Production device of glucosyl stevioside

Abstract

The utility model discloses a glucosyl stevioside production device, and relates to the technical field of glucosyl stevioside production, an inlet of a conversion tank is communicated with a raw material tank, a cyclodextrin tank, a beta cyclodextrin transferase tank and a first alkali liquor tank, and an outlet of the conversion tank is communicated with a reaction tank; an inlet of the reaction tank is respectively communicated with a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a UGT glycosyltransferase tank and a second alkali liquor tank through pipelines, an outlet of the reaction tank is communicated with a cation exchange resin column, an inlet of the cation exchange resin column is communicated with an ethanol tank, an outlet of the cation exchange resin column is communicated with a concentration tank, and the concentration tank is communicated with a second alkali liquor tank. An outlet of the concentration tank is communicated with a dryer, and an outlet of the dryer is communicated with a glucosyl stevioside tank. The residue of unreacted glucoside in the glucosyl stevioside is reduced through a double-enzyme method, the conversion rate of a substrate is improved, the residue of the substrate is further reduced, and the taste of the product is integrally improved.

Description

A production apparatus for glucosylstevioside Technical Field

[0001] This utility model relates to the field of glucosylstevioside production technology, specifically to a glucosylstevioside production apparatus. Background Technology

[0002] Steviol glycosides are sweet compounds extracted from stevia leaves and are widely used as food sweeteners. However, steviol glycosides have a certain bitterness, which may be unappealing to some consumers. Therefore, researchers began to explore ways to reduce the bitterness of steviol glycosides and improve their sweetness. Glucosyl steviol glycosides are obtained by enzymatically glucosylating steviol glycosides extracted from stevia leaves, followed by evaporation, concentration, and spray drying. The enzymatic treatment of steviol glycosides significantly improves undesirable flavors such as bitterness.

[0003] Currently, glucosylsteviosides obtained by glucosylation of rebaudioside A and steviol glycosides via cyclodextrin transferase have an unreacted glycoside content greater than 4%. The first method, increasing the amount of dextrin added, while increasing substrate conversion and reducing unreacted glycosides to some extent, requires a large amount of dextrin and is difficult to reduce unreacted glycosides to below 4%. Excessive dextrin also reduces the product's sweetness and mouthfeel. Another method, using compound steviol glycosides, can dilute unreacted glycosides, but this involves large amounts of other steviol glycosides, significantly altering the product's properties and resulting in substandard total glycoside content and other indicators, failing to meet quality standards. The third method involves resin separation, but this method suffers from low yield, increased waste, and a high degree of glycosylation, also significantly altering the product's properties and limiting the application of glucosylstevioside products using these raw materials as substrates. Summary of the Invention

[0004] The technical problem to be solved by this utility model is to provide a production device for glucosyl steviol glycosides, which increases the conversion rate of the substrate and improves the taste of the product, in order to address the shortcomings of the existing technology.

[0005] To solve the above-mentioned technical problems, the technical solution of this utility model is as follows:

[0006] A production apparatus for glucosylstevioside includes a conversion tank. The inlet of the conversion tank is connected via pipes to a raw material tank, a cyclodextrin tank, a beta-cyclodextrin transferase tank, and a first alkali tank. The outlet of the conversion tank is connected via pipes to a reaction tank. The inlet of the reaction tank is connected via pipes to a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a UGT glycosyltransferase tank, and a second alkali tank. The outlet of the reaction tank is connected via pipes to a cation exchange resin column. The inlet of the cation exchange resin column is connected via pipes to an ethanol tank. The outlet of the cation exchange resin column is connected via pipes to a concentration tank. The lower inlet of the concentration tank is connected via pipes to a dryer. The outlet of the dryer is connected to a glucosylstevioside tank.

[0007] As an improved technical solution, the top gas phase outlet of the concentration tank is connected to an ethanol recovery tank via a pipeline.

[0008] As an improved technical solution, the outlet of the ethanol recovery tank is connected to the inlet of the ethanol tank via a pipeline.

