Stem cell extraction constant-temperature culture device

Abstract

The utility model provides a stem cell extraction constant-temperature culture device which comprises a main body mechanism and a culture mechanism arranged in the main body mechanism, the culture mechanism comprises a motor, a limiting box arranged at the output end of the motor, limiting gauzes arranged in the two sides of the limiting box, and a base arranged in the limiting box, the supporting blocks are arranged at the four corners of the top of the base, and the fixing pieces are arranged at the tops of the supporting blocks; when the stem cell culture box is used, after stem cells enter the culture box, the cover plate is rotated to enable the cover plate and the threaded ring at the top of the culture box to be screwed and fixed, the culture box is placed in the fixing piece and the limiting groove in the base, the base is placed in the limiting box for centralized culture, and meanwhile the motor drives the limiting box to rotate; meanwhile, when the stem cells are taken out, the culture box is independently taken out for taking the stem cells, and waste and pollution caused by excessive taking are prevented.

Description

Technical Field

[0001] This utility model relates to the field of cell extraction technology, and in particular to a constant temperature culture device for stem cell extraction. Background Technology

[0002] Stem cells, possessing self-renewal capacity and multipotent differentiation potential, have demonstrated immense application potential in regenerative medicine and disease treatment. Based on their developmental stage, stem cells can be divided into embryonic stem cells and adult stem cells. According to their developmental potential, they can be further classified into totipotent stem cells, pluripotent stem cells, and unipotent stem cells. These primitive and unspecialized cells possess the potential to regenerate various tissues and organs, exist in all multicellular tissues, and can form various specialized cell types through mitosis and differentiation. They replenish their numbers through self-renewal. In stem cell research and application, isothermal culture is a crucial step in maintaining stem cell activity and promoting their growth and differentiation. A stable and suitable temperature environment is essential for the normal physiological functions, gene expression, and cell cycle progression of stem cells, ensuring that they proliferate and differentiate in the expected direction, providing high-quality cell resources for subsequent scientific research and clinical applications.

[0003] Existing constant-temperature culture devices for stem cell extraction suffer from uneven heating of stem cells, leading to displacement of the regeneration site. Furthermore, the extraction of large quantities of stem cells at once during the extraction process can result in waste and contamination.

[0004] To address this, a constant-temperature culture device for stem cell extraction is proposed. Utility Model Content

[0005] In view of this, the present invention aims to provide a stem cell extraction and constant temperature culture device to solve or alleviate the technical problems existing in the prior art, and at least provide a beneficial option.

[0006] The technical solution of this utility model embodiment is implemented as follows: A stem cell extraction constant temperature culture device includes a main body and a culture mechanism disposed inside the main body. The culture mechanism includes: a motor, a limiting box disposed at the output end of the motor, limiting mesh disposed inside both sides of the limiting box, a base disposed inside the limiting box, support blocks disposed at the four corners of the top of the base, a fixing plate disposed at the top of the support block, a limiting groove disposed inside the base and the fixing plate, a culture box disposed inside the limiting groove, a threaded ring disposed at the top of the culture box, and a cover plate disposed on the outer wall of the threaded ring.

[0007] In some embodiments, the culture box is provided with a culture tank inside, the top of the cover plate is provided with a hand-held piece, and the hand-held piece is provided with rubber sheets on both sides.

[0008] In some embodiments, the top of the limiting box is provided with a snap-fit ​​sleeve, the inside of the snap-fit ​​sleeve is provided with a breathable mesh, and a transparent door is provided on one side of the bottom of the snap-fit ​​sleeve.

[0009] In some embodiments, the main body includes a rotating disk disposed at the bottom of the limiting box, a fixing plate disposed on the outer wall of the rotating disk, and an incubator disposed on the outer wall of the fixing plate.

[0010] In some embodiments, the incubator is provided with a top plate, a motor is provided on the top of the top plate, and a rotating rod is provided at the output end of the motor.

[0011] In some embodiments, a working box is provided at the bottom of the incubator, and a door is provided on one side of the incubator.

[0012] The present invention has the following advantages due to the adoption of the above technical solution:

[0013] A constant-temperature culture device for stem cell extraction is disclosed. In use, after the stem cells are placed inside the culture box, the cover is rotated to tighten and fix the cover to the threaded ring on the top of the culture box. The culture box is then placed into the limiting groove inside the fixing plate and the base. The base is then placed inside the limiting box for centralized culture. At the same time, the motor drives the limiting box to rotate, so that the stem cells inside are heated evenly. When removing the stem cells, the culture box is taken out separately to prevent excessive removal, waste, and contamination.

[0014] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a cross-sectional structural diagram of the present invention;

[0017] Figure 2 This is an overall structural diagram of the present invention;

[0018] Figure 3 This is a cross-sectional view of the limiting box of this utility model;

[0019] Figure 4This is a structural diagram of the base of this utility model;

[0020] Figure 5 This is a structural diagram of the culture box of this utility model.

