Caragana korshinskii 13c stable isotope tracer marking bag

Abstract

The utility model relates to plant isotopic tracer technology field especially discloses a kind of caragana 13C stable isotopic tracer marking bag, including bag body and bag mouth, marking bag is fixed outside caragana, air pipe bag is docked with the device filled with 13C stable isotopic gas, gas injection makes plant contact marking gas, after inflation, without disassembling inflation device, connect suction tube bag and suction device, extract mixed gas in bag, it is convenient for subsequent component analysis, finally air pipe bag and suction tube bag are knotted respectively, realize marking bag closure, avoid the risk of bag body breakage caused by repeatedly disassembling equipment, adopt integrated tube bag, prevent hole tearing expansion problem, simultaneously without the aid of additional sealing material, both retain marking bag internal complete space, avoid extruding caragana growth area, whereby the data obtained can truly and accurately reflect caragana actual carbon metabolism mechanism, guarantee the reliability of experimental data.

Description

Technical Field

[0001] This utility model relates to the field of plant isotope tracing technology, and in particular to a stable isotope 13C tracer bag for Caragana korshinskii. Background Technology

[0002] Caragana korshinskii 13C Stable Isotope Marking Bags: As an important ecological restoration and forage shrub in arid and semi-arid regions, the study of its carbon metabolism mechanism is of great significance for revealing the carbon cycle law of desert ecosystems. 13C stable isotope marking technology can accurately analyze the carbon assimilation, allocation and transformation process of Caragana korshinskii by marking the carbon source fixed by plant photosynthesis, providing key data support for the construction of ecosystem carbon balance models.

[0003] In existing technologies, plant 13C stable isotope labeling experiments are mainly divided into greenhouses and fields. Considering the convenience of field experiments, labeling bags are often used to label Caragana korshinskii. The common material for labeling bags is transparent polyvinyl chloride. In order to connect with the air intake and inflation device, the labeling bag needs to be perforated.

[0004] However, polyvinyl chloride (PVC) material itself is highly flexible and lacks structural support. During repeated connection of inflation and deflation equipment, the opening is prone to tearing and expansion due to uneven stress, causing the hole size to continuously increase. When the marking bag is sealed after the experiment, due to the damage and deformation at the opening, it is necessary to use additional sealing material to forcibly seal the hole. This not only reduces the effective space inside the marking bag and squeezes the growth area of ​​Caragana korshinskii, affecting the normal physiological activities of the plant, but also reduces the reliability of the experimental data. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a 13C stable isotope tracer bag for Caragana korshinskii. This solves the problem that polyvinyl chloride (PVC) material, while flexible, lacks structural support. During repeated connection to inflation and deflation equipment, uneven stress at the openings easily causes tearing and expansion, leading to continuously increasing hole size. When the bag is sealed after the experiment, the damage and deformation at the openings necessitate the use of additional sealing materials to forcibly seal the holes. This not only reduces the effective internal space of the bag and compresses the Caragana korshinskii's growth area, affecting the plant's normal physiological activities, but also reduces the reliability of experimental data.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A 13C stable isotope tracer bag for Caragana korshinskii includes a bag body and a bag opening. The bag opening is located at the top of the bag body. An inflation tube and an intake tube are provided on the side of the bag body away from the bag opening for connecting to external equipment. Two openings are provided on the outside of the bag body, which communicate with the inside of the bag body. One end of the inflation tube and the intake tube are respectively fixedly connected to the inside of the two openings. An identification bag for holding plant information labels is provided on the outside of the bag body.

[0008] Preferably, the label bag has an opening on the outside, and a label is placed inside the opening.

[0009] Preferably, the two ends of the bag body near the bag opening are fixedly connected with a first binding rope and a second binding rope, respectively.

[0010] Preferably, both the inflation tube bag and the suction tube bag have silicone tubes fixedly connected to their ends away from the opening. The silicone tubes have good flexibility and sealing properties, which can ensure smooth gas transmission and facilitate connection and disconnection with the inflation device and suction device, thus improving the convenience of experimental operation.

[0011] Preferably, an adhesive strip is fixedly installed inside the opening, and release paper is laid on the outside of the adhesive strip.