[0009] As an improved technical solution, the inlet of the cation exchange resin column is connected to a purified water tank via a pipeline, the outlet of the cation exchange resin column is connected to a rinsing water tank via a pipeline, and the outlet of the rinsing water tank is connected to the inlet of the conversion tank via a pipeline.

[0010] As an improved technical solution, the outlet of the cation exchange resin column is connected to a water-top alcohol tank via a pipeline, and the outlet of the water-top alcohol tank is connected to the inlet of the concentration tank via a pipeline.

[0011] As an improved technical solution, the conversion tank is equipped with a first online pH sensor, and the outlet of the first alkali tank is equipped with a first automatic shut-off valve. The first online pH sensor and the first automatic shut-off valve are interlocked to the control system.

[0012] As an improved technical solution, the reaction vessel is equipped with a second online pH sensor, and the outlet of the second alkali tank is equipped with a second automatic shut-off valve. The second online pH sensor and the second automatic shut-off valve are interlocked to the control system.

[0013] Due to the adoption of the above technical solution, the beneficial effects of this utility model are:

[0014] This invention relates to a production apparatus for glucosylstevioside, comprising a conversion tank. The inlet of the conversion tank is connected via pipes to a raw material tank, a cyclodextrin tank, a beta-cyclodextrin transferase tank, and a first alkali tank. The outlet of the conversion tank is connected via pipes to a reaction tank. The inlet of the reaction tank is connected via pipes to a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a UGT glycosyltransferase tank, and a second alkali tank. The outlet of the reaction tank is connected via pipes to a cation exchange resin column. The inlet of the cation exchange resin column is connected via pipes to an ethanol tank. The outlet of the cation exchange resin column is connected via pipes to a concentration tank. The lower inlet of the concentration tank is connected via pipes to a dryer. The outlet of the dryer is connected to a glucosylstevioside tank. Using rebaudioside A or steviol as raw materials and cyclodextrin as an excipient, beta-cyclodextrin transferase was added for glycosylation to obtain a glucosylstevioside solution I with rebaudioside A or steviol as the substrate. Then, sucrose, sucrose synthase, uridine diphosphate, and UGT glycosyltransferase were added to further convert unreacted rebaudioside A or steviol into rebaudioside D or rebaudioside E, yielding a glucosylstevioside solution II. This solution was then adsorbed onto a cation exchange resin column to remove residual dextrin and sucrose. Ethanol elution yielded a purified glucosylstevioside solution III. Finally, after dealcoholization and drying, the glucosylstevioside product was obtained. This dual-enzyme method reduced the residue of unreacted glycosides in glucosylstevioside, improved substrate conversion, further reduced substrate residue, and improved the overall taste of the product.

[0015] The concentration tank of this invention has a gas phase outlet at the top connected to an ethanol recovery tank via a pipeline. The ethanol is evaporated by heating, thus avoiding the possibility of ethanol residue affecting the taste of the product.

[0016] The outlet of the ethanol recovery tank is connected to the inlet of the ethanol tank via a pipeline. The recovered ethanol is reused for product analysis, avoiding waste of raw materials, saving costs, and being environmentally friendly.

[0017] The inlet of the cation exchange resin column is connected to a purified water tank via a pipeline, and the outlet of the cation exchange resin column is connected to a rinsing water tank via a pipeline. The outlet of the rinsing water tank is connected to the inlet of the conversion tank via a pipeline. By rinsing the adsorbed cation exchange resin column, the resulting dextrin and sucrose solution can be reused for conversion, reducing the waste of raw materials.

[0018] The outlet of the cation exchange resin column is connected to a water-top alcohol tank via a pipeline, and the outlet of the water-top alcohol tank is connected to the inlet of the concentration tank via a pipeline. By returning the water-top alcohol to the concentration tank for concentration and drying, the product yield is improved and the waste of raw materials is reduced.

