Two-layer automatic solid-phase extraction instrument

Abstract

The utility model discloses a two-layer automatic solid-phase extraction instrument which sequentially comprises a first extraction column platform, a second extraction column platform and a collection platform from top to bottom, wherein the first extraction column platform is used for mounting a first extraction column array; the second extraction column platform is used for mounting a second extraction column array and a first collection pipe array; a first liquid adding platform and a second liquid adding platform are respectively arranged above the first extraction column platform and the second extraction column platform, liquid adding pipes are arranged on the first liquid adding platform and the second liquid adding platform, and a front-back moving mechanism for driving the second extraction column array and the first collecting pipe array to move front and back is arranged on the second extraction column platform; according to the scheme, double-column continuous solid-phase extraction of a complex sample can be realized through the first extraction column platform and the second extraction column platform; meanwhile, the scheme can realize rapid collection of primary elution curve component liquid and secondary elution curve component liquid of double-column continuous solid-phase extraction, helps researchers to understand the separation condition of each component in a sample, and qualitatively and quantitatively analyzes a target object.

Description

TECHNICAL FIELD

[0001] The utility model relates to chemical sample pretreatment equipment technical field, concretely relates to a two -layer automatic solid phase extraction appearance. BACKGROUND

[0002] Solid phase extraction technology is a key sample pretreatment technology to realize the separation and enrichment of target components and interfering components in a sample. Typical solid phase extraction operations include multiple steps such as extraction column equilibrium, sample column loading, elution, elution and extraction component collection. These operation steps are tedious, repetitive and time-consuming, and the reagents such as organic solvents used may pose a risk to the health of the operator. Therefore, an automatic solid phase extraction instrument emerges as the times require, aiming to automatically complete these tedious steps, improve sample processing efficiency, and reduce the health risk of personnel.

[0003] In the prior art, the disclosed automatic solid phase extraction instruments can be mainly divided into two categories according to the arrangement layers of the extraction columns: single-layer solid phase extraction instrument and multi-layer solid phase extraction instrument. The single-layer solid phase extraction instrument is provided with only one layer of extraction column, and each sample is subjected to solid phase extraction treatment once. However, for a sample with complex components and various interfering components, it is difficult to completely separate the target components and interfering components by solid phase extraction treatment once.

[0004] The multi-layer solid phase extraction instrument is provided with two or more layers of extraction columns, and each sample can be subjected to multiple continuous solid phase extraction treatments, thereby better solving the separation and enrichment problem of complex samples. However, the currently disclosed multi-layer solid phase extraction instrument design has obvious defects. For example, a four-layer solid phase extraction device with application number CN202411227354.X is provided with only one extraction column in each layer, and the sample is sequentially treated by the four layers of extraction columns from top to bottom, and four components can be collected. The structural feature of the device is that the four layers of extraction columns from top to bottom are fixedly connected through the connecting heads of two-way valves (such as electromagnetic valves or pneumatic valves). This design has the following main disadvantages:

[0005] 1. Small number of samples treated at a time: since there is only one extraction column in each layer, the device can only treat one sample at a time, the throughput is extremely low, and it cannot meet the demand of large-scale sample pretreatment.

[0006] 2. Single component collected in each layer: each layer of extraction column can only collect one component, the flexibility is poor, and it cannot meet the experimental requirements of collecting multiple different components.

[0007] 3. Risk of cross-contamination and inconvenience of cleaning: the extraction columns in each layer are connected through fixed connecting pipelines and two-way valves. The internal structure of these pipelines is complex, it is difficult to clean thoroughly, and the residual sample or reagent can easily cause cross-contamination to the next sample being treated, affecting the accuracy of the analysis results.

