Efficient purification device based on QuEChERS method

Abstract

The utility model relates to an efficient purification device based on a QuEChERS method, and belongs to the technical field of sample pretreatment, the efficient purification device comprises a purification pipe filled with an adsorbent, the bottom of the purification pipe is provided with a microfiltration membrane, and the purification pipe is detachably provided with a piston rod. The method has the advantage of improving the sample purification efficiency.

Description

Technical Field

[0001] This application relates to the field of sample pretreatment technology, and in particular to a high-efficiency purification device based on the QuEChERS method. Background Technology

[0002] The QuEChERS method is a widely used sample pretreatment method in agricultural products and traditional Chinese medicine. Its name is an abbreviation for Quick, Easy, Cheap, Effective, Rugged, and Safe, signifying its rapid, simple, economical, efficient, durable, and safe nature. It is widely used for the determination of various pesticide and metabolite residues in plant-derived foods.

[0003] The QuEChERS method utilizes the interaction between the adsorbent packing material and impurities in the sample matrix to achieve purification by adsorbing impurities. After extraction with acetonitrile (or acidified acetonitrile), the sample undergoes salting-out separation using extraction salts. Then, leveraging the matrix dispersion extraction mechanism, PSA or other adsorbents bind to most interfering substances in the matrix, which are then removed by centrifugation to achieve purification.

[0004] The specific steps include: Extraction: Weigh the sample and add it to a centrifuge tube, then add acetonitrile and shake vigorously for 1 minute, followed by centrifugation at 4200 rpm for 5 minutes. Purification: Transfer the supernatant from the centrifuge tube to another centrifuge tube, add adsorbents such as PSA, vortex to mix for 1 minute, and then centrifuge at 4200 rpm for 5 minutes. The current purification process is rather cumbersome and has low efficiency, therefore it needs improvement. Utility Model Content

[0005] To improve sample purification efficiency, this application provides a high-efficiency purification device based on the QuEChERS method.

[0006] The present application provides a high-efficiency purification device based on the QuEChERS method, which adopts the following technical solution: A high-efficiency purification device based on the QuEChERS method includes a purification tube filled with adsorbent, a microporous filter membrane installed at the bottom of the purification tube, and a piston rod detachably installed on the purification tube.

[0007] By adopting the above technical solution, after the sample is centrifuged in a centrifuge tube, the supernatant is drawn into a purification tube. Then, the piston rod is inserted into the purification tube, and the extracted supernatant is passed through the adsorbent and microporous filter membrane in the purification tube to adsorb impurities, thereby purifying the extract. Compared with the existing method of passing the sample through another centrifuge tube for oscillation and centrifugation, the operation efficiency is improved, thereby improving the purification efficiency of the sample.

[0008] Preferably, it also includes a base, a frame, a support rod, and a placement tray. The frame and the support rod are installed on the base at intervals. The placement tray is rotatably connected to the upper end of the support rod. Multiple purification tubes are provided and are placed on the placement tray at intervals along the circumferential direction. The piston rod can be located directly above one of the purification tubes. The frame is provided with a drive assembly that drives the piston rod to move down and insert into the purification tube to push the sample in the purification tube to flow out.

[0009] By adopting the above technical solution, the sample supernatant extracted from the centrifuge tube is transferred to one of the purification tubes in the placement tray. Then, the placement tray is rotated so that the purification tube containing the supernatant is in the position of the piston rod. The piston rod is driven down by the drive assembly, thereby pushing the supernatant out of the purification tube. In this process, the used purification tube can be replaced, or the adsorbent can be filled and replaced in the purification tube. Alternatively, the same or different sample liquids can be purified sequentially through multiple purification tubes, thereby improving the purification efficiency.

[0010] Preferably, the drive assembly includes a mounting rod, a moving block, a lead screw, and a motor. The lead screw is vertically rotatably connected to the frame, the moving block is vertically slidably connected to the frame and threadedly connected to the lead screw, one end of the mounting rod is fixedly connected to the side wall of the moving block, the motor is installed in the base, and the output shaft of the motor is coaxially fixedly connected to the lead screw, and the piston rod is fixed on the mounting rod.

[0011] By adopting the above technical solution, the motor drives the lead screw to rotate, thereby causing the moving block to slide down the frame, which in turn causes the piston rod fixed on the mounting rod to move up and down, thus automatically realizing the movement of the piston rod.

