An apparatus for plant genetic transformation culture
By designing a cultivation device with different combinations of caps with varying scale values and heights, the problems of existing devices in accurately controlling the amount of culture medium and their poor versatility have been solved. This approach achieves precise control and wide applicability, reduces costs, and promotes healthy plant growth.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- CHINA TOBACCO YUNNAN IND
- Filing Date
- 2025-07-01
- Publication Date
- 2026-07-03
AI Technical Summary
Existing plant genetic transformation culture devices are difficult to control the amount of culture medium precisely, resulting in waste and increased costs. At the same time, they have poor versatility and cannot be applied to the culture needs of a variety of plants.
A device comprising a petri dish and a cap for combined use has been designed. The petri dish has scale values on its side and caps of different heights, along with vent holes, for precise control of the amount of culture medium at each stage, and is suitable for genetic transformation culture of different plants.
It enables precise control of the culture medium volume, reduces costs, expands the scope of application of the device, and facilitates observation and gas exchange, thus promoting healthy plant growth.
Smart Images
Figure CN224439954U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of plant tissue culture technology, and in particular to an apparatus for plant genetic transformation culture. Background Technology
[0002] In plant genetic transformation, subculture, differentiation culture, and rooting culture are crucial steps in obtaining rooted seedlings. The required amount of culture medium varies at different stages; however, existing culture devices struggle to precisely control the medium volume, leading to waste and increased costs. Furthermore, existing devices lack versatility and cannot be flexibly applied to the post-transformation culture of various plant species.
[0003] Therefore, there is an urgent need for a versatile culture device that can precisely control the amount of culture medium to meet the needs of plant genetic transformation culture. Utility Model Content
[0004] The technical problem to be solved by this utility model is to provide a device for plant genetic transformation culture that can be precisely controlled and has strong versatility, and can meet the needs of plant genetic transformation culture.
[0005] The technical problem to be solved by this utility model is achieved through the following technical solution:
[0006] An apparatus for plant genetic transformation culture includes a culture dish and a culture bottle cap used in combination. The side of the culture dish is provided with graduation values, including a minimum graduation for subculture, an intermediate graduation for differentiation culture, and a maximum graduation for rooting culture. The culture bottle cap includes a subculture bottle cap, a differentiation culture bottle cap, and a rooting culture bottle cap. The top of each of the subculture bottle cap, the differentiation culture bottle cap, and the rooting culture bottle cap is provided with a vent hole.
[0007] Preferably, in the above technical solution, the petri dish is further provided with at least one partition plate, which is used to divide the petri dish into multiple spaces.
[0008] Preferably, in the above technical solution, the number of petri dishes is one or three. When the number is one, the side of the petri dish is simultaneously provided with a minimum scale, a middle scale, and a maximum scale; when the number is three, the side of the three petri dishes is respectively provided with a minimum scale, a middle scale, and a maximum scale.
[0009] Preferably, in the above technical solution, when the number of the culture dishes is one, the lowest scale, the middle scale, and the highest scale are distributed at equal intervals along the circumference of the culture dish.
[0010] Preferably, in the above technical solution, the lowest scale, the middle scale, and the highest scale are set at equal or unequal intervals along the circumference of the culture dish.
[0011] Preferably, in the above technical solution, the height of the subculture bottle cap is less than the height of the differentiation culture bottle cap, and the height of the differentiation culture bottle cap is less than the height of the rooting culture bottle cap.
[0012] Preferably, in the above technical solution, the culture dish is a transparent culture dish, which is used in combination with any one of the subculture bottle cap, differentiation culture bottle cap, or rooting culture bottle cap.
[0013] Preferably, in the above technical solutions, when used in combination, the upper edge of the culture dish is in contact with the lower edge of the subculture bottle cap, differentiation culture bottle cap, or rooting culture bottle cap.
[0014] Preferably, in the above technical solution, the vent is located at the center of the top of the culture bottle cap to ensure gas exchange during the culture process and provide a suitable gaseous environment for plant growth.
[0015] The above-mentioned technical solution of this utility model has the following beneficial effects:
[0016] (1) Precise control of culture medium quantity: By using the scale on the petri dish and the caps of different heights, the amount of culture medium required for each culture stage can be accurately determined, avoiding waste of culture medium and effectively reducing culture costs.
