treating a plurality of vibrio bacteriophage water samples at one time and improving the treatment efficiency by arranging a plurality of hole channels on a sample adding bin, and is convenient for amplifying the water samples in the hole channels and observing the water samples by arranging convex lenses at the lower ends of the hole channels.

Description

TECHNICAL FIELD

[0001] The utility model relates to bacteriophage fermentation device technical field especially relates to a preliminary enrichment device of vibrio bacteriophage. BACKGROUND

[0002] In marine and freshwater environments, the study of vibrio and its bacteriophage is of great significance for understanding microbial ecology and developing new antibacterial strategies. The separation and enrichment of vibrio bacteriophage from complex water samples usually requires a series of cumbersome steps, including pre-enrichment, centrifugation, filtration, etc.

[0003] In the prior art, some devices for bacteriophage enrichment have been disclosed, for example, the patent with publication number CN212877542U introduces a bacteriophage enrichment device, which mainly includes a protective cover, an enrichment cylinder, an upper support membrane, a filtrate net, a lower support membrane, microsphere filler, a conduit, a feed hopper, a support pedestal, a stable base and a mechanical valve.

[0004] As in the above technical solution, the enrichment cylinder is a single cylinder structure, which can only process one water sample at a time. Therefore, the existing device has low processing efficiency when dealing with a large number of vibrio bacteriophage water samples. To solve the above problems, it is necessary to provide a preliminary enrichment device of vibrio bacteriophage. UTILITY MODEL CONTENT

[0005] Therefore, the utility model provides a preliminary enrichment device of vibrio bacteriophage, which is convenient for processing several vibrio bacteriophage water samples at a time by setting several hole channels on the sample adding bin, thereby improving the processing efficiency.

[0006] The technical scheme of the utility model is as follows:

[0007] The utility model provides a preliminary enrichment device of vibrio bacteriophage, which includes a sample adding bin, and further includes a convex lens and a bin cover, wherein,

[0008] The top of the sample adding bin is provided with several hole channels, and the lower end of each hole channel is inlaid with a convex lens;

[0009] The side of the convex lens is sealingly connected with the hole wall of the hole channel;

[0010] The bin cover is buckled on the top of the sample adding bin, and the bottom of the bin cover is sealingly connected with the top of the hole channel.

[0011] On the basis of the above technical scheme, preferably, the side of the sample adding bin is sequentially provided from bottom to top with 5mL scale line and 10mL scale line.

[0012] On the basis of the above technical solutions, preferably, the side of the cover is hingedly connected with a buckling part, and the side of the sample adding chamber is provided with an outer edge, wherein,

[0013] One end of the buckling part is clamped and connected with the bottom of the outer edge.

[0014] On the basis of the above technical solutions, preferably, one end of the buckling part is provided with a buckling tooth, and the buckling tooth abuts against the bottom of the outer edge.

[0015] On the basis of the above technical solutions, preferably, the bottom of the cover is provided with a sealing protrusion at a position corresponding to the hole.

[0016] The sealing protrusion is inlaid in the inner side of the hole.

[0017] On the basis of the above technical solutions, preferably, the sealing protrusion is an inverted circular truncated cone.

[0018] On the basis of the above technical solutions, preferably, the culture medium is further included, wherein,

[0019] The culture medium is provided with at least one bag in each hole.

[0020] On the basis of the above technical solutions, preferably, the anti-skid pad is further included, wherein,

[0021] The anti-skid pad is fixed on the bottom of the sample adding chamber, and the anti-skid pad is provided with an observation hole at a position corresponding to the hole.

[0022] On the basis of the above technical solutions, preferably, one side of the sample adding chamber is provided with a hot shoe slot, and the other side is provided with a T-shaped insertion block.

[0023] On the basis of the above technical solutions, preferably, a plurality of turbulence protrusions are arranged on the hole wall of the hole.

[0024] The preliminary enrichment device for vibrio bacteriophage has the following beneficial effects compared with the prior art:

[0025] (1) By arranging a plurality of holes on the sample adding chamber, a plurality of vibrio bacteriophage water samples can be processed at a time, and the processing efficiency is improved. At the same time, by arranging a convex lens at the lower end of the hole, the water sample in the hole can be enlarged, and the water sample can be observed conveniently.