[0012] Preferably, the placement tray is provided with a plurality of receiving holes along the circumferential direction for inserting and placing the purification tube, and the placement tray is provided with a plurality of arc-shaped pressing blocks located on the wall of the receiving holes. The placement tray is provided with an adjustment mechanism that synchronously drives the plurality of arc-shaped pressing blocks to press and fix the purification tube or move it away from the purification tube.

[0013] By adopting the above technical solution, the adjustment mechanism can make the arc-shaped pressing block press and fix the purification tube in the receiving hole, thereby improving the stability of the purification tube in the placement tray, and making it easier for the piston rod to be inserted into or removed from the purification tube.

[0014] Preferably, the adjusting mechanism includes a cam, a fixed rod, multiple abutting rods, multiple springs, multiple guide sleeves, and multiple abutting blocks. The fixed rod is coaxially keyed to the cam so that the fixed rod can drive the cam to rotate synchronously, and the fixed rod can move along the cam axis. The bottom of the placement tray is provided with a fixing groove for inserting and fixing the fixed rod. The guide sleeve is fixedly disposed at the bottom of the placement tray between the arc-shaped pressing block and the cam. The abutting rod is movably inserted into the guide sleeve. One end of the abutting rod is fixedly connected to the arc-shaped pressing block. The abutting block is fixedly connected to the end of the abutting rod away from the arc-shaped pressing block. The spring is sleeved on the abutting rod, with one end of the spring abutting the guide sleeve and the other end abutting the abutting block.

[0015] By adopting the above technical solution, the fixed rod is rotated to drive the cam to rotate, so that the wheel surface of the cam abuts against the abutting rod, and the abutting rod pushes the arc-shaped pressing block to press against the purification tube. Then, the fixed rod is moved down and inserted into the fixed groove, so that the cam is fixed, and the arc-shaped pressing block presses against the fixed purification tube. When the purification tube needs to be replaced, simply lift the fixed rod up and then rotate it in the opposite direction.

[0016] Preferably, a rotary button with a diameter larger than that of the fixed rod is fixedly installed on the upper end of the placement plate extending from the fixed rod.

[0017] By adopting the above technical solution, the fixed rod can be easily operated by rotating the button.

[0018] Preferably, the upper end of the support rod is provided with a support plate, the placement plate is rotatably connected to the upper surface of the support plate, the upper surface of the support plate is embedded with a first magnet, and the lower surface of the placement plate is embedded with a plurality of second magnets that can attract each other to the first magnet at the positions corresponding to the receiving holes.

[0019] By adopting the above technical solution, when the first magnet and the second magnet are attracted during the manual rotation of the placement tray, it indicates that the purification tube containing the sample liquid is located directly below the piston rod, thereby improving the convenience and accuracy of the operation.

[0020] Preferably, the base has a recessed opening on one side for placing the collection bottle.

[0021] By adopting the above technical solution, the clearance opening on one side of the base facilitates the replacement of the collection bottle.

[0022] In summary, this application includes at least one of the following beneficial technical effects:

[0023] 1. After the sample is centrifuged in a centrifuge tube, the supernatant is drawn into a purification tube. Then, the piston rod is inserted into the purification tube to allow the extracted supernatant to pass through the adsorbent and microporous filter membrane in the purification tube to adsorb impurities, thereby purifying the extract. Compared with the existing method of passing the sample through another centrifuge tube for oscillation and centrifugation, this method improves the operational efficiency and thus improves the purification efficiency of the sample.

[0024] 2. The supernatant extracted from the centrifuge tube is transferred to one of the purification tubes in the placement tray. Then, the placement tray is rotated so that the purification tube containing the supernatant is in the position of the piston rod. The piston rod is driven down by the drive assembly, thereby pushing the supernatant out of the purification tube. In this process, the used purification tube can be replaced, the adsorbent can be filled and replaced, or multiple purification tubes can be used to purify the same or different sample liquids in sequence, thereby improving the purification efficiency. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of the purification tube in the embodiments of this application.

[0026] Figure 2 This is a side view of the purification device in an embodiment of this application.

[0027] Figure 3 This is a top view of the internal structure of the disk in an embodiment of this application.

[0028] Figure 4 This is a top view of the bottom of the tray in an embodiment of this application.

