A kind of preparation method of Periplaneta americana allergen preparation

Abstract

The present invention provides a periplaneta americana allergen preparation production method, which comprises: (1) inactivating periplaneta americana in a refrigerator, removing intestinal tracts, washing with 75% ethanol, carrying out air-drying, and placing in the refrigerator so as to be spare; (2) taking an appropriate amount of the frozen periplaneta americana, and grinding into powder in liquid nitrogen; (3) degreasing with acetone, placing into the refrigerator to freeze, and carrying out freeze-drying in a vacuum freeze dryer to obtain powder; (4) weighing the periplaneta americana powder, adding an extraction solution, carrying out centrifugation, and sucking the supernatant, wherein the supernatant is a periplaneta americana allergen extract; (5) adopting specific antibodies to identify the content and the proportion of the main allergen in the periplaneta americana allergen crude extract; and (6) according to the identification result in the step (5), adding recombinant protein expressed by yeast or insects. With the periplaneta americana allergen crude extract extracted by using the method, the main allergen protein of the periplaneta americana can be obtained to the maximal degree, the extract has good repeatability, and the error is less than 30%.

Description

technical field

[0001] The invention relates to the technical field of preparation of allergen preparations, in particular to a preparation method of Periplaneta americana allergen preparations. Background technique

[0002] Allergic diseases are one of the major human diseases. Its incidence rate is currently estimated to account for about 22% of the world's population, and is increasing at a rate of more than 1% per year, and the incidence rate of children is the most obvious increase. Its harmfulness cannot be simply measured by its mortality rate, but the economic burden caused by the high attack frequency of the disease should be considered. Because allergic diseases mostly occur in young people, their impact on human society and economy is even more serious. In the process of industrialization transformation in my country, its incidence rate has increased significantly, seriously affecting the quality of life and work efficiency of the affected population in my count...

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