49results about How to "Pollution controllable" patented technology

The invention discloses an enterovirus type 71 (EV71) real-time fluorescent nucleic acid isothermal amplification detection kit comprising reagents such as a capturing probe, EV71 amplification primers T7 primer and nT7 primer, a EV71 detection probe, M-MLV reverse transcriptase, T7 RNA polymerase, and the like. The kit can be used for detecting EV71 RNA in throat swab or stool, and has the characteristics of high specificity, high sensitivity (reaching 10copies/reaction), low pollution (amplification product RNA can be easily degraded under natural environment), and fast detection (conventionally detection can be finished within 60min). The kit can perform important effect in clinical diagnosis of EV71 early-stage infection, and can be widely applied.

The invention discloses a simultaneous amplification and testing reagent kit for VC (vibrio cholerae). The simultaneous amplification and testing reagent kit for the VC comprises a capture probe, a VC testing primer T7, a VC testing primer nT7, a VC testing probe, an M-MLV reverse transcriptase, a T7 RNA (ribonucleic acid) polymerase and the like. The simultaneous amplification and testing reagent kit for the VC can test VC RNA in food, has the advantages of high specificity, high sensitivity (capable of reaching 100 CFU/ml), low contamination (an amplified product RNA is easy to degrade in the natural environment) and rapidness in testing (testing can be completed within 50 minutes conventionally), plays an important role in rapid testing of the VC and is wide in application prospect.

The invention discloses an RNA isothermal amplification nucleic acid detection kit aiming at Escherichia coli 0157. The kit comprises a capture probe, a pair of 0157 detection primer T7 and n T7 primer, a 0157 detection probe, M-MLV reverse transcriptase enzymes, T7 RNA polymerases and other reagents. The kit provided by the invention can detect 0157 RNA in foods, has the characteristics of high specificity, high sensitivity (which can reach 103 CFU/mL), low pollution (as the amplification product RNA is susceptible to degradation in the natural environment), accurate result (as false positives can be avoided) and quick detection (as the detection can be completed within 40 minutes conventionally ), plays an important role in the quick detection of the Escherichia coli 0157, and has a wide application prospect.

The invention discloses a production method of an amino acid secondary element fertilizer, and relates to the field of fertilizer production. The production method comprises the steps of adding an acid into animal feathers and raising temperature for hydrolysis to produce a mixed amino acid solution; then adding calcium oxide and magnesium oxide or calcium hydroxide and magnesium hydroxide into the mixed amino acid solution, and filtering to obtain a solution containing amino acid chelate secondary element; finally drying the solution to obtain the amino acid secondary element fertilizer. The production method disclosed by the invention realizes the resource utilization of waste, and has good cycle economy; the amino acid secondary element fertilizer prepared by the production method contains secondary elements of calcium and magnesium necessary for plant growth, also contains a large amount of nitrogen element necessary for plant growth, has the advantages of good water solubility, high secondary element content, good storage stability, quick plant absorption, high utilization rate of the fertilizer and the like, and has broad market prospect.

The invention discloses a primer probe for RNA isothermal amplification to detect cronobacter, a kit and a detection method. The provided primer probe for detecting specificity, the detection kit containing the primer probe, and the detection method using the detection kit to carry out RNA isothermal amplification have the characteristics of high sensitivity, strong specificity, low pollution (amplification products RNA are easily degraded in nature), and rapidness; the requirements on instruments are low; the operation is simple; the primer probe and the kit are suitable for self detection offood enterprises and basic detection mechanisms and onsite detection, in particular, milk powder rapid detection, and the safety of milk powder is guaranteed.

The invention relates to a membrane filtration pretreatment system of an on-line water quality monitor. The membrane filtration pretreatment system is characterized by comprising a filtration device, a control cabinet and the on-line water quality monitor, wherein the filtration device comprises a framework which is immersed in a mixed liquid to be sampled in a reaction cell; filtration nets are arranged around and at the bottom of the framework; a membrane component is arranged in the framework; aeration pipes are arranged below and on one side of the membrane component; an air compressor, a metering pump, a water tank and a time relay are arranged in the control cabinet; a control panel is also arranged on the control cabinet; the air compressor is communicated with the aeration pipes through air pipes; the water inlet end of the metering pump is communicated with the membrane component through a pumping pipe; the water outlet end of the metering pump is communicated with the water tank through a water pipe; the water tank is also communicated with the on-line water quality monitor through a sample injection pipe; the time relay is electrically connected with the metering pump to control the start and the stop of the metering pump; the control panel is electrically connected with the air compressor and the time relay to control the start and the stop of the air compressor and the time relay. The membrane filtration pretreatment system disclosed by the invention can be widely applied to the field of water sample pretreatment.

