A tumor primary cell culture device
Patent Information
- Application Number
- CN202522643825.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-12
- Publication Date
- 2026-11-03
- Estimated Expiration
- 2035-12-12
AI Technical Summary
When multiple culture dishes are placed in the depth direction in a cell culture incubator, sampling becomes inconvenient, requiring researchers to reach deep into the cell to operate, which brings inconvenience and the potential risk of the dishes falling.
A tumor primary cell culture device was designed, which adopts a telescopic positioning rod and an arrangement plate structure. The interlocking of protrusions and concave blocks enables the limiting and movement of the culture dish, facilitating sample collection by experimenters. The sealing plate improves stability and prevents the culture dish from being excessively pulled out.
This method enables convenient sampling of petri dishes, reduces the difficulty of experimental operations, improves the safety and stability of the sampling process, and prevents water accumulation in petri dishes in high humidity environments.
Smart Images
Figure CN224930578U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of cell culture device technology, and more specifically, to a tumor primary cell culture device. Background Technology
[0002] Primary tumor cell culture refers to the first in vitro culture of cells directly isolated from a patient's tumor tissue. Because it preserves the genetic characteristics, heterogeneity, and some in vivo microenvironmental features of the source tumor to the greatest extent, cultured cells from the first to the tenth generation are collectively referred to as primary cell culture. The genetic characteristics of cells obtained by this culture method are close to those in vivo, making it suitable for studying cell morphology, function, and differentiation. During the culture process, the culture environment needs to be stabilized in a carbon dioxide incubator.
[0003] During the incubation process, the support frame is placed on the support plate inside the incubator. Usually, the support plate has a pull-out structure. When the culture dish is removed, the support plate is pulled out. However, cell culture incubators have a certain depth. When multiple culture dishes are placed on a tray, they will gradually be placed along the depth of the tray, which requires the experimenter to reach deep into the incubator to retrieve the culture dishes, causing inconvenience to the sampling work.
[0004] Therefore, a primary tumor cell culture device is needed to solve the above problems. Utility Model Content
[0005] The summary section of this application is intended to provide a brief overview of the concepts, which will be described in detail in the detailed description section below. This summary section is not intended to identify key or essential features of the claimed technical solutions, nor is it intended to limit the scope of the claimed technical solutions.
[0006] To address the technical problems mentioned in the background section, some embodiments of this application provide a primary tumor cell culture device, comprising: a culture chamber body, the front end of which is hinged to a double-layered sealed door; the bottom of the culture chamber body has exhaust vents on both sides and a humidity vent in the middle, the exhaust vents and the humidity vent being connected to a temperature control system and a relative humidity control system, respectively; a front frame and a rear frame are fixedly installed on both inner side walls of the culture chamber body, the front frame and the rear frame having evenly distributed protrusions; a support frame is attached to four protrusions on the same horizontal plane; an evenly distributed arrangement plate is distributed on the support frame; a sealing plate is connected to the upper end of the support frame, and the sealing plate abuts against the arrangement plate.
[0007] Furthermore, the protrusion on the front frame is provided with a connecting hole, and a telescopic positioning rod is connected and installed in the connecting hole. The telescopic positioning rod is inserted and engaged with the arrangement plate. Each arrangement plate has a protrusion near the end of the telescopic positioning rod. The protrusion is shaped like a truncated cone and opens towards the telescopic positioning rod. The receiving frame, the sealing plate and the protrusion are inserted and engaged vertically. Two adjacent arrangement plates are connected end to end. The side end of the receiving frame is provided with a plug-in hole that communicates with the connecting hole.
[0008] Furthermore, the protruding entity on the rear frame is not provided with holes. The arrangement plate includes a concave block at the rear end and a protrusion at the front end. The concave block and the protrusion are respectively provided with a rod and a slot. The rod and the slot have the same radius and are inserted into each other. The concave block and the protrusion are arranged to complement each other. The arrangement plate is provided with a receiving groove.
[0009] Furthermore, the receiving groove is arranged in a ring shape to hold the culture dish, and the protrusion extends upward after bending to connect with the receiving frame and the sealing plate.