[0008] In summary, the single-layer solid phase extraction instrument in the prior art cannot meet the multiple extraction requirements of complex samples, and the existing multi-layer solid phase extraction instrument has problems such as low processing flux, single component collection, and cross-contamination risk. Therefore, there is an urgent need in the field for a two-layer automatic solid phase extraction instrument that can simultaneously achieve high flux, multi-component collection, avoid cross-contamination, and be suitable for complex sample processing. Utility model content

[0009] In view of the above shortcomings of the prior art, the utility model provides a two-layer automatic solid phase extraction instrument, which realizes high flux, multi-component collection of double-column continuous solid phase extraction, and effectively avoids the cross-contamination risk caused by the flow path between the layers.

[0010] To achieve the above-mentioned purpose, the technical scheme adopted by the utility model is:

[0011] The utility model provides a two-layer automatic solid phase extraction instrument, which comprises a first extraction column platform for installing a first extraction column array, a second extraction column platform for installing a second extraction column array and a first collection tube array, and a collection platform from top to bottom, first liquid adding platforms and second liquid adding platforms are arranged above the first extraction column platform and the second extraction column platform, liquid adding tubes are arranged on the first liquid adding platforms and the second liquid adding platforms, and front-back moving mechanisms for driving the second extraction column array and the first collection tube array to move forward and backward and align with the first extraction column array or the second liquid adding platform upward and downward are arranged on the second extraction column platform.

[0012] Further, the first extraction column array and the second extraction column array respectively comprise a plurality of first-layer extraction columns and a plurality of second-layer extraction columns arranged linearly.

[0013] Further, the first-layer extraction columns and the second-layer extraction columns are open-top extraction columns with open tops at upper ends.

[0014] Further, left-right moving mechanisms for driving the liquid adding tubes to move left and right are arranged on the first liquid adding platforms and the second liquid adding platforms, and the liquid adding tubes are connected with a quantitative injection system.

[0015] Further, the collection platform is a one-dimensional collection rack capable of moving forward and backward or a two-dimensional collection rack capable of moving forward and backward and left and right, the one-dimensional collection rack is provided with a second collection tube array, and the two-dimensional collection rack is provided with a elution curve collection container.

[0016] Further, the first collection tube array and the second collection tube array each comprise a plurality of collection tubes arranged in a matrix form at intervals, and the elution curve collection container comprises a plurality of collection tubes arranged in a matrix form closely.

[0017] Further, the first extraction column platform and the second extraction column platform are movably arranged on lifting columns.

[0018] The utility model discloses the beneficial effect is:

[0019] 1. This scheme can realize double column continuous solid phase extraction of complex sample through first extraction column platform and second extraction column platform, and simultaneously, adopts linear arrangement's multichannel parallel extraction design, greatly improves sample pretreatment efficiency, is especially applicable to large -scale sample pretreatment scene of environmental monitoring, food safety, drug analysis etc. Field, in addition, this scheme can realize the rapid collection of the component liquid of the first elution curve and the component liquid of the second elution curve of double column continuous solid phase extraction, and helps researcher to understand the separation of each component in sample, qualitative and quantitative analysis target.

[0020] 2. This scheme cancels the connecting pipeline between the first layer extraction column and the second layer extraction column, adopts the open type extraction column and the liquid flow transmission mode of gravity drop, fundamentally eliminates the cross contamination risk caused by sample residue in complex connecting pipeline, and simultaneously, the simplified flow path is more easy to clean and maintain.

[0021] 3. This scheme is arranged before and after the second extraction column array and the first collection pipe array, so that the effluent of the first layer extraction column can selectively enter the second layer extraction column for secondary extraction, or directly enter the first collection pipe as the final component, realizes the flexible configuration of flow, and through one-dimensional collection frame or two-dimensional collection frame, this scheme can switch between efficient batch processing mode and fine elution curve analysis mode, meets the demand of different experimental purposes. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or prior art, the following will briefly introduce the drawings needed to be used in the embodiments, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without paying creative labor.Through the drawings shown, the above and other purposes, features and advantages of the utility model will be more clear.In all drawings, the same reference signs indicate the same parts.Not deliberately drawn to scale according to actual size, the emphasis is on showing the main idea of the utility model.

[0023] Fig. 1 It is the structural schematic diagram of two layers automatic solid phase extraction instrument.