[0029] Explanation of reference numerals in the attached drawings: 1. Purification tube; 2. Microporous filter membrane; 3. Piston rod; 4. Base; 41. Frame; 42. Support rod; 43. Clearance opening; 5. Placement tray; 51. Receiving hole; 52. Fixing groove; 53. Support tray; 54. First magnet; 55. Second magnet; 6. Drive assembly; 61. Mounting rod; 611. Mounting hole; 612. Abutting screw; 62. Moving block; 63. Lead screw; 64. Motor; 7. Arc-shaped pressing block; 8. Adjustment mechanism; 81. Cam; 82. Fixing rod; 83. Abutting rod; 84. Spring; 85. Guide sleeve; 86. Abutting block; 87. Rotary button. Detailed Implementation

[0030] The following is in conjunction with the appendix Figure 1-4 This application will be described in further detail.

[0031] This application discloses a high-efficiency purification device based on the QuEChERS method. (Refer to...) Figure 1The purification device includes a purification tube 1, which is a syringe tube. A microporous filter membrane 2 is installed at the bottom of the purification tube 1. The purification tube 1, which is connected to the microporous filter membrane 2, is filled with an adsorbent such as PSA. A piston rod 3 is detachably installed on the purification tube 1. In the first step of the QuEChERS method, the sample is centrifuged in a centrifuge tube, and the supernatant is drawn into the purification tube 1. Then, the piston rod 3 is inserted into the purification tube 1, and the extracted supernatant is passed through the adsorbent and the microporous filter membrane 2 in the purification tube 1 to adsorb impurities, thereby purifying the extract. Compared with the existing traditional method, which involves adding adsorbent to another centrifuge tube, followed by shaking and centrifugation to obtain a purified supernatant, direct filtration through the adsorbent in the purification tube 1 improves the purification efficiency.

[0032] Reference Figure 2 To improve purification efficiency and ease of operation, the purification device also includes a base 4, a frame 41, a support rod 42, a placement tray 5, and a drive assembly 6. The frame 41 is fixedly installed on one side of the base 4, the support rod 42 is fixedly installed on the other side of the base 4, and the placement tray 5 is rotatably installed on the upper end of the support rod 42. The placement tray 5 can place multiple purification tubes 1 in a circumferential direction, and the multiple purification tubes 1 can rotate and be positioned directly below the piston rod 3. The drive assembly 6 can drive the piston rod 3 to move down and insert into the purification tube 1 located directly below, so as to push the sample in the purification tube 1 out for purification. A clearance port 43 for placing a collection bottle is provided on one side of the base 4. The supernatant extracted from the centrifuge tube is transferred to one of the purification tubes 1 in the placement tray 5. Then, the placement tray 5 is rotated so that the purification tube 1 with supernatant is in the position of the piston rod 3. The piston rod 3 is driven down by the drive assembly 6, thereby pushing the supernatant out of the purification tube 1 and collecting it in the collection bottle below. Placing multiple purification tubes 1 in the placement tray 5 allows for the replacement of used purification tubes 1, the filling and replacement of adsorbent in the purification tubes 1, or the sequential purification of the same or different sample liquids through multiple purification tubes 1, thereby improving purification efficiency.

[0033] Specifically, the drive assembly 6 includes a mounting rod 61, a moving block 62, a lead screw 63, and a motor 64. The lead screw 63 is vertically rotatably connected to the frame 41, and the moving block 62 is vertically slidably connected to the frame 41 and threadedly connected to the lead screw 63. One end of the mounting rod 61 is fixedly connected to the side wall of the moving block 62. The motor 64 is mounted in the base 4, and the output shaft of the motor 64 is coaxially fixedly connected to the lead screw 63. The piston rod 3 is fixed to the mounting rod 61. The motor 64 drives the lead screw 63 to rotate, thereby causing the moving block 62 to slide up and down the frame 41, thereby causing the piston rod 3 fixed on the mounting rod 61 to move up and down, thus automatically realizing the movement of the piston rod 3. Specifically, one end of the mounting rod 61 has a downward-facing mounting hole 611, and the piston rod 3 is inserted into the mounting hole 611. One end of the mounting rod 61 is threadedly connected to an abutting screw 612 that abuts against the side wall of the piston rod 3.