[0010] The beneficial effects of this application are as follows:
[0011] 1. Install the telescopic positioning rod on the connecting hole of the protrusion. The telescopic positioning rod is installed at the front of the frame, so that the rod at the telescopic positioning rod can be inserted into the protrusion of the front plate, thereby limiting the position of the plate. During culture observation, by pulling the plate forward, the telescopic positioning rod is pushed against the wall of the conical hole of the protrusion to overcome the spring force and retract, thereby pulling the plate forward. This moves the plate connected end to end forward, and moves the culture dishes placed on the plate forward, which facilitates the subsequent sampling of the culture dishes by the experimenter.
[0012] 2. By opening a connection hole at the protrusion of the front frame and installing a telescopic positioning rod in the connection hole, the telescopic positioning rod can automatically limit the front arrangement plate, thereby reminding the experimenter and preventing the arrangement plate from being pulled out too much, thus preventing the risk of the culture dish falling due to being pulled out of the incubator too much.
[0013] 3. A cavity is provided at the arrangement plate to reduce weight and the space created by the cavity makes it easy for the experimenter to hold. The sealing plate at the top abuts and limits the splicing of the two arrangement plates to prevent them from separating, thereby improving stability and preventing water accumulation problems caused by the high humidity environment inside the chamber during the cultivation process. Attached Figure Description
[0014] The accompanying drawings, which form part of this application, are used to provide a further understanding of the application and to make other features, objects, and advantages of the application more apparent. The illustrative embodiments and descriptions of this application are used to explain the application and do not constitute an undue limitation of the application.
[0015] Furthermore, throughout the accompanying drawings, the same or similar reference numerals denote the same or similar elements. It should be understood that the drawings are schematic, and the elements are not necessarily drawn to scale.
[0016] In the attached diagram:
[0017] Figure 1 This is a schematic diagram of the overall structure according to one embodiment of this application;
[0018] Figure 2 yes Figure 1 Internal structure diagram of the embodiment;
[0019] Figure 3 yes Figure 1 Internal structure diagram of the embodiment;
[0020] Figure 4 yes Figure 2 A partial cross-sectional view of point AA in the embodiment;
[0021] Figure 5 yes Figure 3 A partial cross-sectional view of BB in the embodiment;
[0022] Figure 6 yes Figure 3 A magnified view of a portion of point C in the embodiment.
[0023] Figure label:
[0024] 10. Incubator body; 11. Sealed door; 12. Exhaust vent; 13. Humidity vent; 14. Front frame; 15. Rear frame; 16. Protrusion; 17. Receiving frame; 18. Arrangement plate; 19. Sealing plate; 61. Connecting hole; 70. Insertion hole; 71. Telescopic positioning rod; 80. Concave block; 81. Protrusion block; 82. Insertion rod; 83. Slot; 84. Receiving groove. Detailed Implementation
[0025] Embodiments of this disclosure will now be described in more detail with reference to the accompanying drawings. While some embodiments of this disclosure are shown in the drawings, it should be understood that this disclosure can be implemented in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided to provide a more thorough and complete understanding of this disclosure. It should be understood that the accompanying drawings and embodiments of this disclosure are for illustrative purposes only and are not intended to limit the scope of protection of this disclosure.
[0026] It should also be noted that, for ease of description, only the parts relevant to the invention are shown in the accompanying drawings. Unless otherwise specified, the embodiments and features described in this disclosure can be combined with each other.
[0027] It should be noted that the concepts of "first" and "second" mentioned in this disclosure are used only to distinguish different devices, modules or units, and are not used to limit the order of functions performed by these devices, modules or units or their interdependencies.
[0028] It should be noted that, in the description of this disclosure, unless otherwise expressly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0029] It should be noted that, in this disclosure, unless otherwise expressly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.
[0030] It should be noted that the terms "a" and "a plurality of" used in this disclosure are illustrative rather than restrictive, and those skilled in the art should understand that, unless otherwise expressly indicated in the context, they should be understood as "one or more".
[0031] This disclosure will now be described in detail with reference to the accompanying drawings and embodiments.