The invention discloses a classical swine fever virus (CSFV) real-time fluorescent nucleic acid isothermal amplification detecting kit. The kit comprises a capturing probe, CSFV detecting primers of aT7 primer and a nT7 primer, a CSFV detecting probe, M-MLV reverse transcriptase, T7 RNA polymerase and the like. The kit can detect RNA existing in pig organs, excrement and tissue culture, has the advantages of high specificity, high sensitivity (the kit can reach 100 copies per reaction), low pollution (an amplification product of RNA can be easily degraded in natural environments) and quick detection (detection conventionally needs 50 minutes), can play an important role in healthy breeding of an animal breeding industry and safe meat product monitoring, and has wide application prospects.

The invention discloses a real-time fluorescent nucleic acid thermostatic amplification detection kit of influenza virus A (H1N1) (2009). The detection kit comprises a capture probe, H1N1 (2009) detection primers of a T7 primer and an nT7 primer, an H1N1 (2009) detection probe, an M-MLV (moloney murine leukemia virus) reverse transcriptase, T7 RNA (ribonucleic acid) polymerase and the like. By adopting the kit disclosed by the invention, the H1N1 (2009) RNA in a swab can be detected; the detection kit has the characteristics of high specificity, high sensitivity (the sensitivity can achieve 100 copies/reaction), low pollution (an amplification product RNA is easily degraded in a natural environment) and fast detection, and plays an important role in clinical diagnosis of early injection of influenza A (H1N1).

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for bordetella pertussis as well as a special primer and a probe thereof, and belongs to the technical field of biomedical detection. The kit comprises a nucleic acid extracting solution, a detection solution a, a detection solution b and an SAT enzyme solution, primers and probes which are more suitable for bordetella pertussis detection are optimally designed, all components are added step by step in the detection process for step-by-step reaction, quick and accurate detection of bordetella pertussis can be achieved, and the kit is good in specificity, high in sensitivity, and the amplification product RNA is easy to degrade and does not cause sample cross contamination and environmental pollution.

The invention provides a plunger pump and a plunger motor and a closing-in-prevention forward-packet plunger sliding boot assembly thereof. The closing-in-prevention forward-packet plunger sliding boot assembly comprises a plunger and a sliding boot; the plunger comprises a ball head; the sliding boot comprises a ball socket; the ball socket is larger than a half ball; the ball diameter of the ball head is larger than the opening size of an opening of the ball socket; the ball head comprises a retraction section, wherein the radial length of the retraction section is smaller than the opening size of the opening of the ball socket so as to enable the ball head to be inserted into the ball socket; the ball head can rotate in a preset angle relative to the ball socket after being inserted into the ball socket so as to be blocked at the position of the opening of the ball socket. According to the plunger pump and the plunger motor and the closing-in-prevention forward-packet plunger sliding boot assembly thereof, the complex closing-in process is omitted through the retraction section of the ball head and the over-half-ball ball socket and accordingly the production cost is reduced; a user only needs to enable the retraction section of the ball head to be aligned to the opening of the ball socket to be inserted in and then rotates the preset angle to achieve the installation of the ball head and the ball socket; the simple is simple, the assembly is simple, the detachment and the installation are convenient, the use is convenient, the maintenance is convenient, the part pollution is convenient to control, and accordingly the service life is extended.

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for treponema pallidum 16s RNA, and a special primer and a probe thereof, and belongs to the technical field of biomedical detection. The kit provided by the invention comprises a nucleic acid extracting solution, a detection solution a, a detection solution b and an SAT enzyme solution, the primers and the probes which are more suitable for treponema pallidum 16s RNA detection are optimally designed, and the components are added step by step in the detection process to perform step-by-step reaction, so that rapid and accurate detection of treponema pallidum 16s RNA can be realized, and the kit is high in sensitivity and high in sensitivity. And the amplification product RNA is easy to degrade and does not cause sample cross contamination and environmental pollution.