Microorganism separation and inspection device

Abstract

The utility model discloses a microorganism separation inspection device which comprises a left shell, a right shell and a centrifugal mechanism, a bottom plate, a limiting plate and a top plate are sequentially arranged in the left shell from bottom to top, a semicircular motor groove is formed in the center of the bottom plate, and a semicircular limiting groove is formed in the center of the limiting plate; a semicircular groove is formed in the center of the top plate, the inner structure of the right shell is the same as that of the left shell, the motor grooves are combined and spliced into a circular groove, and the limiting groove and the semicircular groove are aligned and spliced to form a limiting hole and a circular hole respectively; according to the device, a reagent tube filled with microorganisms is arranged on the centrifugal plate, the device can be used after being covered with a glass cover, the device is very convenient, the separated microorganisms can be directly placed in a culture dish for culture research, convenience is provided for scientific researchers, the device is more practical, meanwhile, the device can be detached and cleaned, and the cost is low. The influence on research results of scientific researchers caused by bacteria breeding in the device is avoided.

Description

A microbial isolation and testing device Technical Field

[0001] This utility model belongs to the field of microbial isolation technology, specifically relating to a microbial isolation and testing device. Background Technology

[0002] Microorganisms play a vital role in medicine, environment, industry, and agriculture. For example, they are used in medicine for antibiotic development, in the environment for pollution control, in industry for fermentation production, and in agriculture for promoting plant growth. Microbial isolation technology is fundamental to the study of microorganisms; only by isolating pure cultures can their characteristics be studied in depth. At the application level, isolation technology helps in the discovery of new species, the development of new drugs, the optimization of industrial production strains, and environmental monitoring and bioremediation.

[0003] In existing technologies, microorganisms are mainly separated by centrifugation, with tubular centrifuges being the most common. Although these centrifuges are highly efficient and can be used in pharmaceuticals, chemicals, and biotechnology, they are cumbersome for scientific research. They are not only bulky but also can only separate large quantities at a time. In scientific research, only a small amount is usually needed to be cultured in petri dishes, but tubular centrifuges can separate several tubs of microbial solution at once, resulting in a supply exceeding demand. This makes it difficult for researchers to process the remaining microbial solution after use. Summary of the Invention

[0004] To overcome the problems in the background art, this utility model provides a microbial isolation and testing device.

[0005] To achieve the above objectives, this utility model is implemented through the following technical solution:

[0006] A microbial isolation and testing device includes: a left outer shell, a right outer shell, and a centrifugation mechanism. The interior of the left outer shell is provided with a bottom plate, a limiting plate, and a top plate from bottom to top. The bottom plate has a semi-circular motor slot at its center, the limiting plate has a semi-circular limiting slot at its center, and the top plate has a semi-circular semi-circular slot at its center. The internal structure of the right outer shell is the same as that of the left outer shell. The motor slot is assembled into a circular slot, and the limiting slot and the semi-circular slot are aligned and assembled to form a limiting hole and a circular hole, respectively. The interior of the left outer shell is provided with a battery compartment.

[0007] The centrifuge mechanism includes a motor, a connecting sleeve, a transmission rod, a bearing, a centrifuge plate, and a fixing cover. The motor is installed in the motor slot, and the connecting sleeve is fixed to the top of the motor's output shaft. The transmission rod is vertically installed at the upper end of the connecting sleeve. The transmission rod passes through a circular hole and a bearing is fitted in the circular hole. The centrifuge plate is fitted to the top of the transmission rod, and a fixing cover is installed on the centrifuge plate to fasten the centrifuge plate to the transmission shaft.

[0008] Preferably, the centrifuge plate has a planar circular base at its center, with a raised portion extending circumferentially upward around the edge of the base. The raised portion forms a continuous arc-shaped surface with the base. A through hole is provided at the center of the base. The top of the drive shaft has a stepped lower truncated cone and an upper truncated cone. The centrifuge plate is installed on the lower truncated cone through the through hole. A thread is provided on the side of the upper truncated cone. A matching thread is provided on the inner side of the fixing cover. After the centrifuge plate is installed on the lower truncated cone, the fixing cover is screwed onto the upper truncated cone to fix the centrifuge plate.