[0032] Reference Figure 1-3A primary tumor cell culture device includes: a culture chamber body 10, with a double-layered sealed door 11 hinged to the front end of the culture chamber body 10. The sealed door 11 has a protective shell on the outside and a viewing window on the inside. Both the protective shell and the viewing window can rotate with the culture chamber body 10 and are sealed by a sealing gasket. The protective shell and the viewing window adopt existing structures, which will not be described in detail here. The bottom of the culture chamber body 10 has exhaust ports 12 on the left and right sides and a humidity vent 13 in the middle. The exhaust ports 12 and the humidity vent 13 are respectively connected to a temperature control system and a relative humidity control system to control the temperature (37°C) and humidity (>95%) inside the culture chamber body 10 to be maintained at an appropriate position. Front frames 14 and rear frames 15 are fixedly installed on the inner side walls of the culture chamber body 10. Both the front frames 14 and the rear frames 15 have protrusions 16 evenly distributed vertically. The frame 14 and the protrusion 16, and the rear frame 15 and the protrusion 16 are integrally formed. The four protrusions 16 on the same horizontal plane are connected to the support frame 17. The support frame 17 is distributed with evenly distributed front and rear plates 18. The upper end of the support frame 17 is connected to two left and right distributed sealing plates 19. The sealing plates 19 and the plates 18 abut against each other to allow the vertical movement of the straight plates 18. The front frame 14 and the rear frame 15 are made of stainless steel. The internal structure of the incubator body 10, such as the inner liner, is made of cold-rolled steel plate with powder coating. The inner liner mirror is made of stainless steel. The overall design of the incubator body 10 is a double-wall or rectangular layout to enhance heat preservation and space utilization. Other structures inside the incubator body 10, such as the air circulation system, door and sealing design and additional functional modules (such as HEPA filter, UV disinfection lamp, water level alarm and safety device), are connected and assembled with the existing structure. They are not shown in the figure and will not be described in detail here.
[0033] The receiving frame 17 and the arrangement plate 18 are made of stainless steel. The receiving frame 17 has evenly distributed holes, and the arrangement plate 18 has cavities. The receiving frame 17 prevents water accumulation through the holes, and the arrangement plate 18 reduces its weight through the cavities. The sealing plate 19 is connected to the left and right ends of the receiving frame 17 to restrict the upper displacement of the arrangement plate 18, so that the arrangement plate 18 can be moved back and forth under the guidance of the receiving frame 17 and the sealing plate 19 to be removed or sent into the receiving frame 17 for storage. A culture dish is placed on top of the arrangement plate 18 for cell culture.
[0034] Reference Figure 4A connecting hole 61 is provided at the protrusion 16 on the front frame 14. The connecting hole 61 extends horizontally and is perpendicular to the vertical plane of the protrusion 16. A telescopic positioning rod 71 is connected and installed in the connecting hole 61. The telescopic positioning rod 71 is telescopic and is inserted into the arrangement plate 18. Each arrangement plate 18 has a protrusion 81 near the end of the telescopic positioning rod 71. The protrusion 81 is a truncated cone facing the telescopic positioning rod 71. The receiving frame 17 and the sealing plate 19 are inserted vertically into the protrusion 16. Two adjacent arrangement plates 18 are connected end to end. The side end of the receiving frame 17 has a plug hole 70 communicating with the connecting hole 61. The receiving frame 17 and the sealing plate 19 are fixed together by rivets. The telescopic positioning rod 71 is installed on the connecting hole 61 opened in the protrusion 16. The telescopic positioning rod 71 is installed at the front frame 14, so that the rod at the telescopic positioning rod 71 can be inserted into the protrusion 81 of the front arrangement plate 18, thereby limiting the arrangement plate 18. When conducting culture observation, by pulling the arrangement plate 18 forward, the telescopic positioning rod 71 is pushed to retract against the spring force under the abutment of the conical hole wall of the protrusion 81, thereby pulling the arrangement plate 18 forward. This causes the end-to-end arrangement plate 18 to move forward, driving the culture dish placed on the arrangement plate 18 to move forward, which facilitates the subsequent removal of the culture dish by the experimenter.