[0024] Fig. 2 It is the side view of two layers automatic solid phase extraction instrument.

[0025] Fig. 3 It is the structural schematic diagram of one-dimensional collection frame.

[0026] Fig. 4 It is the structural schematic diagram of two-dimensional collection frame.

[0027] Wherein, 1, the first extraction column array, 2, the first extraction column platform, 3, the second extraction column array, 4, the first collection tube array, 5, the second extraction column platform, 6, the collection platform, 7, the first liquid adding platform, 8, the second liquid adding platform, 9, the liquid adding tube, 10, the front and rear moving mechanism, 11, the left and right moving mechanism, 12, the one-dimensional collection rack, 13, the two-dimensional collection rack, 14, the second collection tube array, 15, the elution curve collection container, 16, the lifting column, 17, the upper fixed plate, 18, the lower fixed plate, 19, the fixed block. DETAILED DESCRIPTION

[0028] To make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings herein can be arranged and designed in various different configurations.

[0029] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of the present application.

[0030] It should be noted that: similar reference numbers and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0031] In addition, the terms "first", "second" and the like are only used to distinguish description, and cannot be understood as indicating or implying relative importance.

[0032] As Figs. 1 to 4 shown, the two-layer automatic solid-phase extraction instrument of the present scheme includes, from top to bottom, the first extraction column platform 2 for installing the first extraction column array 1, the second extraction column platform 5 for installing the second extraction column array 3 and the first collection tube array 4, and the collection platform 6, the upper part of the first extraction column platform 2 and the second extraction column platform 5 is respectively provided with the first liquid adding platform 7 and the second liquid adding platform 8 through the fixed block 19, and the first liquid adding platform 7 and the second liquid adding platform 8 are both provided with the liquid adding tube 9, the first extraction column platform 2 and the second extraction column platform 5 can realize double-column continuous solid-phase extraction of complex samples.

[0033] In specific implementation, the collection platform 6 is a one-dimensional collection rack 12 capable of moving forward and backward or a two-dimensional collection rack 13 capable of moving forward and backward and left and right, the one-dimensional collection rack 12 is provided with a second collection tube array 14 for collecting the liquid flowing out of the second extraction column, and the two-dimensional collection rack 13 is provided with a elution curve collection container 15; the first collection tube array 4 and the second collection tube array 14 each include a plurality of collection tubes arranged in a matrix; and the elution curve collection container 15 includes a plurality of collection tubes arranged in a matrix.

[0034] The upper end of the second extraction column platform 5 is provided with an upper fixed plate 17 for mounting the second layer of extraction columns and the first collection tube array 4, and the lower end of the second extraction column platform 5 is provided with a lower fixed plate 18 for fixing the outlet of the second layer of extraction columns; the upper fixed plate 17 and the lower fixed plate 18 can be connected by a fixing member to form an assembled whole capable of moving forward and backward.

[0035] The second extraction column array 3 and the first collection tube array 4 are distributed forward and backward on the second extraction column platform 5, and the second extraction column platform 5 is provided with a forward and backward moving mechanism 10 for driving the second extraction column array 3 and the first collection tube array 4 to move forward and backward and be aligned with the first extraction column array 1 or the second liquid adding platform 8 upward and downward; when the components flowing out of the first layer of extraction columns need to enter the second layer of extraction columns, the second layer of extraction columns is aligned with the first layer of extraction columns upward and downward; when the components of the first layer of extraction columns need to be collected, the first collection tube array 4 is aligned with the first layer of extraction columns upward and downward; when liquid needs to be added to the second layer of extraction columns or elution operation needs to be performed, the second extraction column array 3 is aligned with the liquid adding tube 9 on the second liquid adding platform 8 upward and downward.

[0036] The first extraction column array 1 and the second extraction column array 3 each include ten first layer extraction columns and ten second layer extraction columns arranged in a line; the present scheme adopts a multi-channel parallel extraction design arranged in a line, which greatly improves the sample pretreatment efficiency and is particularly suitable for large-scale sample pretreatment scenes in the fields of environmental monitoring, food safety, drug analysis, etc.

