49results about How to "Pollution controllable" patented technology

The invention discloses a real-time fluorescence nucleic acid constant temperature amplification detection kit of general influenza a virus (IAV). The kit comprises a capture probe, an IAV amplifier primer T7 primer, an nT7 primer, an IAV detection probe, M-MLV reverse transcriptase, T7 RNA polymerase and other reagents. The kit can detect the IAV RNA in a swab, has the advantages of being high in specificity and sensitivity (100 copies / reaction), low in pollution (due to the fact that amplification product RNA is easy to degrade in a natural environment) and quick in detection (due to the fact that detection can be finished in 50 minutes conventionally), plays an important role in clinical diagnosis of early infection of general influenza a and is wide in application prospect.

The invention provides a plunger pump as well as a plunger motor and a nosing-free reverse-package plunger piston shoe assembly thereof, wherein the nosing-free reverse-package plunger piston shoe assembly is simple in structure and convenient to dismount. The nosing-free reverse-package plunger piston shoe assembly comprises a piston shoe with a ball head and a plunger piston with a ball socket, wherein the ball socket is greater than a semi-ball; a sphere diameter of the ball head is greater than the opening caliber of the ball socket, and the ball head has a shrinking section with the radial length smaller than the opening caliber of the ball socket, so that the ball head is conveniently inserted into the ball socket; after being inserted into the ball socket, the ball head can rotate by a predetermined angle relative to the ball socket so as to be blocked at the opening of the ball socket. By virtue of the shrinking section of the ball head and the over-semi-ball setting of the ball socket, a complex nosing-free process is cancelled, and production cost is lowered; installation for the ball head and the ball socket can be realized by only needing to align the shrinking section of the ball head to the opening of the ball socket for inserting, and then rotating by the predetermined angle; the nosing-free reverse-package plunger piston shoe assembly is simple in structure, simpler to assemble, more convenient to use and maintain, can control pollution of components conveniently, so that the service life of the nosing-free reverse-package plunger piston shoe assembly is prolonged.

The invention discloses an enterovirus type 71 (EV71) real-time fluorescent nucleic acid isothermal amplification detection kit comprising reagents such as a capturing probe, EV71 amplification primers T7 primer and nT7 primer, a EV71 detection probe, M-MLV reverse transcriptase, T7 RNA polymerase, and the like. The kit can be used for detecting EV71 RNA in throat swab or stool, and has the characteristics of high specificity, high sensitivity (reaching 10copies / reaction), low pollution (amplification product RNA can be easily degraded under natural environment), and fast detection (conventionally detection can be finished within 60min). The kit can perform important effect in clinical diagnosis of EV71 early-stage infection, and can be widely applied.

The invention discloses a simultaneous amplification and testing reagent kit for VC (vibrio cholerae). The simultaneous amplification and testing reagent kit for the VC comprises a capture probe, a VC testing primer T7, a VC testing primer nT7, a VC testing probe, an M-MLV reverse transcriptase, a T7 RNA (ribonucleic acid) polymerase and the like. The simultaneous amplification and testing reagent kit for the VC can test VC RNA in food, has the advantages of high specificity, high sensitivity (capable of reaching 100 CFU / ml), low contamination (an amplified product RNA is easy to degrade in the natural environment) and rapidness in testing (testing can be completed within 50 minutes conventionally), plays an important role in rapid testing of the VC and is wide in application prospect.

The invention discloses an RNA isothermal amplification nucleic acid detection kit aiming at Escherichia coli 0157. The kit comprises a capture probe, a pair of 0157 detection primer T7 and n T7 primer, a 0157 detection probe, M-MLV reverse transcriptase enzymes, T7 RNA polymerases and other reagents. The kit provided by the invention can detect 0157 RNA in foods, has the characteristics of high specificity, high sensitivity (which can reach 103 CFU / mL), low pollution (as the amplification product RNA is susceptible to degradation in the natural environment), accurate result (as false positives can be avoided) and quick detection (as the detection can be completed within 40 minutes conventionally ), plays an important role in the quick detection of the Escherichia coli 0157, and has a wide application prospect.

The invention discloses a Coxsackie virus type A16 (CA16) real-time fluorescent nucleic acid isothermal amplification detection kit comprising reagents such as a capturing probe, CA16 amplification primers T7 primer and nT7 primer, a CA16 detection probe, M-MLV reverse transcriptase, T7 RNA polymerase, and the like. The kit can be used for detecting CA16 RNA in throat swab or stool, and has the characteristics of high specificity, high sensitivity (reaching 10copies / reaction), low pollution (amplification product RNA can be easily degraded under natural environment), and fast detection (conventionally detection can be finished within 60min). The kit can perform important effect in clinical diagnosis of CA16 early-stage infection, and can be widely applied.