[0009] Preferably, the raised portion has equidistant placement holes around the base, a rubber sleeve is installed in the placement hole, and a reagent tube containing the microbial solution to be separated is inserted into the rubber sleeve.

[0010] Preferably, the inner ring of the bearing is fastened to the shaft of the transmission rod, and a rubber washer is fitted in the gap between the outer ring of the bearing and the circular hole.

[0011] Preferably, the limiting hole is fitted on the side of the motor, and the upper part of the base plate and the lower side of the top plate are provided with screw posts. After the screw posts of the left and right outer shells are assembled, the screw posts are aligned and connected, and screws are screwed in to fix the left and right outer shells together.

[0012] Preferably, a limiting wheel is provided between the limiting plate and the top plate, and a limiting ring is provided at the center of the limiting wheel and fitted onto the rod body of the transmission rod. The outer side of the limiting wheel is a ring and the ring abuts against the inner wall of the left outer shell and the right outer shell. The inner wall of the left outer shell and the right outer shell is provided with a number of limiting blocks, and the limiting blocks are distributed on the upper and lower sides of the ring.

[0013] Preferably, the left and right outer shells are assembled and fixed to form a cylindrical structure, and a glass cover is installed on top to seal the entire protruding centrifugal plate.

[0014] The beneficial effects of this utility model are:

[0015] 1. This utility model uses centrifugal force generated by high-speed rotation of the centrifugal mechanism to physically separate microorganisms in reagent tubes. Compared with traditional tube centrifuges, this device has a small structure, small size, and low cost. Existing tube centrifuges are large and mostly used in factory production, while this device is suitable for use in the field of microbial research.

[0016] 2. This device is very convenient. Simply place the reagent tube containing microorganisms on a centrifuge plate, cover it with a glass cover, and it is ready to use. The isolated microorganisms can be directly placed into a petri dish for culture and research, providing convenience for researchers and making it more practical. At the same time, this device can be disassembled for cleaning, preventing the growth of bacteria inside the device from affecting the research results of researchers. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 is a three-dimensional schematic diagram of the device of this utility model;

[0019] Figure 2 is a schematic diagram of the device structure of this utility model;

[0020] Figure 3 is a schematic diagram of the left outer shell structure of this utility model;

[0021] Figure 4 is an internal cross-sectional view of the device of this utility model;

[0022] Figure 5 is a partial enlarged view of point A in Figure 4 of this utility model;

[0023] Figure 6 is an explosion diagram of the device of this utility model;

[0024] Figure 7 is an explosion diagram of the centrifuge mechanism of this utility model;

[0025] 1-Left outer shell, 11-Bottom plate, 12-Limiting plate, 121-Limiting hole, 13-Top plate, 131-Round hole, 14-Battery compartment, 15-Screw post, 2-Right outer shell, 21-Motor slot, 22-Limiting slot, 23-Semi-circular slot, 24-Limiting block, 3-Centrifuge mechanism, 31-Motor, 32-Connecting sleeve, 33-Transmission rod, 331-Upper frustum, 332-Lower frustum, 34-Bearing, 341-Rubber washer, 35-Centrifuge plate, 351-Base, 352-Raised part, 353-Through hole, 354-Placement hole, 355-Rubber sleeve, 36-Fixing cover, 4-Limiting wheel, 41-Limiting ring, 42-Ring, 5-Glass cover, 6-Reagent tube. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.