[0034] Reference Figure 2 and Figure 3 The placement tray 5 has multiple receiving holes 51 arranged along its circumference for inserting and placing the purification tube 1. Multiple arc-shaped pressing blocks 7 are located on the walls of the receiving holes 51 within the placement tray 5. The placement tray 5 is equipped with an adjustment mechanism 8 that synchronously drives the multiple arc-shaped pressing blocks 7 to press and fix the purification tube 1 or move it away from the purification tube 1. The adjustment mechanism 8 allows the arc-shaped pressing blocks 7 to press and fix the purification tube 1 within the receiving holes 51, thereby improving the stability of the purification tube 1 within the placement tray 5, thus facilitating the insertion or removal of the piston rod 3 from the purification tube 1. At least two receiving holes 51 are provided; in this embodiment, four receiving holes 51 are provided.

[0035] Reference Figure 2 and Figure 3 Furthermore, to facilitate the placement of the purification tube 1 directly below the piston rod 3, a support plate 53 is provided at the upper end of the support rod 42. The placement plate 5 is rotatably connected to the upper surface of the support plate 53. A first magnet 54 is embedded in the upper surface of the support plate 53, and the first magnet 54 is on the same plane as the piston rod 3. Multiple second magnets 55, which can attract each other with the first magnet 54, are embedded in the lower surface of the placement plate 5 at positions corresponding to the receiving holes 51. During the manual rotation of the placement plate 5, after the first magnet 54 and the second magnets 55 attract each other, the purification tube 1 is located directly below the piston rod 3, thereby improving the convenience and accuracy of operation.

[0036] Reference Figure 2 and Figure 3 The adjusting mechanism 8 includes a cam 81, a fixed rod 82, multiple abutting rods 83, multiple springs 84, multiple guide sleeves 85, and multiple abutting blocks 86. The fixed rod 82 is coaxially keyed to the cam 81, so that the fixed rod 82 can drive the cam 81 to rotate synchronously, and the fixed rod 82 can move axially along the cam 81. Figure 4The bottom of the placement tray 5 is provided with a fixing groove 52 for insertion and fixing of the fixing rod 82. In this embodiment, the fixing rod 82 is provided with vertical teeth along the circumferential direction. The center of the cam 81 and the inner wall of the fixing groove 52 are provided with toothed grooves along the circumferential direction that cooperate with the teeth of the fixing rod 82. The teeth of the fixing rod 82 and the toothed grooves at the center of the cam 81 are connected by a key, thereby achieving synchronous rotation. The fixing rod 82 can be inserted into the fixing groove 52, thereby fixing the cam 81 so that it cannot rotate. The guide sleeve 85 is fixedly set in the bottom of the placement tray 5 between the arc-shaped pressing block 7 and the cam 81. The abutment rod 83 is movably inserted into the guide sleeve 85. One end of the abutment rod 83 is fixedly connected to the arc-shaped pressing block 7, and the abutment block 86 is fixedly connected to the end of the abutment rod 83 away from the arc-shaped pressing block 7. The spring 84 is sleeved on the abutment rod 83. One end of the spring 84 abuts against the guide sleeve 85, and the other end abuts against the abutment block 86. To facilitate operation of the fixing rod 82, a rotary button 87 with a diameter larger than that of the fixing rod 82 is fixedly installed on the upper end of the placement plate 5. By rotating the button 87, the cam 81 is driven to rotate, so that the wheel surface of the cam 81 abuts against the abutting rod 83. The abutting rod 83 pushes the arc-shaped pressing block 7 to press against the purification tube 1. Then, the fixing rod 82 is moved down and inserted into the fixing groove 52, thereby fixing the cam 81 and causing the arc-shaped pressing block 7 to press against and fix the purification tube 1. When it is necessary to replace the purification tube 1, simply lift the fixing rod 82 and then rotate it in the opposite direction.

[0037] The implementation principle of a high-efficiency purification device based on the QuEChERS method in this application embodiment is as follows: In the first step of the QuEChERS method, after the sample is centrifuged by shaking in a centrifuge tube, the supernatant is drawn into the purification tube 1. Then, the placement plate 5 is rotated so that the purification tube 1 with supernatant is below the piston rod 3. The piston rod 3 is driven to move down by the driving component 6, thereby pushing the supernatant in the purification tube 1 out of the purification tube 1 and collecting it in the collection bottle, thereby completing the purification of the sample and improving the sample purification efficiency.