[0026] (2) By arranging the sealing protrusion, the air tightness of the hole is improved, and the fermentation effect is improved.

[0027] (3) By arranging the culture medium, the salinity of the water body is improved, so that non-salt-loving microorganisms in the water body cannot grow, and the problem that some non-salt-loving bacteria compete for nutrients during vibrio fermentation is solved.

[0028] (4) By setting the anti-skid pad, prevent skid when shaking, at the same time, by setting the spoiler protrusion, facilitate the disturbance of water sample, improve the shock effect.

[0029] (5) By setting the hot shoe slot and T-shaped block, facilitate the splicing of two or more than two sample adding bins, so that the vibrio bacteriophage water sample treated at one time can be more. BRIEF DESCRIPTION OF DRAWINGS

[0030] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiment or the prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of these drawings.

[0031] Figure 1 It is a perspective view of a vibrio bacteriophage preliminary enrichment device of the present application;

[0032] Figure 2 It is another perspective view of a vibrio bacteriophage preliminary enrichment device of the present application;

[0033] Figure 3 It is a bottom view of a vibrio bacteriophage preliminary enrichment device of the present application;

[0034] Figure 4 It is an A-A sectional view of the present application;

[0035] Figure 5 It is a perspective view of a sample adding bin of the present application;

[0036] Figure 6 It is a perspective view of a bin cover of the present application;

[0037] Figure 7 It is a splicing structure schematic view of two sample adding bins of the present application;

[0038] In the figure: 1, sample adding bin; 2, convex lens; 3, bin cover; 4, culture medium; 5, anti-skid pad; 101, hole; 102, outer edge; 103, hot shoe slot; 104, T-shaped block; 105, spoiler protrusion; 301, buckling part; 302, buckling tooth; 303, sealing protrusion; 501, observation hole. DETAILED DESCRIPTION

[0039] The technical solutions in the utility model will be clearly and completely described below in combination with the specific implementation manners of the utility model. Apparently, the described implementation manners are only a part of the implementation manners of the utility model, rather than all the implementation manners. Based on the implementation manners in the utility model, all the other implementation manners obtained by the ordinary skilled in the art without making creative efforts belong to the protection scope of the utility model.

[0040] As shown in the utility model discloses a vibrio phage preliminary enrichment device, including adding sample bin 1, convex lens 2 and bin cover 3. Figures 1-7

[0041] Among them, adding sample bin 1 is rectangular block, material is PP, and its top matrix is provided with a plurality of circular holes 101, Figure 5 Among them, hole 101 matrix sets up sixteen, convex lens 2 is used to block hole 101, and is used to enlarge water sample in hole 101, to facilitate observation water sample, specifically, the lower end of each hole 101 is inlaid with a convex lens 2, and the side of convex lens 2 is sealedly connected with the hole wall of hole 101, as shown in the utility model discloses a vibrio phage preliminary enrichment device, including adding sample bin 1, convex lens 2 and bin cover 3. Figure 4 As shown, the top edge of convex lens 2 and the hole wall of hole 101 are arc-shapedly connected and transitioned, the bottom surface of convex lens 2 is aligned with the bottom surface of adding sample bin 1, that is, the top surface of convex lens 2 is arc-shaped, and the bottom surface is flat, and when observing from the plane to hole 101, the enlarged water sample can be observed.

[0042] As shown in the utility model discloses a vibrio phage preliminary enrichment device, including adding sample bin 1, convex lens 2 and bin cover 3. Figure 4 As shown, after hole 101 is blocked by the convex lens 2 at the lower end, an inside of hole 101 forms a sample adding hole, specifically, the sample adding hole has a diameter of 1.5 cm, the top end surface of adding sample bin 1 is square-shaped, and the side length is 9 cm; 10 mL of sample can be treated in each hole, and the sample has the following characteristics: water body salinity is 0.10-0.25%.

[0043] For convenient metering, as shown in the utility model discloses a vibrio phage preliminary enrichment device, including adding sample bin 1, convex lens 2 and bin cover 3. Figure 1 As shown, the side of adding sample bin 1 is sequentially provided with 5mL scale line and 10mL scale line from bottom to top, specifically, the sample adding hole has a height of 10 cm, the 10mL scale line is arranged at a position about 5.7 cm high from the bottom of adding sample bin 1 upwards, and the 5mL scale line is arranged at a position about 5.7 cm high from the bottom of adding sample bin 1 upwards; the total volume of adding sample bin 1 is 17.7 mL.