[0027] Referring to Figures 1 to 7, this utility model discloses a microbial isolation and testing device, including: a left outer shell 1, a right outer shell 2, and a centrifugation mechanism 3. The interior of the left outer shell 1 is provided with a bottom plate 11, a limiting plate 12, and a top plate 13 from bottom to top. The bottom plate 11 has a semi-circular motor slot 21 at its center, the limiting plate 12 has a semi-circular limiting slot 22 at its center, and the top plate 13 has a semi-circular semi-circular slot 23 at its center. The internal structure of the right outer shell 2 is the same as that of the left outer shell 1. The motor slot 21 is assembled into a circular slot, and the limiting slot 22 and the semi-circular slot 23 are aligned and assembled to form a limiting hole 121 and a circular hole 131, respectively. The left outer shell 1 has a battery compartment 14 inside. In this device, the left outer shell 1 and the right outer shell 2 are the same, except that the left outer shell 1 has an additional battery compartment 14. A battery is added to the battery compartment 14 to provide power to the motor 31. The rest of the structure is the same. This device can be assembled simply by assembling the left outer shell 1 and the right outer shell 2 along their edges.

[0028] The centrifugal mechanism 3 includes a motor 31, a connecting sleeve 32, a transmission rod 33, a bearing 34, a centrifugal plate 35, and a fixing cover 36. The motor 31 is installed in the motor slot 21. The connecting sleeve 32 is fixed to the top of the output shaft of the motor 31. The transmission rod 33 is vertically installed at the upper end of the connecting sleeve 32. The transmission rod 33 passes through the circular hole 131 and the bearing 34 is fitted at the circular hole 131. The centrifugal plate 35 is fitted to the top of the transmission rod 33. At the same time, the fixing cover 36 is installed on the centrifugal plate 35 to fasten the centrifugal plate 35 to the transmission shaft 33.

[0029] It should be noted that, as shown in Figures 4, 6, and 7, the centrifuge mechanism 3 is the most important core component of this device. In use, first assemble the left outer shell 1 and the right outer shell 2, then insert the reagent tube into the centrifuge plate 35 to start the device for separating microorganisms. Start the battery in the battery compartment 14, and the battery will transmit electrical energy to the motor 31. The motor 31 will drive the connecting sleeve 32 to rotate. Since the connecting sleeve 32 is fixedly connected to the transmission rod 33 by threads, the transmission rod 33 will rotate synchronously. The bearing 34 facilitates the free rotation of the transmission rod 33. The transmission rod 33 drives the centrifuge plate 35 to rotate, which in turn causes the reagent tube 6 fixed on the centrifuge plate 35 to rotate at high speed, thereby generating centrifugal force to separate the microorganisms in the reagent tube 6.

[0030] It should be noted that microorganisms are separated by centrifugation mainly because microorganisms are usually denser than other components in the culture medium. Therefore, a precipitate will form at the bottom of the tube after centrifugation, which allows for separation. In scientific research, the differences before and after microbial separation are: firstly, the concentration is increased, and the microorganisms are concentrated, making them easier to observe or analyze; secondly, inhibitors, such as antibiotics or other inhibitors in the culture medium, are removed, which may allow the microorganisms to regain activity or grow more easily. With increased purity, subsequent experimental results will be more accurate. For example, fewer impurities will result in more reliable results in PCR or sequencing.

[0031] In this embodiment, the centrifugal plate 35 has a planar circular base 351 at its center, and a raised portion 352 extends circumferentially around the edge of the base 351 and bends upward. The raised portion 352 and the base 351 form a continuous arc surface. A through hole 353 is provided at the center of the base 351. The top of the drive shaft 33 has a stepped lower truncated cone 332 and an upper truncated cone 331. The centrifugal plate 35 is installed on the lower truncated cone 332 through the through hole 353. The upper truncated cone 331 has threads on its side. The inner side of the fixing cover 36 has matching threads. After the centrifugal plate 35 is installed on the lower truncated cone 331, the fixing cover 36 is screwed onto the upper truncated cone 331 to fix the centrifugal plate 35.