[0021] Figure label:

[0022] 100. Main structure; 101. Incubator; 102. Top plate; 103. Motor; 104. Fixing plate; 105. Rotating disc; 106. Working box; 107. Box door; 108. Rotating rod; 200. Culture mechanism; 201. Motor; 202. Limiting box; 203. Limiting mesh; 204. Base; 205. Support block; 206. Fixing piece; 207. Limiting groove; 208. Culture box; 209. Culture tank; 210. Threaded ring; 211. Cover plate; 212. Handheld piece; 213. Rubber sheet; 214. Transparent door; 215. Snap-fit ​​sleeve; 216. Breathable mesh. Detailed Implementation

[0023] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0024] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0025] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0026] Example 1:

[0027] like Figure 1-5As shown, a stem cell extraction and constant temperature culture device includes a main body 100 and a culture mechanism 200 installed inside the main body 100. The culture mechanism 200 includes: a motor 201; a limiting box 202 installed at the output end of the motor 201; limiting mesh 203 snapped into the inside of both sides of the limiting box 202; a base 204 placed inside the limiting box 202; support blocks 205 installed at the four corners of the top of the base 204; fixing plates 206 installed on the top of the support blocks 205; limiting grooves 207 formed inside the base 204 and the fixing plates 206; and a culture box placed inside the limiting grooves 207. 208. Place the base 204 into the limiting box 202 for centralized culture. At the same time, the motor 201 drives the limiting box 202 to rotate, so that the internal stem cells are heated evenly. When taking them out, take out the culture box 208 separately to take out the stem cells. The threaded ring 210 installed on the top of the culture box 208 is threaded to the cover plate 211 on the outer wall of the threaded ring 210. After the stem cells are put into the culture box 208, rotate the cover plate 211 to tighten and fix the cover plate 211 to the threaded ring 210 on the top of the culture box 208. Place the culture box 208 into the limiting groove 207 inside the fixing plate 206 and the base 204.

[0028] In this embodiment, a culture tank 209 is provided inside the culture box 208, and a hand-held piece 212 is installed on the top of the cover plate 211. Rubber pieces 213 are glued to both sides of the hand-held piece 212 to facilitate picking up the culture box 208. At the same time, the cover plate 211 can be unscrewed from the top of the culture box 208 by twisting the hand-held piece 212.

[0029] In this embodiment, a snap-fit ​​sleeve 215 is installed on the top of the limiting box 202, and a breathable mesh 216 is installed inside the snap-fit ​​sleeve 215 to ensure gas flow. A transparent door 214 is provided on one side of the bottom of the snap-fit ​​sleeve 215 for easy observation.

[0030] In this embodiment, the main body 100 includes a rotating disk 105 rotatably connected to the bottom of the limiting box 202, a fixed plate 104 with a rotating rod connected to the outer wall of the rotating disk 105, and an incubator 101 installed on the outer wall of the fixed plate 104. A top plate 102 is installed on the top of the incubator 101, and a motor 103 is installed on the top of the top plate 102. A rotating rod 108 is installed at the output end of the motor 103. The motor 103 drives the rotating disk 105 on the outer wall of the rotating rod 108 to rotate, thereby driving the limiting box 202 to rotate.

[0031] In this embodiment, a working box 106 is installed at the bottom of the incubator 101, and a door 107 is rotatably connected to one side of the incubator 101 for easy placement and retrieval.

[0032] In this embodiment: When using the product, after the stem cells are placed inside the culture box 208, the cover plate 211 is rotated to tighten and fix the cover plate 211 to the threaded ring 210 on the top of the culture box 208. The culture box 208 is then placed inside the limiting groove 207 inside the fixing plate 206 and the base 204. The base 204 is then placed inside the limiting box 202 for centralized culture. At the same time, the motor 201 drives the limiting box 202 to rotate, so that the stem cells inside are heated evenly. When taking them out, the culture box 208 is taken out separately to take out the stem cells, preventing waste and contamination caused by taking out too many.

[0033] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this utility model, and these should all be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A stem cell extraction and isothermal culture device, comprising a main body (100) and a culture mechanism (200) disposed inside the main body (100), characterized in that: The culture mechanism (200) includes: a motor (201), a limiting box (202) disposed at the output end of the motor (201), limiting mesh (203) disposed inside both sides of the limiting box (202), a base (204) disposed inside the limiting box (202), support blocks (205) disposed at the four corners of the top of the base (204), a fixing piece (206) disposed at the top of the support block (205), a limiting groove (207) disposed inside the base (204) and the fixing piece (206), a culture box (208) disposed inside the limiting groove (207), a threaded ring (210) disposed at the top of the culture box (208), and a cover plate (211) disposed on the outer wall of the threaded ring (210).

2. The stem cell extraction and isothermal culture device according to claim 1, characterized in that: The culture box (208) is provided with a culture tank (209) inside, and a hand-held piece (212) is provided on the top of the cover plate (211), and rubber pieces (213) are provided on both sides of the hand-held piece (212).

3. The stem cell extraction and isothermal culture device according to claim 2, characterized in that: The top of the limiting box (202) is provided with a snap-fit ​​sleeve (215), the inside of the snap-fit ​​sleeve (215) is provided with a breathable mesh (216), and a transparent door (214) is provided on one side of the bottom of the snap-fit ​​sleeve (215).

4. The stem cell extraction and isothermal culture device according to claim 3, characterized in that: The main body (100) includes a rotating disk (105) disposed at the bottom of the limiting box (202), a fixing plate (104) disposed on the outer wall of the rotating disk (105), and an incubator (101) disposed on the outer wall of the fixing plate (104).

5. The stem cell extraction and constant temperature culture device according to claim 4, characterized in that: The incubator (101) is provided with a top plate (102), and a motor (103) is provided on the top of the top plate (102). A rotating rod (108) is provided at the output end of the motor (103).

6. The stem cell extraction and isothermal culture device according to claim 5, characterized in that: The incubator (101) has a working box (106) at the bottom and a door (107) on one side.

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