The invention discloses a kit for detecting clinically common pathogenic bacteria by adopting an RNA isothermal amplification melting curve method and applications of the kit, belonging to the technical field of biological detection. The kit can detect the 16S rRNA of the following 16 clinically common pathogenic bacteria: staphylococcus aureus, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, proteus mirabilis, enterobacter aerogenes, pseudomonas fluorescens, acinetobacter baumannii, salmonella typhimurium, enterobacter cloacae, enterococcus faecium, enterococcus faecalis, bacillus proteus vulgaris, staphylococcus epidermidis, onion klebsiella and stenotrophomonas maltophilia. The kit has the features of high specificity, high sensitivity, low pollution, and detection accuracy and rapidness, plays an important role in clinical rapid identification and detection analysis of microbes, and has the wide application prospect.

The invention discloses a processing method of an instant colla corii asini cake, belonging to the technical field of food processing. The processing method comprises the follows processing steps of: crushing and mixing materials such as colla corii asini, and the like before decocting, then packaging in a package box, heating the materials in the packet box or injecting preparation liquid into the packet box and then heating to melt and mould the materials in the box, dividing and packaging after cooling. The processing method of instant colla corii asini cake is simple and reliable, and effectively solves the technical problems in the traditional processing method that the materials are wasted because the colla corii asini paste is adhered to processing devices, the processing devices are difficult to wash and automatic flow line production cannot be realized; and the processing efficiency is high, the processing cost can be efficiently reduced and pollution in processing link can be controlled easily.

The embodiment of the invention discloses a micro-fluidic in-situ SERS detection method for trace mercury ion detection, which comprises the following steps: introducing a mixed solution of silver nitrate and sodium citrate into a micro-fluidic chip interface 1, and directly preparing a silver nano aggregate as an SERS substrate in a micro-fluidic pipeline by adopting a laser micro-area photo-reduction method; introducing a NaI solution into an interface 2 of the micro-fluidic chip, and carrying out I-modification on the silver nano aggregate; a crystal violet solution is introduced into an interface 3, and an SERS signal of the silver nano aggregate is collected; a sample Hg < 2 + > solution is introduced into an interface 4, and SERS signals are collected again in situ; counting the decrease rate of the SERS intensity of the crystal violet molecules before and after the action of the Hg < 2 + > to realize sensing of the Hg < 2 + >; furthermore, repeating the steps are repeated to measure the Hg < 2 + > samples with different concentrations, thereby obtaining the function relation between the logarithm of the Hg < 2 + > concentration and the SERS intensity reduction rate. The method provides a basis for quantitative detection of the Hg < 2 + >.

The invention discloses a primer probe for RNA isothermal amplification to detect cronobacter, a kit and a detection method. The provided primer probe for detecting specificity, the detection kit containing the primer probe, and the detection method using the detection kit to carry out RNA isothermal amplification have the characteristics of high sensitivity, strong specificity, low pollution (amplification products RNA are easily degraded in nature), and rapidness; the requirements on instruments are low; the operation is simple; the primer probe and the kit are suitable for self detection offood enterprises and basic detection mechanisms and onsite detection, in particular, milk powder rapid detection, and the safety of milk powder is guaranteed.

The invention relates to a real-time fluorescent nucleic acid constant temperature amplification (SAT) detection kit for bird-mycobacterium avium complex, and a specific primer and a probe thereof. The specific region of 16S rRNA is adopted as a detection target. The specific primer pair and the probe have the advantages of high specificity and high sensitivity, and the kit can detect the bird-MAC RNA in a clinic sample isolate strain, has the characteristics of high specificity, high sensitivity, low pollution and fastness in detection, and plays a role in the clinic diagnosis of the bird-MAC.

