Stem cell culture device

Abstract

The utility model discloses a stem cell culture device which comprises a culture box, a ventilation opening is formed in the inner box wall, facing the box opening of the culture box, of the culture box, the stem cell culture device further comprises opposite groove supporting strips and a layering mechanism, and the opposite groove supporting strips are symmetrically fixed to the positions of the inner box walls on the two sides of the culture box. Through the design of the layering mechanism, stem cell culture vessels can be flexibly placed on shelves with different heights, the space utilization rate in the culture box is improved, the culture vessels are more convenient to take or place, the possibility of misoperation is reduced, and the working efficiency is improved. Meanwhile, the air circulation in the culture box is guaranteed through the forked edge air grooves of the base plate and the forked edge passing grooves of the first layer frame, the second layer frame and the third layer frame, the air quality in the culture box is favorably maintained, and a good growth environment is provided for stem cells.

Description

Technical Field

[0001] This utility model relates to the field of stem cell culture technology, and in particular to a stem cell culture device. Background Technology

[0002] Stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated daughter cells. When culturing stem cells experimentally, cell culture incubators are generally used to culture the culture medium in culture dishes or flasks. A culture incubator is a temperature-controlled chamber device mainly used for culturing microorganisms, plant and animal cells. Some have a two-way temperature control system for both cooling and heating. It is a basic experimental equipment for scientific research departments in biology, agriculture, medicine, environmental protection and other fields.

[0003] A stem cell culture device, disclosed in CN220335214U, includes a culture chamber body with a first placement slot inside. A tray is fixedly installed inside the first placement slot, and three sets of fixing structures are provided on the upper surface of the tray. A sealing door is hinged to the front of the culture chamber body, and a handle is fixedly installed on the side wall of the sealing door. Support legs are fixedly installed at the bottom of the culture chamber body. This invention, by designing fixing structures on a stem cell culture device, reduces the possibility of internal contamination of the culture medium inside the other placement slots when the sealing door is open. The third and second placement slots can hold culture dishes, achieving separate placement of culture medium and culture dishes. This reduces the risk of other culture dishes or flasks being knocked down and damaged during handling, thus preventing losses.

[0004] Existing patented stem cell culture devices have a fixed structure inside the culture chamber for layering culture dishes. However, the ventilation effect of the placement plate of this fixed structure is limited. When used for stem cell culture operations, it will affect the air circulation space inside the culture chamber, thereby affecting the air quality inside the culture chamber and thus affecting the culture of stem cells. To address this, we propose a stem cell culture device. Utility Model Content

[0005] The main objective of this invention is to provide a stem cell culture device that improves the fixed structure of the existing patented culture box into a layered mechanism. This layered mechanism allows stem cell culture vessels to be flexibly placed on shelves of different heights, improving the space utilization inside the culture box and making it more convenient to pick up or place the vessels, reducing the possibility of misoperation. Simultaneously, the cross-edge air channels on the substrate and the cross-edge passageways on the first, second, and third shelves ensure air circulation inside the culture box, helping to maintain air quality and providing a good growth environment for stem cells. This effectively solves the problems in the prior art.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A stem cell culture device includes a culture chamber with an air vent on its inner wall facing its opening. It also includes slot support strips and a layering mechanism. Slot support strips are symmetrically fixed to the inner walls on both sides of the culture chamber. The layering mechanism includes a base plate, a first shelf, a second shelf, a third shelf, a fixed column, an insert plate, and a T-shaped adhesive baffle. The base plate is mounted on the surface of the slot support strips, and the first shelf, second shelf, and third shelf are arranged from low to high on the surface of the base plate. The base plate surface below the first shelf, second shelf, and third shelf... The first, second, and third shelves have fixed holes for mounting fixed columns, and the fixed columns are fixed to the bottom ends of the support rods of the first, second, and third shelves. The shelf surfaces of the first, second, and third shelves facing the incubator opening have grooves for inserting insert plates, and T-shaped adhesive baffles are attached to the surface of the insert plates. The shelf surfaces of the first, second, and third shelves away from the insert plates have rectangular concave surfaces, and forked-edge through grooves are provided through the rectangular concave surfaces of the first, second, and third shelves. Forked-edge air grooves are arranged on the substrate surfaces between the fixed holes.

[0008] Furthermore, the grooves of the forked side air duct are arranged in six groups on the surface of the substrate, and rectangular forked grooves are provided outside the strip-shaped groove cavity of the forked side air duct.

[0009] By adopting the above technical solution, the substrate surface is provided with a number of cross-shaped air channels that can be circulated by the airflow inside the incubator.

[0010] Furthermore, the grooves of the fork-side through groove are arranged and opened along the length direction of the plate on the surface of the first, second and third shelves, and the surface of the first, second and third shelves near the fork-side through groove is provided with a diagonal fork groove.

[0011] By adopting the above technical solution, the surfaces of the first, second, and third shelves have more fork-edge grooves, which improves the air passage capacity of the first, second, and third shelves.

[0012] Furthermore, the upper plate of the insert plate has a symmetrically protruding support rod, and the support rod is inserted upward into the body of the T-shaped rubber baffle.

