An anaerobic bacteria culture device

Abstract

This utility model discloses an anaerobic bacteria cultivation device, specifically relating to the field of anaerobic bacteria cultivation technology. It includes a cultivation chamber and a reaction tank. The cultivation chamber houses the anaerobic bacteria cultivation device, and the reaction tank generates CO2 gas. The cultivation chamber includes a cylindrical body and a top cover. The cylindrical body is open at both ends, and the top cover is detachably and sealed to one end of the cylindrical body. The other end of the cylindrical body is connected to the gas outlet of the reaction tank. The top cover has a gas outlet pipe with a first valve installed on it. This utility model has a simple structure, low cost, and is suitable for small-scale constant-temperature cultivation equipment, promoting the research progress of anaerobic microorganisms.

Description

Technical Field

[0001] This utility model relates to the field of anaerobic bacteria cultivation technology, and in particular to an anaerobic bacteria cultivation device. Background Technology

[0002] Anaerobic culture refers to the technique of culturing microorganisms in an environment completely isolated from molecular oxygen. It is mainly applicable to facultative and obligate anaerobes. These microorganisms are extremely sensitive to oxygen; their growth and reproduction are significantly inhibited, and they may even die, under aerobic conditions. Therefore, when conducting anaerobic culture, a closed environment must be constructed, and oxygen in the environment must be completely removed through physical, chemical, or biological means.

[0003] Currently, conventional anaerobic culture equipment is generally expensive. Coupled with high consumable consumption, strict operational requirements, and persistently high operating and maintenance costs, these factors significantly restrict the large-scale cultivation and in-depth research of anaerobic bacteria in grassroots laboratories. Therefore, there is an urgent market demand for an anaerobic device that is simple in structure, low in cost, and compatible with commonly used small-scale constant-temperature culture equipment in grassroots laboratories. Utility Model Content

[0004] The purpose of this invention is to provide an anaerobic bacteria culture device to solve the problems existing in the prior art. It has a simple structure, low cost, and is suitable for small-scale constant temperature culture equipment, thereby promoting the research progress of anaerobic microorganisms.

[0005] To achieve the above objectives, this utility model provides the following solution:

[0006] This utility model provides an anaerobic bacteria cultivation device, including a cultivation chamber and a reaction tank. The cultivation chamber is used to place the anaerobic bacteria cultivation device, and the reaction tank is used to generate CO2 gas. The cultivation chamber includes a cylinder and a top cover. The cylinder is open at both ends. The top cover is detachably and sealed to one end opening of the cylinder. The other end opening of the cylinder is connected to the gas outlet of the reaction tank. The top cover has a gas outlet pipe, and a first valve is provided on the gas outlet pipe.

[0007] Preferably, it also includes an anaerobic indicator, which is disposed in the culture chamber.

[0008] Preferably, the reaction tank is fixedly installed at the bottom of the cylinder, and the side wall of the reaction tank is provided with an inlet and an outlet. The inlet is connected to and communicates with an inlet pipe, and the outlet is connected to and communicates with an outlet pipe. A second valve is provided on the inlet pipe, and a sealing cap is connected to the end of the outlet pipe.

[0009] Preferably, both the first valve and the second valve are ball valves.

[0010] Preferably, the air outlet pipe, the liquid inlet pipe, and the liquid outlet pipe are made of polytetrafluoroethylene.

[0011] Preferably, the bottom surface of the reaction tank is an inclined bottom surface, and the liquid outlet pipe is provided on the side wall connected to the lowest end of the inclined bottom surface.

[0012] Preferably, the bottom surface of the liquid outlet pipe is flush with the bottom surface of the reaction tank.

[0013] Preferably, it also includes a sealing ring, wherein an annular groove is formed on the outer side wall of the top cover, the sealing ring is engaged in the annular groove, the top cover is inserted into the cylinder, and the sealing ring can seal the annular gap between the outer side wall of the top cover and the inner side wall of the cylinder.

[0014] Preferably, both the culture chamber and the reaction tank are made of transparent material.

[0015] Preferably, the culture chamber and the reaction tank are made of polytetrafluoroethylene.

[0016] The present invention achieves the following technical advantages over the prior art:

[0017] This invention provides an anaerobic bacteria cultivation device, comprising a connected cultivation chamber and a reaction tank. By adding specific chemical reagents to the reaction tank, a chemical reaction occurs, generating CO2 gas. The CO2 gas continuously enters the cultivation chamber, and using the principle of gas replacement, the air in the cultivation chamber is gradually expelled, thereby forming a stable anaerobic environment. The anaerobic bacteria cultivation device also has significant advantages such as simple structure and low cost, and it is reusable, effectively reducing long-term operating costs. It can achieve efficient anaerobic cultivation without expensive equipment, meeting the needs of grassroots laboratories for high-frequency, low-cost microbial cultivation and promoting the research progress of anaerobic microorganisms. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a front view of an anaerobic bacteria culture device.

[0020] Figure 2 This is a side view of an anaerobic bacteria culture device;

[0021] Figure 3 This is a top view of an anaerobic bacteria culture device.

