A dna microarray chip and its detection method and its application in the detection of cyp3a4, cyp3a5 and mdr1 gene polymorphisms

A microarray chip, gene technology, applied to the application of CYP3A5 and MDR1 gene polymorphism, DNA microarray chip and its detection, detection of CYP3A4 field, can solve the problem of time-consuming, low detection throughput, practical clinical detection and diagnosis It is easy to operate, simple to prepare and realize the effect of personalized medicine.
CN101481736BInactive Publication Date: 2011-12-28SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2011-12-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a DNA microarray chip, its detection method and its application in detecting CYP3A4, CYP3A5 and MDR1 gene polymorphisms. The chip includes a solid phase carrier and a probe, and the nucleotide sequence of the probe is as the probe The nucleotide sequences are shown in SEQ ID NO: 1 to SEQ ID NO: 49. The present invention first loads the probe on the solid phase carrier, then labels and prepares the target nucleotide sequence of the sample to be tested, then hybridizes the target nucleotide sequence with the probe on the chip, and finally analyzes the hybridization result The detection can obtain the information of CYP3A4, CYP3A5 and MDR1 genes in the sample to be tested. The chip of the present invention can simultaneously detect multiple genes on multiple samples at one time, with simple operation and high accuracy of results, and a large amount of genetic information of patients can be obtained through one detection, which can be used to guide the formulation of rational drug regimens and realize individualization medical.
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Description

technical field

[0001] The invention relates to medical in vitro diagnostic technology, in particular to a DNA microarray chip and its detection method and its application in detecting CYP3A4, CYP3A5 and MDR1 gene polymorphisms. Background technique

[0002] Organ transplantation is the most effective method for the treatment of end-stage liver disease. The widespread use of clinical immunosuppressants tacrolimus, cyclosporine and rapamycin has improved the clinical effect of organ transplantation. At the same time, its toxic and side effects have also attracted increasing attention.

[0003] In clinical application, it is very likely that the insufficient dosage of the drug will lead to "insufficient immunosuppression", which will lead to the immune rejection of the graft and lead to graft failure, or the "excessive immunosuppression" caused by excessive drug administration will lead to the occurrence of liver and kidney toxicity, infection and tumor. At present, the tradit...

Claims

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