[0017] (2) Improve the versatility of the device: This device is not only suitable for the genetic transformation and culture of tobacco, but with proper adjustment and application, it can also be used for the genetic transformation and culture of other plants such as wheat and corn, thus expanding the scope of application of the device.
[0018] (3) Facilitates observation and gas exchange: Transparent petri dishes allow researchers to observe the growth status of plants at any time, and the ventilation holes at the top of the cap ensure gas exchange in the culture environment, which is conducive to the healthy growth of plants. Attached Figure Description
[0019] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments of the present invention and, together with their description, serve to explain the principles of the present invention.
[0020] Figure 1 A top view of the petri dish (including the separators);
[0021] Figure 2 Schematic diagram of culture bottle caps of different heights;
[0022] Figure 3 This is a side view of the apparatus used for subculture.
[0023] Figure 4 This is a side view of the apparatus during differentiation culture;
[0024] Figure 5 This is a side view of the apparatus used for rooting culture.
[0025] In the diagram: 1- Petri dish, 11- Subculture graduations, 12- Differentiation culture graduations, 13- Rooting culture graduations, 14- Separator, 2- Culture bottle cap, 21- Subculture bottle cap, 22- Differentiation culture bottle cap, 23- Rooting culture bottle cap, 24- Vent hole. Detailed Implementation
[0026] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that, unless otherwise specifically stated, the relative arrangement, numerical expressions, and values of the components and steps set forth in these embodiments do not limit the scope of the present invention.
[0027] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, and the materials and reagents used are commercially available. Unless otherwise specified, the equipment used in the experiments is well known to those skilled in the art.
[0028] An apparatus for plant genetic transformation culture includes a culture dish 1 and a culture bottle cap 2 used in combination. The side of the culture dish 1 is provided with scale values, including a minimum scale 11 for subculture, an intermediate scale 12 for differentiation culture, and a maximum scale 13 for rooting culture. The culture bottle cap 2 includes a subculture bottle cap 21, a differentiation culture bottle cap 22, and a rooting culture bottle cap 23. The top of each of the subculture bottle cap 21, the differentiation culture bottle cap 22, and the rooting culture bottle cap 23 is provided with a vent hole 24.
[0029] Furthermore, the number of petri dishes 1 can be one or three. When there is only one petri dish 1, the side of the petri dish 1 is simultaneously provided with a minimum scale 11, a middle scale 12, and a maximum scale 13. When there are three petri dishes 1, the sides of the three petri dishes 1 are respectively provided with a minimum scale 11, a middle scale 12, and a maximum scale 13. When there is only one petri dish 1, the minimum scale 11, the middle scale 12, and the maximum scale 13 are distributed at equal intervals along the circumference of the petri dish 1. Of course, this application is not limited to this, and two or other numbers of petri dishes can also be provided as needed.
[0030] Furthermore, at least one partition plate 14 is provided inside the petri dish 1 to divide the petri dish 1 into multiple spaces. For example... Figure 1The example shown is divided into three spaces. Of course, this application is not limited to this, and those skilled in the art can divide it into two, three, four or more spaces as needed.
[0031] Furthermore, the culture dish 1 can be used in combination with any one of the following: subculture bottle cap 21, differentiation culture bottle cap 22, or rooting culture bottle cap 23. When used in combination, the upper edge of the culture dish 1 is in contact with the lower edge of the subculture bottle cap 21, differentiation culture bottle cap 22, or rooting culture bottle cap 23.
[0032] Furthermore, the height of the subculture bottle cap 21 is less than the height of the differentiation culture bottle cap 22, and the height of the differentiation culture bottle cap 22 is the same as the height of the rooting culture bottle cap 23. In the embodiments of this application, the heights of the three are preferably in a multiple relationship, but this application is not limited thereto.
[0033] Furthermore, the lowest scale 11, the middle scale 12, and the highest scale 13 are evenly distributed along the circumference of the petri dish 1.
[0034] Furthermore, the vent 24 is located in the center of the top of the culture bottle cap 2 to ensure gas exchange during the culture process and provide a suitable gas environment for plant growth.