[0022] Figure 4 A front view of three anaerobic bacteria culture devices;

[0023] Figure 5 Side view of three anaerobic bacteria culture devices;

[0024] Figure 6 This is a top view of three anaerobic bacteria culture devices.

[0025] In the diagram: 1-Top cover; 2-Cylinder body; 3-Reaction tank; 4-Gas outlet pipe; 5-Liquid inlet pipe; 6-Liquid outlet pipe; 7-First valve; 8-Second valve; 9-Sealing cover; 10-Sealing ring; 11-Inclined bottom surface. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] The purpose of this invention is to provide an anaerobic bacteria culture device to solve the problems existing in the prior art. It has a simple structure, low cost, and is suitable for small-scale constant temperature culture equipment, thereby promoting the research progress of anaerobic microorganisms.

[0028] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the utility model will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0029] This invention provides an anaerobic bacteria culture device, such as... Figures 1-3As shown, the apparatus includes a culture chamber and a reaction tank 3. The culture chamber is used to house the anaerobic bacteria culture device, and the reaction tank 3 is used to generate CO2 gas. The culture chamber includes a cylinder 2 and a top cover 1. The top cover 1 and the cylinder 2 are detachably and sealed together. The cylinder 2 is connected to the gas outlet of the reaction tank 3. The top cover 1 has a gas outlet pipe 4, and a first valve 7 is installed on the gas outlet pipe 4. By adding specific chemical reagents to the reaction tank 3, a chemical reaction occurs, generating CO2 gas. The CO2 gas continuously enters the culture chamber. Utilizing the principle of gas replacement, the denser CO2 gas fills the entire culture chamber from bottom to top, expelling the relatively less dense air from the top gas outlet pipe 4, thereby forming a stable anaerobic environment. This makes the gas replacement process efficient and orderly. For basic laboratories, compared with the expensive equipment required for traditional anaerobic culture, the anaerobic bacteria culture device has a simple structure, low cost, and is reusable, which can meet their high-frequency, low-cost microbial culture needs, effectively reducing long-term operating costs and thus promoting the research progress of anaerobic microorganisms.

[0030] In a further preferred embodiment of this invention, the anaerobic bacteria cultivation device further includes an anaerobic indicator, which is placed in the cultivation chamber. Preferably, the anaerobic indicator is methylene blue, and the preparation method is as follows: 0.02g of methylene blue, 4g of ascorbic acid, 5g of soapstone, and 0.02g of CTMAB are added to 100ml of distilled water and stirred thoroughly. Then, NaOH solution is added dropwise to adjust the pH to 11. After the blue color fades in the solution, 0.015g of rose red is added. Paper threads are immersed in the solution for 24 hours, then drained and vacuum-dried at 50℃ for 15 minutes. The paper threads soaked in the methylene blue indicator are then vertically pasted onto the inner wall of the cultivation chamber. The degree of anaerobic activity in the equipment can be determined based on the color change of the paper threads.

[0031] In a further preferred embodiment of this invention, the reaction tank 3 is fixedly installed at the bottom of the cylinder 2. The side wall of the reaction tank 3 has an inlet and an outlet. The inlet is connected to and communicates with the inlet pipe 5, and the outlet is connected to and communicates with the outlet pipe 6. A second valve 8 is installed on the inlet pipe 5, and a sealing cap 9 is connected to the end of the outlet pipe 6. These gases will continuously enter the culture chamber. New reagents for generating carbon dioxide can be periodically added through the inlet pipe 5, while the outlet pipe 6 discharges the reacted reagents, ensuring a continuous generation of CO2 gas within the reaction tank 3 and maintaining a stable anaerobic environment within the culture chamber.

[0032] In a further preferred embodiment of this utility model, both the first valve 7 and the second valve 8 are ball valves. The valve status can be intuitively determined by observing the position of the ball valve handle. When the ball channel is parallel to the pipeline axis, it is fully open; when it is perpendicular, it is fully closed, thus avoiding misoperation.

[0033] In a further preferred embodiment of this invention, the bottom surface of the reaction tank 3 is an inclined bottom surface 11, and a liquid outlet pipe 6 is provided on the side wall connected to the lowest end of the inclined bottom surface 11. The bottom surface of the liquid outlet pipe 6 is flush with the bottom surface of the reaction tank 3. The inclined bottom surface 11 of the reaction tank 3 and the placement of the liquid outlet pipe 6 at the lowest end, flush with the bottom surface, allow the reacted liquid to naturally converge to the liquid outlet pipe 6 using gravity, ensuring complete and residue-free discharge of the liquid. This prevents waste liquid from accumulating in the reaction tank 3 and affecting the subsequent chemical reagent reaction effect. Furthermore, the natural flow of the drainage process eliminates the need for additional suction equipment, reducing operational complexity and equipment costs, making it particularly suitable for the needs of grassroots laboratories for convenient and low-cost equipment.