[0044] Bin cover 3 is used for blocking hole 101, and the material is PP, as shown in the utility model discloses a vibrio phage preliminary enrichment device, including adding sample bin 1, convex lens 2 and bin cover 3. Figure 1 As shown, bin cover 3 is square-shaped with a size of 9cm*9cm, and is buckled on the top of adding sample bin 1 through a safety buckle, and the bottom of bin cover 3 is sealingly connected with the top of hole 101. Specifically, as shown in the utility model discloses a vibrio phage preliminary enrichment device, including adding sample bin 1, convex lens 2 and bin cover 3. Figure 4 ​As shown, the side of the cover 3 is hinged with a plate-shaped fastening part 301, and the side of the sample adding tank 1 is provided with an outer edge 102, wherein one end of the fastening part 301 is in snap-fit connection with the bottom of the outer edge 102, and further, one end of the fastening part 301 is provided with a fastening tooth 302, which is plate-shaped with a size of 0.2 cm*0.4 cm*3.5 cm, and after fastening, the fastening tooth 302 is in abutment against the bottom of the outer edge 102.

[0045] A flexible and flexible deformation bending part is arranged between the cover 3 and the fastening part 301, and the cover 3 and the fastening part 301 are hinged through the bending part. When the cover 3 is fastened, the fastening part 301 is turned down to move the fastening tooth 302 to the bottom of the outer edge 102, and with a little force, the fastening tooth 302 is clamped into the bottom of the outer edge 102 to form a snap-fit relationship, and the fastening and fixing of the cover 3 and the sample adding tank 1 are completed. Figure 1 In the structure, the outer edge 102 is a square flange-shaped structure, and one fastening part 301 is arranged at each side of the sample adding tank 1. When the cover 3 is fastened, the four fastening parts 301 are respectively fastened on the outer edges 102 at the corresponding positions. This structure facilitates the installation and removal of the cover 3.

[0046] In addition, the bottom of the cover 3 and the position corresponding to the channel 101 are provided with a sealing protrusion 303, wherein the sealing protrusion 303 is a rubber plug, which is in the shape of an inverted circular truncated cone or a polytype of a truncated circular cone and a cylinder. When the sealing protrusion 303 is a polytype of a truncated circular cone and a cylinder, the large circular diameter of the truncated circular cone is 1.7 cm, the small circular diameter is 1.4 cm, and the height is 0.5 cm. The large circular diameter of the cylinder is 1.7 cm, and the height is 0.5 cm. After the cover 3 is installed, the lower end or the whole of the sealing protrusion 303 is embedded in the inner side of the channel 101, which is used to seal the channel 101 and improve the air tightness, thereby improving the fermentation effect.

[0047] In addition, when vibrio is fermented, some non-halophilic bacteria are easy to snatch nutrients. Therefore, at least one bag of culture medium 4 is arranged in each channel 101, wherein the culture medium 4 is a bagged self-dissolving base body, which is composed of sodium chloride 0.15 g, glucose 0.2 g, and trypsin 0.2 g. The culture medium 4 is used to improve the salinity of the water body, so that the non-halophilic microorganisms in the water body cannot grow, and an environment suitable for the growth of vibrio host is created, which also provides an effective, simple and innovative idea for the preliminary enrichment of vibrio bacteriophages in the future.

[0048] After the sample and the culture solution are injected into the sample adding hole, it is necessary to perform shock treatment. In order to prevent slipping, an anti-skid pad 5 is fixedly arranged at the bottom of the sample adding tank 1, and the anti-skid pad 5 is used to prevent slipping.

[0049] In addition, as shown in the drawings, Figure 4 In order to facilitate the observation of the water sample in the sample adding hole, an observation hole 501 is arranged on the anti-skid pad 5 and at the position corresponding to the channel 101.

