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Method and kit for cancer diagnosis

A technology for prostate cancer and antigen, applied in the field of cancer diagnosis and kits, can solve problems such as unclear physiological functions

Inactive Publication Date: 2013-01-09
PROSTASOM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The prostatic corpuscle has many different biological activities, but its physiological function remains unclear

Method used

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  • Method and kit for cancer diagnosis
  • Method and kit for cancer diagnosis
  • Method and kit for cancer diagnosis

Examples

Experimental program
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Embodiment Construction

[0027] Sample preparation

[0028] Provide semen samples from 79 patients with no known prostate cancer (based on PSA value and clinical and recall information) and 10 patients with confirmed prostate cancer.

[0029] In order to separate sperm and seminal plasma, human semen was incubated at room temperature for about 30 minutes and centrifuged at 1000×g for 20 minutes at a temperature of 20°C to obtain seminal fluid from human semen. The semen slurry was thus prepared for the following flow cytometry analysis.

[0030] Urine, prostate secretions and heparinized blood samples were centrifuged at 2500×g for 10 minutes at a temperature of 20°C. The supernatants from prostate secretions, urine, and heparinized blood were prepared separately for flow cytometry analysis. In addition, the plasma obtained by centrifugation was tested with ELISA on the solid phase and dot blotting / immunoblotting with antibodies specific to prostate corpuscles on nitrocellulose paper (see ELISA and immuno...

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Abstract

The invention relates to a method for diagnosing or providing a prognosis of a subject suspected of suffering from prostate cancer, comprising in vitro detection of prostasomes and quantification of prostasomal expression of at least one antigen chosen from the group consisting of CD13, CD59, CD10, CD26 CD142, CD143 and MHC I, and comparing said quantified expression value with a reference value for the respective antigen derived from healthy subject(s). Quotients between said antigens may moreover be made use of. Detection may be by way of flow cytometry or ELISA. A kit for use in diagnosis or providing a prognosis of a subject suspected of suffering from prostate cancer is furthermore provided.

Description

Technical field [0001] The present invention relates to methods and kits for diagnosing and providing prognosis for subjects suspected of having prostate cancer. Background technique [0002] Cancer is one of the most common fatal diseases. Despite its new progress in diagnosis and treatment, there are still a large number of deaths every year. [0003] For example, prostate cancer is the most common cancer disease among the ten diagnosed cancer diseases in men. Prostate cancer exhibits symptoms derived from local tumors or tumor metastatic spread, such as excretion dysfunction or bone pain, and is often diagnosed The disease has reached an advanced stage. Sometimes prostate cancer is accidentally discovered during a digital rectal examination or histological examination of tissues obtained during surgery on men with benign prostatic hyperplasia. [0004] The measurement of prostate specific antigen (PSA) has changed the diagnosis of prostate cancer, making more cases detected in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57434G01N2800/52
Inventor 鲁内·埃利阿松尼尔斯·埃格贝里莱纳·卡尔森冈纳·伦奎斯特安德斯·拉尔森戈埃兰·伦奎斯特
Owner PROSTASOM
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