[0032] In this embodiment, the raised portion 352 is provided with equidistant placement holes 354 around the base 351, and a rubber sleeve 341 is installed in the placement hole 354. A reagent tube 6 containing the microbial solution to be separated is inserted into the rubber sleeve 341.

[0033] It should be noted that, as shown in Figures 4, 5, and 7, the centrifuge plate 35 is designed with an upward-curving structure. This allows the reagent tube 6 to be tilted after being inserted into the storage hole 354. This results in a greater centrifugal force on the reagent tube 6 during rotation, which can shorten the centrifugation time and improve work efficiency. When inserting the reagent tube 6 into the storage hole 354, a rubber sleeve 341 is first fitted on it, mainly to increase the friction between the reagent tube 6 and the storage hole 354 and prevent the reagent tube 6 from detaching from the storage hole 354 during high-speed rotation.

[0034] In this embodiment, the inner ring of the bearing 34 is fastened to the rod body of the transmission rod 33, and a rubber washer 341 is fitted in the gap between the outer ring of the bearing 34 and the circular hole 131.

[0035] In this embodiment, the limiting hole 121 is fitted onto the side of the motor 31, and the upper part of the base plate 11 and the lower part of the top plate 13 are provided with screw posts 15. After the screw posts 15 of the left outer shell 1 and the right outer shell 2 are assembled, the screw posts 15 are aligned and connected, and screws are screwed in to fix the left outer shell 1 and the right outer shell 2 together.

[0036] In this embodiment, a limiting wheel 4 is provided between the limiting plate 121 and the top plate 13. A limiting ring 41 is provided at the center of the limiting wheel 4 and is fitted onto the rod body of the transmission rod 33. The outer side of the limiting wheel 4 is a circular ring 42, which abuts against the inner wall of the left outer shell 1 and the right outer shell 2. The inner wall of the left outer shell 1 and the right outer shell 2 is provided with a plurality of limiting blocks 24, which are distributed on the upper and lower sides of the circular ring 42.

[0037] It should be noted that, as shown in Figures 3 and 4, the limiting plate 121 is used to fix the motor 31, and the limiting wheel 4 is also used to hold the transmission rod 33. Since the transmission rod 33 is long, there is a risk of displacement when rotating at high speed. Therefore, the limiting wheel 4 is set to hold the transmission rod 33, so that the transmission rod 33 can only rotate within the limiting ring 41 and will not be displaced.

[0038] In this embodiment, the left outer shell 1 and the right outer shell 2 are assembled and fixed to form a cylindrical structure, and a glass cover 5 is installed on top to seal the entire protruding centrifugal plate 33.

[0039] Referring to Figures 1 to 7, the assembly method of this device is as follows:

[0040] First, remove the left housing 1, fix the motor 31 to the connecting sleeve 32, install the limiting wheel 4 onto the transmission rod 33, then install the bearing 34 onto the upper part of the transmission rod 33, and then fix the rubber washer 341 to the outside of the bearing 34. Next, clamp the motor 31 into half of the motor slot 21, and clamp the side of the motor 31 into the limiting slot 22. Fix the connecting sleeve 32 to the bottom of the transmission rod 33, then clamp the rubber washer 341 into half of the semi-circular groove 23, and then... The ring 42 of the limiting wheel 4 is installed in the middle of the upper and lower limiting blocks 24. Finally, the other half of the right shell 2 can be aligned with the left shell 1 and assembled together. The screws are tightened from the holes of the screw post 15 to make the left shell 1 and the right shell 2 firmly fixed together. Finally, the centrifuge plate 35 is installed on the top of the transmission rod 33, the rubber sleeve 355 is installed, the sealed reagent tube 6 containing the microbial solution is inserted into the rubber sleeve 355, the glass cover 5 is covered, and the battery is installed to start the separation work.