[0012] Preferably, the label bag is fixedly connected to the outside with an adhesive strip, and the outside of the adhesive strip is covered with release paper.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The labeling bag was fixed to the outside of the *Caragana korshinskii* plant. The inflatable tube bag was connected to the device for filling 13C stable isotope gas. Gas was injected to allow the plant to come into contact with the labeling gas. After inflation, without disassembling the inflation device, the suction tube bag was connected to the suction device, and the mixed gas inside the bag was extracted for subsequent component analysis. Finally, the inflatable tube bag and the suction tube bag were tied separately to seal the labeling bag, avoiding the risk of bag damage caused by repeated disassembly of the equipment. The integrated tube bag eliminated the problem of hole tearing and expansion, and no additional sealing material was needed. This preserved the complete internal space of the labeling bag and avoided compressing the growth area of ​​the *Caragana korshinskii* plant. The data obtained in this way can accurately reflect the actual carbon metabolism mechanism of *Caragana korshinskii* plant and ensure the reliability of the experimental data. Attached Figure Description

[0015] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings.

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0017] Figure 2 This utility model Figure 1 Top view of the central bag structure;

[0018] Figure 3 This utility model Figure 1 Structural diagram of the label bag;

[0019] Figure 4 This utility model Figure 1 Enlarged structural diagram at point A in the middle.

[0020] Illustrations: 1. Bag body; 2. Bag opening; 3. Inflation tube bag; 4. Suction tube bag; 5. Opening; 6. Label bag; 7. Opening; 8. Label paper; 9. Tie rope one; 10. Tie rope two; 11. Silicone tube; 12. Adhesive strip one; 13. Release paper one; 14. Adhesive strip two; 15. Release paper two. Detailed Implementation

[0021] This application provides a 13C stable isotope tracer bag for Caragana korshinskii, effectively solving the technical problem that polyvinyl chloride (PVC) material itself is highly flexible but lacks structural support. During repeated connection of inflation and deflation equipment, uneven stress at the openings easily causes tearing and expansion, resulting in continuously increasing hole size. When the bag is sealed after the experiment, the damage and deformation at the openings necessitate the use of additional sealing materials to forcibly seal the holes. This not only reduces the effective space inside the bag and compresses the growth area of ​​Caragana korshinskii, affecting the normal physiological activities of the plant, but also reduces the reliability of experimental data.

[0022] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the technical solution in this application effectively solves the problem that polyvinyl chloride (PVC) material itself is highly flexible but lacks structural support. During repeated connection of inflation and deflation devices, the opening is prone to tearing and expansion due to uneven stress, resulting in a continuous increase in the size of the hole. When the marking bag is sealed after the experiment, due to the damage and deformation at the opening, additional sealing material must be used to forcibly seal the hole. This not only reduces the effective space inside the marking bag and squeezes the growth area of ​​Caragana korshinskii, affecting the normal physiological activities of the plant, but also reduces the reliability of the experimental data. The overall approach is as follows:

[0023] To address the problems existing in the prior art, this utility model provides a 13C stable isotope tracer bag for Caragana korshinskii, including a bag body 1 and a bag opening 2. The bag opening 2 is located at the upper end of the bag body 1. An inflation tube bag 3 and an air suction tube bag 4 for connecting to external equipment are provided on the side of the bag body 1 away from the bag opening 2. The inflation tube bag 3, the air suction tube bag 4 and the marking bag are made of the same material. Two openings 5 ​​are provided on the outside of the bag body 1, and the openings 5 ​​communicate with the inside of the bag body 1. One end of the inflation tube bag 3 and the air suction tube bag 4 are respectively fixedly connected to the inside of the two openings 5.

[0024] The outer side of the bag body 1 is provided with a label bag 6 for holding plant information labels. The label bag 6 has an opening 7 on the outside, and a label paper 8 is placed inside the opening 7.

[0025] The two ends of the bag body 1 near the bag opening 2 are respectively fixedly connected with binding rope 9 and binding rope 10.

[0026] Both the inflation tube bag 3 and the suction tube bag 4 have silicone tubes 11 fixedly connected to the ends away from the opening 5.

[0027] An adhesive strip 12 is fixedly installed inside the opening 7, and release paper 13 is laid on the outside of the adhesive strip 12.

[0028] The label bag 6 is fixedly connected to the outside by an adhesive strip 2 14, and the outside of the adhesive strip 2 14 is covered with release paper 2 15.