The invention relates to a membrane filtration pretreatment system of an on-line water quality monitor. The membrane filtration pretreatment system is characterized by comprising a filtration device, a control cabinet and the on-line water quality monitor, wherein the filtration device comprises a framework which is immersed in a mixed liquid to be sampled in a reaction cell; filtration nets are arranged around and at the bottom of the framework; a membrane component is arranged in the framework; aeration pipes are arranged below and on one side of the membrane component; an air compressor, a metering pump, a water tank and a time relay are arranged in the control cabinet; a control panel is also arranged on the control cabinet; the air compressor is communicated with the aeration pipes through air pipes; the water inlet end of the metering pump is communicated with the membrane component through a pumping pipe; the water outlet end of the metering pump is communicated with the water tank through a water pipe; the water tank is also communicated with the on-line water quality monitor through a sample injection pipe; the time relay is electrically connected with the metering pump to control the start and the stop of the metering pump; the control panel is electrically connected with the air compressor and the time relay to control the start and the stop of the air compressor and the time relay. The membrane filtration pretreatment system disclosed by the invention can be widely applied to the field of water sample pretreatment.

The invention discloses a classical swine fever virus (CSFV) real-time fluorescent nucleic acid isothermal amplification detecting kit. The kit comprises a capturing probe, CSFV detecting primers of aT7 primer and a nT7 primer, a CSFV detecting probe, M-MLV reverse transcriptase, T7 RNA polymerase and the like. The kit can detect RNA existing in pig organs, excrement and tissue culture, has the advantages of high specificity, high sensitivity (the kit can reach 100 copies per reaction), low pollution (an amplification product of RNA can be easily degraded in natural environments) and quick detection (detection conventionally needs 50 minutes), can play an important role in healthy breeding of an animal breeding industry and safe meat product monitoring, and has wide application prospects.

The invention discloses a real-time fluorescent nucleic acid constant-temperature amplification and detection kit for human seasonal influenza viruses H1N1 (HuH1N1). The real-time fluorescent nucleic acid constant-temperature amplification and detection kit comprises a capturing probe, a HuH1N1 detection primer T7, a primer nT7, a HuH1N1 detection probe, an M-MLV reverse transcriptase, T7 RNA (ribonucleic acid) polymerase and the like. The kit disclosed by the invention can detect HuH1N1 RNA in a swab, has the characteristics of high specificity, high sensitivity (which is up to 100copies / reaction), low pollution (due to easy degradation of the RNA of amplification products under a natural environment) and quickness in detection ( due to detection normally implemented within 50 minutes), plays an important role in clinical diagnosis of early infection of the human seasonal influenza and has a large application prospect.

The invention discloses a real-time fluorescent nucleic acid thermostatic amplification detection kit of influenza virus A (H1N1) (2009). The detection kit comprises a capture probe, H1N1 (2009) detection primers of a T7 primer and an nT7 primer, an H1N1 (2009) detection probe, an M-MLV (moloney murine leukemia virus) reverse transcriptase, T7 RNA (ribonucleic acid) polymerase and the like. By adopting the kit disclosed by the invention, the H1N1 (2009) RNA in a swab can be detected; the detection kit has the characteristics of high specificity, high sensitivity (the sensitivity can achieve 100 copies / reaction), low pollution (an amplification product RNA is easily degraded in a natural environment) and fast detection, and plays an important role in clinical diagnosis of early injection of influenza A (H1N1).

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for bordetella pertussis as well as a special primer and a probe thereof, and belongs to the technical field of biomedical detection. The kit comprises a nucleic acid extracting solution, a detection solution a, a detection solution b and an SAT enzyme solution, primers and probes which are more suitable for bordetella pertussis detection are optimally designed, all components are added step by step in the detection process for step-by-step reaction, quick and accurate detection of bordetella pertussis can be achieved, and the kit is good in specificity, high in sensitivity, and the amplification product RNA is easy to degrade and does not cause sample cross contamination and environmental pollution.

The invention relates to a comprehensive utilization method of organic hazardous waste salt slag. The comprehensive utilization method comprises the following specific steps: carbonizing dried organichazardous waste salt slag in a tubular furnace in a nitrogen atmosphere; grinding the carbonization product, adding water to dissolve the carbonization product, and performing filtering to obtain a carbonization product and a filtrate; adding an activating agent and water into the carbonization product, uniformly mixing, standing, filtering, drying a filter cake obtained by filtering for later use, collecting and uniformly treating the obtained filtrate, and activating the dried filter cake in a tubular furnace to obtain activated carbon; and evaporating the obtained filtrate to obtain pure salt. Industrial waste can be treated according to local conditions, water and soil resources are effectively protected, and environmental pollution is controlled. The method has important environmental, economic and social benefits.