[0035] The device of this application, when in use:
[0036] (1) Preparation: Select appropriate petri dishes and caps according to the genetic transformation culture requirements of different plants. Perform strict cleaning and sterilization on the petri dishes and caps to ensure a sterile environment.
[0037] (2) Culture medium addition:
[0038] When subculturing, use the subculture bottle cap 21 in conjunction with the culture dish with the minimum mark 11, and add an appropriate amount of culture medium according to the minimum mark.
[0039] During differentiation culture, use a differentiation culture bottle cap 22 and a culture dish with a middle mark 12, and add culture medium according to the middle mark 12;
[0040] During rooting culture, a rooting culture bottle cap 23 and a culture dish with the highest graduation mark 13 are used, and culture medium is added according to the amount specified on the rooting culture bottle cap 23. This combination method allows for precise control of the amount of culture medium used at different culture stages.
[0041] (3) Plant inoculation and culture: On a sterile operating table, plant materials that have undergone genetic transformation are inoculated into culture dishes with added culture medium, the corresponding culture bottle caps are covered, and the plants are placed in a suitable culture environment (such as a light incubator). Temperature, light, humidity and other conditions are set according to the requirements of different plants and culture stages for culture.
[0042] (4) Cultivation process management: During the cultivation process, observe the plant growth regularly through transparent petri dishes and adjust the cultivation environment parameters in a timely manner according to the plant's growth needs. At the same time, pay attention to maintaining the airtightness and permeability of the cultivation device to ensure normal plant growth.
[0043] Meanwhile, the design parameters of the device in this application have been optimized, and it can also be applied to the culture of plants such as wheat and corn after genetic transformation.
[0044] Although the present invention has been disclosed above with reference to embodiments, it is not intended to limit the present invention. Any person skilled in the art can make various different choices and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention is defined by the claims and their equivalents.
Claims
1. An apparatus for plant genetic transformation culture, characterized by, The system includes a culture dish (1) and a culture bottle cap (2) used in combination. The culture dish (1) has scale values on its side, including the lowest scale (11) for subculture, the middle scale (12) for differentiation culture, and the highest scale (13) for rooting culture. The culture bottle cap (2) includes a subculture bottle cap (21), a differentiation culture bottle cap (22), and a rooting culture bottle cap (23). The top of the subculture bottle cap (21), the differentiation culture bottle cap (22), and the rooting culture bottle cap (23) are all provided with ventilation holes (24).
2. The device for plant genetic transformation culture according to claim 1, characterized in that, The culture dish (1) is also provided with at least one partition plate (14) for dividing the culture dish (1) into multiple spaces.
3. The device for plant genetic transformation culture according to claim 1, wherein, The number of the culture dishes (1) is one or three. When there is one culture dish (1), the side of the culture dish (1) is simultaneously provided with the lowest scale (11), the middle scale (12) and the highest scale (13); when there are three culture dishes (1), the side of the three culture dishes (1) is provided with the lowest scale (11), the middle scale (12) and the highest scale (13) respectively.
4. The device for plant genetic transformation culture according to claim 3, characterized in that, When there is only one culture dish (1), the lowest scale (11), the middle scale (12) and the highest scale (13) are distributed at equal intervals along the circumference of the culture dish (1).
5. The device for plant genetic transformation culture according to claim 1, wherein, The height of the subculture bottle cap (21) is less than the height of the differentiation culture bottle cap (22), and the height of the differentiation culture bottle cap (22) is less than the height of the rooting culture bottle cap (23).
6. The device for plant genetic transformation culture according to claim 1, wherein, The culture dish (1) is a transparent culture dish, and the culture dish (1) is used in combination with any one of the subculture bottle cap (21), differentiation culture bottle cap (22), or rooting culture bottle cap (23).
7. The apparatus for plant genetic transformation culture according to claim 6, characterized in that, When used in combination, the upper edge of the culture dish (1) is in contact with the lower edge of the subculture bottle cap (21), the differentiation culture bottle cap (22), or the rooting culture bottle cap (23).
8. The device for plant genetic transformation culture according to claim 1, wherein, The vent (24) is located at the center of the top of the culture bottle cap (2) to ensure gas exchange during the culture process and provide a suitable gas environment for plant growth.