[0013] By adopting the above technical solution, the insert plate and the support rod can be inserted into the T-shaped rubber baffle to provide support.

[0014] Furthermore, the height of the first shelf is successively less than the height of the second and third shelves, and double-hole shelves are symmetrically arranged on both sides of the shelves of the first, second and third shelves.

[0015] By adopting the above technical solution, the different heights of the first, second, and third shelves make it convenient to remove stem cell culture vessels when placing them on the surface of the first, second, and third shelves.

[0016] Furthermore, the surface of the groove support strip is symmetrically provided with grooves, and the grooves of the groove support strip are fitted with fixed feet protruding from the lower surface of the substrate.

[0017] By adopting the above technical solution, the fixed feet of the substrate can be positioned and clamped in the groove of the slot support strip.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] This invention improves the fixed structure of the existing patented incubator into a layered mechanism. The layered mechanism allows stem cell culture vessels to be flexibly placed on shelves of different heights, improving the space utilization inside the incubator and making it more convenient to pick up or place the culture vessels, thus reducing the possibility of misoperation.

[0020] Furthermore, the rectangular concave design of the first, second, and third shelves, along with the blocking effect of the T-shaped adhesive baffles, effectively prevents other vessels from accidentally falling when the culture vessels are picked up or moved, thus increasing the safety and stability of the stem cell culture process.

[0021] Meanwhile, the cross-shaped air channels on the substrate, as well as the cross-shaped passage channels on the first, second, and third shelves, ensure air circulation inside the incubator, helping to maintain air quality and providing a good growth environment for stem cells. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the overall structure of a stem cell culture device according to the present invention.

[0023] Figure 2 This is an exploded view of the layered structure of a stem cell culture device according to this utility model.

[0024] Figure 3 This is a schematic diagram of the first layer structure of a stem cell culture device according to the present invention.

[0025] In the diagram: 1. Incubator; 2. Ventilation port; 3. Alignment bar; 4. Layering mechanism; 5. Base plate; 6. Fixed foot; 7. Forked side air duct; 8. First shelf; 9. Second shelf; 10. Third shelf; 11. Fixed column; 12. Fixed hole; 13. Forked side through groove; 14. Strip fixed groove; 15. Insert plate; 16. Support rod; 17. T-shaped glue baffle. Detailed Implementation

[0026] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0027] like Figure 1-3 As shown, a stem cell culture device includes a culture box 1. The culture box 1 has an air vent 2 on its inner wall facing its opening. It also includes slot support strips 3 and a layering mechanism 4. Slot support strips 3 are symmetrically fixed to the inner walls on both sides of the culture box 1. The layering mechanism 4 includes a base plate 5, a first shelf 8, a second shelf 9, a third shelf 10, a fixed column 11, an insert plate 15, and a T-shaped adhesive baffle 17. The base plate 5 is mounted on the surface of the slot support strips 3, and the first shelf 8, second shelf 9, and third shelf 10 are arranged from low to high on the surface of the base plate 5. An opening is formed on the surface of the base plate 5 below the first shelf 8, second shelf 9, and third shelf 10. There are fixed holes 12 for mounting the fixed column 11, and the fixed column 11 is fixed to the bottom end of the frame rod of the first layer frame 8, the second layer frame 9 and the third layer frame 10. The surface of the frame plate of the first layer frame 8, the second layer frame 9 and the third layer frame 10 facing the opening of the incubator 1 is provided with a groove 14 for inserting the insert plate 15, and a T-shaped glue baffle 17 is fixed to the surface of the plate body of the insert plate 15. The surface of the frame plate of the first layer frame 8, the second layer frame 9 and the third layer frame 10 away from the insert plate 15 is provided with a rectangular concave surface, and a forked side through groove 13 is provided through the rectangular concave surface of the first layer frame 8, the second layer frame 9 and the third layer frame 10. Forked side air grooves 7 are arranged on the surface of the substrate 5 between the fixed holes 12.

[0028] Among them, the groove of the forked side air duct 7 is arranged in six groups on the surface of the substrate 5, and the strip-shaped groove of the forked side air duct 7 is provided with a rectangular forked groove.

[0029] By adopting the above technical solution, the surface of the substrate 5 is provided with a number of cross-shaped air grooves 7, which can be circulated by the airflow inside the incubator 1.

[0030] The groove of the fork-side groove 13 is arranged along the length of the plate on the surface of the first shelf 8, the second shelf 9 and the third shelf 10, and the surface of the first shelf 8, the second shelf 9 and the third shelf 10 near the fork-side groove 13 is provided with a diagonal fork groove.

[0031] By adopting the above technical solution, the surfaces of the first shelf 8, the second shelf 9, and the third shelf 10 have more forked side grooves 13, which improves the air passage capacity of the first shelf 8, the second shelf 9, and the third shelf 10.

[0032] The upper plate of the insert plate 15 has a symmetrically protruding support rod 16, and the support rod 16 is inserted upward into the plate body of the T-shaped rubber baffle 17.

[0033] By adopting the above technical solution, the insert plate 15 and the support rod 16 can be inserted into the T-shaped rubber baffle 17 to provide support.