The invention relates to a process and apparatus for dry suspension sintering flash smelting of ferronickel, belonging to the field of ferronickel production processes and apparatuses. The process comprises the following steps: 1) drying; 2) grinding; 3) multi-stage suspension pre-reduction; 4) sintering reduction; and 5) flash furnace smelting. Flue gas with waste heat from flash furnace successively enters a sintering furnace and a multi-stage suspension pre-reduction system; the heat and a reducing agent in the flue gas act on the links of pre-reduction and sintering reduction, so the wasteheat is utilized, and energy consumption is reduced; and mineral materials in the flue gas settle down in a preheating system and then return to a melting system, so productivity is enhanced and theemissions of pollutants are lowered. Material flow in the whole system operates in a closed loop, and exhaust gas is purified and then discharged after reaching the standard. The apparatus comprises adrying device, a grinding device and a dry suspension sintering device, wherein the drying device, the grinding device and the dry suspension sintering device are sequentially connected. The processand apparatus of the invention has the advantages of low investment cost, obvious energy saving effect, no consumption of electricity and coke, greatly-reduced power consumption, substantially-loweredcost for reducing agents, full utilization of exhaust gas and waste heat and high automation degree.

The invention provides a t thin film deposition method and device. The method comprises the following steps that S1, a first precursor and makeup gas are led into a buffer cavity connected with a reaction cavity, the makeup gas is blowing gas or a second precursor, the makeup gas is mixed with the first precursor in a buffer cavity, and after mixing of the makeup gas and the first precursor, a mixture flows into a reaction cavity; S2, leading of the first precursor into the buffer cavity is stopped, the blowing gas and the second precursor are led into the buffer cavity, a valve of a plasma cleaning source, the buffer cavity and the reaction cavity are blown in sequence; and S3, radio frequency power is loaded in the reaction cavity, the second precursor in the reaction cavity is stimulated to form plasmas, the plasmas react with the first precursor, and a thin film is formed on a wafer. Through the method and device, the distribution uniformity of deposited thin film thickness can beimproved.

The invention discloses a realtime fluorescent nucleic acid constant temperature amplification detection kit of human cytomegalovirus (HCMV). The detection kit comprises a capture probe, a HCMVT7 primer, an nT7 primer, a HCMV detection probe, M-MLV reverse transcriptase and T7RNA polymerase. The provided method can high specifically, high sensitively, and rapidly carry out nucleic acid amplification detection on samples containing HCMV such as urine, milk, blood, and the like, and little pollution is generated. The provided kit and method can accurately, rapidly, and conveniently carrying out qualitative detection on HCMV; diagnose the virus infection, monitor and predict epidemicity of HCMV, helps the auxiliary diagnosis of virus infection, and observe the drug therapeutic effect.

The invention discloses a simultaneous amplification and testing reagent kit for VC (vibrio cholerae). The simultaneous amplification and testing reagent kit for the VC comprises a capture probe, a VC testing primer T7, a VC testing primer nT7, a VC testing probe, an M-MLV reverse transcriptase, a T7 RNA (ribonucleic acid) polymerase and the like. The simultaneous amplification and testing reagent kit for the VC can test VC RNA in food, has the advantages of high specificity, high sensitivity (capable of reaching 100 CFU / ml), low contamination (an amplified product RNA is easy to degrade in the natural environment) and rapidness in testing (testing can be completed within 50 minutes conventionally), plays an important role in rapid testing of the VC and is wide in application prospect.

The invention discloses a Coxsackie virus type A16 (CA16) real-time fluorescent nucleic acid isothermal amplification detection kit comprising reagents such as a capturing probe, CA16 amplification primers T7 primer and nT7 primer, a CA16 detection probe, M-MLV reverse transcriptase, T7 RNA polymerase, and the like. The kit can be used for detecting CA16 RNA in throat swab or stool, and has the characteristics of high specificity, high sensitivity (reaching 10copies / reaction), low pollution (amplification product RNA can be easily degraded under natural environment), and fast detection (conventionally detection can be finished within 60min). The kit can perform important effect in clinical diagnosis of CA16 early-stage infection, and can be widely applied.

The invention discloses a processing method of an instant colla corii asini cake, belonging to the technical field of food processing. The processing method comprises the follows processing steps of: crushing and mixing materials such as colla corii asini, and the like before decocting, then packaging in a package box, heating the materials in the packet box or injecting preparation liquid into the packet box and then heating to melt and mould the materials in the box, dividing and packaging after cooling. The processing method of instant colla corii asini cake is simple and reliable, and effectively solves the technical problems in the traditional processing method that the materials are wasted because the colla corii asini paste is adhered to processing devices, the processing devices are difficult to wash and automatic flow line production cannot be realized; and the processing efficiency is high, the processing cost can be efficiently reduced and pollution in processing link can be controlled easily.