[0050] In addition, as Figure 4 shown, a number of turbulence protrusions 105 are arranged on the hole wall of the hole 101, and in the oscillation process, the water body collides with the turbulence protrusions 105 to form turbulence, which can effectively improve the oscillation effect and accelerate the mixing of the water sample and the dissolved substances of the culture medium 4.

[0051] In actual use, in order to dispose of more water samples at one time, a hot shoe slot 103 is arranged on one side of the sample adding bin 1, and a T-shaped plug 104 is arranged on the other side, as Figure 7 shown, the hot shoe slot 103 and the T-shaped plug 104 can be inserted and matched, and the insertion of the hot shoe slot 103 and the T-shaped plug 104 can splice several sample adding bins 1 together, Figure 7 two sample adding bins 1 are spliced, and after splicing, more water samples can be disposed of at one time.

[0052] The use method of the vibrio bacteriophage preliminary enrichment device includes the following steps:

[0053] 1. Open the bin cover 3, centrifuge the water sample to be measured at 5000 rpm for 10 min, take 10 mL of supernatant sample into the hole 101 of the sample adding bin 1, add 100 microliters of host bacteria liquid (OD600=0.5) into the hole 101, add the bin cover 3, and buckle the buckle teeth 302;

[0054] 2. After 16 samples are added, oscillate the vibrio bacteriophage enrichment device up and down for 1 min to make the culture medium 4 completely dissolved, and in the process, the change of the water sample can be observed through the convex lens 2, and the oscillation can be manual or in an oscillation incubator;

[0055] 3. Place the vibrio bacteriophage enrichment device in a 37℃ environment for overnight culture or in a 37℃, 200 rpm oscillation incubator for 6 h, which completes the preliminary enrichment step of the bacteriophage.

[0056] The above is only a preferred embodiment of the present application, and is not used to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.

Claims

1. A preliminary enrichment device for Vibrio phages, comprising a sample loading chamber (1), characterized in that: It also includes a convex lens (2) and a bin cover (3), wherein, The top matrix of the sample loading chamber (1) is provided with a number of channels (101), and a convex lens (2) is embedded at the lower end of each channel (101); The side of the convex lens (2) is sealed to the wall of the channel (101); The cover (3) is fastened to the top of the sample loading chamber (1), and the bottom of the cover (3) is sealed to the top of the channel (101).

2. The vibrio phage preliminary enrichment device as described in claim 1, characterized in that: The side of the sample loading chamber (1) is provided with a 5mL scale line and a 10mL scale line from bottom to top.

3. The vibrio-like bacteriophage preliminary enrichment device as described in claim 1, characterized in that: The side of the cover (3) is hinged with a fastening part (301), and the side of the sample loading chamber (1) is provided with an outer edge (102), wherein, One end of the fastening part (301) is engaged with the bottom of the outer edge (102).

4. The vibrio-like bacteriophage preliminary enrichment device as described in claim 3, characterized in that: One end of the fastening part (301) is provided with a fastening tooth (302), which abuts against the bottom of the outer edge (102).

5. The vibrio-like bacteriophage preliminary enrichment device as described in claim 1, characterized in that: A sealing protrusion (303) is provided at the bottom of the cover (3) and at the position corresponding to the hole (101), wherein, The sealing protrusion (303) is embedded inside the channel (101).

6. The vibrio-like bacteriophage preliminary enrichment device as described in claim 5, characterized in that: The sealing protrusion (303) is an inverted frustum shape.

7. The vibrio-like bacteriophage preliminary enrichment device as described in claim 1, characterized in that: It also includes culture medium (4), wherein, The culture medium (4) is provided in at least one bag in each of the channels (101).

8. The device for preliminary enrichment of Vibrio phages as described in claim 1, characterized in that: It also includes an anti-slip mat (5), in which, The anti-slip pad (5) is fixed to the bottom of the sample loading chamber (1), and an observation hole (501) is provided on the anti-slip pad (5) at the position corresponding to the channel (101).

9. The device for preliminary enrichment of Vibrio phages as described in claim 1, characterized in that: The sample loading chamber (1) is provided with a hot shoe slot (103) on one side and a T-shaped insert (104) on the other side.

10. The device for preliminary enrichment of Vibrio phages as described in claim 1, characterized in that: The wall of the channel (101) is provided with a few turbulence protrusions (105).

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