The invention belongs to the technical field of medicines, and particularly relates to ruby regeneration ointment and a preparation process thereof. The ruby regeneration ointment is prepared from thefollowing raw materials in parts by weight: 50 to 150 parts of calamine, 30 to 120 parts of amber, 30 to 120 parts of rheum officinale, 30 to 120 parts of dragon's blood, 30 to 120 parts of radix angelicae, 100 to 300 parts of calcined gypsum, 30 to 90 parts of natural borneol, 20 to 80 parts of cuttlebone, 30 to 120 parts of fructus gardenia, 1900 to 2100 parts of vaseline, 25 to 35 parts of teaoil and 100 to 350 parts of lanolin. The ointment is prepared from the following components in parts by weight: 1900 to 2100 parts of vaseline, 25 to 35 parts of tea oil and 100 to 350 parts of woolfat. The process comprises the following steps: respectively vibrating, grinding and breaking walls of the raw materials into ultrafine powder, and performing sieving with a 200-mesh sieve for later use; and weighing, mixing, heating and cooling the vaseline and the tea oil to 60-70 DEG C, adding and stirring the calamine, the amber, the rheum officinale, the radix angelicae, the calcined gypsum and the fructus gardenia, adding and stirring the cuttlebone, the dragon's blood and the natural borneol, and cooling the mixture to the normal temperature to obtain the ointment. The ointment containsanti-inflammatory, detumescent, analgesia, necrotic tissue-removing, granulation-promotion and hemostatic drugs, is remarkable in clinical effect by applying a wet healing method, and is suitable fortreating bedsores, open wounds, wounds of old people, diabetic feet, non-healing burns and scalds and the like.