[0029] Example 1

[0030] At the start of the experiment, the marking bag was placed over the Caragana korshinskii through bag opening 2. Bag opening 2 was then sealed using binding ropes 1-9 and 2-10. The silicone tube 11 on the inflation tube bag 3 was connected to the inflation device already filled with 13C stable isotope gas. The inflation device valve was opened, and the 13C stable isotope gas was slowly injected into the marking bag according to the preset gas flow rate and time, ensuring full contact between the Caragana korshinskii and the marking gas. After inflation, without disassembling the inflation device, the silicone tube 11 on the suction tube bag 4 was directly connected to the suction device. The suction device was then activated to extract excess gas from the marking bag and the mixed gas generated by the Caragana korshinskii during absorption, allowing for subsequent analysis and detection of the gas composition. The design of the inflation tube bag 3 and suction tube bag 4 eliminates the need to disassemble the inflation device and reconnect it to the suction device, reducing experimental time and avoiding delays caused by… Repeated disassembly and reassembly can lead to gas leaks and sample contamination. Finally, by tying the inflation tube bag 3 and the suction tube bag 4 tightly, the marking bag is completely sealed. Compared to traditional methods, this solution eliminates the need for direct openings in the bag body 1. The integrated tube bag replaces the original connection method, avoiding the risk of damage to the bag body 1 caused by repeated disassembly and preventing the tearing and expansion of holes. Furthermore, this sealing process does not require additional sealing materials, preserving the complete and effective space inside the marking bag, preventing compression of the Caragana korshinskii growth area, and ensuring that the plant's photosynthetic and respiratory physiological activities are not disturbed. This makes the carbon metabolism process closer to the natural state, and the resulting carbon assimilation and allocation data can accurately reflect the actual carbon metabolism mechanism of Caragana korshinskii, providing precise data support for research on carbon cycling in desert ecosystems and ensuring the reliability of experimental data.

[0031] Example 2

[0032] After obtaining relevant information about the *Caragana korshinskii* plant, such as plant number, planting location, and growth stage, it is written on label paper 8. Label paper 8 is then placed inside the opening 7 of the label bag 6. First, the release paper 13 outside the adhesive strip 12 on the inside of the label bag 6 is peeled off. The label bag 6 is then pressed to seal the inside through the adhesive strip 12, securing label paper 8 inside the label bag 6 to prevent it from falling off or shifting during the experiment. Next, the release paper 15 outside the adhesive strip 24 at the end of the label bag 6 furthest from the opening 7 is peeled off. Depending on the experimental requirements, the label bag 6 is then secured to a conspicuous location outside the bag body 1 using the adhesive strip 24, in a position that does not obstruct gas exchange. This allows for long-term use in field experimental environments without damage, ensuring the integrity of information recording and facilitating quick and accurate identification and recording of plant information by experimental personnel, thus enabling further operations and ensuring the reliability of experimental results.

[0033] Finally, it should be noted that the above embodiments are merely examples for clearly illustrating the present invention and are not intended to limit the implementation. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations. However, obvious variations or modifications derived therefrom are still within the protection scope of this invention.

Claims

1. A 13C stable isotope tracer bag for Caragana korshinskii, comprising a bag body (1) and a bag opening (2), wherein the bag opening (2) is located at the upper end of the bag body (1), characterized in that, The side of the bag body (1) away from the bag opening (2) is provided with an inflation tube bag (3) and an air suction tube bag (4) for connecting external equipment. The bag body (1) has two openings (5) on its outside. The openings (5) are connected to the inside of the bag body (1). One end of the inflation tube bag (3) and the air suction tube bag (4) are respectively fixedly connected to the inside of the two openings (5). The bag body (1) has an external label bag (6) for holding plant information labels.

2. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 1, characterized in that, The label bag (6) has an opening (7) on the outside, and a label paper (8) is placed inside the opening (7).

3. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 1, characterized in that, The two ends of the bag body (1) near the bag opening (2) are respectively fixedly connected with a first rope (9) and a second rope (10).

4. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 1, characterized in that, Both the inflatable tube bag (3) and the suction tube bag (4) are fixedly connected to a silicone tube (11) at the end away from the opening (5).

5. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 2, characterized in that, An adhesive strip (12) is fixedly installed inside the opening (7).

6. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 5, characterized in that, Release paper (13) is laid on the outside of the adhesive strip (12).

7. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 1, characterized in that, The label bag (6) is externally fixed with adhesive strip two (14).

8. The 13C stable isotope tracer bag for *Caragana korshinskii* as described in claim 7, characterized in that, Release paper 2 (15) is laid on the outside of the adhesive strip 2 (14).

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