[0035] Reference Figure 5 The protrusion 16 on the rear frame 15 is solid without holes. The arrangement plate 18 includes a concave block 80 at the rear end and a protrusion 81 at the front end. The concave block 80 and the protrusion 81 are respectively provided with a rod 82 and a slot 83. The rod 82 and the slot 83 have the same radius and are inserted and connected vertically. The concave block 80 and the protrusion 81 are arranged to complement each other vertically. The arrangement plate 18 has a receiving groove 84. The receiving groove 84 is used to place and support the culture dish to maintain stability. Through the arrangement plates 18 connected front and rear, the concave blocks 80 and the protrusion 81 at both ends of the arrangement plate 18 abut vertically and vertically, and the rod 82 and the slot 83 are inserted vertically to realize the synchronous movement of the arrangement plate 18 in the front and rear direction. After the abutting end of the concave block 80 and the protrusion 81 moves out of the lower end of the sealing plate 19, the two adjacent arrangement plates 18 can be separated.
[0036] Reference Figure 6 The receiving groove 84 is arranged in a ring shape to hold the culture dish, and the protrusion 16 is bent and extends upward to fit into the receiving frame 17 and the sealing plate 19.
[0037] The above description is merely a selection of preferred embodiments of this disclosure and an explanation of the technical principles employed. Those skilled in the art should understand that the scope of the invention involved in the embodiments of this disclosure is not limited to technical solutions formed by specific combinations of the above-described technical features, but should also cover other technical solutions formed by arbitrary combinations of the above-described technical features or their equivalents without departing from the above-described inventive concept. For example, technical solutions formed by substituting the above-described features with (but not limited to) technical features with similar functions disclosed in the embodiments of this disclosure.
Claims
1. A primary tumor cell culture device, comprising a culture chamber body (10), a sealed chamber door (11), an exhaust port (12), and a moisture vent (13), characterized in that: The incubator body (10) has a front frame (14) and a rear frame (15) fixedly installed on the inner side walls. The front frame (14) and the rear frame (15) are provided with protrusions (16) evenly distributed vertically. A support frame (17) is connected to the four protrusions (16) on the same horizontal plane. The support frame (17) has a plate (18) evenly distributed vertically. Two adjacent plates (18) are spliced together. A connecting hole (61) is provided on the protrusion (16) of the front frame (14). A telescopic positioning rod (71) is installed on the connecting hole (61) and is inserted into the plate (18). The side end of the support frame (17) is provided with a plug-in hole (70) communicating with the connecting hole (61).
2. The tumor primary cell culture device according to claim 1, characterized in that: The upper end of the receiving frame (17) is connected to two left and right distributed sealing plates (19). The sealing plates (19) abut against the arrangement plate (18) from top to bottom. The receiving frame (17) and the sealing plates (19) are riveted and fixed. The receiving frame (17), the sealing plates (19) and the protrusion (16) are inserted and matched from top to bottom.
3. The tumor primary cell culture device according to claim 1, characterized in that: The arrangement plate (18) includes a concave block (80) at the rear end and a protrusion (81) at the front end. The concave block (80) and the protrusion (81) are respectively provided with a rod (82) and a slot (83). The rod (82) and the slot (83) have the same radius and are inserted and connected vertically. The concave block (80) and the protrusion (81) are arranged to complement each other vertically. A receiving groove (84) is opened in the arrangement plate (18).
4. The tumor primary cell culture device according to claim 1, characterized in that: Each of the arrangement plates (18) has a protrusion (81) near the end of the telescopic positioning rod (71), and the protrusion (81) is a truncated cone facing the telescopic positioning rod (71).
5. The tumor primary cell culture device according to claim 1, characterized in that: The front frame (14) and the protrusion (16), and the rear frame (15) and the protrusion (16) are integrally formed.
6. The tumor primary cell culture device according to claim 3, characterized in that: The receiving frame (17) and the arranging plate (18) are respectively provided with uniformly distributed holes and cavities distributed around the receiving groove (84).