[0034] In a further preferred embodiment of this utility model, the anaerobic bacteria cultivation device also includes a sealing ring 10. An annular groove is formed on the outer wall of the top cover 1, and the sealing ring 10 is engaged within this groove. After the top cover 1 is inserted into the cylinder 2, the sealing ring 10 seals the annular gap between the outer wall of the top cover 1 and the inner wall of the cylinder 2. The engagement design between the annular groove on the outer wall of the top cover 1 and the sealing ring 10 tightly fills the annular space between the top cover 1 and the inner wall of the cylinder 2. This not only effectively isolates external oxygen intrusion and prevents leakage of internal anaerobic gas, ensuring a stable anaerobic environment within the cultivation chamber, but also facilitates convenient disassembly and maintenance, reducing processing precision requirements and operating costs.

[0035] In a further preferred embodiment of this invention, both the culture chamber and the reaction tank 3 are made of transparent material. The transparent culture chamber allows direct observation of the microbial growth status, eliminating the need for frequent opening and sampling, thus improving experimental efficiency and reducing the risk of contamination. More preferably, the surface of the transparent culture chamber is treated with a special coating (such as an acid-resistant coating) to prevent fogging or embrittlement after prolonged use.

[0036] In a further preferred embodiment of this invention, the culture chamber and reaction tank 3 are made of polytetrafluoroethylene (PTFE), as are the gas outlet pipe 4, liquid inlet pipe 5, and liquid outlet pipe 6. PTFE offers advantages such as long service life, low maintenance requirements, and high reliability. The liquid inlet pipe 5 has an inner diameter of 20 mm, a wall thickness of 2 mm, and the bottom of the liquid inlet pipe is 20 mm above the bottom of the reaction tank.

[0037] Furthermore, to accommodate petri dish racks and test tube racks of different sizes, multiple culture chambers of different sizes and matching reaction tanks can be designed according to the dimensions of different petri dish racks and test tube racks, meeting the requirements for the cultivation of anaerobic microorganisms in different ways and in different quantities, thus avoiding waste of resources. For example, Figures 4-6As shown, the system includes a large culture chamber, a large reaction vessel matching the size of the large culture chamber, two smaller culture chambers, and a small reaction vessel matching the size of the smaller culture chambers. The large culture chamber has an inner diameter of 220 mm, a wall thickness of 3 mm, and a height of 255 mm. The large reaction vessel has dimensions of 240 mm in length, 290 mm in width, and 43.96 mm in height. The small culture chambers have an inner diameter of 120 mm, a wall thickness of 3 mm, and a height of 255 mm. The small reaction vessels have dimensions of 140 mm in length, 140 mm in width, and 43.96 mm in height.

[0038] This utility model uses specific examples to illustrate its principles and implementation methods. The above description of the embodiments is only for the purpose of helping to understand the method and core idea of ​​this utility model. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the idea of ​​this utility model. In summary, the content of this specification should not be construed as a limitation of this utility model.

Claims

1. An anaerobic bacteria cultivation device, characterized in that: The device includes a culture chamber and a reaction tank. The culture chamber is used to house an anaerobic bacteria culture device, and the reaction tank is used to generate CO2 gas. The culture chamber includes a cylindrical body and a top cover. The cylindrical body is open at both ends. The top cover is detachably and sealed to one end of the cylindrical body. The other end of the cylindrical body is connected to the gas outlet of the reaction tank. The top cover has a gas outlet pipe, and a first valve is installed on the gas outlet pipe.

2. The anaerobic bacteria cultivation device according to claim 1, characterized in that: It also includes an anaerobic indicator, which is disposed in the culture chamber.

3. The anaerobic bacteria cultivation device according to claim 1, characterized in that: The reaction tank is fixedly installed at the bottom of the cylinder. The side wall of the reaction tank is provided with an inlet and an outlet. The inlet is connected to and communicates with the inlet pipe, and the outlet is connected to and communicates with the outlet pipe. A second valve is provided on the inlet pipe, and a sealing cap is connected to the end of the outlet pipe.

4. The anaerobic bacteria cultivation device according to claim 3, characterized in that: Both the first valve and the second valve are ball valves.

5. The anaerobic bacteria cultivation device according to claim 3, characterized in that: The air outlet pipe, the liquid inlet pipe, and the liquid outlet pipe are made of polytetrafluoroethylene.

6. The anaerobic bacteria cultivation device according to claim 3, characterized in that: The bottom surface of the reaction tank is sloping, and the liquid outlet pipe is provided on the side wall connected to the lowest end of the sloping bottom surface.

7. The anaerobic bacteria cultivation device according to claim 6, characterized in that: The bottom surface of the outlet pipe is flush with the bottom surface of the reaction tank.

8. The anaerobic bacteria cultivation device according to claim 1, characterized in that: It also includes a sealing ring. The outer side wall of the top cover has an annular groove. The sealing ring is engaged in the annular groove. The top cover is inserted into the cylinder. The sealing ring can seal the annular gap between the outer side wall of the top cover and the inner side wall of the cylinder.

9. The anaerobic bacteria cultivation device according to claim 1, characterized in that: Both the culture chamber and the reaction tank are made of transparent material.

10. The anaerobic bacteria cultivation device according to claim 9, characterized in that: The culture chamber and the reaction tank are made of polytetrafluoroethylene.

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