[0019] The conversion tank is equipped with a first online pH sensor, and the outlet of the first alkali tank is equipped with a first automatic shut-off valve. The first online pH sensor and the first automatic shut-off valve are interlocked to the control system. The reaction tank is equipped with a second online pH sensor, and the outlet of the second alkali tank is equipped with a second automatic shut-off valve. The second online pH sensor and the second automatic shut-off valve are interlocked to the control system. The control system adjusts the opening and closing of the first and second automatic shut-off valves according to the values ​​of the first and second online pH sensors to ensure that the pH value of the materials in the conversion tank and the reaction tank is at a stable value, which is conducive to the reaction. Attached Figure Description

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Figure 1 is a structural schematic diagram of an embodiment of the present utility model;

[0022] The tank consists of: 1. Conversion tank; 2. Raw material tank; 3. Cyclodextrin tank; 4. Beta-cyclodextrin transferase tank; 5. First alkali tank; 6. Reaction tank; 7. UGT glycosyltransferase tank; 8. Second alkali tank; 9. Cation exchange resin column; 10. Ethanol tank; 11. Concentration tank; 12. Dryer; 13. Glucosylstevioside tank; 14. Ethanol recovery tank; 15. Purified water tank; 16. Rinse water tank; 17. Water-top alcohol tank; 18. First online pH sensor; 19. First automatic shut-off valve; 20. Second online pH sensor; 21. Second automatic shut-off valve; 22. Sucrose tank; 23. Sucrose synthase tank; 24. Urate diphosphate tank. Detailed Implementation

[0023] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0024] As shown in Figure 1, a production apparatus for glucosylstevioside includes a conversion tank 1. The inlet of the conversion tank 1 is connected to a raw material tank 2, a cyclodextrin tank 3, a beta-cyclodextrin transferase tank 4, and a first alkali tank 5 via pipelines. The outlet of the conversion tank 1 is connected to a reaction tank 6 via pipelines. The inlet of the reaction tank 6 is connected to a sucrose tank 22, a sucrose synthase tank 23, a uridine diphosphate tank 24, a UGT glycosyltransferase tank 7, and a second alkali tank 8 via pipelines. The outlet of the reaction tank 6 is connected to a cation exchange resin column 9 via pipelines. The inlet of the cation exchange resin column 9 is connected to an ethanol tank 10 via pipelines. The outlet of the cation exchange resin column 9 is connected to a concentration tank 11 via pipelines. The lower inlet of the concentration tank 11 is connected to a dryer 12 via pipelines. The outlet of the dryer 12 is connected to a glucosylstevioside tank 13. Using rebaudioside A or steviol as raw materials and cyclodextrin as an excipient, beta-cyclodextrin transferase was added to carry out a glycosylation reaction to obtain a glucosylstevioside solution I with rebaudioside A or steviol as substrate. Then, sucrose, sucrose synthase, uridine diphosphate and UGT glycosyltransferase (UGT glycosyltransferase is derived from the glycosyltransferase UGTSL2 mutant UGTSL2_M in patent CN119220516A, and sucrose synthase is derived from the sucrose synthase AtSuSy in Chinese patent CN119220516A) were added to further convert the unreacted rebaudioside A or steviol in the solution into rebaudioside D or rebaudioside E to obtain a glucosylstevioside solution II. Then, the glucosylstevioside was adsorbed through a cation exchange resin column 9 to remove residual dextrin and sucrose. Ethanol elution was used to obtain a purified glucosylstevioside solution III. Finally, after deethanolination and drying, the glucosylstevioside product was obtained. By reducing the residue of unreacted glycosides in glucosylstevioside using a dual-enzyme method, the conversion rate of the substrate is improved, further reducing substrate residue and enhancing the overall taste of the product.

[0025] The top gas phase outlet of the concentration tank 11 is connected to the ethanol recovery tank 14 via a pipeline. The ethanol is evaporated by heating, avoiding the possibility of ethanol residue affecting the taste of the product.

[0026] The outlet of the ethanol recovery tank 14 is connected to the inlet of the ethanol tank 10 via a pipeline. The recovered ethanol is reused for product analysis, avoiding waste of raw materials, saving costs, and being environmentally friendly.