The invention discloses a detection method for detecting difference of nucleic acid fragments and belongs to the technical field of nucleic acid determination. According to the invention, a designing scheme of a related primer set and a probe for difference of the nucleic acid fragments is provided; a to-be-detected sample nucleic acid is taken as a template; the primer set is applied for amplified reaction for 80 minutes under constant-temperature reaction conditions of 55-90 DEG C; then, visual judgment can be performed according to turbidity or color changing by utilizing fluorescent dye for dyeing, thereby identifying the difference of the nucleic acid fragments. The detection method is simple and convenient to operate, quick in detection and capable of completing all operations including detection without expensive nucleic acid amplifying instruments; and the detection efficiency is improved.

The invention provides a plunger pump and a plunger motor and a closing-in-prevention forward-packet plunger sliding boot assembly thereof. The closing-in-prevention forward-packet plunger sliding boot assembly comprises a plunger and a sliding boot; the plunger comprises a ball head; the sliding boot comprises a ball socket; the ball socket is larger than a half ball; the ball diameter of the ball head is larger than the opening size of an opening of the ball socket; the ball head comprises a retraction section, wherein the radial length of the retraction section is smaller than the opening size of the opening of the ball socket so as to enable the ball head to be inserted into the ball socket; the ball head can rotate in a preset angle relative to the ball socket after being inserted into the ball socket so as to be blocked at the position of the opening of the ball socket. According to the plunger pump and the plunger motor and the closing-in-prevention forward-packet plunger sliding boot assembly thereof, the complex closing-in process is omitted through the retraction section of the ball head and the over-half-ball ball socket and accordingly the production cost is reduced; a user only needs to enable the retraction section of the ball head to be aligned to the opening of the ball socket to be inserted in and then rotates the preset angle to achieve the installation of the ball head and the ball socket; the simple is simple, the assembly is simple, the detachment and the installation are convenient, the use is convenient, the maintenance is convenient, the part pollution is convenient to control, and accordingly the service life is extended.

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for treponema pallidum 16s RNA, and a special primer and a probe thereof, and belongs to the technical field of biomedical detection. The kit provided by the invention comprises a nucleic acid extracting solution, a detection solution a, a detection solution b and an SAT enzyme solution, the primers and the probes which are more suitable for treponema pallidum 16s RNA detection are optimally designed, and the components are added step by step in the detection process to perform step-by-step reaction, so that rapid and accurate detection of treponema pallidum 16s RNA can be realized, and the kit is high in sensitivity and high in sensitivity. And the amplification product RNA is easy to degrade and does not cause sample cross contamination and environmental pollution.

The invention discloses a real-time fluorescence nucleic acid constant temperature amplification detection kit of general influenza a virus (IAV). The kit comprises a capture probe, an IAV amplifier primer T7 primer, an nT7 primer, an IAV detection probe, M-MLV reverse transcriptase, T7 RNA polymerase and other reagents. The kit can detect the IAV RNA in a swab, has the advantages of being high in specificity and sensitivity (100 copies / reaction), low in pollution (due to the fact that amplification product RNA is easy to degrade in a natural environment) and quick in detection (due to the fact that detection can be finished in 50 minutes conventionally), plays an important role in clinical diagnosis of early infection of general influenza a and is wide in application prospect.

The invention discloses a novel lead-free X-ray protection material and a preparation method and an application thereof. The novel lead-free X-ray protection material comprises a base material and coatings arranged on two sides of the base material in a coating manner, the base material is an X-ray protection fiber net layer, and the coatings are prepared from the following components in parts by weight: 1-2 parts of SEBS synthetic rubber, 2-4 parts of white oil, 7-8 parts of bismuth trioxide, 0.01-0.1 part of ethylene bisstearamide, 0.01-0.02 part of tetra-[beta-(3, 5-ditertbutyl-4-hydroxy-phenyl)-propionic acid]-pentaerythritol ester, 0.01-0.03 part of tri [2,4-ditertbutyl-phenyl]-phosphite, 1-3 parts of TPU resin and 0.5-0.8 part of industrial toner. The novel lead-free X-ray protective material is used for manufacturing medical protective clothing, protective gloves, home protective clothing and maternity clothes, the material is free of carcinogenicity, the anti-tensile effect of the material is improved, the anti-deformation performance of the material is bettered, and the protection effect of the protection material is improved by spraying particles.