[0041] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A microbial isolation and testing device, characterized in that, include: The left outer shell (1), right outer shell (2), and centrifugal mechanism (3) are arranged in the following order from bottom to top: bottom plate (11), limiting plate (12), and top plate (13). The bottom plate (11) has a semi-circular motor groove (21) at its center, the limiting plate (12) has a semi-circular limiting groove (22) at its center, and the top plate (13) has a semi-circular semi-circular groove (23) at its center. The internal structure of the right outer shell (2) is the same as that of the left outer shell (1). The motor groove (21) is assembled into a circular groove, and the limiting groove (22) and the semi-circular groove (23) are aligned and assembled to form a limiting hole (121) and a circular hole (131) respectively. The interior of the left outer shell (1) is... A battery compartment (14) is provided; the centrifugal mechanism (3) includes a motor (31), a connecting sleeve (32), a transmission rod (33), a bearing (34), a centrifugal plate (35), and a fixing cover (36). The motor (31) is installed in the motor slot (21). The top of the output shaft of the motor (31) is fixed with the connecting sleeve (32). The upper end of the connecting sleeve (32) is vertically installed with the transmission rod (33). The transmission rod (33) passes through the round hole (131) and the bearing (34) is fitted at the round hole (131). The top of the transmission rod (33) is fitted with the centrifugal plate (35). At the same time, the fixing cover (36) is installed on the centrifugal plate (35) to fasten the centrifugal plate (35) to the transmission rod (33).

2. The microbial isolation and testing device according to claim 1, characterized in that, The centrifugal plate (35) has a planar circular base (351) at its center. A raised part (352) extends circumferentially around the edge of the base (351) and bends upward. The raised part (352) and the base (351) form a continuous arc surface. A through hole (353) is provided in the center of the base (351). The top of the transmission rod (33) is provided with a stepped lower truncated cone (332) and an upper truncated cone (331). The centrifugal plate (35) is installed on the lower truncated cone (332) through the through hole (353). The upper truncated cone (331) has a thread on its side. The inner side of the fixing cover (36) has a matching thread. After the centrifugal plate (35) is installed on the lower truncated cone (332), the fixing cover (36) is screwed onto the upper truncated cone (331) to fix the centrifugal plate (35).

3. The microbial isolation and testing device according to claim 2, characterized in that, The raised part (352) is provided with equidistant placement holes (354) around the base (351). A rubber sleeve (355) is installed in the placement hole (354), and a reagent tube (6) containing the microbial solution to be separated is inserted into the rubber sleeve (355).

4. The microbial isolation and testing device according to claim 1, characterized in that, The inner ring of the bearing (34) is fastened to the rod body of the transmission rod (33), and a rubber washer (341) is fitted in the gap between the outer ring of the bearing (34) and the round hole (131).

5. The microbial isolation and testing device according to claim 1, characterized in that, The limiting hole (121) is fitted on the side of the motor (31). The upper part of the base plate (11) and the lower part of the top plate (13) are provided with screw posts (15). After the screw posts (15) of the left outer shell (1) and the right outer shell (2) are assembled, the screw posts (15) are aligned and connected. Screws are screwed in to fix the left outer shell (1) and the right outer shell (2) together.

6. A microbial isolation and testing device according to claim 1 or 5, characterized in that, A limiting wheel (4) is provided between the limiting plate (12) and the top plate (13). A limiting ring (41) is provided at the center of the limiting wheel (4) and is fitted onto the rod of the transmission rod (33). The outer side of the limiting wheel (4) is a circular ring (42) and the circular ring (42) abuts against the inner wall of the left outer shell (1) and the right outer shell (2). The inner wall of the left outer shell (1) and the right outer shell (2) is provided with several limiting blocks (24). The limiting blocks (24) are distributed on the upper and lower sides of the circular ring (42).

7. The microbial isolation and testing device according to claim 1, characterized in that, The left outer shell (1) and the right outer shell (2) are assembled and fixed to form a cylindrical structure, and a glass cover (5) is installed on the top to seal the entire protruding centrifugal plate (35).

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