Rapid propagation method for xanthoceras sorbifolia bunge tissue during culturing

A technology of tissue culture and sorbifolium, applied in the field of plant tissue culture, can solve the problems of difficult rooting, low callus induction rate, browning, etc.

Inactive Publication Date: 2015-01-21
杨业容
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a kind of woody plant, there are many problems in the process of tissue culture, such as low callus induction rate, browning, and difficulty in rooting. Its research is still in the early stage, and a complete in vitro rapid propagation sy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Treatment of explants: Select the mature embryos of X. sorbifolium as explants, place them in warm water at 60°C and cool them down to room temperature for 5 hours, and then disinfect them with 0.1% mercuric chloride solution containing 0.01% Tween-20 2min, washed 5 times with sterile water, peeled off the seed coat, soaked in 75% ethanol solution for 20s, rinsed 5 times with sterile water, and then sterilized with 0.1% mercuric chloride solution containing 0.01% Tween-20 for 2 minutes. Bacterial water was washed 5 times before inoculation, and the success rate was 65%.

[0020] 2. Callus induction: cut the sterilized seed embryo into about 3-5mm 3 The tissue pieces were inoculated on the induction medium for callus induction culture. After inoculation, they were first cultured in total darkness at 25°C for 20 days, and then placed in light for 10 hours a day, with a light intensity of 1500x and a culture temperature of 25°C. Culture under the conditions until callu...

Embodiment 2

[0026] 1. Treatment of explants: Select the mature embryos of X. sorbifolium as explants, place them in warm water at 70°C and cool them down to room temperature for 5 hours, and then sterilize them with 0.1% mercuric chloride solution containing 0.03% Tween-20 5min, washed 3 times with sterile water, peeled off the seed coat, soaked in 78% ethanol solution for 25s, rinsed 5 times with sterile water, and then sterilized with 0.1% mercuric chloride solution containing 0.03% Tween-20 for 5min. Bacterial water was washed 5 times before inoculation, and the success rate was 73%.

[0027] 2. Callus induction: cut the sterilized seed embryo into about 3-5mm 3 The tissue pieces were inoculated on the induction medium for callus induction culture. After inoculation, they were cultured in total darkness at 28°C for 23 days, and then placed in the light for 11 hours a day, with a light intensity of 1500x and a culture temperature of 28°C. Culture under the conditions until callus is fo...

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PUM

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Abstract

The invention discloses a rapid propagation method for the xanthoceras sorbifolia bunge tissue during culturing, and aims to solve problems about low germination rate, low grafting survival rate and difficulty in cutting rooting of the xanthoceras sorbifolia bunge by a plant cell engineering technology are solved. According to the method, xanthoceras sorbifolia bunge test-tube plantlets with excellent characteristics required by people are obtained in a short time. With xanthoceras sorbifolia bunge embyos as explants, rapid propagation for the xanthoceras sorbifolia bunge tissue during culturing is realized by callus induction, subculture, adventitious shoot differentiation, rooting culture, acclimatizating and transplanting of the test-tube plantlets and other phases; a large amount of high-quality and vigorous seedlings are provided for vigorously developing large-scale planting of the xanthoceras sorbifolia bunge; besides, experiment materials are provided for research on genetic engineering of the xanthoceras sorbifolia bunge, such as genetic transformation and the like.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of tissue culture of sorbifolia. Background technique [0002] Xingoguan fruit ( Xanthoceras sorbifolia Bunge) is a deciduous shrub or small tree belonging to the Sapindaceae genus S. xanthus. It is an extremely precious woody medicinal and oily plant in my country. The trunk and branches of sorbifolium sorbifolium are called sorbifolium, sweet in taste and cool in nature. It has curative effects such as dispelling rheumatism, reducing swelling and pain, and astringing dry yellow water. of traditional medicines. In addition, the fatty acid content of Xanthania sorbifolium oil is very rich, the content of fatty acid is close to 60%, and some varieties have higher content, and the content of unsaturated fatty acid is more than 90%. Therefore, Xanthania sorbifolium is a high-quality energy plant. [0003] As a k...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨业容
Owner 杨业容
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