[0037] The first layer of extraction columns and the second layer of extraction columns are both open-top extraction columns with open tops at the upper ends, and the connecting pipelines between the first layer of extraction columns and the second layer of extraction columns are cancelled; the open-top extraction columns and the gravity dripping type liquid flow transmission mode are adopted, which fundamentally eliminates the cross-contamination risk caused by sample residues in the complex connecting pipelines; at the same time, the simplified flow path is easier to clean and maintain.

[0038] The first liquid adding platform 7 and the second liquid adding platform 8 are provided with a left and right moving mechanism 11 for driving the liquid adding tube 9 to move left and right, so as to accurately displace the liquid adding tube 9 above the first layer of extraction columns or the second layer of extraction columns for liquid adding; the liquid adding tube 9 is connected with a quantitative injection system, and the two liquid adding platforms share the same quantitative injection system.

[0039] In particular, the forward and backward moving mechanism 10 and the left and right moving mechanism 11 of this solution can be implemented by existing linear reciprocating motion equipment such as linear modules, drivers, and slide rails, and their specific structures will not be described in detail.

[0040] The first extraction column platform 2 and the second extraction column platform 5 are both movable and height-adjustable on the lifting column 16. Specifically, a driver can be provided on the first extraction column platform 2 and the second extraction column platform 5 and cooperate with the lifting column 16 to realize the lifting and lowering of the first extraction column platform 2 and the second extraction column platform 5 respectively, so as to adapt to the first layer extraction column and the second layer extraction column of different lengths.

[0041] The three working modes of this solution are explained in detail below:

[0042] The first working mode is batch processing mode, which includes the following steps:

[0043] S1: Activation, the first liquid addition platform 7 and the second liquid addition platform 8 inject reagents into the first extraction column and the second extraction column respectively through the liquid addition tube 9, and the first collection tube array 4 and the second collection tube array 14 are used to collect liquids.

[0044] S2: Sample loading, loading the sample into the first extraction column;

[0045] S3: Eluting. The first liquid addition platform 7 injects the eluent into the first extraction column through the liquid addition tube 9. The second extraction column collects the components that need to be further separated. The first collection tube array 4 collects the remaining components. The liquid in the second extraction column flows into the second collection tube array 14. The second liquid addition platform 8 injects the eluent into the second extraction column through the liquid addition tube 9 and collects the component liquid through the second collection tube array 14.

[0046] S4: Cleaning, the first liquid addition platform 7 and the second liquid addition platform 8 inject cleaning reagent into the first extraction column and the second extraction column respectively through the liquid addition tube 9. The first collection tube array 4 and the second collection tube array 14 collect the waste liquid.

[0047] The second working method involves collecting the primary solid-phase extraction eluent in the elution curve mode, which includes the following steps:

[0048] A1: Extraction column adjustment: Remove the first layer of extraction column and place it in the position corresponding to the second layer of extraction column, and select the two-dimensional collection rack 13 for collection platform 6;

[0049] A2: Activation, the second liquid addition platform 8 injects the reagent into the middle part of the first layer extraction column through the liquid addition tube 9, and the elution curve collection container 15 is used to collect the liquid;

[0050] A3: Sample loading, loading the sample into the middle section of the first extraction column;

[0051] A4: Eluting, the second liquid addition platform 8 injects the eluent into the middle part of the first layer extraction column through the liquid addition tube 9, the two-dimensional collection rack 13 works, and the component liquid enters the collection tube in the elution curve collection container 15;

[0052] A5: Cleaning. The second liquid addition platform 8 injects the cleaning solution into the middle part of the first layer extraction column through the liquid addition pipe 9, and collects the waste liquid through the collection pipe in the rinsing curve collection container 15.