[0027] The inlet of the cation exchange resin column 9 is connected to a purified water tank 15 via a pipe, and the outlet of the cation exchange resin column 9 is connected to a rinsing water tank 16 via a pipe. The outlet of the rinsing water tank 16 is connected to the inlet of the conversion tank 1 via a pipe. By rinsing the adsorbed cation exchange resin column 9, the resulting dextrin and sucrose solution can be reused for conversion, reducing the waste of raw materials.

[0028] The outlet of the cation exchange resin column 9 is connected to a water-top alcohol tank 17 via a pipeline. The outlet of the water-top alcohol tank 17 is connected to the inlet of the concentration tank 11 via a pipeline. By returning the water-top alcohol to the concentration tank 11 for concentration and drying, the product yield is improved and the waste of raw materials is reduced.

[0029] The conversion tank 1 is equipped with a first online pH sensor 18, and the outlet of the first alkali tank 5 is equipped with a first automatic shut-off valve 19. The first online pH sensor 18 and the first automatic shut-off valve 19 are interlocked to the control system. The reaction tank 6 is equipped with a second online pH sensor 20, and the outlet of the second alkali tank 8 is equipped with a second automatic shut-off valve 21. The second online pH sensor 20 and the second automatic shut-off valve 21 are interlocked to the control system. The control system adjusts the opening and closing of the first automatic shut-off valve 19 and the second automatic shut-off valve 21 according to the values ​​of the first online pH sensor 18 and the second online pH sensor 20 to ensure that the pH value of the materials in the conversion tank 1 and the reaction tank 6 is at a stable value, which is conducive to the reaction.

[0030] It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the invention, and these equivalent forms also fall within the scope defined by the appended claims.

Claims

1. A production apparatus for glucosylstevioside, characterized in that: The system includes a conversion tank, the inlet of which is connected via pipes to a raw material tank, a cyclodextrin tank, a beta-cyclodextrin transferase tank, and a first alkali tank. The outlet of the conversion tank is connected via pipes to a reaction tank, the inlet of which is connected via pipes to a sucrose tank, a sucrose synthase tank, a uridine diphosphate tank, a UGT glycosyltransferase tank, and a second alkali tank. The outlet of the reaction tank is connected via pipes to a cation exchange resin column, the inlet of which is connected via pipes to an ethanol tank, and the outlet of which is connected via pipes to a concentration tank. The lower inlet of the concentration tank is connected via pipes to a dryer, and the outlet of the dryer is connected to a glucosylstevioside tank.

2. The apparatus for producing glucosyl steviol glycosides as described in claim 1, characterized in that: The top gas phase outlet of the concentration tank is connected to an ethanol recovery tank via a pipeline.

3. The apparatus for producing glucosyl steviol glycosides as described in claim 2, characterized in that: The outlet of the ethanol recovery tank is connected to the inlet of the ethanol tank via a pipeline.

4. The apparatus for producing glucosyl steviol glycosides as described in claim 1, characterized in that: The inlet of the cation exchange resin column is connected to a purified water tank via a pipe, and the outlet of the cation exchange resin column is connected to a rinsing water tank via a pipe. The outlet of the rinsing water tank is connected to the inlet of the conversion tank via a pipe.

5. The apparatus for producing glucosyl steviol glycosides as described in claim 4, characterized in that: The outlet of the cation exchange resin column is connected to a water-top alcohol tank via a pipeline, and the outlet of the water-top alcohol tank is connected to the inlet of the concentration tank via a pipeline.

6. The apparatus for producing glucosyl steviol glycosides as described in claim 1, characterized in that: The conversion tank is equipped with a first online pH sensor, and the outlet of the first alkali tank is equipped with a first automatic shut-off valve. The first online pH sensor and the first automatic shut-off valve are interlocked to the control system.

7. The apparatus for producing glucosyl steviol glycosides as described in claim 1, characterized in that: The reaction vessel is equipped with a second online pH sensor, and the outlet of the second alkali tank is equipped with a second automatic shut-off valve. The second online pH sensor and the second automatic shut-off valve are interlocked to the control system.

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