[0038] Unless otherwise defined, the technical or scientific terms used in this application shall have the ordinary meaning understood by one of ordinary skill in the art to which this application pertains. The terms "first," "second," "third," and similar terms used in this application specification and claims do not indicate any order, quantity, or importance, but are merely used to distinguish different components. The terms "an" or "a" and similar terms do not indicate a quantity limitation, but rather indicate the presence of at least one. The terms "comprising" or "including" and similar terms mean that the elements or objects preceding "comprising" or "including" encompass the elements or objects listed following "comprising" or "including" and their equivalents, and do not exclude other elements or objects. "Above," "below," "left," "right," etc., are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0039] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A high-efficiency purification device based on the QuEChERS method, characterized in that: It includes a purification tube (1) filled with adsorbent, a microporous filter membrane (2) installed at the bottom of the purification tube (1), and a piston rod (3) detachably installed on the purification tube (1); It also includes a base (4), a frame (41), a support rod (42), and a placement tray (5). The frame (41) and the support rod (42) are installed on the base (4) at intervals. The placement tray (5) is rotatably connected to the upper end of the support rod (42). Multiple purification tubes (1) are provided. Multiple purification tubes (1) are placed on the placement tray (5) at intervals along the circumferential direction. The piston rod (3) can be located directly above one of the purification tubes (1). The frame (41) is provided with a drive assembly (6) that drives the piston rod (3) to move down and insert into the purification tube (1) to push the sample in the purification tube (1) to flow out. The drive assembly (6) includes a mounting rod (61), a moving block (62), a lead screw (63), and a motor (64). The lead screw (63) is vertically rotatably connected to the frame (41). The moving block (62) is vertically slidably connected to the frame (41) and threadedly connected to the lead screw (63). One end of the mounting rod (61) is fixedly connected to the side wall of the moving block (62). The motor (64) is installed in the base (4), and the output shaft of the motor (64) is coaxially fixedly connected to the lead screw (63). The piston rod (3) is fixed on the mounting rod (61). The placement tray (5) is provided with a plurality of receiving holes (51) for inserting and placing the purification tube (1) along the circumferential direction. The placement tray (5) is provided with a plurality of arc-shaped pressing blocks (7) located on the wall of the receiving hole (51). The placement tray (5) is provided with an adjustment mechanism (8) that synchronously drives the plurality of arc-shaped pressing blocks (7) to press and fix the purification tube (1) or move away from the purification tube (1). The adjusting mechanism (8) includes a cam (81), a fixed rod (82), multiple abutting rods (83), multiple springs (84), multiple guide sleeves (85), and multiple abutting blocks (86). The fixed rod (82) is coaxially keyed to the cam (81) so that the fixed rod (82) can drive the cam (81) to rotate synchronously. At the same time, the fixed rod (82) can move along the axial direction of the cam (81). The bottom of the placement plate (5) is provided with a fixing groove (52) for inserting and fixing the fixed rod (82). The fixed rod (82) is provided with vertical teeth along the circumferential direction. The center of the cam (81) and the fixing groove (52) are connected. The inner wall of the ) has a toothed groove along the circumferential direction that matches the toothed pattern of the fixed rod (82). The guide sleeve (85) is fixedly set in the bottom of the placement plate (5) between the arc-shaped pressing block (7) and the cam (81). The abutting rod (83) is movably inserted into the guide sleeve (85). One end of the abutting rod (83) is fixedly connected to the arc-shaped pressing block (7). The abutting block (86) is fixedly connected to one end of the abutting rod (83) away from the arc-shaped pressing block (7). The spring (84) is sleeved on the abutting rod (83), and one end of the spring (84) abuts against the guide sleeve (85), and the other end abuts against the abutting block (86).

2. The high-efficiency purification device based on the QuEChERS method according to claim 1, characterized in that: The fixed rod (82) extends out of the upper end of the placement plate (5) and a rotary button (87) with a diameter larger than that of the fixed rod (82) is fixedly installed.

3. The high-efficiency purification device based on the QuEChERS method according to claim 1, characterized in that: The upper end of the support rod (42) is provided with a support plate (53), and the placement plate (5) is rotatably connected to the upper surface of the support plate (53). The upper surface of the support plate (53) is embedded with a first magnet (54), and the lower surface of the placement plate (5) is embedded with a plurality of second magnets (55) that can attract each other with the first magnet (54) at the position corresponding to the receiving hole (51).

4. The high-efficiency purification device based on the QuEChERS method according to claim 1, characterized in that: The base (4) has a recess (43) on one side for placing the collection bottle.

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