[0053] The third working method involves collecting the secondary solid-phase extraction eluent in the elution curve mode, which includes the following steps:

[0054] B1: Activation, the first liquid addition platform 7 and the second liquid addition platform 8 inject reagents into the middle part of the first layer extraction column and the middle part of the second layer extraction column respectively through the liquid addition tube 9. The first collection tube array 4 and the elution curve collection container 15 are used to collect liquids.

[0055] B2: Sample loading, loading the sample into the middle section of the first extraction column;

[0056] B3: Eluting. The first injection platform injects the eluent into the middle section of the first extraction column via the injection tube 9. The middle section of the second extraction column collects the components that need further separation. The collection tubes in the first collection tube array 4 collect the remaining components. The second injection platform injects the eluent into the middle section of the second extraction column via the injection tube 9. The elution curve collection container 15 collects the component liquid.

[0057] B4: Cleaning. The first and second injection platforms inject the cleaning solution into the middle portion of the first extraction column and the middle portion of the second extraction column respectively through the injection pipe 9. The first collection tube array 4 and the rinsing curve collection container 15 are used to collect the waste liquid.

[0058] In summary, this scheme enables rapid collection of primary and secondary elution curve components from dual-column continuous solid-phase extraction, helping researchers understand the separation of components in the sample and perform qualitative and quantitative analysis of target analytes. Furthermore, the sequential arrangement of the second extraction column array 3 and the first collection tube array 4 allows the effluent from the first extraction column to selectively enter the second extraction column for secondary extraction or directly enter the first collection tube as the final component, achieving flexible process configuration. Finally, the use of a one-dimensional collection rack 12 or a two-dimensional collection rack 13 allows the scheme to switch between efficient batch processing and precise elution curve analysis modes, meeting the needs of different experimental objectives.

[0059] Although the specific embodiments of the utility model have been described in detail with reference to the accompanying drawings, they should not be construed as limiting the scope of protection of this patent; various modifications and variations that can be made by those skilled in the art without inventive effort within the scope described in the claims are still within the scope of protection of this patent.

Claims

1. A two-layer automated solid-phase extraction instrument, characterized in that, From top to bottom, the system includes a first extraction column platform for mounting a first extraction column array, a second extraction column platform for mounting a second extraction column array and a first collection tube array, and a collection platform for collecting the component liquid of the second extraction column array. Above the first extraction column platform and the second extraction column platform, a first liquid addition platform and a second liquid addition platform are respectively provided. Both the first liquid addition platform and the second liquid addition platform are provided with liquid addition tubes. The second extraction column platform is provided with a forward and backward movement mechanism for driving the second extraction column array and the first collection tube array to move back and forth and align with the first extraction column array or the second liquid addition platform vertically.

2. The two-layer automated solid-phase extraction apparatus according to claim 1, characterized in that, The first extraction column array and the second extraction column array each include a plurality of first-layer extraction columns and a plurality of second-layer extraction columns arranged linearly.

3. The two-layer automated solid-phase extraction apparatus according to claim 2, characterized in that, Both the first and second extraction columns are open-top extraction columns.

4. The two-layer automated solid-phase extraction apparatus according to claim 3, characterized in that, The first and second liquid addition platforms are equipped with left and right moving mechanisms for driving the liquid addition tube to move left and right, and the liquid addition tube is connected to the quantitative injection system.

5. The two-layer automated solid-phase extraction apparatus according to claim 1, characterized in that, The collection platform is a one-dimensional collection rack that can move forward and backward or a two-dimensional collection rack that can move forward and backward and left and right. The one-dimensional collection rack is provided with a second collection tube array, and the two-dimensional collection rack is provided with a rinsing curve collection container.

6. The two-layer automated solid-phase extraction apparatus according to claim 5, characterized in that, The first and second collection tube arrays each include a plurality of collection tubes arranged in a matrix at intervals, and the rinsing curve collection container includes a plurality of collection tubes arranged in a matrix closely.

7. The two-layer automated solid-phase extraction apparatus according to claim 1, characterized in that, Both the first extraction column platform and the second extraction column platform are movably mounted on the lifting column.

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