Preservation, activation and rapid propagation method of extreme-drought-tolerant syntrichia caninervis single plant cloning system
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A technology of Erythritis dentatus, extreme drought tolerance, applied in the field of bioengineering
Inactive Publication Date: 2014-03-12
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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Further searches did not find any reports on the preservation, activation and rapid propagation of single clones of E. denticola
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Embodiment 1
[0045] Tissue culture of Erythrosa dentata:
[0046] a. After washing the Erythrodonticum collected in the field, peel off the rhizomes, stems and leaves, strip off the bright green leaves of the rehydrated wild Erythras denticulae, shake and wash them with sterile water for 5 times, and then inoculate to the improved The composition of the Knop solid medium is 0.25g / L KH 2 PO 4 , KCl and MgSO 4 ·7H 2 O, 1g / L Ca(NO 3 ) 2 .4H 2 O and 0.0125g / L FeSO 4 ·7H 2 O composition, pH value 6.5, placed in a light incubator for cultivation, culture temperature is 25°C during the day, 15°C at night, the light cycle is 14h during the day, 10h at night, the light intensity is 4000lx, the humidity is 50%, culture 2-3 week, a large number of protonema produced;
[0047] b, the protocelium produced in step a is subcultured to the fresh improved Knop solid medium as 0.25g / L KH 2 PO 4 , KCl and MgSO 4 ·7H 2 O, 1g / L Ca(NO 3 ) 2 .4H 2 O and 0.0125g / LFeSO 4 ·7H 2 On O, the pH value ...
Embodiment 2
[0056] Tissue culture of Erythrosa dentata:
[0057] a. After washing the Erythrodonticum collected in the field, peel off the rhizomes, stems and leaves, strip off the bright green leaves of the rehydrated wild Erythras denticulae, shake and wash them with sterile water for 5 times, and then inoculate to the improved The composition of the Knop solid medium is 0.25g / L KH 2 PO 4 , KCl and MgSO 4·7H 2 O, 1g / L Ca(NO 3 ) 2 .4H 2 O and 0.0125g / L FeSO 4 ·7H 2 O composition, pH value 6.5, placed in a light incubator for cultivation, culture temperature is 25°C during the day, 15°C at night, the light cycle is 14h during the day, 10h at night, the light intensity is 4000lx, the humidity is 50%, culture 2-3 week, a large number of protonema produced;
[0058] b, the protocelium produced in step a is subcultured to the fresh improved Knop solid medium as 0.25g / L KH 2 PO 4 , KCl and MgSO 4 ·7H 2 O, 1g / L Ca(NO 3 ) 2 .4H 2 O and 0.0125g / LFeSO 4 ·7H 2 On O, the pH value i...
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Abstract
The invention relates to a preservation, activation and rapid propagation method of extreme-drought-tolerant syntrichia caninervis single plant cloning system, the method allows peeled rehydrated leaves to be inoculated to an improved Knop solid medium, and then switched to a fresh improved Knop solid medium to generate a large number of single plant cloning regenerated plantlets; the regenerated plantlets are put in an ultra low temperature refrigerator for long-term preservation after being slowly dried; after the preserved materials undergo slow rehydration, enlargement culture is carried out to obtain large batches of pure material. The method in the invention assists in rapidly cloning large batches of single plant cloning systems in 1-2 months from a leaf or explant, providing continuous pure materials for syntrichia caninervis as a drought resisting research mode plant, and simultaneously providing methods for "desert carpet engineering". A sustaining obtain and enlargement of syntrichia caninervis single plant cloning system materals is realized. The method in the invention has the advantages of simple method, low cost and short period, and growth degrees of the obtained single plant cloning system are consistent.
Description
technical field [0001] The invention relates to a method for preserving, activating and rapidly multiplying an extremely drought-resistant single-plant clonal line of Erythra dentatus, belonging to the technical field of bioengineering. Background technique [0002] Aridification is a serious global problem. Drought not only caused heavy losses in agriculture, but also exacerbated the deterioration of ecological environment, land desertification and soil erosion. The direct way to solve this problem is to save water effectively. Plants are the basis for human survival. How can plants resist drought and save water? This requires starting from drought-tolerant plants to gain an in-depth understanding of the drought-tolerant mechanism and regulation mechanism of plants, so as to improve the drought-tolerant ability of plants. With the rapid development and improvement of genetic engineering and molecular biology techniques, the search for drought-resistant genetic resources ...
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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 张道远杨红兰李小双张元明
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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