Culture bottle convenient for changing liquid

Abstract

The utility model relates to the field of culture bottles, in particular to a culture bottle convenient to change liquid, which comprises a culture bottle body, a first bottle opening and a second bottle opening, a first bottle cap is mounted on the first bottle opening, a second bottle cap is mounted on the second bottle opening, and the culture bottle body, the first bottle opening, the first bottle cap, the second bottle opening and the second bottle cap form a closed cavity. According to the cell culture bottle, when liquid is changed in the cell culture process, cells can be intercepted in the culture bottle through the filter membrane, so that the cultured cells are separated from culture liquid to be discharged, the cells do not need to be separated in a centrifugal mode or other modes, and the workload of changing the liquid is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to a culture bottle technical field especially, it is convenient to change liquid culture bottle. BACKGROUND

[0002] In recent years, with the rapid development of cell therapy industry, as an important link in the production process of cell therapy drugs, the demand for cell culture is also increasing.

[0003] At present, when using most of the cell culture bottles for cell culture, the liquid needs to be changed several times in the middle, and during the liquid changing process, the culture medium in the original cell culture solution needs to be separated and then new culture medium is added, and most of the current separation formula is centrifugal separation, which is troublesome and low in efficiency. UTILITY MODEL CONTENT

[0004] The utility model aims at solving the shortcomings in the prior art and provides a convenient liquid changing culture bottle.

[0005] In order to achieve the above object, the utility model adopts the following technical scheme:

[0006] A convenient liquid changing culture bottle, including culture bottle body, first bottle mouth and second bottle mouth, the first bottle mouth is installed with first bottle cover, the second bottle mouth is installed with second bottle cover, the culture bottle body and the first bottle mouth, the first bottle cover, the second bottle mouth, the second bottle cover form a closed cavity, and the first bottle mouth is internally provided with filter membrane.

[0007] Preferably, the filter membrane adopts a material through which the culture medium can pass but the cells cannot.

[0008] Preferably, the culture bottle body is in the shape of a boat, and the first bottle mouth and the second bottle mouth on both sides of the culture bottle body are inclined upwards and outwards.

[0009] Preferably, the bottom of the culture bottle body contains cell culture solution.

[0010] Preferably, the first bottle cover is provided with a first breathing membrane, and the second bottle cover is provided with a second breathing membrane.

[0011] Preferably, the first breathing membrane and the second breathing membrane adopt a material through which gas can pass but microorganisms cannot.

[0012] The utility model has the advantages of:

[0013] In the utility model, when the liquid is changed during the cell culture process, the cells can be retained in the culture bottle through the filter membrane, so as to separate the cultured cells from the culture solution to be discharged, so that the cells do not need to be separated by centrifugation or other methods, and the workload of liquid changing is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 The utility model provides a convenient liquid change culture bottle's structure schematic diagram.

[0015] In the drawing: 1, first bottle cover, 2, first breath membrane, 3, first bottle mouth, 4, filter membrane, 5, culture bottle body, 6, cell culture solution, 7, second bottle mouth, 8, second bottle cover, 9, second breath membrane. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments.

[0017] Referring to Figure 1 A kind of convenient liquid change culture bottle, including culture bottle body 5 and first bottle mouth 3, second bottle mouth 7, first bottle cover 1 is installed on first bottle mouth 3, second bottle cover 8 is installed on second bottle mouth 7, culture bottle body 5 and first bottle mouth 3, first bottle cover 1, second bottle mouth 7, second bottle cover 8 form a closed cavity, culture bottle body 5 bottom can be used to hold cell culture solution 6 and carry out cell culture;

[0018] First bottle cover 1 is provided with first breath membrane 2, and second bottle cover 8 is provided with second breath membrane 9, for gas exchange when cell culture, provide the required gas for the cell cultivated in culture bottle.

[0019] First bottle mouth 3 is provided with filter membrane 4 inside, for when needing to change liquid, cell is filtered and retained in culture bottle interior.

[0020] Among them, filter membrane 4 uses the membrane material with pore size that can filter cell, and can be directly purchased in market.

[0021] According to the liquid transfer process of the culture bottle of the technical scheme as follows:

[0022] Before cell culture, as shown in Figure 1 Second bottle cover 8 on second bottle mouth 7 of culture bottle can be opened, and the cell to be cultivated and other reagents are added, then second bottle cover 8 is covered, and the culture bottle is placed in the incubator for cultivation. When the cell culture reaches a certain time, liquid change is needed, the culture bottle is taken out from the incubator, first bottle cover 1 on first bottle mouth 3 is opened, and cell culture solution 6 is poured out from first bottle mouth 3. In the pouring process, the cells in the cell culture solution 6 are intercepted in the culture bottle body 5 due to the presence of filter membrane 4, and the culture medium in the culture solution is discharged from the first bottle mouth 3. Then, new culture medium can be added to the culture bottle body 5 from the first bottle mouth 3 or the second bottle mouth 7, and a liquid change operation is completed.

[0023] In the utility model, when liquid is changed in the cell culture process, the cells can be intercepted in the culture bottle body 5 through the filter membrane 4, so that the cultured cells are separated from the culture liquid to be discharged, the cells do not need to be separated through centrifugation or other ways, and the workload of liquid changing is reduced.

[0024] The above is only the preferred specific implementation of the utility model, but the protection scope of the utility model is not limited to this, any skilled person in the art can make equivalent replacement or change according to the technical scheme and the utility model concept of the utility model within the technical range disclosed by the utility model, which should be covered in the protection scope of the utility model.

Claims

1. A convenient medium changing culture bottle comprising a culture bottle body (5), a first bottle mouth (3) and a second bottle mouth (7), characterized in that, The first bottle mouth (3) is provided with a first bottle cap (1), the second bottle mouth (7) is provided with a second bottle cap (8), the culture bottle body (5) and the first bottle mouth (3), the first bottle cap (1), the second bottle mouth (7) and the second bottle cap (8) form a closed cavity, and the first bottle mouth (3) is internally provided with a filter membrane (4).

2. The culture bottle according to claim 1, wherein The culture bottle body (5) is in the shape of a boat, and the first bottle mouth (3) and the second bottle mouth (7) on both sides of the culture bottle body (5) are both inclined upwards and outwards.

3. The culture bottle according to claim 2, wherein The bottom of the culture bottle body (5) contains cell culture solution (6).

4. The culture bottle according to claim 1, wherein The first bottle cap (1) is provided with a first breathing membrane (2), and the second bottle cap (8) is provided with a second breathing membrane (9).

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