[0034] The height of the first shelf 8 is successively less than the height of the second shelf 9 and the third shelf 10, and double-hole shelves are symmetrically arranged on both sides of the shelves of the first shelf 8, the second shelf 9 and the third shelf 10.

[0035] By adopting the above technical solution, the different layer heights of the first shelf 8, the second shelf 9, and the third shelf 10 make it convenient to remove stem cell culture vessels when they are placed on the surface of the first shelf 8, the second shelf 9, and the third shelf 10.

[0036] Among them, the surface of the groove support strip 3 is symmetrically provided with grooves, and the groove support strip 3 is fitted with a fixed foot 6 protruding from the lower surface of the substrate 5.

[0037] By adopting the above technical solution, the fixed foot 6 of the substrate 5 can be positioned and clamped in the groove of the groove support strip 3.

[0038] It should be noted that this utility model is a stem cell culture device. By improving the fixing structure of the existing patented culture box 1 to a layered mechanism 4, the base plate 5 of the layered mechanism 4 can be snapped into the groove support strip 3 inside the culture box 1. Then, the first layer 8, the second layer 9, and the third layer 10 can be snapped into the surface of the base plate 5 one by one from bottom to top. Then, the insertion plate 15 can be snapped into the groove 14 of the first layer 8, the second layer 9, and the third layer 10. At the same time, the insertion plate 15 is snapped into the T-shaped adhesive strip 17 by the support rod 16. The first layer 8, the second layer 9, and the third layer 10 The layered rack has rectangular concave surfaces for placing stem cell culture vessels. If other vessels are accidentally bumped during the handling of stem cell culture vessels, the rectangular concave surfaces and T-shaped adhesive baffles 17 will prevent them from falling out of the first rack 8, second rack 9, and third rack 10. At the same time, the cross-shaped passage grooves 13 at the first rack 8, second rack 9, and third rack 10, as well as the cross-shaped air grooves 7 at the substrate 5, have good ventilation capabilities, ensuring air circulation inside the incubator 1, ensuring good air circulation effect of the air circulation system inside the incubator 1, and ensuring air quality during stem cell culture.

[0039] It should be noted that this utility model is a stem cell culture device. All components in this utility model are known to those skilled in the art, and their structure and principle can be learned by those skilled in the art through technical manuals or conventional experimental methods.

[0040] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A stem cell culture device, comprising a culture chamber (1), wherein the culture chamber (1) has an air vent (2) on its inner wall facing its own opening, characterized in that: It also includes a slot support strip (3) and a layering mechanism (4). The slot support strip (3) is symmetrically fixed on both sides of the inner wall of the incubator (1). The layering mechanism (4) includes a base plate (5), a first shelf (8), a second shelf (9), a third shelf (10), a fixed column (11), an insert plate (15), and a T-shaped adhesive baffle (17). The base plate (5) is attached to the surface of the slot support strip (3). The first shelf (8), the second shelf (9), and the third shelf (10) are arranged from low to high on the surface of the base plate (5). The base plate (5) below the first shelf (8), the second shelf (9), and the third shelf (10) have fixed holes (12) for mounting the fixed column (11). The bottom of the support rods of the first layer (8), the second layer (9) and the third layer (10) are fixed. The first layer (8), the second layer (9) and the third layer (10) have grooves (14) for inserting the insert plate (15) on the side of the support plate facing the incubator (1). The plate surface of the insert plate (15) is fitted with a T-shaped glue baffle (17). The support plate surface of the first layer (8), the second layer (9) and the third layer (10) away from the insert plate (15) is provided with a rectangular concave surface. The rectangular concave surface of the first layer (8), the second layer (9) and the third layer (10) is provided with a forked side through groove (13). The base plate (5) between the fixed holes (12) is provided with forked side air grooves (7).

2. The stem cell culture device according to claim 1, characterized in that: The groove of the forked side air duct (7) is arranged in six groups on the surface of the substrate (5), and the strip-shaped groove of the forked side air duct (7) is provided with a rectangular forked groove.

3. The stem cell culture device according to claim 1, characterized in that: The groove of the fork-side through groove (13) is arranged along the length of the plate on the surface of the first layer (8), the second layer (9) and the third layer (10), and the surface of the first layer (8), the second layer (9) and the third layer (10) near the fork-side through groove (13) is provided with a diagonal fork groove.

4. The stem cell culture device according to claim 1, characterized in that: The upper plate of the insert plate (15) has a symmetrically protruding support rod (16), and the support rod (16) is inserted upward into the plate body of the T-shaped rubber baffle (17).

5. A stem cell culture device according to claim 1, characterized in that: The height of the first shelf (8) is successively less than the height of the second shelf (9) and the third shelf (10), and double-hole shelves are symmetrically arranged on both sides of the shelf panels of the first shelf (8), the second shelf (9) and the third shelf (10).

6. The stem cell culture device according to claim 1, characterized in that: The groove support strip (3) has symmetrical grooves on its surface, and the groove support strip (3) has a fixed foot (6) protruding from the lower surface of the substrate (5) inserted into the groove.

Images